Melanocytes: from Morphology to Application

Total Page:16

File Type:pdf, Size:1020Kb

Melanocytes: from Morphology to Application Skin Pharmacol Physiol 2009;22:114–121 Published online: February 4, 2009 DOI: 10.1159/000178870 Melanocytes: From Morphology to Application a a a, b a A. Santiago-Walker L. Li N.K. Haass M. Herlyn a b Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pa. , USA; Immune Imaging Program, Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, Sydney, N.S.W. , Australia Key Words skin is organized into two layers: the dermis, comprised M e l a n o c y t e ؒ K e r a t i n o c y t e ؒ M e l a n o m a ؒ Model ؒ S k i n ؒ of fibroblasts and endothelial cells, provides structure Three-dimensional reconstruct ؒ Drug and strength; and the epidermis, containing basal layer melanocytes, Langerhans cells and stratified layers of ke- ratinocytes which form the protective barrier between Abstract skin and the environment. The epidermis and dermis are Melanocytes in human skin are intricately regulated by kera- separated by the basement membrane which is formed by tinocytes and the surrounding stroma. The development of protein secretions of fibroblasts and keratinocytes. Each melanoma is thought to arise from disrupted melanocyte ho- melanocyte, through dendritic projections, interacts meostasis. It is now known that microenvironment plays a with approximately 36 keratinocytes in what is termed major role in maintenance of cellular homeostasis and can the epidermal melanin unit [1] . The control of melano- contribute to tumor initiation and tumor progression. Histor- cyte homeostasis and the process of melanoma develop- ically, melanocyte studies have been performed in two-di- ment are areas of active research. Here we highlight the mensional culture systems, and often with melanocytes cul- importance of using three-dimensional/organotypic cul- tured in the absence of keratinocytes. Here we present the tures in these studies. biological basis for the use of organotypic, three-dimension- al model systems in the study of melanoma, and highlight the features of the most utilized organotypic model sytems. Melanocyte Homeostasis and Regulation by Copyright © 2009 S. Karger AG, Basel Keratinocytes The proliferating cells of the skin are found primarily Introduction at the basal layer of the epidermis, where the melanocyte to keratinocyte ratio is approximately 1: 5. During child- Melanocytes are specialized, pigment-producing cells hood, melanocytes proliferate as needed during growth derived from the neural crest. Melanocytes respond to to maintain proper skin architecture. In adults, however, ultraviolet (UV) radiation by synthesizing melanin which melanocytes are for the most part quiescent and rarely forms a photoprotective cap over the nucleus of keratino- proliferate, though they maintain the capacity to do so. cytes, protecting skin cells from DNA damage. Human Melanocyte proliferation is primarily under the control © 2009 S. Karger AG, Basel Meenhard Herlyn, MD 1660–5527/09/0222–0114$26.00/0 The Wistar Institute Fax +41 61 306 12 34 3601 Spruce Street, Room 489 E-Mail [email protected] Accessible online at: Philadelphia, PA 19104 (USA) www.karger.com www.karger.com/spp Tel. +1 215 898 3950, Fax +1 215 898 0980, E-Mail [email protected] Keratinocyte-derived DecouplingDivision Migration Recoupling factors SCF, ET-1, GM-CSF E-cadherin Proliferation ACTH, ␣-MSH Melanogenesis Melanogenesis Color version available online E-cadherin SCF CCN3 E-cadherin Desmoglein 1 bFGF DDR1 ET-1 ET-3 HGF CCN3 Fig. 1. Dynamics of melanocyte proliferation in human skin. De- Melanocyte coupling: secretion of endothelin-1 (ET-1) by keratinocytes and DDR1 hepatocyte growth factor/scatter factor (HGF) by fibroblasts re- Integrin sults in the downregulation of E-cadherin expression. Division: bFGF, SCF, HGF Basement Collagen