CURRICULUM VITAE (Updated: 6 /1/2020) NAME Michael Peter Sarras, Jr
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Neurogenic Decisions Require a Cell Cycle Independent Function of The
RESEARCH ARTICLE Neurogenic decisions require a cell cycle independent function of the CDC25B phosphatase Fre´ de´ ric Bonnet1†, Angie Molina1†, Me´ lanie Roussat1, Manon Azais2, Sophie Bel-Vialar1, Jacques Gautrais2, Fabienne Pituello1*, Eric Agius1* 1Centre de Biologie du De´veloppement, Centre de Biologie Inte´grative, Universite´ de Toulouse, CNRS, UPS, Toulouse, France; 2Centre de Recherches sur la Cognition Animale, Centre de Biologie Inte´grative., Universite´ de Toulouse, CNRS, UPS, Toulouse, France Abstract A fundamental issue in developmental biology and in organ homeostasis is understanding the molecular mechanisms governing the balance between stem cell maintenance and differentiation into a specific lineage. Accumulating data suggest that cell cycle dynamics play a major role in the regulation of this balance. Here we show that the G2/M cell cycle regulator CDC25B phosphatase is required in mammals to finely tune neuronal production in the neural tube. We show that in chick neural progenitors, CDC25B activity favors fast nuclei departure from the apical surface in early G1, stimulates neurogenic divisions and promotes neuronal differentiation. We design a mathematical model showing that within a limited period of time, cell cycle length modifications cannot account for changes in the ratio of the mode of division. Using a CDC25B point mutation that cannot interact with CDK, we show that part of CDC25B activity is independent *For correspondence: of its action on the cell cycle. [email protected] (FP); [email protected] (EA) †These authors contributed equally to this work Introduction In multicellular organisms, managing the development, homeostasis and regeneration of tissues Competing interests: The requires the tight control of self-renewal and differentiation of stem/progenitor cells. -
New Editor on Journal of Cell Science Michael Way (Editor-In-Chief)
© 2019. Published by The Company of Biologists Ltd | Journal of Cell Science (2019) 132, jcs229740. doi:10.1242/jcs.229740 EDITORIAL New Editor on Journal of Cell Science Michael Way (Editor-in-Chief) As someone who has worked on things related to the actin cytoskeleton my whole research career, the nucleus was not something I paid much attention to. Yes, there were scattered historical reports of actin in the nucleus long before I started my PhD, but no one believed actin was really there of course – it was all an artefact of fixation, you know. Nuclear actin was taboo and no one talked about it at the meetings I went to as a student and postdoc. How wrong we were – today nuclear actin is alive and kicking, although there are definitely more questions than answers concerning what it is actually doing there. We now appreciate that the nucleus contains a wide assortment of proteins associated with the cytoplasmic actin cytoskeleton including myosin motors and actin nucleators such as the Arp2/3 complex. In addition, it should not be forgotten that many chromatin-associated complexes including SWI/SNF and INO80/ SWR also contain multiple actin-related proteins, as well as actin itself. It strikes me that maybe we should all be paying more attention to the nucleus and not just because it contains my favourite proteins! Maybe that’s why, in recent years, we’ve been seeing more submissions to JCS that are focused on different aspects of the nucleus and that traditionally appeared in journals with ‘molecular’ in their titles. -
Theory of Cytoskeletal Reorganization During Crosslinker-Mediated Mitotic Spindle Assembly
