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Apoptotic RBM38, TUSC2 and ANO1 Proteins ใน Human Serum Proteome มีความสัมพันธ์กับภาวะ

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Apoptotic RBM38, TUSC2 and ANO1 Proteins ใน Human Serum Proteome มีความสัมพันธ์กับภาวะ วารสารพิษวิทยาไทย 2561 ; 33(2) : 9-28 9 วารสารพิษวิทยาไทย Apoptotic RBM38, TUSC2 and ANO1 Proteins ใน Human Serum Proteome มีความสัมพันธ์กับภาวะ THAI JOURNAL OF TOXICOLOGY โฮโมซิสเตอนี สูงในเลอื ด Volume 33, Number 2, July-December 2018 ณฐั วุฒิ ลายน า้ เงิน1, สิทธิรักษ์ รอยตระกูล2, ตวงรัตน์ ต้ังเสถียรพนั ธ์ุ3, ปิยะมิตร ศรีธรา4, จินตนา ศิริวราศัย5* Research Articles 1หลักสูตรวิทยาศาสตรมหาบัณฑิต (โภชนศาสตร์) โครงการร่วมคณะแพทยศาสตร์โรงพยาบาลรามาธิบดีและสถาบัน Apoptotic RBM38, TUSC2 and ANO1 Proteins in Human Serum Proteome Are xx Associated with Hyperhomocysteinemia โภชนาการ มหาวิทยาลัยมหิดล 2 Nuttawut Lainumngen, Sittiruk Roytrakul, Tuangrat Tangstheanphan, Piyamitr Sritara, ห้องปฏิบัติการวิจัยโปรตีโอมิกส์ สถาบันจีโนม ศูนยพ์ นั ธุวศิ วกรรมและเทคโนโลยชี ีวภาพแห่งชาติ ปทุมธานี Jintana Sirivarasai ………….………………………………………………………………………… 3 ฝ่ายการแพทยแ์ ละอนามยั การไฟฟ้ าฝ่ายผลิตแห่งประเทศไทย นนทบุรี 4สาขาวิชาอายุรศาสตร์โรคหัวใจ ภาควิชาอายุรศาสตร์, 5กลุม่ สาขาวชิ าโภชนศาสตร์ คณะแพทยศาสตร์โรงพยาบาล Migration of Melamine from Melamine-formaldehyde Food Containers Available on xx The Market รามาธิบดี มหาวิทยาลัยมหิดล Uma Boriboon, Sasithorn Homdumrongwong, Passarin Saysuwary ……………….…………. บทคัดย่อ In vitro Antioxidant Activities of Nipa Palm Vinegar xx ภาวะโฮโมซิสเตอีนสูงในเลือด เป็นหน่ึงในปัจจยั เสี่ยงต่อการเกิดโรคหลอดเลือดหวั ใจ เนื่องจาก Udomratana Vattanasit, Supabhorn Yimthiang, Wiyada Kwanhian……………………………. สามารถชกั นา ใหเ้ กิดภาวะเซลลข์ องกลา้ มเน้ือหลอดเลือดตายได้ (apoptosis) ในปัจจุบันการศึกษาทางด้าน Effects of Alcoholic Extracts of Sweet-Fruited Tamarind (Tamarindus indica L.) and xx โปรติโอมิกส์มีการนา มาใชก้ นั อยา่ งแพร่หลายในงานวิจัยทางด้านวิทยาศาสตร์และทางคลินิก งานวจิ ยั น้ีเป็น Banana (Musa sapientum L.) on H2O2-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells การศึกษาเบ้ืองตน้ ที่ใชเ้ ทคนิคทางโปรติโอมิกส์ โดยมีวตั ถุประสงคเ์ พื่อศึกษาความแตกตา่ งของโปรตีนที่ Jiraporn Rangphueng, Ratchanee Kongkachuichai, Sujira Mukda, Jintana Sirivarasai, แสดงออกที่เกี่ยวขอ้ งกบั การเกิดโรคหลอดเลือดหวั ใจในผทู้ ี่มีภาวะโฮโมซิสเตอีนสูงในเลือด 3 ระดับ ใช้ Pattarana Sae-chew, Supanart Srisala, Bunyada Jithontham and Rodjana Chunhabundit chemiluminescense immunoassay สาหรับ วัดระดับโฮโมซิสเตอีนในเลือด ศึกษาการแสดงออกของโปรตีน ในซีรัมด้วยเทคนิค gel electrophoresis และ LC/MS-MS วิเคราะห์ปริมาณโปรตีนด้วย DecyderMs software Review Article เปรียบเทียบความแตกตา่ งของโปรตีนในรูปของ Venn diagram ด้วย Jvenn และจดั กลุ่มของโปรตีนด้วย Panther software ทา นายความสัมพนั ธ์ระหวา่ งโปรตีนและสารโมเลกุลอื่นโดย STITCH โปรแกรมเวอร์ชน่ั A Review of Thermal and pH Stability of Important Foodborne Pathogen Toxins and xx Their Applications 5.0 ผลการศึกษาพบวา่ มีโปรตีนเฉพาะ 8 ชนิดที่มีการพบในปริมาณสูง (up-regulated) ในกลุ่มคนที่มีภาวะ Woranon Leevongcharoen, Sasiphatlapa Thanpolphat, Tumnoon Charaslertrangsi ……….. โฮโมซิสเตอีนสูงในเลือด ไดแ้ ก่ MUC-19, LPAR1, MAPO2, GPALPP1, FECH, TUSC2, RBM38 และ Thirdhand Smoke : Emerging, Existing, and Never Ending xx ANO1 ประเด็นสา คญั พบวา่ RBM38, TUSC2 และ ANO1 เป็นโปรตีนที่พบเฉพาะในกลุ่มที่มีโฮโมซิสเตอีน Udomratana Vattanasit, Sawanya Laohaprapanon and Prasert Makkaew…………………… ในเลือดสูงกวา่ 50 µmol/L ที่มีสัมพนั ธ์กบั โรคหลอดเลือดซ่ึงเกี่ยวขอ้ งกบั ความผิดปกติในขบวนการตายของ เซลล์ ข้อมูลเชิงลึกที่ไดจ้ ากการศึกษาน้ีจะช่วยเพ่ิมเติมความรู้ความเข้าใจในการเปลี่ยนแปลงของโปรตีน ภายหลังการแปลงรหัสที่มีความสัมพนั ธ์กบั ความผิดปกติของระดับโฮโมซิสเตอีน และความเสี่ยงของการ เกิดโรคหลอดเลือดหวั ใจได้ ค าส าคัญ : ภาวะโฮโมซิสเตอีนสูงในเลือด โปรตีโอมิกส์ เซลล์ตาย (apoptosis) *ผู้รับผดิ ชอบบทความ ผศ.ดร.จินตนา ศิริวราศัย กลุ่มสาขาวิชาโภชนศาสตร์ คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี มหาวิทยาลัยมหิดล ถ. พระราม 6 กรุงเทพฯ 10400 E-mail: [email protected] วารสารพิษวิทยาไทย 2561 ; 33(2) : 9-28 10 Apoptotic RBM38, TUSC2 and ANO1 Proteins in Human Serum Proteome Are Introduction cardiovascular diseases. Notably, they Associated with Hyperhomocysteinemia highlighted the novel association among Proteomics is one of the new era of Nuttawut Lainumngen1, Sittiruk Roytrakul2, Tuangrat Tangstheanphan3, Piyamitr genetic factor, homocysteine levels and Sritara4, Jintana Sirivarasai5* omics studies involving whole protein alteration of protein expression. However, expressions profiles in cells together with 1Master of Science Program in Nutrition, Faculty of Medicine Ramathibodi Hospital and Institute of Nutrition, previous proteomic studies of hyper- Mahidol University, Thailand quantify and identify the protein product 2 Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), homocysteinemia in human biofluids of all genes. Proteomics is able to provide Pathum Thani, Thailand related homocysteine (e.g., serum, plasma, 3 Health Office, Electricity Generating Authority of Thailand, Nonthaburi, Thailand better understanding in molecular basis 4Department of Medicine 5Graduate Program in Nutrition, Faculty of Medicine Ramathibodi Hospital, Mahidol urine, and saliva) are very limited. To our insight of diseases and to identify University, Thailand knowledge, this is a first preliminary study biological marker, which may be a specific Abstract to investigate serum protein expression in indicator in different conditions. Previous Hyperhomocysteinemia (HHcy) is known as an independent risk factor for Thai men with hyperhomocysteinemia. studies of proteomics and homocysteine in cardiovascular diseases. It can induce apoptosis of vascular smooth muscle cells. Currently, Well-known risk factors of animal model reported some significant proteomic has been widely applied in scientific and clinical researches. This research is a cardiovascular disease include age, alteration of protein expression. A study of preliminary study that used proteomic technique for comparative protein expressions with genetic, lifestyle pattern and health proteome in rat off spring related to focusing on the molecular basis of CVD in HHcy condition. The aim of this study was to conditions such as hypertension, maternal food intake during pregnancy identify protein profiles between control (non-HHcy) group and three groups of HHcy. hyperlipidemia and hyperhomo- showed that protein expressions in Chemiluminescense immunoassay was used for determination of plasma homocysteine cysteinemia. Homocysteine is an amino homocysteine cycle was altered among