proliferation is induced by secreted factors including stem cell Laminin factor (SCF) secreted by keratinocytes and fibroblasts, basic fibro- membrane blast growth factor (bFGF) by keratinocytes and ET-3 by fibro- blasts. Migration: expression of the matricellular protein CCN3 Fibroblast-derived and its receptor discoidin domain receptor 1 (DDR1) is required factors for proper melanocyte localization on the basement membrane. Recoupling: expression of E-cadherin is increased, recoupling to keratinocytes and dendrite extension occurs. Fig. 2. Melanocyte microenvironment. Keratinocytes secrete fac- tors that promote melanocyte proliferation and melanogenesis including stem cell factor (SCF), endothelin-1 (ET-1), granulo- cyte-macrophage colony-stimulating factor (GM-CSF), adreno- corticotrophic hormone (ACTH) and ␣ -melanocyte stimulating ␣ of keratinocytes. Cell-cell contacts with keratinocytes are hormone ( -MSH). Fibroblasts also contribute to melanocyte regulation by secretion of basic fibroblast growth factor (bFGF), known to be important for the maintenance of melano- SCF and hepatocyte growth factor (HGF). Melanocytes maintain cyte homeostasis. In order for a melanocyte to proliferate, proper localization by binding to collagen and laminin on the the following steps are proposed ( fig. 1 ): (1) retraction of basement membrane. Fukunaga-Kalabis et al. [9, 10] recently re- dendrites and decoupling of melanocytes from surround- ported a role for CCN3 and discoidin domain receptor 1 (DDR1) ing keratinocytes and the basement membrane; (2) pro- in melanocyte basement membrane localization. liferation; (3) repositioning via migration along the base- ment membrane; (4) dendrite extension and recoupling to keratinocytes [2] . Keratinocytes signal to melanocytes to control proliferation via cell-cell contacts mediated by undergo an E- to N-cadherin class switch, allowing me- cadherins as well as by the secretion of signaling mole- lanocytes to escape keratinocyte control as well as pro- cules and growth factors ( fig. 2 ). moting binding to dermal fibroblasts and endothelial The cadherins are a family of cell surface glycopro- cells. Not only are melanoma cells free of keratinocyte teins which mediate calcium-dependent cell adhesion, control over proliferation under these conditions, but in- and represent the transmembrane components of cell- teractions with fibroblasts and endothelial cells allow cell adhesion junctions. They are homophilic binding melanoma cells to invade and survive in the dermis [5] . proteins allowing cells of the same type, or same cad- N-cadherin induces melanoma cell migration, and pro- herin expression pattern, to bind and organize into tis- motes survival via activation of Akt, increased ␤ -catenin sues [3] . In skin, cadherin expression is cell type specific expression and inactivation of the proapoptotic protein and critical for maintenance of skin architecture and reg- Bad [6] . The importance of this cadherin class switch is ulation of homeostasis. E-cadherin is expressed on mela- supported by experiments showing that re-expression of nocytes, keratinocytes and Langerhans cells of the epi- E-cadherin in human melanoma cells restored keratino- dermis, while N-cadherin is expressed on dermal fibro- cyte coupling and inhibited invasive potential [7] . blasts and endothelial cells [4] . This differential cadherin Integrin-mediated attachment also plays a role in me- expression is exploited in melanoma, where melanocytes lanocyte maintenance. Integrins are cell surface recep- Melanocytes: From Morphology to Skin Pharmacol Physiol 2009;22:114–121 115 Application tors for extracellular matrix (ECM) proteins such as lam- hesion molecule (Mel-CAM/MUC18) and ␤ 3 integrin inin and collagen. Integrins, which are comprised of het- [13] . Melanocyte phenotype can be rescued by the addi- erodimers of ␣ - and ␤ -subunits, connect the ECM to the tion of keratinocytes to melanocyte cultures. Keratino- actin cytoskeleton and are required for melanocyte at- cytes regain control over melanocyte proliferation such tachment to and release from the basement membrane that when keratinocytes and melanocytes are seeded at during migration. In addition to their role in attachment, fixed ratios, these ratios are maintained as the cells pro- integrins impact multiple aspects of cell physiology in- liferate. The multidendritic morphology of melanocytes cluding proliferation, differentiation and survival. Al- is restored upon coculture. Most interestingly, expression tered integrin expression is thought to help melanocytes of melanoma-associated antigens is lost within 3–4 days survive in the dermis, specifically integrins ␣v ␤3 and of coculture [14] . In support of this, in vivo nevocytes ␣5 ␤1 are indicative of melanoma progression [8] . (nests or groupings of melanocytes) residing in the der- Recently, work from our laboratory has revealed a role mis, separated from keratinocytes, express melanoma- for the matricellular protein CCN3 in melanocyte ho- associated antigens while nevocytes localized in the epi- meostasis. CCN3 (nephroblastoma overexpressed) was dermis do not. Coculture of dermal nevocytes with kera- found to be upregulated in melanocytes and secreted in tinocytes can restore normal melanocytic phenotype response to coculture with keratinocytes. CCN3 expres- [13] . Further evidence for the role of keratinocytes on me- sion inhibited melanocyte proliferation and was required lanocyte homeostatis comes from a recently developed for proper melanocyte localization on the basement mouse model in which the retinoid X receptor ␣ (RXR ␣ ) membrane. CCN3, via the receptor tyrosine kinase dis- was knocked out selectively in keratinocytes. Keratino- coidin domain receptor 1 (DDR1), promotes
Recommended publications
  • Assessment of Melanocyte-Specific Primary and Memory Autoimmune Responses in Vitiligo- Prone Smyth and Vitiligo-Susceptible, Non-Expressing Brown Line Chickens
    University of Arkansas, Fayetteville ScholarWorks@UARK Theses and Dissertations 8-2018 Assessment of Melanocyte-Specific rP imary and Memory Autoimmune Responses in Vitiligo-Prone Smyth and Vitiligo-Susceptible, Non-Expressing Brown Line Chickens Daniel Morales Falcon University of Arkansas, Fayetteville Follow this and additional works at: https://scholarworks.uark.edu/etd Part of the Cell Biology Commons, and the Immunology of Infectious Disease Commons Recommended Citation Falcon, Daniel Morales, "Assessment of Melanocyte-Specific rP imary and Memory Autoimmune Responses in Vitiligo-Prone Smyth and Vitiligo-Susceptible, Non-Expressing Brown Line Chickens" (2018). Theses and Dissertations. 2912. https://scholarworks.uark.edu/etd/2912 This Dissertation is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. Assessment of Melanocyte-Specific Primary and Memory Autoimmune Responses in Vitiligo- Prone Smyth and Vitiligo-Susceptible, Non-Expressing Brown Line Chickens A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Cell and Molecular Biology by Daniel Morales Falcon University of California, Riverside Bachelor of Science in Biology, 2003 August 2018 University of Arkansas This dissertation is approved for recommendation to the Graduate Council. ____________________________________ Gisela F. Erf, Ph.D. Dissertation Director ____________________________________ ___________________________________ Yuchun Du, Ph.D. David McNabb, Ph.D. Committee Member Committee Member ____________________________________ Suresh Thallapuranam, Ph.D. Committee Member Abstract Vitiligo is an acquired de-pigmentation disorder characterized by the post-natal loss of epidermal melanocytes (pigment-producing cells) resulting in the appearance of white patches in the skin.
