2015: Year of the Frog
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Lora Snake (Philodryas Olfersii) Venom
Revista da Sociedade Brasileira de Medicina Tropical 39(2):193-197, mar-abr, 2006 ARTIGO/ARTICLE Experimental ophitoxemia produced by the opisthoglyphous lora snake (Philodryas olfersii) venom Ofitoxemia experimental produzida pelo veneno da serpente opistoglifa lora (Philodryas olfersii) Alexis Rodríguez-Acosta1, Karel Lemoine1, Luis Navarrete1, María E. Girón1 and Irma Aguilar1 ABSTRACT Several colubrid snakes produce venomous oral secretions. In this work, the venom collected from Venezuelan opisthoglyphous (rear-fanged) Philodryas olfersii snake was studied. Different proteins were present in its venom and they were characterized by 20% SDS-PAGE protein electrophoresis. The secretion exhibited proteolytic (gelatinase) activity, which was partially purified on a chromatography ionic exchange mono Q2 column. Additionally, the haemorrhagic activity of Philodryas olfersii venom on chicken embryos, mouse skin and peritoneum was demonstrated. Neurotoxic symptoms were demonstrated in mice inoculated with Philodryas olfersii venom. In conclusion, Philodryas olfersii venom showed proteolytic, haemorrhagic, and neurotoxic activities, thus increasing the interest in the high toxic action of Philodryas venom. Key-words: Colubridae. Haemorrhage. Neurotoxic. Philodryas olfersii. Proteolytic activity. Venom. RESUMO Várias serpentes da família Colubridae produzem secreções orais venenosas. Neste trabalho, foi estudado o veneno coletado da presa posterior da serpente opistóglifa venezuelana Philodryas olfersii. Deferentes proteínas estavam presentes no veneno, sendo caracterizadas pela eletroforese de proteínas (SDS-PAGE) a 20%. A secreção mostrou atividade proteolítica (gelatinase) a qual foi parcialmente purificada em uma coluna de intercâmbio iônico (mono Q2). Adicionalmente, a atividade hemorrágica do veneno de Philodryas olfersii foi demonstrada em embriões de galinha, pele e peritônio de rato. Os sintomas neurológicos foram demonstrados em camundongos inoculados com veneno de Philodryas olfersii. -
Bites by the Colubrid Snake Philodryas Patagoniensis: a Clinical and Epidemiological Study of 297 Cases
Toxicon 56 (2010) 1018–1024 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon Bites by the colubrid snake Philodryas patagoniensis: A clinical and epidemiological study of 297 cases Carlos R. de Medeiros a,b,*, Priscila L. Hess c, Alessandra F. Nicoleti d, Leticia R. Sueiro e, Marcelo R. Duarte f, Selma M. de Almeida-Santos e, Francisco O.S. França a,d a Hospital Vital Brazil, Instituto Butantan, Av. Vital Brazil 1500, 05503-900, São Paulo, SP, Brazil b Serviço de Imunologia Clínica e Alergia, Departamento de Clínica Médica, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil c Laboratório de Imunoquímica, Instituto Butantan, São Paulo, SP, Brazil d Departamento de Moléstias Infecciosas e Parasitárias, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil e Laboratório de Ecologia e Evolução, Instituto Butantan, São Paulo, SP, Brazil f Laboratório de Herpetologia, Instituto Butantan, São Paulo, SP, Brazil article info abstract Article history: We retrospectively analyzed 297 proven cases of Philodryas patagoniensis bites admitted to Received 9 November 2009 Hospital Vital Brazil (HVB), Butantan Institute, São Paulo, Brazil, between 1959 and 2008. Received in revised form 4 July 2010 Only cases in which the causative animal was brought and identified were included. Part of Accepted 9 July 2010 the snakes brought by the patients was still preserved in the collection maintained by the Available online 17 July 2010 Laboratory of Herpetology. Of the 297 cases, in 199 it was possible to describe the gender of the snake, and seventy three (61.3%) of them were female. -
(Wiegmann, 1835) (Reptilia, Squamata, Dipsadidae) from Chile
Herpetozoa 32: 203–209 (2019) DOI 10.3897/herpetozoa.32.e36705 Observations on reproduction in captivity of the endemic long-tailed snake Philodryas chamissonis (Wiegmann, 1835) (Reptilia, Squamata, Dipsadidae) from Chile Osvaldo Cabeza1, Eugenio Vargas1, Carolina Ibarra1, Félix A. Urra2,3 1 Zoológico Nacional, Pio Nono 450, Recoleta, Santiago, Chile 2 Programa de Farmacología Molecular y Clínica, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Chile 3 Network for Snake Venom Research and Drug Discovery, Santiago, Chile http://zoobank.org/8167B841-8349-41A1-A5F0-D25D1350D461 Corresponding author: Osvaldo Cabeza ([email protected]); Félix A. Urra ([email protected]) Academic editor: Silke Schweiger ♦ Received 2 June 2019 ♦ Accepted 29 August 2019 ♦ Published 10 September 2019 Abstract The long-tailed snake Philodryas chamissonis is an oviparous rear-fanged species endemic to Chile, whose reproductive biology is currently based on anecdotic reports. The characteristics of the eggs, incubation time, and hatching are still unknown. This work describes for the first time the oviposition of 16 eggs by a female in captivity at Zoológico Nacional in Chile. After an incubation period of 59 days, seven neonates were born. We recorded data of biometry and ecdysis of these neonates for 9 months. In addition, a review about parameters of egg incubation and hatching for Philodryas species is provided. Key Words Chile, colubrids, eggs, hatching, oviposition, rear-fanged snake, reproduction Introduction Philodryas is a genus composed of twenty-three ovipa- especially P. aestiva (Fowler and Salomão 1995; Fowl- rous species widely distributed in South America (Grazzi- er et al. 1998), P. nattereri (Fowler and Salomão 1995; otinet al. -
Abstracts Part 1
375 Poster Session I, Event Center – The Snowbird Center, Friday 26 July 2019 Maria Sabando1, Yannis Papastamatiou1, Guillaume Rieucau2, Darcy Bradley3, Jennifer Caselle3 1Florida International University, Miami, FL, USA, 2Louisiana Universities Marine Consortium, Chauvin, LA, USA, 3University of California, Santa Barbara, Santa Barbara, CA, USA Reef Shark Behavioral Interactions are Habitat Specific Dominance hierarchies and competitive behaviors have been studied in several species of animals that includes mammals, birds, amphibians, and fish. Competition and distribution model predictions vary based on dominance hierarchies, but most assume differences in dominance are constant across habitats. More recent evidence suggests dominance and competitive advantages may vary based on habitat. We quantified dominance interactions between two species of sharks Carcharhinus amblyrhynchos and Carcharhinus melanopterus, across two different habitats, fore reef and back reef, at a remote Pacific atoll. We used Baited Remote Underwater Video (BRUV) to observe dominance behaviors and quantified the number of aggressive interactions or bites to the BRUVs from either species, both separately and in the presence of one another. Blacktip reef sharks were the most abundant species in either habitat, and there was significant negative correlation between their relative abundance, bites on BRUVs, and the number of grey reef sharks. Although this trend was found in both habitats, the decline in blacktip abundance with grey reef shark presence was far more pronounced in fore reef habitats. We show that the presence of one shark species may limit the feeding opportunities of another, but the extent of this relationship is habitat specific. Future competition models should consider habitat-specific dominance or competitive interactions. -
On the Trail N°26
The defaunation bulletin Quarterly information and analysis report on animal poaching and smuggling n°26. Events from the 1st July to the 30th September, 2019 Published on April 30, 2020 Original version in French 1 On the Trail n°26. Robin des Bois Carried out by Robin des Bois (Robin Hood) with the support of the Brigitte Bardot Foundation, the Franz Weber Foundation and of the Ministry of Ecological and Solidarity Transition, France reconnue d’utilité publique 28, rue Vineuse - 75116 Paris Tél : 01 45 05 14 60 www.fondationbrigittebardot.fr “On the Trail“, the defaunation magazine, aims to get out of the drip of daily news to draw up every three months an organized and analyzed survey of poaching, smuggling and worldwide market of animal species protected by national laws and international conventions. “ On the Trail “ highlights the new weapons of plunderers, the new modus operandi of smugglers, rumours intended to attract humans consumers of animals and their by-products.“ On the Trail “ gathers and disseminates feedback from institutions, individuals and NGOs that fight against poaching and smuggling. End to end, the “ On the Trail “ are the biological, social, ethnological, police, customs, legal and financial chronicle of poaching and other conflicts between humanity and animality. Previous issues in English http://www.robindesbois.org/en/a-la-trace-bulletin-dinformation-et-danalyses-sur-le-braconnage-et-la-contrebande/ Previous issues in French http://www.robindesbois.org/a-la-trace-bulletin-dinformation-et-danalyses-sur-le-braconnage-et-la-contrebande/ -
(Leptophis Ahaetulla Marginatus): Characterization of Its Venom and Venom-Delivery System
(This is a sample cover image for this issue. The actual cover is not yet available at this time.) This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the author's institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/authorsrights Author's Personal Copy Toxicon 148 (2018) 202e212 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon Assessment of the potential toxicological hazard of the Green Parrot Snake (Leptophis ahaetulla marginatus): Characterization of its venom and venom-delivery system Matías N. Sanchez a, b, Gladys P. Teibler c, Carlos A. Lopez b, Stephen P. Mackessy d, * María E. Peichoto a, b, a Consejo Nacional de Investigaciones Científicas y Tecnicas (CONICET), Ministerio de Ciencia Tecnología e Innovacion Productiva, Argentina b Instituto Nacional de Medicina Tropical (INMeT), Ministerio de Salud de la Nacion, Neuquen y Jujuy s/n, 3370, Puerto Iguazú, Argentina c Facultad de Ciencias Veterinarias (FCV), -
Colubrid Venom Composition: an -Omics Perspective
toxins Review Colubrid Venom Composition: An -Omics Perspective Inácio L. M. Junqueira-de-Azevedo 1,*, Pollyanna F. Campos 1, Ana T. C. Ching 2 and Stephen P. Mackessy 3 1 Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo 05503-900, Brazil; [email protected] 2 Laboratório de Imunoquímica, Instituto Butantan, São Paulo 05503-900, Brazil; [email protected] 3 School of Biological Sciences, University of Northern Colorado, Greeley, CO 80639-0017, USA; [email protected] * Correspondence: [email protected]; Tel.: +55-11-2627-9731 Academic Editor: Bryan Fry Received: 7 June 2016; Accepted: 8 July 2016; Published: 23 July 2016 Abstract: Snake venoms have been subjected to increasingly sensitive analyses for well over 100 years, but most research has been restricted to front-fanged snakes, which actually represent a relatively small proportion of extant species of advanced snakes. Because rear-fanged snakes are a diverse and distinct radiation of the advanced snakes, understanding venom composition among “colubrids” is critical to understanding the evolution of venom among snakes. Here we review the state of knowledge concerning rear-fanged snake venom composition, emphasizing those toxins for which protein or transcript sequences are available. We have also added new transcriptome-based data on venoms of three species of rear-fanged snakes. Based on this compilation, it is apparent that several components, including cysteine-rich secretory proteins (CRiSPs), C-type lectins (CTLs), CTLs-like proteins and snake venom metalloproteinases (SVMPs), are broadly distributed among “colubrid” venoms, while others, notably three-finger toxins (3FTxs), appear nearly restricted to the Colubridae (sensu stricto). -
Helminths Infecting the Black False Boa Pseudoboa Nigra(Squamata