bioRxiv preprint doi: https://doi.org/10.1101/419135; this version posted March 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly A. R. Lamson, C. J. Edelmaier, M. A. Glaser, and M. D. Betterton Abstract Cells grow, move, and respond to outside stimuli by large-scale cytoskeletal reorganization. A prototypical example of cytoskeletal remodeling is mitotic spindle assembly, during which micro- tubules nucleate, undergo dynamic instability, bundle, and organize into a bipolar spindle. Key mech- anisms of this process include regulated filament polymerization, crosslinking, and motor-protein activity. Remarkably, using passive crosslinkers, fission yeast can assemble a bipolar spindle in the absence of motor proteins. We develop a torque-balance model that describes this reorganization due to dynamic microtubule bundles, spindle-pole bodies, the nuclear envelope, and passive crosslink- ers to predict spindle-assembly dynamics. We compare these results to those obtained with kinetic Monte Carlo-Brownian dynamics simulations, which include crosslinker-binding kinetics and other stochastic effects. Our results show that rapid crosslinker reorganization to microtubule overlaps facilitates crosslinker-driven spindle assembly, a testable prediction for future experiments. Combin- ing these two modeling techniques, we illustrate a general method for studying cytoskeletal network reorganization. 1 bioRxiv preprint doi: https://doi.org/10.1101/419135; this version posted March 1, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. -
Journal of Cell Science & Therapy
Journal of Cell Science & Therapy 2021 Conference Announcement Mark on Your Calendar, Stem Cell 2021 is coming soon!! Ahmed Hegazi Pursued by the Successful Completion of the Stem Cell discuss the latest developments in the field of Stem Cell and Conference, we are facilitating its next version “International Regenerative Medicine as well. Current studies of Stem cell Conference on Stem Cell” in Osaka, Japan on March 16-17, are examining how undifferentiated organisms might be 2021. utilized to anticipate or fix sicknesses and wounds, for The theme attracts for the Stem Cell 2021 is “Frontiers in example, Parkinson's illness, type 1 diabetes, coronary illness, Stem Cells & Turning Ideas into Reality”. spinal string damage, strong dystrophy, Alzheimer's malady, Welcoming all of you for our Stem Cell 2021 involves strokes, osteoarthritis, vision and hearing misfortune. extraordinary delight, warmth and passion. We anticipate all Immature microorganisms could likewise be utilized to of you sharing your knowledge and information, look into supplant or repair tissue harmed by ailment or damage. thoughts and to make a sprinkle with new upgrades at this 2- days occasion. This time we have introduced some contemporary and recently updated and advanced highlights of Life sciences in Stem Cell 2021. Stem Cell 2021 wish to bring all the medicinal science, chemical engineering & tissue regeneration professionals and scientists under material science fields for our Smart Materials Meeting to collaborate and share their insight and their most Cancer Stem Cells, Bio-Makers Of Cancer Stem Cells, Stem current research to the whole Material Science Community. Cell Biology & Advances, Advanced In Tissue Regeneration, Also this time, Our International Conference on Stem Cell Embryonic Stem Cell, Reprogramming In Stem Cell & will be aims to haven for Multinational organizations, Transplantation, Treatment Of Diseases By Stem Cell entrepreneurs across the globe, the researchers and Therapeutics, Stem Cell Banking, Novel Stem Cell Therapy, academicians. -
Resume of Kejin Hu
CURRIULUM VITAE Of Kejin Hu PERSONAL INFORMATION Name: Kejin HU Visa status: USA citizen Language(s): English, Chinese Home city: Vestavia Hills, AL, 35226 RANK/TITLE, Assistant Professor Department: Biochemistry and Molecular Genetics Division: UAB Stem Cell Institute Business Address: SHEL 705, 1825 University Boulevard, Birmingham, AL, 35294 Phone: 205-934-4700 (office); 205-876-8693 (home); 205-703-6688 (cell) Fax: 205-975-3335 MEMBERSHIP: ISSCR (international Society for Stem Cell Research), since 2011 Member of Genetics Society of America (GSA, since 2006) EDUCATIONS June, 1999 to May, 2003, PhD, in marine molecular biology at the Department of Zoology, The University of Hong Kong, Hong Kong, China. July, 1995-October, 1997, MPhil, in fungal biochemistry/microbiology in the Hong Kong Polytechnic University. September, 1981-July, 1985, BSc in botany/agronomy at The Central China (Huazhong) Agricultural University, Wuhan, China. TEACHING EXPERIENCE: Advanced Stem Cell/Regenerative medicine, GBSC 709, Since 2014 SCIENTIFIC ACTIVITIES Ad hoc reviewer for the following journals: 1) Stem Cells; 2) Stem Cells and Development; 3) Stem Cell International; 4) Human Immunology; 5) Molecular Biotechnology; 6) Comparative Biochemistry and Physiology; 7) Journal of Heredity; 8) Scientific Reports; 9) Cellular Reprogramming; 10) Cell and Tissue Research; 11) Science Bulletin; 12) Reproduction, Fertility and Development. 1 Grant Reviewer for 1) Medical Research Council (MRC) of the United Kingdom (remote review, 2015); 2) New York Stem Cell Science (panel meeting from 09/28-09/30, 2016); 3) UAB internal grants SCIENTIFIC/ACADEMIC EXPERIENCE 2011 to present, Assistant Professor, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL September, 2007 to July, 2011, Research Associate in human iPSC reprogramming and human pluripotent stem cell biology, and their differentiation into blood lineage, Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI. -
New Doors to Open…And So Many! | Journal of Cell Science
New doors to open…and so many! | Journal of Cell Science Advertisement California Institute of Technology Log in Advanced search Home Articles About us For authors Journal info Contacts EDITORIAL New doors to open…and so many! Previous Article Next Article D.M. Glover Journal of Cell Science 2000 113: 359-360; This Issue Article Info & metrics Email Summary Share The pursuit of science is a wonderful journey of Citation Tools discovery along which there are a myriad of avenues to Alerts be explored. There have always been so many objects of fascination, so many questions to ask along the way, © Request Permissions We use cookies to help us improve this website. Learn more so many possibilities to understand new principles, that making the decision about which problem to address Article navigation and then having the self-discipline to explore it in depth Top challenge all who practice the art. How then are we, as Article cell biologists, to cope with the mountain of information Info & metrics that is accumulating as we enter the twenty-first https://jcs.biologists.org/content/113/3/359.long[8/10/2020 3:19:01 PM] New doors to open…and so many! | Journal of Cell Science century? We now have the potential to decipher the primary sequences of every single cellular protein for Related articles several model organisms. Just how are we to put this Web of Science PubMed information into an intelligible framework for Google Scholar understanding cell physiology? The turn of a century is a time at which we can permit ourselves the luxury of Cited by.. -
New Editor on Journal of Cell Science Sharon A
© 2017. Published by The Company of Biologists Ltd | Journal of Cell Science (2017) 130, 303 doi:10.1242/jcs.200345 EDITORIAL New Editor on Journal of Cell Science Sharon A. Ahmad (Executive Editor) and Michael Way (Editor-in-Chief) We are delighted to announce the appointment of Guangshuo Ou as an Editor on Journal of Cell Science. Guangshuo obtained his PhD in cell and developmental biology from the University of California, Davis. His thesis work, conducted under the guidance of Dr Jon Scholey, focused on ciliogenesis in Caenorhabditis elegans neurons and elucidated how microtubule-based motor proteins are used to build neuronal cilia. Guangshuo received his postdoctoral training with Dr Ron Vale at the University of California, San Francisco, where he developed imaging techniques to study neuroblast migration and division in C. elegans larvae and discovered a new myosin-based mechanism underlying neuroblast asymmetric division. In 2011 he was recruited by the Chinese government with the Junior One Thousand Talent Plan Award as an investigator at the Institute of Biophysics Chinese Academy of Sciences. In 2013 his group relocated to the School of Life Sciences at Tsinghua University in Beijing, and he became a principal investigator of the Joint Center for Life Sciences at Tsinghua and Peking Universities. He continues to study neuroblast development using C. elegans as a model organism. Guangshuo has had a long-standing interest in understanding the molecular and cellular mechanisms of cell division and cell migration in developmental systems. Guangshuo has developed imaging methods to follow neuroblast development in a living nematode larva, and his laboratory devised the somatic CRISPR– Cas9 technique to generate conditional knockouts in order to dissect the underlying molecular regulation. -