    [Show full text]
  • Autoimmune Melanocyte Destruction Is Required for Robust CD8+ Memory T Cell Responses to Mouse Melanoma Katelyn T
    Research article Autoimmune melanocyte destruction is required for robust CD8+ memory T cell responses to mouse melanoma Katelyn T. Byrne,1 Anik L. Côté,1 Peisheng Zhang,1 Shannon M. Steinberg,1 Yanxia Guo,1 Rameeza Allie,1 Weijun Zhang,1 Marc S. Ernstoff,2 Edward J. Usherwood,1 and Mary Jo Turk1,3 1Department of Microbiology and Immunology, Dartmouth Medical School, 2Section of Hematology/Oncology, Department of Medicine, Dartmouth Hitchcock Medical Center, and 3The Norris Cotton Cancer Center, Lebanon, New Hampshire, USA. A link between autoimmunity and improved antitumor immunity has long been recognized, although the exact mechanistic relationship between these two phenomena remains unclear. In the present study we have found that vitiligo, the autoimmune destruction of melanocytes, generates self antigen required for mounting persistent and protective memory CD8+ T cell responses to melanoma. Vitiligo developed in approximately 60% of mice that were depleted of regulatory CD4+ T cells and then subjected to surgical excision of large established B16 melanomas. Mice with vitiligo generated 10-fold larger populations of CD8+ memory T cells specific for shared melanoma/melanocyte antigens. CD8+ T cells in mice with vitiligo acquired phenotypic and functional characteristics of effector memory, suggesting that they were supported by ongoing antigen stimulation. Such responses were not generated in melanocyte-deficient mice, indicating a requirement for melanocyte destruction in maintaining CD8+ T cell immunity to melanoma. Vitiligo-associated memory CD8+ T cells provided durable tumor protection, were capable of mounting a rapid recall response to melanoma, and did not demonstrate phenotypic or functional signs of exhaustion even after many months of exposure to antigen.
    [Show full text]
  • Dermal Fibroblasts Internalize Phosphatidylserine-Exposed Secretory Melanosome Clusters and Apoptotic Melanocytes
    International Journal of Molecular Sciences Article Dermal Fibroblasts Internalize Phosphatidylserine-Exposed Secretory Melanosome Clusters and Apoptotic Melanocytes Hideya Ando 1,*, Satoshi Yoshimoto 1, Moemi Yoshida 1, Nene Shimoda 1, Ryosuke Tadokoro 1, Haruka Kohda 2, Mami Ishikawa 2, Takahito Nishikata 2, Bunpei Katayama 3, Toshiyuki Ozawa 3, Daisuke Tsuruta 3 , Ken-ichi Mizutani 4, Masayuki Yagi 5 and Masamitsu Ichihashi 4,6,7 1 Department of Applied Chemistry and Biotechnology, Okayama University of Science, Okayama 700-0005, Japan; [email protected] (S.Y.); [email protected] (M.Y.); [email protected] (N.S.); [email protected] (R.T.) 2 Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan; [email protected] (H.K.); [email protected] (M.I.); [email protected] (T.N.) 3 Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan; [email protected] (B.K.); [email protected] (T.O.); [email protected] (D.T.) 4 Laboratory of Stem Cell Biology, Graduate School of Pharmaceutical Sciences, Kobe Gakuin University, Kobe 650-8586, Japan; [email protected] (K.M.); [email protected] (M.I.) 5 Rosette Co., Tokyo 140-0004, Japan; [email protected] 6 Anti-Aging Medical Research Center, Doshisha University, Kyoto 610-0394, Japan 7 Arts Ginza Clinic, Tokyo 105-0004, Japan * Correspondence: [email protected]; Tel.: +81-86-256-9726 Received: 28 May 2020; Accepted: 9 August 2020; Published: 12 August 2020 Abstract: Pigmentation in the dermis is known to be caused by melanophages, defined as melanosome-laden macrophages.
    [Show full text]
  • Nomina Histologica Veterinaria, First Edition
    NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria.
    [Show full text]
  • Diccionario De Siglas Médicas Y Otras Abreviaturas, Epónimos Y Términos Médicos Relacionados Con La Codificación De Las Altas Hospitalarias
    Diccionario de siglas médicas y otras abreviaturas, epónimos y términos médicos relacionados con la codificación de las altas hospitalarias JAVIER YETANO LAGUNA VICENT ALBEROLA CUÑAT COORDINACION EDITORIAL: Agustín RIVERO CUADRADO Rogelio COZAR RUIZ REALIZADO POR: Javier YETANO LAGUNA Vicent ALBEROLA CUÑAT MIEMBROS PERMANENTES DEL COMITÉ EDITORIAL: Jesús TRANCOSO ESTRADA M.ª Dolores del PINO JIMENEZ Paloma FERNANDEZ MUÑOZ Joan Ferrer Riera M.ª Coromoto RODRIGUEZ DEL ROSARIO Paz RODRIGUEZ CUNDIN Fernando ROJO ROLDAN Carmen VILCHEZ PERDIGON Abel FERNANDEZ SIERRA M.ª Antonia VÁREZ PASTRANA Belén BENEITEZ MORALEJO Guillermo RODRIGUEZ MARTINEZ Ana VARA LORENZO Carmen SALIDO CAMPOS Arturo ROMERO GUTIERREZ Isabel DE LA RIVA JIMENEZ Pilar MORI VARA M.ª Gala GUTIERREZ MIRAS L. Javier LIZARRAGA DALLO Yolanda MONTES GARCIA M.ª Isabel MENDIBURU PEREZ Vicent ALBEROLA CUÑAT Adolfo CESTAFE MARTINEZ MIEMBROS ASESORES DEL COMITÉ EDITORIAL: Pedro MOLINA COLL M.ª Teresa DE PEDRO Montserrat LOPEZ HEREDERO Jovita PRINTZ Soledad SAÑUDO GARCIA M.ª Luisa TAMAYO CANILLAS Román GARCIA DE LA INFANTA José DEL RIO MATA Pilar RODRIGUEZ MANZANO Esther VILA RIBAS Elena ESTEBAN BAEZ José Alfonso DELGADO Irene ABAD PEREZ José M.ª JUANCO VAZQUEZ Teresa SOLER ROS José Ramón MENDEZ MONTESINO Javier YETANO LAGUNA Margarita LLORIA BERNACER Eloísa CASADO FERNANDEZ M.ª Mar SENDINO GARCIA Fernando PEÑA RUIZ Eduard GUASP SITJAR SECRETARIA: Esther GRANDE LOPEZ Edita y distribuye: © MINISTERIO DE SANIDAD Y CONSUMO CENTRO DE PUBLICACIONES Paseo del Prado, 18-20 - 28014 Madrid ISBN:
    [Show full text]
  • Exosomes Released by Keratinocytes Modulate Melanocyte Pigmentation
    ARTICLE Received 18 Dec 2014 | Accepted 15 May 2015 | Published 24 Jun 2015 DOI: 10.1038/ncomms8506 OPEN Exosomes released by keratinocytes modulate melanocyte pigmentation Alessandra Lo Cicero1,2,3,Ce´dric Delevoye1,2, Floriane Gilles-Marsens1,2, Damarys Loew4, Florent Dingli4, Christelle Gue´re´5, Nathalie Andre´5, Katell Vie´5, Guillaume van Niel1,2,3 &Grac¸a Raposo1,2,3 Cells secrete extracellular vesicles (EVs), exosomes and microvesicles, which transfer proteins, lipids and RNAs to regulate recipient cell functions. Skin pigmentation relies on a tight dialogue between keratinocytes and melanocytes in the epidermis. Here we report that exosomes secreted by keratinocytes enhance melanin synthesis by increasing both the expression and activity of melanosomal proteins. Furthermore, we show that the function of keratinocyte-derived exosomes is phototype-dependent and is modulated by ultraviolet B. In sum, this study uncovers an important physiological function for exosomes in human pigmentation and opens new avenues in our understanding of how pigmentation is regulated by intercellular communication in both healthy and diseased states. 1 Institut Curie, PSL Research University, UMR144, CNRS, F-75248 Paris, France. 2 Structure and Membrane Compartments, Centre National de la Recherche Scientifique, UMR144, Paris F-75248, France. 3 Cell and Tissue Imaging Facility, Infrastructures en Biologie Sante´ et Agronomie (IBiSA), Paris F-75248, France. 4 Institut Curie, Centre de Recherche, Laboratoire de Spectrome´trie de Masse Prote´omique, Paris F-75248, France. 5 Laboratoires Clarins—31 chausse´e Jules Ce´sar, Pontoise 95300, France. Correspondence and requests for materials should be addressed to G.R. (email: [email protected]).
    [Show full text]
  • On the Role of Melanoma-Specific CD8 T-Cell Immunity in Disease
    4754 Vol. 10, 4754–4760, July 15, 2004 Clinical Cancer Research On the Role of Melanoma-Specific CD8؉ T-Cell Immunity in Disease Progression of Advanced-Stage Melanoma Patients Monique van Oijen,1 Adriaan Bins,1 cellular immune response, because the presence of tumor-infil- Sjoerd Elias,2 Johan Sein,2 Pauline Weder,1 trating T lymphocytes in primary melanoma and in melanoma Gijsbert de Gast,2 Henk Mallo,1 Maarten Gallee,3 lymph node metastases are independent positive prognostic fac- 4 1 tors (5, 6). Harm van Tinteren, Ton Schumacher, and In past years, extensive efforts to identify target molecules 1,2 John Haanen for melanoma-reactive T cells have resulted in the identification Divisions of 1Immunology, 2Medical Oncology, 3Oncologic of a large set of melanoma-associated antigens (7). These anti- 4 Diagnostics, and Statistics, The Netherlands Cancer Institute, gens can be classified as melanocyte lineage-specific antigens Amsterdam, the Netherlands (MART-1/Melan-A, tyrosinase, gp100) and antigens derived from genes expressed in testis and a variety of cancers (includ- ABSTRACT ing MAGE-family, NY-ESO-1, PRAME). Melanocyte lineage Cytotoxic T-cell immunity directed against melanoso- antigens are expressed in a large fraction of melanomas, and a mal differentiation antigens is arguably the best-studied and substantial number of epitopes from these antigens that are most prevalent form of tumor-specific T-cell immunity in recognized by cytotoxic T cells have been mapped (8). With the humans. Despite this, the role of T-cell responses directed aid of soluble tetramerized MHC complexes containing these ϩ against melanosomal antigens in disease progression has not epitopes, melanosomal antigen-specific CD8 T cells have been been elucidated.
    [Show full text]
  • Melanocyte Differentiation and Epidermal Pigmentation Is Regulated by Polarity Proteins
    bioRxiv preprint doi: https://doi.org/10.1101/2020.04.20.051722; this version posted April 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Melanocyte differentiation and epidermal pigmentation is regulated by polarity proteins Sina K. Knapp1,2 and Sandra Iden1,2,3* 1Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Germany 2Center for Molecular Medicine Cologne (CMMC), University of Cologne, Germany 3Cell and Developmental Biology, Saarland University, Faculty of Medicine, Homburg/Saar, Germany *correspondence: [email protected], Cell and Developmental Biology, Saarland University, Faculty of Medicine, Kirrberger Str., Building 61.4, 66421 Homburg/Saar, Germany ABSTRACT Pigmentation serves various purposes such as protection, camouflage, or attraction. In the skin epidermis, melanocytes react to certain environmental signals with melanin production and release, thereby ensuring photo-protection. For this, melanocytes acquire a highly polarized and dendritic architecture that facilitates interactions with surrounding keratinocytes and melanin transfer. How the morphology and function of these neural crest-derived cells is regulated remains poorly understood. Here, using mouse genetics and primary cell cultures, we show that conserved proteins of the mammalian Par3-aPKC polarity complex are required for epidermal pigmentation. Melanocyte-specific deletion of Par3 in mice caused skin hypopigmentation, reduced expression of components of the melanin synthesis pathway, and altered dendritic morphology. Mechanistically, Par3 was necessary downstream of -melanocyte stimulating hormone (-MSH) to elicit melanin production.