rat levels. Protein expressions in human serum were determined and analyzed by gel acid, which results from methionine groups with different levels of electrophoresis and LC/MS-MS technique. Proteins were quantified by using DeCyderMS utilization in cells. The normal range of homocysteine intake1. Experimental analysis software. Jvenn was used for comparing protein lists with venn diagram. Function total plasma homocysteine in adult with studies revealed association between and class of protein were demonstrated by using PANTHER software. The interaction of fasting is usually in 5-15 µmol/L, and its protein expressions and genetic variations chemical and protein was predicted by using STITCH V.5.0. Eight unique proteins were up- level greater than 15 µmol/L is defined as which involved in homocysteine regulated in hyperhomocysteinemia groups, including MUC-19, LPAR1, MAPO2, hyperhomocysteinemia5. Elevated level of metabolism such as cystathionine β- GPALPP1, FECH, TUSC2, RBM38 and ANO1. Notably, RBM38, TUSC2, and ANO1 homocysteine can induce oxidative stress synthase (CBS)2 and paraoxonase1 found in subjects with plasma homocysteine levels more than 50 µmol/L were discovered and by disruption of anti-oxidant enzymes. (PON1) in induced mouse to linked to CVD risks via apoptotic signaling pathways. This study provided better insight into Simultaneously, it also initiates endothelial hyperhomocysteinemia condition3. There the interaction between expressed proteins profiles after post-translational modification cell injury through increased arterial was a report with alteration of which may resulted in induction of physiological alteration that may related to homocysteine- stiffness by decreased nitric oxide (NO) apolipoprotein A-I in patients carrying induced CVD. bioavailability6,7. Another mechanism of MTHFR C677T mutations in Italian Keywords: Hyperhomocysteinemia, Proteomics, Apoptosis hyperhomocysteinmia induced abnormal population4. A quantitative reduction of * Corresponding author molecular function involves apoptosis apolipoprotein A-I in mutant individuals Assist. Prof. Jintana Sirivarasai, Ph.D signaling pathway via endoplasmic with hyper-homocysteinemia was Graduate Program in Nutrition, Faculty of Medicine Ramathibodi Hospital, Mahidol University reticulum stress (ER stress). ER stress can E-mail: [email protected] associated with an increased risk of lead to alter function/ structure of proteins วารสารพิษวิทยาไทย 2561 ; 33(2) : 9-28 11 Apoptotic RBM38, TUSC2 and ANO1 Proteins in Human Serum Proteome Are Introduction cardiovascular diseases. Notably, they Associated with Hyperhomocysteinemia highlighted the novel association among Proteomics is one of the new era of Nuttawut Lainumngen1, Sittiruk Roytrakul2, Tuangrat Tangstheanphan3, Piyamitr genetic factor, homocysteine levels and Sritara4, Jintana Sirivarasai5* omics studies involving whole protein alteration of protein expression. However, expressions profiles in cells together with 1Master of Science Program in Nutrition, Faculty of Medicine Ramathibodi Hospital and Institute of Nutrition, previous proteomic studies of hyper- Mahidol University, Thailand quantify and identify the protein product 2 Genome Technology Research
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