High Throughput Screening of Polymeric Biomaterials For
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UNITED for People and Animals
NEWS May 2020 - Issue 125 UNITED for people and animals COVID-19 Research Updates Our incredible Journey & Impacts Protect the Animal Free Future Contents CHAIR OF THE BOARD .......................................... 3 FROM OUR PATRON .............................................. 4 MESSAGE FROM CEO ............................................ 5 OUR HISTORY ........................................................ 6 CELEBRATING 50 YEARS ....................................... 8 ARC 1.0 .................................................................10 ARC 2.0 .................................................................11 CURRENT PROJECTS ..........................................12 THE COVID-19 VACCINE PARADOX ..................14 CURRENT PROJECTS: COVID-19 .......................16 REVIEW .................................................................18 MEET THE SAP .....................................................20 PARTNERSHIPS ....................................................22 YOUR IMPACT FOR ANIMALS .............................24 FABULOUS FUNDRAISERS ..................................26 HOW YOU CAN HELP ..........................................28 SHOPPING ...........................................................30 FROM OUR PATRON ............................................31 BOARD OF TRUSTEES CHAIR: Ms Laura-Jane Sheridan VICE CHAIR: Ms Natalie Barbosa TREASURER: Mr Daniel Cameron Dr Christopher (Kit) Byatt Professor Amanda Ellison Ms Julia Jones COMPANY SECRETARY: Ms Sally Luther Animal Free Research UK SCIENTIFIC -
High Levels of Β-Xylosidase in Thermomyces Lanuginosus
b r a z i l i a n j o u r n a l o f m i c r o b i o l o g y 4 7 (2 0 1 6) 680–690 ht tp://www.bjmicrobiol.com.br/ Industrial Microbiology High levels of -xylosidase in Thermomyces lanuginosus: potential use for saccharification a,∗ a b c Juliana Moc¸o Corrêa , Divair Christi , Carla Lieko Della Torre , Caroline Henn , b b b José Luis da Conceic¸ão-Silva , Marina Kimiko Kadowaki , Rita de Cássia Garcia Simão a Centro de Ciências Exatas e Tecnológicas b Centro de Ciências Médicas e Farmacêuticas, UNIOESTE, Cascavel, PR, Brazil c Central Hidrelétrica de Itaipu, Itaipu Binacional, Foz do Iguac¸u, PR, Brazil a r t a b i c l e i n f o s t r a c t Article history: A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and Received 5 November 2015 its -xylosidases optimization in response to agro-industrial residues was performed. Using Accepted 20 February 2016 statistical approach as a strategy for optimization, the induction of -xylosidases activity Available online 27 April 2016 was evaluated in residual corn straw, and improved so that the optimum condition achieved  Associate Editor: Solange Ines high -xylosidases activities 1003 U/mL. According our known, this study is the first to show Mussatto so high levels of -xylosidases activities induction. In addition, the application of an experi- mental design with this microorganism to induce -xylosidases has not been reported until ◦ Keywords: the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 C ◦ showing better thermostability at 55 C. -
Physiology-2021-Abstract-Book.Pdf (Physoc.Org)
Physiology 2021 Our Annual Conference 12 – 16 July 2021 Online | Worldwide #Physiology2021 Contents Prize Lectures 1 Symposia 7 Oral Communications 63 Poster Communications 195 Abstracts Experiments on animals and animal tissues It is a requirement of The Society that all vertebrates (and Octopus vulgaris) used in experiments are humanely treated and, where relevant, humanely killed. To this end authors must tick the appropriate box to confirm that: For work conducted in the UK, all procedures accorded with current UK legislation. For work conducted elsewhere, all procedures accorded with current national legislation/guidelines or, in their absence, with current local guidelines. Experiments on humans or human tissue Authors must tick the appropriate box to confirm that: All procedures accorded with the ethical standards of the relevant national, institutional or other body responsible for human research and experimentation, and with the principles of the World Medical Association’s Declaration of Helsinki. Guidelines on the Submission and Presentation of Abstracts Please note, to constitute an acceptable abstract, The Society requires the following ethical criteria to be met. To be acceptable for publication, experiments on living vertebrates and Octopus vulgaris must conform with the ethical requirements of The Society regarding relevant authorisation, as indicated in Step 2 of submission. Abstracts of Communications or Demonstrations must state the type of animal used (common name or genus, including man. Where applicable, abstracts must specify the anaesthetics used, and their doses and route of administration, for all experimental procedures (including preparative surgery, e.g. ovariectomy, decerebration, etc.). For experiments involving neuromuscular blockade, the abstract must give the type and dose, plus the methods used to monitor the adequacy of anaesthesia during blockade (or refer to a paper with these details). -