    [Show full text]
  • Melanocyte-Keratinocyte Interactions in Vivo: the Fate of Melanosomes
    YALE JOURNAL OF BIOLOGY AND MEDICINE 46, 384-396 (1973) Melanocyte-Keratinocyte Interactions in Vivo: The Fate of Melanosomes. K. WOLFF Department of Dermatology I, University of Vienna, Aiustria Studies on pigment donation in tissue culture (1-5) indicate that melanosome transfer is a cytophagic process during which a portion of a melanocyte dendrite is pinched off by the epidermal cell so that melanosomes and melanocyte cytoplasm are incorporated into the keratinocyte. At the ultrastructural level, one would ex- rect to see, at this stage, a cluster of melanosomes embedded in a cytoplasmic matrix surrounded by two membranes: one derived from the melanocyte and one belonging to the epidermal cell (Fig. 1). Although such images have not been pub- lished in reports on the electron microscopy of tissue culture (which readily reveals "cytophagocytosis"-phenomena at the light microscope level) they have been ob- served in in vivo specimens of hair bulbs (6), developing fowl feathers (7), and epidermis (Fig. 1). The melanosome complex, however, as it usually appears within keratinocytcs, is limited by only one membrane. It has been suggested (6, 7) that the inner (the melanocytic) membrane is rapidly decomposed but since double membrane-delimited structures occur only in the cell periphery they could equally well represent cross-sectioned dendrites of melanocytes bulging into the cytoplasm of the epidermal cell (Fig. 1). Also, if the matrix of a melanosome complex represents melanocyte cytoplasm it is surprising that it never contains identifiable cytoplasmic residues, such as mitochondria, microfilaments, or similar structures. In heterophagic and autophagic vacuoles such organelles may persist for a considerable time before they are decomposed (8, 9).
    [Show full text]
  • Human Pigmentation Variation: Evolution, Genetic Basis, and Implications for Public Health
    YEARBOOK OF PHYSICAL ANTHROPOLOGY 50:85–105 (2007) Human Pigmentation Variation: Evolution, Genetic Basis, and Implications for Public Health Esteban J. Parra* Department of Anthropology, University of Toronto at Mississauga, Mississauga, ON, Canada L5L 1C6 KEY WORDS pigmentation; evolutionary factors; genes; public health ABSTRACT Pigmentation, which is primarily deter- tic interpretations of human variation can be. It is erro- mined by the amount, the type, and the distribution of neous to extrapolate the patterns of variation observed melanin, shows a remarkable diversity in human popu- in superficial traits such as pigmentation to the rest of lations, and in this sense, it is an atypical trait. Numer- the genome. It is similarly misleading to suggest, based ous genetic studies have indicated that the average pro- on the ‘‘average’’ genomic picture, that variation among portion of genetic variation due to differences among human populations is irrelevant. The study of the genes major continental groups is just 10–15% of the total underlying human pigmentation diversity brings to the genetic variation. In contrast, skin pigmentation shows forefront the mosaic nature of human genetic variation: large differences among continental populations. The our genome is composed of a myriad of segments with reasons for this discrepancy can be traced back primarily different patterns of variation and evolutionary histories. to the strong influence of natural selection, which has 2) Pigmentation can be very useful to understand the shaped the distribution of pigmentation according to a genetic architecture of complex traits. The pigmentation latitudinal gradient. Research during the last 5 years of unexposed areas of the skin (constitutive pigmenta- has substantially increased our understanding of the tion) is relatively unaffected by environmental influences genes involved in normal pigmentation variation in during an individual’s lifetime when compared with human populations.