S. John Calise Department of Oral Biology [email protected] Graduate Program in Biomedical Sciences
Contact: University of Florida Health Science Center NSF Graduate Research Fellow P.O. Box 100424 Doctoral Candidate Gainesville, FL 32610-0424 Laboratory of Edward K.L. Chan, PhD 352-273-8851 (lab) S. John Calise Department of Oral Biology [email protected] Graduate Program in Biomedical Sciences EDUCATION University of Florida Ph.D. in Medical Sciences (in progress) Aug 2015 – present Gainesville, FL Graduate Program in Biomedical Sciences Concentration: Molecular Cell Biology Mentor: Edward K.L. Chan, PhD Awarded NSF Graduate Research Fellowship University of Florida B.S. in Business Administration Aug 2007 – May 2011 Gainesville, FL Major: Finance Graduated with Honors PREVIOUS RESEARCH POSITIONS University of Florida Laboratory Manager / Technician Sept 2011 – Aug 2015 Gainesville, FL Laboratory of Edward K.L. Chan, PhD Department of Oral Biology HONORS AND AWARDS (GRADUATE AND PROFESSIONAL) 2013, 1st Place, Dresden Prize for the Study of Autoantibodies, 11th Dresden Symposium on Autoantibodies 2014, 2nd Place, Oral & Poster Communication, 12th International Workshop on Autoantibodies and Autoimmunity 2015, University of Florida Graduate School Grinter Fellowship Award (stipend supplement for 3 years) 2015, American Society for Cell Biology Graduate Student Travel Award 2016, Pre-doctoral fellowship under Department of Oral Biology NIH/NIDCR Training Grant 2T90DE021990-06 2017, American Society for Cell Biology Early Career Meeting Grant 2017, Nominated for Associate Membership, Sigma Xi Scientific Research Honor Society 2017, National -
Autophagy in the Endocrine Glands
A WECKMAN and others Autophagy in the endocrine 52:2 R151–R163 Review glands Autophagy in the endocrine glands Andrea Weckman, Antonio Di Ieva, Fabio Rotondo1, Luis V Syro2, Leon D Ortiz3, Kalman Kovacs1 and Michael D Cusimano Division of Neurosurgery, Department of Surgery, St Michael’s Hospital, University of Toronto, Toronto, Ontario, Canada Correspondence 1Division of Pathology, Department of Laboratory Medicine, St Michael’s Hospital, University of Toronto, Toronto, should be addressed Ontario, Canada to A Di Ieva 2Department of Neurosurgery, Hospital Pablo Tobon Uribe and Clinica Medellin, Medellin, Colombia Email 3Division of Neurooncology, Instituto de Cancerologia, Clinic Las Americas, Medellin, Colombia [email protected] Abstract Autophagy is an important cellular process involving the degradation of intracellular Key Words components. Its regulation is complex and while there are many methods available, there is " autophagy currently no single effective way of detecting and monitoring autophagy. It has several " endocrine glands cellular functions that are conserved throughout the body, as well as a variety of different " crinophagy physiological roles depending on the context of its occurrence in the body. Autophagy is also " endocrine diseases involved in the pathology of a wide range of diseases. Within the endocrine system, autophagy has both its traditional conserved functions and specific functions. In the endocrine glands, autophagy plays a critical role in controlling intracellular hormone levels. In peptide-secreting cells of glands such as the pituitary gland, crinophagy, a specific form of autophagy, targets the secretory granules to control the levels of stored hormone. In steroid-secreting cells of glands such as the testes and adrenal gland, autophagy targets the steroid-producing organelles. -
Mechanical Roles of Vinculin/Β-Catenin Interaction in Adherens
