58Th Annual Conference
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Proceedings of the 58th Annual Conference Rhode Island Convention Center Providence Rhode Island October 22-28, 2015 AAVLD Strategic Plan Adopted July 31, 2014 Vision The AAVLD is a world leader in advancing the discipline of veterinary diagnostic laboratory science to promote global animal health and One Health. Mission The AAVLD promotes continuous improvement and public awareness of veterinary diagnostic laboratories by advancing the discipline of veterinary diagnostic laboratory science. The AAVLD provides avenues for education, communication, peer-reviewed publication, collaboration, outreach, and laboratory accreditation. Motto: Advancing veterinary diagnostic laboratory science Core values The AAVLD is committed to these core values: • Continuous improvement • Engagement of members • Effective communication • Collaboration • Support of One Health Goals 1. Advocate for the role of veterinary diagnostic laboratories in global health. 2. Foster the continuous improvement of diagnostic laboratory techniques and processes, personnel qualifications, and facilities. 3. Promote the continued professional growth of members. 4. Disseminate information concerning the diagnosis/monitoring of animal health and disease surveillance. 5. Provide a formal accreditation process for veterinary medical diagnostic laboratories. American Association of Veterinary Laboratory Diagnosticians The American Association of Veterinary Laboratory Diagnosticians (AAVLD) is a not-for- profit professional organization which seeks to: • Disseminate information relating to the diagnosis of animal diseases. • Coordinate diagnostic activities of regulatory, research and service laboratories. • Establish uniform diagnostic techniques. • Improve existing diagnostic techniques. • Develop new diagnostic techniques. • Establish accepted guidelines for the improvement of diagnostic laboratory organizations relative to personnel qualifications and facilities. • Act as a consultant to the United States Animal Health Association on uniform diagnostic criteria involved in regulatory animal disease programs. AAVLD Officers, 2015 Executive Director Jim Kistler, Navarre, FL President François Elvinger, Blacksburg, VA President-elect Tom Baldwin, Login, UT Vice-president Pat Halbur, Ames, IA Secretary-Treasurer John Adaska, Tulare, CA Immediate Past-President Catherine Barr, Amarillo, TX AAVLD Executive Board, 2015 Executive Director Jim Kistler, Navarre, FL President François Elvinger, Blacksburg, VA President-elect Tom Baldwin, Login, UT Vice-president Pat Halbur, Ames, IA Secretary-Treasurer John Adaska, Tulare, CA Northeast Sandra Bushmich, Storrs, CT North-central Jane Christopher-Hennings, Brookings, SD Northwest Jerry Heidel, Corvallis, OR Southeast Lanny Pace, Pearl, MS South-central Hemant Kashinath Naikare, Amarillo, TX Southwest Hana Van Campen, Fort Collins, CO Canada Provincial Estela Cornaglia, St. Hyacinthe, QC Canada Federal, ex-Officio Maria Perrone, Ottawa, ON NVSL, ex-Officio Beverly, Schmitt, Ames, IA AVMA, ex-Officio Christine Hoang, Shaumburg, IL AAVLD Secretary-Treasurer's Office PO Box 6396, Visalia, CA 93290 Phone 559-781-8900 Fax 559-781-8989 Email: [email protected] Acknowledgments The success of a meeting is a function of both presenters and attendees - a special thank you to all who present their data and findings, to all exhibitors and sponsors, and to everyone who attends our meeting. We would also like to give a special thank you to all of our invited speakers and moderators for the AAVLD Plenary Session and the USAHA-AAVLD Plenary Session. Program Committee members, listed below, deserve special acknowledgement for their hard work, organization, review and editing of the abstracts, as do moderators of our scientific sessions. Kim Grant, from Thomson Reuters, helped us navigate the ScholarOne software. Jackie Cassarly coordinated all meeting room and exhibitor booth arrangements, sponsor agreements, breaks, and all of the many other details that go into making this meeting a success. ************************************************* Program Committee Scientific Session Moderators François Elvinger, Chair Kelli Almes Tom Baldwin, Co-chair Tom Baldwin Kelly Almes Karyn Bischoff Tim Baszler Dubraska V. Diaz-Campos Beate Crossley Tim Evans Santiago Diab Chanran Ganta Tim Frana Pat Halbur Ben Hause Kenitra Hammac Kevin Lahmers Gregg Hanzlicek Kristy Pabilonia Jamie Henningson Lalitha Peddireddi Lindsey Holmstrom Julia Ridpath E. Jane Kelly Susan Schommer Pat Lukens Durda Slavic Douglas Marthaler Patricia Talcott Christie Mayo Larry Thompson Danielle Nelson Jianqiang Zhang Devi Patnayak Lalitha Peddireddi Akhilesh Ramachandran Julia Ridpath Tricia Talcott Larry Thompson David Wilson Jianqiang Zhang ************************************************* Please note: Abstracts published in these proceedings were peer reviewed by the members of the Program Committee for data supporting conclusions to be presented, and were edited into a consistent format. Full manuscripts were not evaluated and readers should contact the author for referral to a full presentation of data or for permission to use, copy, or distribute data contained in an abstract. 