    [Show full text]
  • Melanin Transfer in the Epidermis: the Pursuit of Skin Pigmentation Control Mechanisms
    International Journal of Molecular Sciences Review Melanin Transfer in the Epidermis: The Pursuit of Skin Pigmentation Control Mechanisms Hugo Moreiras † , Miguel C. Seabra and Duarte C. Barral * iNOVA4Health, CEDOC, NOVA Medical School, NMS, Universidade NOVA de Lisboa, 1169-056 Lisboa, Portugal; [email protected] (H.M.); [email protected] (M.C.S.) * Correspondence: [email protected]; Tel.: +351-218-803-102 † Present address: The Charles Institute of Dermatology, School of Medicine, University College Dublin, D04 V1W8 Dublin, Ireland. Abstract: The mechanisms by which the pigment melanin is transferred from melanocytes and processed within keratinocytes to achieve skin pigmentation remain ill-characterized. Nevertheless, several models have emerged in the past decades to explain the transfer process. Here, we review the proposed models for melanin transfer in the skin epidermis, the available evidence supporting each one, and the recent observations in favor of the exo/phagocytosis and shed vesicles models. In order to reconcile the transfer models, we propose that different mechanisms could co-exist to sustain skin pigmentation under different conditions. We also discuss the limited knowledge about melanin processing within keratinocytes. Finally, we pinpoint new questions that ought to be addressed to solve the long-lasting quest for the understanding of how basal skin pigmentation is controlled. This knowledge will allow the emergence of new strategies to treat pigmentary disorders that cause a significant socio-economic burden to patients and healthcare systems worldwide and could also have relevant cosmetic applications. Citation: Moreiras, H.; Seabra, M.C.; Keywords: melanin; melanosome; melanocore; melanocyte; keratinocyte; skin pigmentation; Barral, D.C.
    [Show full text]
  • 26 April 2010 TE Prepublication Page 1 Nomina Generalia General Terms
    26 April 2010 TE PrePublication Page 1 Nomina generalia General terms E1.0.0.0.0.0.1 Modus reproductionis Reproductive mode E1.0.0.0.0.0.2 Reproductio sexualis Sexual reproduction E1.0.0.0.0.0.3 Viviparitas Viviparity E1.0.0.0.0.0.4 Heterogamia Heterogamy E1.0.0.0.0.0.5 Endogamia Endogamy E1.0.0.0.0.0.6 Sequentia reproductionis Reproductive sequence E1.0.0.0.0.0.7 Ovulatio Ovulation E1.0.0.0.0.0.8 Erectio Erection E1.0.0.0.0.0.9 Coitus Coitus; Sexual intercourse E1.0.0.0.0.0.10 Ejaculatio1 Ejaculation E1.0.0.0.0.0.11 Emissio Emission E1.0.0.0.0.0.12 Ejaculatio vera Ejaculation proper E1.0.0.0.0.0.13 Semen Semen; Ejaculate E1.0.0.0.0.0.14 Inseminatio Insemination E1.0.0.0.0.0.15 Fertilisatio Fertilization E1.0.0.0.0.0.16 Fecundatio Fecundation; Impregnation E1.0.0.0.0.0.17 Superfecundatio Superfecundation E1.0.0.0.0.0.18 Superimpregnatio Superimpregnation E1.0.0.0.0.0.19 Superfetatio Superfetation E1.0.0.0.0.0.20 Ontogenesis Ontogeny E1.0.0.0.0.0.21 Ontogenesis praenatalis Prenatal ontogeny E1.0.0.0.0.0.22 Tempus praenatale; Tempus gestationis Prenatal period; Gestation period E1.0.0.0.0.0.23 Vita praenatalis Prenatal life E1.0.0.0.0.0.24 Vita intrauterina Intra-uterine life E1.0.0.0.0.0.25 Embryogenesis2 Embryogenesis; Embryogeny E1.0.0.0.0.0.26 Fetogenesis3 Fetogenesis E1.0.0.0.0.0.27 Tempus natale Birth period E1.0.0.0.0.0.28 Ontogenesis postnatalis Postnatal ontogeny E1.0.0.0.0.0.29 Vita postnatalis Postnatal life E1.0.1.0.0.0.1 Mensurae embryonicae et fetales4 Embryonic and fetal measurements E1.0.1.0.0.0.2 Aetas a fecundatione5 Fertilization
    [Show full text]