bioRxiv preprint doi: https://doi.org/10.1101/770735; this version posted September 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 1 Mechanical Roles of Vinculin/β-catenin interaction in Adherens Junction 2 3 Cristina Bertocchi1, Andrea Ravasio1, Hui Ting Ong2, Yusuke Toyama2,3, Pakorn Kanchanawong2,4 4 1 5 Pontificia Universidad Católica de Chile, Santiago Chile, 8330025. 6 2 Mechanobiology Institute, Singapore. National University of Singapore, Republic of Singapore, 7 117411. 8 3Department of Biological Sciences, National University of Singapore, Singapore, Republic of 9 Singapore, 117543. 10 4Department of Biomedical Engineering. National University of Singapore, Republic of Singapore, 11 117411. 12 13 Correspondence: [email protected] or [email protected] 14 15 Summary 16 Mechanical force transmission through the adherens junctions (AJs) are highly regulated 17 processes essential for multicellular organization of tissues. AJ proteins such as E-cadherin, α- 18 catenin, and vinculin have been shown to be sensing or bearing mechanical forces being transmitted 19 between the actin cytoskeleton and the intercellular contacts. However, the molecular organization 20 and connectivity of these components remains not well understood. Using a super-resolution 21 microscopy approach, we report that vinculin, once activated, could form a direct structural 22 connection with β-catenin, which can bypass α-catenin, one of the main mechanotransducers in AJs. 23 Direct vinculin/β-catenin interaction is capable of supporting mechanical tension and contributes to 24 the stabilization of the cadherin-catenin complexes. -
'Microscopy' of Cellular Membranes Ingólfsson, Helgi I.; Arnarez, Clément; Periole, Xavier; Marrink, Siewert J
University of Groningen Computational 'microscopy' of cellular membranes Ingólfsson, Helgi I.; Arnarez, Clément; Periole, Xavier; Marrink, Siewert J. Published in: Journal of Cell Science DOI: 10.1242/jcs.176040 IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2016 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Ingólfsson, H. I., Arnarez, C., Periole, X., & Marrink, S. J. (2016). Computational 'microscopy' of cellular membranes. Journal of Cell Science, 129(2), 257-268. https://doi.org/10.1242/jcs.176040 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 12-11-2019 © 2016. Published by The Company of Biologists Ltd | Journal of Cell Science (2016) 129, 257-268 doi:10.1242/jcs.176040 COMMENTARY ARTICLE SERIES: IMAGING Computational ‘microscopy’ of cellular membranes Helgi I. -
Targeting Macrophages As a Candidate for Tissue Regeneration
Macrophages and Tissue Regeneration Zhang et al. Curr. Issues Mol. Biol. (2018) 29: 37-48. caister.com/cimb Snapshot: Targeting Macrophages as a Candidate for Tissue Regeneration Jing Zhang1,2,3, Yang Yang4, Zhi Yang4,5, Tian Li4,5 and Fulin Chen1,2,3* Introduction Macrophages, also known as "big eaters" due to 1Lab of Tissue Engineering, College of Life their phagocytic capacity, were first annotated by Sciences, Northwest University, 229 Taibai North Mechnikov in the late 18th century (Fraga et al., Road, Xi'an, 710069, P. R. China 2017; Tauber, 2003; Wan et al., 2017). They are a 2Provincial Key Laboratory of Biotechnology of specific mononuclear cell group abundant in almost Shaanxi, 229 Taibai North Road, Xi'an, 710069, P. every organ of higher animals. In mammals, R. China macrophages can derive from the yolk sac, fetal 3Key Laboratory of Resource Biology and liver, and bone marrow (Geissmann et al., 2010; Biotechnology in Western China Ministry of Wynn et al., 2013). Their differentiation is Education, 229 Taibai North Road, Xi'an, 710069, P. orchestrated by multiple growth factors (GFs) R. China. (Gordon, 2003; Sica et al., 2012). Tissue-resident 4College of Life Sciences, Northwest University, 229 macrophages, usually originating from the bone Taibai North Road, Xi'an, 710069, P. R. China marrow hematopoietic progenitors, has the ability of 5Department of Biomedical Engineering, The Fourth self-renewing (Geissmann et al., 2010; Jenkins et Military Medical University, 169 Changle West Road, al., 2011; Schulz et al., 2012; Davies et al., 2013). Xi'an 710032, China Generally, they are divided into two phenotypes, classically activated M1 macrophages and * Correspondence: [email protected] alternatively activated M2 macrophages, which refer to the state activated by Th1 and Th2 lymphocytes DOI: https://dx.doi.org/10.21775/cimb.029.037 or with interferon-gamma (INF-γ) and interleukin 4 (IL-4), respectively (Goerdt et al., 1999; Gordon, Abstract 2003; Sica et al., 2012).