The Use of Non-Human Primates in Research in Primates Non-Human of Use The
The use of non-human primates in research The use of non-human primates in research A working group report chaired by Sir David Weatherall FRS FMedSci Report sponsored by: Academy of Medical Sciences Medical Research Council The Royal Society Wellcome Trust 10 Carlton House Terrace 20 Park Crescent 6-9 Carlton House Terrace 215 Euston Road London, SW1Y 5AH London, W1B 1AL London, SW1Y 5AG London, NW1 2BE December 2006 December Tel: +44(0)20 7969 5288 Tel: +44(0)20 7636 5422 Tel: +44(0)20 7451 2590 Tel: +44(0)20 7611 8888 Fax: +44(0)20 7969 5298 Fax: +44(0)20 7436 6179 Fax: +44(0)20 7451 2692 Fax: +44(0)20 7611 8545 Email: E-mail: E-mail: E-mail: [email protected] [email protected] [email protected] [email protected] Web: www.acmedsci.ac.uk Web: www.mrc.ac.uk Web: www.royalsoc.ac.uk Web: www.wellcome.ac.uk December 2006 The use of non-human primates in research A working group report chaired by Sir David Weatheall FRS FMedSci December 2006 Sponsors’ statement The use of non-human primates continues to be one the most contentious areas of biological and medical research. The publication of this independent report into the scientific basis for the past, current and future role of non-human primates in research is both a necessary and timely contribution to the debate. We emphasise that members of the working group have worked independently of the four sponsoring organisations. Our organisations did not provide input into the report’s content, conclusions or recommendations. -
( 12 ) United States Patent
US010154979B2 (12 ) United States Patent ( 10 ) Patent No. : US 10 , 154, 979 B2 Remmereit et al. ( 45 ) Date of Patent : Dec . 18 , 2018 ( 54) LIPID COMPOSITIONS CONTAINING ( 56 ) References Cited BIOACTIVE FATTY ACIDS U . S . PATENT DOCUMENTS ( 71 ) Applicants : Jan Remmereit, Volda (NO ) ; Alvin 5 , 456 ,912 A 10 / 1995 German et al. Berger , Long Lake , MN (US ) 6 ,034 , 132 A * 3 / 2000 Remmereit . .. .. A61K 31/ 19 514 / 560 (72 ) Inventors : Jan Remmereit, Hovdebygda (NO ) ; 6 , 280 , 755 B1 8 / 2001 Berger et al. Alvin Berger , Long Lake, MN (US ) 2012 /0156171 A1 6 /2012 Breton et al. (73 ) Assignee : SCIADONICS , INC . , Long Lake, MN FOREIGN PATENT DOCUMENTS ( US ) EP 1685834 8 / 2006 EP 1685834 A1 * 8 / 2006 . .. A23D 9 / 00 ( * ) Notice : Subject to any disclaimer, the term of this FR 2756465 6 / 1998 patent is extended or adjusted under 35 JP 61058536 3 / 1986 WO 95 / 17897 7 / 1995 U . S . C . 154 (b ) by 0 days . WO W09517897 * 7 / 1995 WO WO 9517897 A1 * 7 / 1995 A61K 31/ 20 (21 ) Appl. No. : 14 /774 ,432 WO 96 / 005164 2 / 1996 WO 2006 /009464 1 / 2006 (22 ) PCT Filed : Mar. 10 , 2014 wo WO 2006009464 A2 * 1 / 2006 .. .. A61K 31/ 10 (86 ) PCT No . : PCT/ US2014 / 022553 OTHER PUBLICATIONS § 371 ( C ) ( 1 ) , Smith et al. ( Caltha palustris L . Seed oil . A Source of Four Fatty ( 2 ) Date : Sep . 10 , 2015 Acids with cis - 5 -Unsaturate ; Lipids, vol. 3 , No . 1 , Sep . 7 , 1967 ) . * Barnathan et al . “ Non -methylene - interrupted fatty acids from marine (87 ) PCT Pub . No .: WO2014 /143614 invertebrates : Occurrence , characterization and biological proper ties ” BIOCHIMIE , vol. -
Thermophilic Fungi: Taxonomy and Biogeography
Journal of Agricultural Technology Thermophilic Fungi: Taxonomy and Biogeography Raj Kumar Salar1* and K.R. Aneja2 1Department of Biotechnology, Chaudhary Devi Lal University, Sirsa – 125 055, India 2Department of Microbiology, Kurukshetra University, Kurukshetra – 136 119, India Salar, R. K. and Aneja, K.R. (2007) Thermophilic Fungi: Taxonomy and Biogeography. Journal of Agricultural Technology 3(1): 77-107. A critical reappraisal of taxonomic status of known thermophilic fungi indicating their natural occurrence and methods of isolation and culture was undertaken. Altogether forty-two species of thermophilic fungi viz., five belonging to Zygomycetes, twenty-three to Ascomycetes and fourteen to Deuteromycetes (Anamorphic Fungi) are described. The taxa delt with are those most commonly cited in the literature of fundamental and applied work. Latest legal valid names for all the taxa have been used. A key for the identification of thermophilic fungi is given. Data on geographical distribution and habitat for each isolate is also provided. The specimens deposited at IMI bear IMI number/s. The document is a sound footing for future work of indentification and nomenclatural interests. To solve residual problems related to nomenclatural status, further taxonomic work is however needed. Key Words: Biodiversity, ecology, identification key, taxonomic description, status, thermophile Introduction Thermophilic fungi are a small assemblage in eukaryota that have a unique mechanism of growing at elevated temperature extending up to 60 to 62°C. During the last four decades many species of thermophilic fungi sporulating at 45oC have been reported. The species included in this account are only those which are thermophilic in the sense of Cooney and Emerson (1964). -
The Interplay Between Biomaterial Degradation and Tissue Properties : Relevance for in Situ Cardiovascular Tissue Engineering
The interplay between biomaterial degradation and tissue properties : relevance for in situ cardiovascular tissue engineering Citation for published version (APA): Brugmans, M. C. P. (2015). The interplay between biomaterial degradation and tissue properties : relevance for in situ cardiovascular tissue engineering. Technische Universiteit Eindhoven. Document status and date: Published: 01/01/2015 Document Version: Publisher’s PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication: • A submitted manuscript is the version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. -
Assembly of Lipase and P450 Fatty Acid Decarboxylase to Constitute A
Yan et al. Biotechnology for Biofuels (2015) 8:34 DOI 10.1186/s13068-015-0219-x RESEARCH ARTICLE Open Access Assembly of lipase and P450 fatty acid decarboxylase to constitute a novel biosynthetic pathway for production of 1-alkenes from renewable triacylglycerols and oils Jinyong Yan1*, Yi Liu1,2, Cong Wang1, Bingnan Han3 and Shengying Li1* Abstract Background: Biogenic hydrocarbons (biohydrocarbons) are broadly accepted to be the ideal ‘drop-in’ biofuel alternative to petroleum-based fuels due to their highly similar chemical composition and physical characteristics. The biological production of aliphatic hydrocarbons is largely dependent on engineering of the complicated enzymatic network surrounding fatty acid biosynthesis. Result: In this work, we developed a novel system for bioproduction of terminal fatty alkenes (1-alkenes) from renewable and low-cost triacylglycerols (TAGs) based on the lipase hydrolysis coupled to the P450 catalyzed decarboxylation. This artificial biosynthetic pathway was constituted using both cell-free systems including purified enzymes or cell-free extracts, and cell-based systems including mixed resting cells or growing cells. The issues of high cost of fatty acid feedstock and complicated biosynthesis network were addressed by replacement of the de novo biosynthesized fatty acids with the fed cheap TAGs. This recombinant tandem enzymatic pathway consisting of the Thermomyces lanuginosus lipase (Tll) and the P450 fatty acid decarboxylase OleTJE resulted in the production of 1-alkenes from purified TAGs or natural oils with 6.7 to 46.0% yields. Conclusion: Since this novel hydrocarbon-producing pathway only requires two catalytically efficient enzymatic steps, it may hold great potential for industrial application by fulfilling the large-scale and cost-effective conversion of renewable TAGs into biohydrocarbons. -
Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production
processes Article Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production Marina Tišma 1,*, Toma Tadi´c 1, Sandra Budžaki 1, Marta Ostojˇci´c 1, Anita Šali´c 2, Bruno Zeli´c 2 , Nghiep Nam Tran 3,4 , Yung Ngothai 3 and Volker Hessel 3 1 Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Osijek HR-31000, Croatia 2 Faculty of Chemical Engineering and Technology, University of Zagreb, Zagreb HR-10000, Croatia 3 School of Chemical Engineering, The University of Adelaide, North Terrace Campus, Adelaide 5005, Australia 4 School of Chemical Engineering, Can Tho University, Campus 2, Can Tho 900000, Vietnam * Correspondence: [email protected]; Tel.: +385-31-224-358 Received: 13 June 2019; Accepted: 17 July 2019; Published: 19 July 2019 Abstract: This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 ◦C, t = 9 days). Lipase showed optimum activity at T = 40 ◦C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 ◦C. During the experiments, the operational stability of the enzyme was studied. -
Evaluation of a Novel Thermo-Alkaline Staphylococcus Aureus Lipase for Application in Detergent Formulations
Saudi Journal of Biological Sciences (2016) xxx, xxx–xxx King Saud University Saudi Journal of Biological Sciences www.ksu.edu.sa www.sciencedirect.com ORIGINAL ARTICLE Evaluation of a novel thermo-alkaline Staphylococcus aureus lipase for application in detergent formulations Abir Ben Bacha a,b,*,1, Alaa Al-Assaf a, Nadine M.S. Moubayed c, Islem Abid c a Biochemistry Department, Science College, King Saud University, P.O Box 22452, Riyadh 11495, Saudi Arabia b Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, Sfax 3038, Tunisia c Botany and Microbiology Department, Science College, King Saud University, P.O Box 22452, Riyadh 11495, Saudi Arabia Received 27 April 2016; revised 24 August 2016; accepted 3 October 2016 KEYWORDS Abstract An extracellular lipase of a newly isolated S. aureus strain ALA1 (SAL4) was purified ° Staphylococcus aureus lipase; from the optimized culture medium. The SAL4 specific activity determined at 60 C and pH 12 Purification; by using olive oil emulsion or TC4, reached 7215 U/mg and 2484 U/mg, respectively. The 38 Characterization; NH2-terminal amino acid sequence of the purified enzyme starting with two extra amino acid resi- Thermo-alkaline; dues (LK) was similar to known staphylococcal lipase sequences. This novel lipase maintained Detergent-stable almost 100% and 75% of its full activity in a pH range of 4.0–12 after a 24 h incubation or after 0.5 h treatment at 70 °C, respectively. Interestingly, SAL4 displayed appreciable stability toward oxidizing agents, anionic and non-ionic surfactants in addition to its compatibility with several commercial detergents. -
“But It's Just a Fish”: Understanding the Challenges of Applying the 3Rs
animals Article “But It’s Just a Fish”: Understanding the Challenges of Applying the 3Rs in Laboratory Aquariums in the UK Reuben Message * and Beth Greenhough School of Geography and the Environment, University of Oxford, Oxford OX1 2JD, UK; [email protected] * Correspondence: [email protected] Received: 29 October 2019; Accepted: 28 November 2019; Published: 3 December 2019 Simple Summary: Fish are widely used in research and some species have become important model organisms in the biosciences. Despite their importance, their welfare has usually been less of a focus of public interest or regulatory attention than the welfare of more familiar terrestrial and mammalian laboratory animals; indeed, the use of fish in experiments has often been viewed as ethically preferable or even neutral. Adopting a social science perspective and qualitative methodology to address stakeholder understandings of the problem of laboratory fish welfare, this paper examines the underlying social factors and drivers that influence thinking, priorities and implementation of fish welfare initiatives and the 3Rs (Replacement, Reduction and Refinement) for fish. Illustrating the case with original stakeholder interviews and experience of participant observation in zebrafish facilities, this paper explores some key social factors influencing the take up of the 3Rs in this context. Our findings suggest the relevance of factors including ambient cultural perceptions of fish, disagreements about the evidence on fish pain and suffering, the language of regulators, and the experiences of scientists and technologists who develop and put the 3Rs into practice. The discussion is focused on the UK context, although the main themes will be pertinent around the world. -
Animals in Science Regulation Unit Annual Report 2015
Animals in Science Regulation Unit Annual Report 2017 © Crown copyright 2018 This publication is licensed under the terms of the Open Government Licence v3.0 except where otherwise stated. To view this licence, visit http://nationalarchives.gov.uk/doc/open-government- licence/version/3/ or write to the Information Policy Team, The National Archives, Kew, London TW9 4DU, or email: [email protected]. Where we have identified any third party copyright information you will need to obtain permission from the copyright holders concerned. ISBN: 978-1-78655-740-7 © Crown copyright 2018 This publication is licensed under the terms of the Open Government Licence v3.0 except where otherwise stated. To view this licence, visit http://nationalarchives.gov.uk/doc/open-government- licence/version/3/ or write to the Information Policy Team, The National Archives, Kew, London TW9 4DU, or email: [email protected]. Where we have identified any third party copyright information you will need to obtain permission from the copyright holders concerned. ISBN: 978-1-78655-757-5 Contents Ministerial foreword 4 Section 6: Inspection 19 Inspection 19 Foreword 5 Baseline setting 19 Risk management 20 Section 1: What the Animals in Science Regulation Inspector training and continuous professional Unit does 7 development 20 The Policy and Administration Group 7 Inspection reporting 21 Investigating allegations made to the Animals in Science Regulation Unit 21 Section 2: The regulatory framework 9 Judicial Reviews 9 Section 7: Compliance 22