2015 Trainee Travel Awardees Erica Noland Michigan State University Lorelei Clarke University of Georgia Jenny Pope University of Tennessee Pathology Committee Travel Awardee Shahan Azeem Iowa State University Nandita Mirajkar University of Minnesota Jennifer Dill University of Georgia Elizabeth Elsmo University of Georgia Courtney Smith Texas A&M University Boying Liang University of Kentucky Shubhada Chothe Pennsylvania State University Oi Chen Iowa State University Camila Abreu Federal University of Lavras 2015 Staff Travel Awardees Susan Martin University of Missouri Marc Schwabenlander University of Minnesota Emily Collin Kansas State University John Loy University of Nebraska-Lincoln Eric Lee Cornell University Daniel Corrales Texas A&M University 2015 ACVP/AAVLD Award Courtney Schott University of Guelph Table of Contents AAVLD Plenary Session Saturday, October 24, 2015 Ballroom D-E Moderators: Tom Baldwin and Pat Halbur 7:45 AM Introduction and Welcome 7:50 AM The 2015 avian influenza outbreak: phylogenic analysis of the H5N2 influenza virus Mia Kim Torchetti . 27 8:30 AM The University of Minnesota Veterinary Diagnostic Laboratory: Responding to Highly Pathogenic Avian Influenza Ann M . Fitzpatrick, Mary Thurn, Elizabeth Wiedenman, Sunil Kumar, Robert Porter, Kurt D . Rossow . 28 9:00 AM Second verse, but not quite the same as the first: Tips on preparing your lab for HPAIV ◊ Rodger Main, Sarah Abate, David Baum, Phillip Gauger, Patrick G . Halbur, Karen Harmon, Yuko Sato, Wendy R . Stensland, Katie Woodard, Kyoung-Jin Yoon, Jianqiang Zhang . 29 9:30 AM Break 10:15 AM Highly Pathogenic Avian Influenza Outbreak: epidemiologic findings and what they tell us about prevention and control Brian McCluskey . .. 30 10:55 AM Avian influenza outbreak of 2015: regulatory effort and what was learned John R . Clifford . 31 11:35 AM Concluding Remarks and Questions Symbols at the end of titles indicate the following designations: § AAVLD Laboratory Staff Travel Awardee * Graduate Student Poster Presentation Award Applicant # AAVLD Trainee Travel Awardee † Graduate Student Oral Presentation Award Applicant + AAVLD/ACVP Pathology Award Applicant ◊ USAHA Paper AAVLD Annual Conference Proceedings 1 Providence, RI October 2015 Bacteriology 1 Saturday, October 24, 2015 556 Moderators: Lalitha Peddireddi and Kenitra Hammac 1:00 PM Avian Coxiella-like infection in a hyacinth macaw † + Melissa Macias Rioseco, Margaret A . Highland, Rocio Crespo . 35 1:15 PM Streptococcus bovis Septicemia in a Group of Calves following Intramuscular Vaccination # † + Lorelei L . Clarke, Robert L . Fathke, Susan Sanchez, James B . Stanton . 36 1:30 PM A multiplex real-time PCR panel for detection of major bacterial and viral agents causing abortion in small ruminants # † Shahan Azeem, Sara Ghorbani-Nezami, Ahmad Allam, Vickie L Cooper, Eric Burrough, Kent Schwartz, Paul Plummer, Paulo Arruda, Phillip Gauger, Joann M . Kinyon, Darin Madson, Orhan Sahin, Gregory Stevenson, Kyoung-Jin Yoon . 37 1:45 PM Genetic characterization of novel pathogen “Brachyspira hampsonii” reveals associations of diverse clades, regions, host species and relationships with other swine Brachyspira species # † Nandita Mirajkar, Aschalew Bekele, Yogesh Chander, Connie Gebhart . 38 2:00 PM A diagnostic tool for the veterinary clinician and pathologist: panfungal polymerase chain reaction (PCR) on formalin-fixed paraffin embedded (FFPE) tissues to classify fungal organisms found histopathologically # † Courtney M . Smith, Caitlin Older, Laura Bryan, Sara Lawhon, Jan Suchodolski, Joanne Mansell, Aline Rodrigues-Hoffmann . 39 2:15 PM Application of MIQE guidelines for quantitative detection of Lawsonia intracellularis by PCR Kent Doolittle, Wayne Chittick, Allison Spitz, Greg Cline, Jack Gallup . 40 2:30 PM Comparative Assessment of Multiplex PCR Assays to Culture and Susceptibility Testing Methods for the Detection of Bovine Bacterial Respiratory Pathogens and Macrolide Antimicrobial Resistance Determinants § Laura Pike, John D . Loy . 41 2:45 PM Clinical evaluation of Vetericyn Plus™ Pinkeye Spray in calves with experimentally induced Infectious Bovine Keratoconjunctivitis ◊ Julie Gard, Debra R . Taylor, Rachel Maloney, Megan Schnuelle, Sue Duran, Will Justus, Paul Walz, Annette O’Connor . 42 Symbols at the end of titles indicate the following designations: