Supporting Information for Proteomics DOI 10.1002/pmic.200600043

Yuzo Nozu, Akira Tsugita and Kenichi Kamijo

Proteomic analysis of rice leaf, stem and root tissues during growth course

ª 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.com Supplementary Table 1. Observed unique leaf, stem and root proeins during the course of growth.

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 catalase isozyme A X X X X X X X X superoxide dismutase, [Mn] X X X X X X X X L-ascorbate peroxidase AKNYPVV/ X X X X X X X X actin 1 X X X X X X X X ascorbate peroxidase X X X X X X X X peroxiredoxin APVAVXD/ X X X X X X X X glyoxalase I X X X X X X X X ATP synthase beta subunit, mitochondrial X X X X X X X X cytosolic glyceraldehyde-3-phosphate dehydrogenase GKIKIXI/ X X X X X X X X cytosolic glyceraldehyde-3-phosphate dehydrogenase VALQSEL/ X S-adenosyl methionine synthetase 1 X X X X X X X X fructose-bisphosphate aldolase isoenzyme C-1 X X X X X X X X cysteine synthase X X X X X X X X plastidic cysteine synthase 1 AVAAEVE/ X X X X X X X X nucleoside diphosphate kinase X X X X X X X X malate dehydrogenase SSASGQP/ X X X X X X X X 2,3-bisphosphoglycerate-independent phosphoglycerate mutase X X X X X X X X fructokinase II PLGDGAA/ X X X X X X X X cytosolic triosephosphate isomerase GRKFXVG/ X X X X X X X X cytosolic triosephosphate isomerase (#46->) YVFLPXY/ cytosolic triosephosphate isomerase (#34->) STDVVEV/ mitochondrial chaperonine-60 kDa DAKDIAF/ X X X X X X X X

- 1 - isoflavone reductase X X X X X X X X Rubisco acrtivase (#46->) KELDEGK/ X X X X X X X X Rubisco acrtivase (#87->) FQAPTGD/ X X X X X X X X Rubisco large subunit X X X X X X X X Rubisco large subunit (#180->) LSAKNYG/ X Rubisco large subunit (#320->) XSGXDHI/ X ＦＡＲＥＬＧＶ Rubisco large subunit (#256->) X / Rubisco large subunit (#41->) RVTPQXX/ X ATP synthase beta subunit MRTNPTT/ X X X X X X X X glyceraldehyde-3-phosphate dehydrogenase AKIKIGI/ X X X X X X X glyceraldehyde-3-phosphate dehydrogenase SKIKIXI/ EFVVESG/ glyceraldehyde-3-phosphate dehydrogenase (#143->) nucleoside diphosphate kinase I X X X X X X X formate dehydrogenase AHTSAGS/ X X X X X X X X chaperonin 60 kDa beta subunit AKELHFN/ X X X X X X X X translational elongation factor Tu ARGKFER/ X X X X X X X X heat shock protein 70 kDa AVQAAIL/ X X X X X cyclophilin 2 X X X X X heat shock 70 kDa protein, mitochodrial X X X X X heat shock protein cognate 70 kDa X X reversibly glycosylated polypeptide X X X X X X tubulin beta chain MREILHI/ X X X X X actin depolymerizing factor X X X X X glycine-rich protein X X X X X osr40c1 X X X X

- 2 - glutamine synthetase shoot isozyme X X X X X S-adenosyl methionine synthetase 2 X X X X X glyoxysomal malate dehydrogenase RAKGXAP/ X X X X cytoplasmic malate dehydrogenase AKEPMRV/ X X X X X X dihydrolipoamide dehydrogenase AAVAGEE/ X X X X X dehydrogenase ALREDGP/ X X X X X X reductase X X X X X chalcon isomerase X X X X dihydrolipoamide acyltransferase ASDNGDQ/ X X X X glutamate dehydrogenase X X X X X coproporphyrinogen III oxidase X X X X aspartate aminotransferase ALAVDVX/ X X X X X X aminomethyl transferase AGAAEAA/ X X X X malate dehydrogenase SAAAAAE/ X X X 20S proteasome subunit alpha-6 X X X X X X 20S proteasome subunit alpha-5 X X X X X X disulfide-isomerase DDVLALT/ X X X X X voltage-dependent anion channel VGPGLYP/ X X X X voltage-dependent anion channel X X X X X X H+exporting ATPase X X X X X X X translationally controlled tumor protein MLVYQDL/ X X X X X abscisic acid- and stress-induced protein QGVDSYG/ X X X X X glutathione peroxidase 1 X X X X methylmalonate-semialdehyde dehydrogenase X X X X cystathionine gamma-synthase X X dehydroascorbate reductase X X X X X disulfide isomerase X X X X X X

- 3 -

26S protasome regulatory particle triple-A ATPase subunit 3 X X X X X X nascent polypepteide associated complex alpha chain X X X X X X elongation factor 1-beta' X X X X salt-stress induced protein TLVKIXP/ X X X X drought-induced S-like ribonuclease DSPFDFY/ X X X X X X glutathione S-transferase X X X X X carboxymethylene butenolidase X X dnaK-type molecular chaperone hsp 70 kDa X X X thioredoxin H-type X X X X X proteasome endopeptidase complex alpha chain X X X X dnaK-type molecular chaperone Bip (K)EXTKKL/ X X X X 20S proteasome subunit beta-1 X X X 20S proteasome subunit alpha-2 X X X X X 20S proteasome subunit beta-3 X X

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 glutathione S-transferase II APMKLYG/ X X X X 20S proteasome beta 6 subunit X X X X superoxide dismutase, [Cu-Zn] ATKTAVA/ X X X X X X X X Rubisco small subunit XQVXPIE/ X X X X X X X X photosystem II oxygen-evolving complex protein 1 EGVPRRL/ X X X X X X X X X photosystem II oxygen-evolving complex protein 2 AYGEAAN/ X X X X X X X phosphoribulokinase SVDKPVV/ X X X X X X X X VSTETTG/ glutamine synthetase shoot isozyme, chloroplast X X X X X X X X

- 4 - chloroplast fructose-bisphosphate aldolase GAXDDEL/ X X X X X X X X 3-beta hydroxysteroid dehydrogenase/isomerase X X X X X X X X peptidyl-prolyl cis-trans isomerase X X X X X X X X 50S ribosomal protein L10 X X X X X X X X glycine dehydrogenase VSASALA/ X X X X X X ferredoxin-NADP reductase, leaf isozyme X X X X X X ribulose-phosphate 3-epimerase XSRVDKF/ X X X X X X X glycolate oxidase X X X X X X glyceraldehyde-3-phosphate dehydrogenase KLKVAIN/ X X X X X X X chloroplast photosystem I reaction center subunit II X X X X X X ATP synthase gamma-subunit X X X X X X X quinone oxidoreductase X X X X X X X sedoheptulose-1,7-bisphophatase EIGDSLE/ X X X X X X ribosomal protein X X X X X X X thioredoxin M-type X X X X X X ferritin X X X X r40c1 protein X X X germin-like protein 5 LTQDFXV/ X X X X X X transketolase AAVETLE/ X X triose phosphate isomerase, chloroplast AGSGXFF/ X X X epimerase/dehydratase X X X X ATP dependent Clp protease ATP-binding subunit XFERFTE/ X X X 70 kDa peptidylpropyl isomerase X X X chloroplast peptidyl-prolyl cis-trans isomerase VLISGPG/ X X

- 5 -

20 kDa chaperonin, chloroplast ATVVTXK/ X X ribosomal protein SWDGPLS/ X X X X thioredoxin peroxidase X X X salt-induced protein X X X X X X heat shock-related protein X X X X glyceraldehyde-3-phosphate dehydrogenase X X X X ATP synthetase epsilon subunit X X peroxidase X X cell death associated protein X X thioredoxin peroxidase AGGVDDA/ X X photosystem I 9 kDa protein (fragment) X X oxalate oxidase X X X cytosolic monodehydroascorbate reductase GADSKNI/ X X transcription factor APFI X X 3'(2'),5'bisphosphate nucleotidase X X caffeoyl-CoA O-methyltransferase 1 X X X cytosolic 6-phosphogluconate dehydrogenase X X X enoyl-[acyl-career-protein] reductase AXESGPQ/ X X X UDP-glucuronic acid decarboxylase X X X aldehyde dehydrogenase X X X eukaryotic initiation factor 4A X X X 60S acidic ribosomal protein P0 X X X mannose-binding rice lectin X X X enolase X X UDP-glucose pyrophosphorylase X X 26S proteasome regulatory subunit 6A X X X prepro-glutelin X X X

- 6 - translation initiatiom factor 5A X X X 40S ribosomal protein S12 X X X DEAD BOX RNA helicase X X X GF14-c protein X X GDP dissociation inhibitor protein X X alpha-tubulin X X Sgt1 X fatty acid alpha-oxidase X cysteine synthase X X adenosine kinase X X 3-isopropylmalate dehydrogenase AAKRSYN/ X X Rubisco subunit binding-protein beta subunit AKELYFN/ X caffeic acid 3-O-methyltransferase STAADMA/ X X 26S proteasome regulatory particle triple-A ATPase subunit 6 X vacuolar ATPase B subunit X X prohibitin homologue X superoxide dismutase, (Cu-Zn) 1 X secretory peroxidase X receptor-like protein kinase X X guanine nucleotide-binding protein beta subunit X r40g2 protein AAPSDXN/ X X ATP synthase alpha chain, mitochondrial X X ATP synthase delta' chain, mitochondrial X X alpha-soluble NSF attachment protein X isopentenyl pyrophosphate:dimethylallyl pyrophosphate isomerase X methylenetetrahydrofolate dehydrogenase X X

- 7 -

UDP-glucose dehydrogenasee X X cysteine synthase, 5'-partial X glycine hydroxy methyltransferase X carbonic anhydrase/acetyltransferase X X inorganic pyrophosphatase X

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 phosphoenolpyruvate phosphonomutase X xyloglucan endotransglycosylase X 26S proteasome regulatory particle triple-A ATPase subunit 4 X X 20S proteasome subunit alpha-1 X X ６０ kDa chaperonin alpha subunit X endoplasmic reticulum membrane fusion protein X

ATPase with chaperone activity X aminoacylase X translational elongation factor Tu, mitochondrial X susceptibility homeodomain transcription factor X X nuclear transport factor 2 X gamma hydroxybutylate dehydrogenase X 20S proteasome subunit alpha-3 X

X mitochondrial processing peptidase alpha-II chain elongation factor-1 alpha X X X X X

- 8 - phosphoserine aminotransferase X 40S ribosomal protein S21 X orthophosphate dikinase X X X chaperonine 60 kDa alpha subunit X X X peroxidase X 1,4 benzoquinone reductase AVKVYVV/ X X porin-like protein X peroxidase X IAA-amino acid hydrolase X tyrosine phosphatase X X X X X GTP-binding protein X X X X mRNA binding protein X X X X X peptidyl-prolyl cis-trans isomerase X X X X heat shock protein HSP 70 kDa homolog* DRQALXX/ X heat shock protein 26 kDa X heat shock protein 70 kDa EKVVGIL/ X X zinc-binding protein DAHKSEV/ X guanine nucleotide-binding protein beta subunit X calcium-dependent protein kinase (fragment) RPVAVXD/ X GTP-binding protein SUP1,EF-1-alpha-related SQADIXA/ X calcium-dependent protein kinase X ethylene responsive factor X tau-tubulin kinase family member* RFTKGXX/ X ethylene-inducible protein X X receptor-like protein kinase (fragment) SVSXAQI/ X ferrichrome-binding periplasmic protein* SYNFANK/ X

- 9 - spermidine/putrescine-binding periplasmic protein* X AYRVDKF/ InsB from Escherichia coli SAEMDEQ/ X bir1610* AGAQTAT/ X high-glucose-regulated protein 8 HQQGVGA/ X photosystemⅠlight harvesting complex Ⅲ/b AEEEAAA/ X X photosystem II 11 kDa protein related ATIEFXX/ X K+transporting ATPase, C chain* ATADPKK/ X cytochrome P450 AASGAAV/ X cysteine synthase X cob(1)alamin adenosyltransferase* SVQDAEV/ X phytoene dehydrogenase X ribulose-5-phosphate 3-epimerase* QQLEALX/ X aminotransferase* TAELRPK/ X monodehydroascorbate reductase (NADH) AADSKNI/ X fructose-1,6-bisphosphatase X tetrahydrodipicolinate N-succinyltransferase* AYVDSGX/ X dehydrogenases* MQTXPKA/ X (S)-2-hydroxy-acid oxidase X acyl-CoA dehydrogenase family* XTEDXKN/ X glycoside hydrolase family* ALIEAVX/ X limonene cyclase-like protein SLYXPDT/ X glutamate-1-semialdehyde aminotransferase SPGVPKL/ X acidic ribosomal protein PO AIKRTKA/ X elongation factor Tu GTP binding domain* DTEKVVK/ X chloroplast 50S ribosomal protein L6 X X

- 10 -

40S ribosomal protein S2* HQVKDXK/ X mudrA protein GQPXAEK/ X reverse transcriptase SIHAAMH/ X homeodomain transcription factor RTIKPYL/ X transcription activator AELDGEX/ X peptide transporter X vesicle soluble NSF attachment protein receptor LKQKAXV/ X ATPase* RLXDPDR/ X phosphate ABC transporter* RFAVNVL/ X peroxidase X plasma membrane polypeptide X glycine-rich RNA-binding protein X Rieske Fe-S protein X X photosystem II protein W AARPAIQ/ X X X X oxygen evolving complex protein X X hydrolase X X X X alanine aminotransferase X X X X thioredoxin X ribosomal protein L12 X X parvalbumin (fragment) X glutathione S-transferase X heme binding protein 2 X X gibberellin-20 oxidase X 10-deacetylbaccatin III-10-O-acetyl transferase X

Protein identificationa) N-terminalb) c)

- 11 -

sequence L1 L2 L4 L8 L10 S2 S4 S8 O-deacetylbaccatin III-10-O-acetyltransferase X sarcosine oxidase X Bowman-Birk proteinase inhibitor X SR-rich pre-mRNA splicing activator X disease resistant protein WNPTSVS/ X chitinase X thioredoxin peroxidase TIAVGDK/ X heat shock 70 kDa protein* ASTNARX/ X CdK-activating protein kinase PQPASSW/ X calmodulin-binding protein GPALGQP/ X S-domain receptor-like protein kinase DAKGGAF/ X serine/threonine protein kinase DVIKIAV/ X GTP-binding protein X nicotinic acetylcholine receptor gamma subunit* X GAHTLEW/ signal transduction histidine kinase* DLPATRE/ X CDPK-substrate protein 1 GNQLYNS/ X membrane trafficking factor AQPEPVV/ X glutathione S-transferase X X membrane protein* WQGYGYS/ X endosomal protein-like DLPMFRI/ X outer membrane protein* GPVAVGX/ X toxin A* SKFLTLG/ X nitrogen assimilation regulatory protein* VLIXGPS/ X BKRF1 encodes EBNA-1 protein GVPSKPR/ X

- 12 -

NPH3 domain containing protein SRXWFED/ X pentatricopeptide repeat-containing protein-like X GLLPPSH/ cytochrome-C oxidase chainⅡ MKFEWL/ X glycerol-3-phosphate dehydrogenase* SLAVPMQ/ X phosphoenolpyruvate carboxykinase* SAVAREA/ X DMSO reductase anchor subunit* DLSNLTP/ X acetyl-CoA oxidase ARNQPVG/ X 6-phosphogluconolactonase DRGKIRV/ X N-carbomyl-L-amino acid aminohydrase GLQNQXS/ X UDP glucose dehydrogenase GAYEPGL/ X D-isomer specific 2-hydroxyacid dehydrogenase X PA-phosphatase related phosphoesterase* KVHEAQX/ X S-adenosylmethionine:2-dimethylmenaquinone methyltransferase X riboflavin synthase alpha chain GLPQSFR/ X nucleotide-sugar dehydrogenase* AGPAELE/ X 2,3-dihydroxybiphenyl 1,2-dioxygenase* DQRTWX/ X casein kinase (fragment) GKYRDNX/ X quinolinate synthase* DPVLQAV/ X acyl-CoA dehydrogenases* GAXRVPL/ X monophenol monooxygenase* GANRDER/ X glucosyltransferase-2 WLQFMSR/ X beta-ketoacyl-CoA synthase SAPALAQ/ X N-acetylmuramoyl-L-alanine amidase* GNQVXNQ/ X chaperonin 21 kDa, chloroplast ATVVSPK/ X subtilisin-like proteinase GSYSATA/ X

- 13 - subtilisin homolog GXPESAR/ X 20S proteasome alpha subunit C1 X ubiquitin-conjugating enzyme DSNVFEW/ X ribosomal protein L14 X lysyl-tRNA synthetase* ALIVAGR/ X ribosomal protein L18 X histidyl-tRNA synthetase* IKTPTIXI/ X arginyl-tRNA synthetase* DXELYFX/ X DNA-binding protein KLAASMH/ X DNA-binding protein GSPRGSE/ X DNA-binding protein creA* SKPATEE/ X zinc finger (C2H2 type) protein 2 GQPWLKR/ X cyclic nucleotide and calmodulin-regulated ion channel SLPSALR/ X K+ effluxantiporter DWPFPD/ X ribose transport system permease protein* HQPLPRS/ X chitinase X chitinase X isoprenoid biosynthesis-like protein X GTP-binding regulatory protein X chloroplast drought-induced stress protein X 1-aminocyclopropane-1-carboxylate oxidase X germin-like protein 2 X methionine sulfoxide reductase X beta-amylase, chloroplast-targeted X alcohol dehydrogenase X Bowman-Bark type trypsin inhibitor X chelatase X X X

- 14 - senescence-associated protein-related IGLQNKN/ X auxin-responsive GH3 family protein QVEFVAI/ X auxin response factor 6a XXETDEV/ X peroxidase ELSVDYY/ X phytochrome A specific signal transduction component ALKMEEV/ X glutathione S-transferase X integral membrane protein* VLAVVVL/ X enolase* XLSELEK/ X 24 kDa protein 4413 LTQDFGV/ X bir5174* SAPASAA/ X lipoprotein* DQTQAXX/ X fruit protein AVQQDAX/ X glyceraldehyde-3-phosphate dehydrogenase X cytochrome b subunit of formate dehydrogenase* X QGEXGYI/

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 doxorubicin biosynthesis enzyme* ATVVSXP/ X UDP-glucose pyrophosphorylase X end-xyloglucan transferase AGFYEKF/ X adenine deaminase* XTAVVAE/ X UDP-glucose glucosyltransferase TVDVADE/ X hexokinase II X oxidase X O-deacetylbaccatin III-10-0-acetyltransferase X

- 15 - triosephosphate isomerase ARNFFVG/ X adenylosuccinate lyase SEDIXNL/ X 3-oxoacyl-[acyl-carrier-protein] reductase* AITRGVG/ X cytochrome P460* LSSNDIR/ X invertase SAASQQX/ X 5'-phosphoribosyl-5-aminoimidazole synthetase X adenosine kinase X 2-methylthioadenine synthetase* QVXATEE/ X peptidyl-tRNA hydrolase* AEYEATX/ X arginosuccinate synthase* QGDELXT/ X histidinol dehydrogenase* XQVDXIR/ X solution structure of 3-methyladenine DNA glycosylase I XAASVAP/ X glycosyl transferase*: QPDTVNV/ X urocanate hydratase* AEVDXSR/ X serine carboxypeptidase II-like protein DKRTAEX/ X disulfide-isomerase EEAAAAE/ X chloroplast peptidyl-prolyl cis-trans isomerase X translation initiation factor IF-2, chloroplas* X DNLEFNX/ elongation factor 1-BETA X histidyl-tRNA synthetase XPVLLDF/ X eukaryotic translation initiation factor 3, subunit 1 alpha, 35 kDa XLDDPAL/ X GTPase - translation elongation factor* AEAGGST/ X ATP-dependent RNA helicase X transcription factor VTIVAVP/ X

- 16 - zinc knuckle (CCHC-type) family protein DGVDSLK/ X transcription regulator of the Lacl family* ATENRE(A)/ X zinc finger (C3HC4-type Ring finger) family protein X DYYESLK/ regulator of chromosome condensation family protein X XKNIFVV/ zinc finger (C2H2-type) family protein XSDFSTT/ X tetR-family transcriptional regulator* AISHNKP/ X ribonucleoprotein X X sigma-54 dependent transcriptional regulator* X ALLAATE/ DNA-directed RNA polymerase, beta subunit* TLREDGX/ X plasma membrane protein-ATPase gene OSA3 X X mitochondrial substrate carrier family protein X NFQTAFK/ ammonium transporter ATAAVEP/ X vacuolar proton-ATPase AFGDXIT/ X catalase isozyme B X chitinase X pathogenesis-related protein X X peroxidase X chitinase III X X pathogenesis-related protein X aldo/keto reductase X glutathione S-transferase X chloroplast thylakoid lumenal 21.5 kDa protein X

- 17 - plasma membrane polypeptide X heme-binding protein X photosystem I reaction center subunit IV X X cytochrome b5 reductase X cytosolic fructose-1,6 -bisphophatase X fructokinase I X 1,4-benzoquinone reductase X X aminotransferase AGD2 X disulfide-isomerase X chloroplast chaperonin X eukaryotic translation initiation factor 1A X translation elongation factiors X chloroplast 29 kDa ribonucleoprotein A X potassium channel beta subunit protein X peroxidase X selenium binding protein X HSP 70 kDa X alpha-crystallin-type heat shock protein X Ras-related protein RIC1 X receptor-like protein kinase X lipoamide dehydrogenase X allene oxide synthase X aspartate-semialdehyde dehydrogenase X alanine aminotransferase X ribose 5-phosphate isomerase X germin protein type 1 X peptidase_M16_C X

- 18 - cytosolic glyceraldehyde 3-phosphate dehydrogenase H+transporting ATP synthase RAB24 protein PGLTLGD/ glutamine synthetase root isozyme alanine aminotransferase glucan endo-1,3-beta-D-glucosidase aspartate aminotransferase, mitochondrial dihydrolipoamide dehydrogenase disulfide-isomerase

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 miotic recombination protein* KVNFAET/ cyclin-like F-box family member* QPTNXKN/ alcohol dehydrogenase 1 peroxidase peroxidase disease resistance protein pathogen-related protein peroxidase LQLGYYK/ peroxidase peroxidase peroxidase peroxidase pathogenesis-related protein MKSLQGE/ pathogen-related protein type-1 disease resistance response protein DQXEITM/

- 19 - superoxide dismutase, (Cu-Zn) sodB VKAVAVL/ thaumatin-like protein ATFTITN/ superoxide dismutase, (Cu-Zn) sodA VKAVVVL/

XPDQLRV/ catalase hydroperoxidase 1* chitinase EQXGAQA/ submergence induced protein 2A iron binding protein* QYPKNIS/ dihydroorotate dehydrogenase pathogenesis-related protein NBS-LRR-like protein immunophilin GFEKTIL/ gamma tubulin glyoxalase II 14 kDa prolamin precursor RFDALSG/ mRNA export protein QPQPAXX/ glutathione S-transferase* STLIVEX/ glutathione S-transferase phosphomannomutase, eukaryotic MDO-like protein* HVFGMXL/ PRL1-interacting factor K XFALFKG/ 6Fe-6S prismane cluster-containing protein* XSTELAY/ bir7925* SLGSAAE/ r40c2 protein TIGGVPS/ S1-P1_nuclease late embryogenesis abundant protein NADP-specific isocitrate dehydrogenase

- 20 - cytosolic pyruvate orthophosphate dikinase pyruvate dehydrogenase E1 beta subunit pyrophosphate-fructose 6-phosphate 1-phosphotransferase ATP synthase AKEADXT/ oxidoreductase ubiquinol-cytochrome C reductase (fragment) APVSISD/ cytochrome oxidase 1* ADPILYQ/ root ferredoxin-NADP reductase SVQQASE/ cytochrome b5 reductase EEKGEDA/ oxidoreductase, FAD-binding ADKLLVG/ ATP synthase F0 subunit 1 MEFSPXA/ cytochrome c oxidase subunit III* XKGVLPG/ oxidoreductase, aldo/ketoreductase family* QGLTLID/ ATP synthase D chain, mitochondrial quinone-oxidoreductase Fe-S oxidoreductase* XVNFTDV/ pyruvate dehydrogenase E1 beta subunit isoform 1 SAAAKEM/ NAD-dependent isocitrate dehydrogenase TYMPXPG/ NADH-ubiquinone oxidoreductase STALNYH/ Fe-S oxidoreductase* KFYNAVP/ ATP synthase beta chain ATAAAAK/ cytosolic glutathione reductase alpha amylase hydrolase beta 1,3-glycosyltransferase

- 21 -

D-isomer specific 2-hydroxyacid dehydrogenase beta-1,3-glucanase GEIGVXY/ germin protein 4 aldose 1-epimerase alpha-galactosidase isocitrate/isopropylmalate dehydrogenase gamma-glutamylcysteine synthetase 1-aminocyclopropane-1-carboxylate synthase 1 2-hydroxypenta 2,4-dienoate hydratase* MLPELIQ/ fructose-1, 6-bisphosphatase AVDTAAA/ ornithine decarboxylase TAAAXAE/ alpha-galactosidase FENGLGR/ 3-isopropylmalate dehydratase XIPAEYA aldehyde dehydrogenase ALDH2b TAAVAEE/ threonine dehydrratase/deaminase ketol-acid reductoisomerase* VPPATXD/ phosphoribosylformimino-5-aminoimidazole carboxyamide ribotide isomerase* MKIIPVA/ exoglucanase carbamoyl phosphate synthetase small subunit 4-coumarate:coenzyme A ligase AVVTPAQ/ aminotrnsferase endo-beta-1,4-glucanase HLGKGGG/ beta-alanine synthases peptidyl-prolyl cis-trans isomerase* ALPAXNA/

Protein identificationa) N-terminalb) c)

- 22 -

sequence L1 L2 L4 L8 L10 S2 S4 S8 xylulose kinase galactosamine-6-phosphate isomerase* SGGASLX serine hydroxymethyltransferase* SQAXAAV/ S-adenosylmethionine-dependent methyl transferase* LAXDENL/ IAA amidohydrolase aminotransferase* DGDQVVA/ betaine aldehyde dehydrogenase GLFV_dehydrogenase alpha-galactosidase succinyl-CoA ligase alpha subunit EAMEAEL/ cytochrome P450 IAA amidohydrolase transferase dihydropyrimidinase ketol-acid reductoisomerase 3-dehydroquinate dehydratase cinnamyl alcohol dehydrogenase epimerase/dehydratase glutamine synthetase monodehydroascorbate reductase STAAAAS/ 3-isopropylmalate dehydratase* ALAEXAR/ end-xyloglucan transferase AGFYEKF/ germin A SDPSPLQ/ alcohol dehydrogenase aminopeptidase N SAM-dependent methyltransferase* RLDVXGY/

- 23 - histidine ammonia-lyase* ISTGFXE/ dihydropyrimidine dehydrogenase SAGAGEP/ succunyl-CoA ligase beta-chain LNIHEYQ/ NAD-dependent aldehyde dehydrogenase DIGAPIX/ pyridoxal phosphate biosynthesis protein* XNLNFIA/ thiamine biosynthesis enzyme* AVLFAPL/ malate/lactate dehydrogenase* STAFALA/ geranylgeranyl pyrophosphate synthase* XPAETVL/ nucleoside diphosphate kinase AEMERTF/ 12-oxophytodienolic acid reductase VNQAAMP/ proteasome subunit alpha type 3 peptidylprolyl isomerase Cyp1 26S proteasome regulatory subunit 4 glutelin ubiquitin RP-20 kDa 20S proteasome subunit beta-2 TTIVGLV/ 20S proteasome subunit beta-5 mitochondrial processing peptidase ASTSVAQ/ luminal binding protein cysteine proteinase QKEEHYY/ disulfide-isomerase DnaJ-class molecular chaperone* SVKSVKL/ peptidylprolyl isomerase ATADPKE/ chaperonin 21 kDa ATVVSPK/ ribosomal protein L11 ribosomal protein L16* SFKLXNF/ ribosomal protein S11 AGGXNPS/

- 24 - eukaryotic translation initiation factor SAAXPAP/ peptide chain release factor* SDPYDQN/ En/Spm-like transposon protein RGGKLVR/ transposase-related HPIFIKN/ glycine-rich RNA-binding protein 2 STKLFVG/ MADS4 box protein putative Dof zinc finger protein SGGGGGG/ transcription factor APF1 nuclear protein ABC transporter homolog DVTRLEX/ mitochondrial carrier protein MKTEYIV/ Rhicadhesin receptor GDPDMLQ/ secretory peroxidase root specific pathogenesis-related protein 10 cationic peroxidase peroxidase water steress inducible protein secretory peroxidase secretory peroxidase GTP-binding protein RAB5B GTP-binding protein zinc-binding peroxisomal integral membrane protein notchless protein homolog chlorophyll a/b-binding protein NADPH-thioredoxin reductase

- 25 - chlorophyll a-b binding protein 2 chlorophyll b synthase pyruvate dehydrogenase kinase glutathione reductase glucose-1-phosphate adenyltransferase small subunit inorganic pyrophosphatase plastidic ATP sulfurylase beta-1,3-glucanase L-ascorbate oxidase 3-hydroxy-3-methylglutaryl-coenzyme A reductase alpha 1,4-glucan phosphorylase H isozyme hexokinase I

Protein identificationa) N-terminalb) c) sequence L1 L2 L4 L8 L10 S2 S4 S8 ATP-dependent RNA helicase dTDP-glucose 4-6-dehydratase cyanate hydratase secretory acid phosphatase tryptophan synthase alpha chain hydroxylase alcohol dehydrogenase germin protein cytidine deaminase ribose 5-phosphate isomerase A

- 26 - aconitate hydratase 26S proteasome regulatory pqrticle triple-A ATPase subunit 5a mannose-binding rice lectin 26S proteasome regulatory particle non-ATPase subunit 12 26S proteasome regulatory subunit 7 40S ribosomal protein S20 ribosomal protein L18, cytoplasmic polyketide synthase zinc finger transcription factor DNA binding protein cyclin H-1 growth inhibitory protein chitinase alcohol dehydrogenase 2 peroxidase protein kinase acetylglutamate kinase nucleotide-binding protein TGF-beta receptor-interacting protein cssein-kinase JUN-activation-domain-binding protein NEPI-interacting protein feredoxin-NADP reductase isocitrate/isopropylmalate dehydrogenase dihydroflavonal-4-reductase adenylate kinase-b zeta-carotene desatulase

- 27 - pyrrolidone carboxyl peptidase lipoxygenase succinate dehydrogenase flavoprotein alpha subunit

NAD malate dehydrogenase 26S proteasome regulatory particle triple-A ATPase subunit 1b serine carboxypeptidase 50S ribosomal protein L5 ribosomal protein S10p/S20e ribiosomal protein bZIP transcription factor zinc finger protein typical P-type R2R3 myb protein FtsJ cell division protein gibberellin-insensitive protein OsGAI disease resistance response protein GTP-binding proteinm glyoxalase II pyruvate dehydrogenase kinase monodehydroascorbate reductase monoterpene synthase RNA helicase a) An asteric (*) stands for protein identified from sources other than plants b) X stands for unidntified amino acid c) X denotes the presence of observed proteinX

- 28 - d) Calculated value of Mr of protein which N-terminal sequence is determined is recalculated (see text). e) Accession number is cited from NCBI database.

- 29 -