Acta Herpetologica 13(2): 171-175, 2018 DOI: 10.13128/Acta_Herpetol-23366 Helminths infecting the black false boa Pseudoboa nigra (Squamata: Dipsadidae) in northeastern Brazil Cicera Silvilene L. Matias1,*, Cristiana Ferreira-Silva2, José Guilherme G. Sousa3, Robson W. Ávila1,3 1 Laboratório de Herpetologia, Departamento de Química Biológica, Universidade Regional do Cariri, Campus do Pimenta, CEP 63105000, Crato, CE, Brazil. *Corresponding author. E-mail: [email protected] 2 Programa de Pós-Graduação em Ciências Biológicas (Zoologia), Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, CEP 18080-970, Botucatu, SP, Brazil 3 Programa de Pós-Graduação em Ecologia e Recursos Naturais, Departamento de Ciências Biológicas, Universidade Federal do Ceará, Campus Universitário do Pici, CEP 60021970 Fortaleza, CE, Brazil Submitted on: 2018, 8th June; revised on: 2018, 28th August; accepted on: 2018, 13th September Editor: Daniele Pellitteri-Rosa Abstract. Knowledge about endoparasites of snakes is essential to understand the ecology of both parasites and hosts. Herein, we present information on helminths parasitizing the black false boa Pseudoboa nigra in northeastern Bra- zil. We examined 32 specimens from five Brazilian states (Ceará, Piauí, Pernambuco, Maranhão and Rio Grande do Norte). We found six helminths taxa: two acanthocephalans (Acanthocephalus sp. and Oligacanthorhychus sp.), three nematodes (Hexametra boddaertii, Physaloptera sp. and Physalopteroides venancioi), and one cestode (Ophiotaenia sp.). All parasites are reported for the first time infecting P. nig ra , providing relevant information on infection patterns in this snake. Keywords. Acanthocephala, Cestoda, Nematoda, Reptilia, snake. Surveys of endoparasites associated with wild ani- 2015). However, little is known about infection patterns mals are key features to understand ecology, natural his- in snakes from Brazil (Almeida et al., 2008). -
Molecular Phylogeny of the Tribe Philodryadini Cope, 1886 (Dipsadidae: Xenodontinae): Rediscovering the Diversity of the South American Racers
ARTICLE Molecular phylogeny of the tribe Philodryadini Cope, 1886 (Dipsadidae: Xenodontinae): Rediscovering the diversity of the South American Racers Juan Camilo Arredondo¹⁶; Felipe G. Grazziotin²; Gustavo J. Scrocchi³; Miguel Trefaut Rodrigues⁴; Sandro Luís Bonatto⁵ & Hussam Zaher⁶⁷ ¹ Universidad CES, Facultad de Ciencias y Biotecnología, Colecciones Biológicas Universidad CES (CBUCES). Medellín, Antioquia, Colombia. ORCID: http://orcid.org/0000-0003-1925-4556. E-mail: [email protected] ² Instituto Butantan, Laboratório Especial de Coleções Zoológicas (LECZ). São Paulo, SP, Brasil. ORCID: http://orcid.org/0000-0001-9896-9722. E-mail: [email protected] ³ Fundación Miguel Lillo, Unidad Ejecutora Lillo (CONICET-UEL). San Miguel de Tucumán, Tucumán, Argentina. ORCID: http://orcid.org/0000-0002-8924-8808. E-mail: [email protected] ⁴ Universidade de São Paulo (USP), Instituto de Biociências (IB-USP), Departamento de Zoologia. São Paulo, SP, Brasil. ORCID: http://orcid.org/0000-0003-3958-9919. E-mail: [email protected] ⁵ Pontifícia Universidade Católica do Rio Grande do Sul (PUC-RS), Escola de Ciências da Saúde e da Vida, Laboratório de Biologia Genômica e Molecular. ORCID: http://orcid.org/0000-0002-0064-467X. E-mail: [email protected] ⁶ Universidade de São Paulo (USP), Museu de Zoologia (MZUSP). São Paulo, SP, Brasil. ⁷ ORCID: http://orcid.org/0000-0002-6994-489X. E-mail: [email protected] (corresponding author) Abstract. South American racers of the tribe Philodryadini are a widespread and diverse group of Neotropical snakes with a complex taxonomic and systematic history. Recent studies failed to present a robust phylogenetic hypothesis for the tribe, mainly due to incomplete taxon sampling. Here we provide the most extensive molecular phylogenetic analysis of Philodryadini available so far, including 20 species (83% of the known diversity) from which six were not sampled previously. -
Distribution, Diversity and Conservation Status of Bolivian Reptiles
Distribution, diversity and conservation status of Bolivian Reptiles Dissertation zur Erlangung des Doktorgrades (Dr. rer. nat.) der Mathematisch-Naturwissenschaftlichen Fakultät der Rheinischen Friedrichs-Wilhelms-Universität Bonn vorgelegt von Dirk Embert aus Bonn Bonn, 2007 Erstgutachter: Prof. Dr. Wolfgang Böhme Zweitgutachter: Prof. Dr. Gerhard Kneitz Mündliche Prüfung: 29.11.2007 Diese Dissertation ist auf dem Hochschulschriftenserver der ULB Bonn http://hss.ulb.uni-bonn.de/diss_online elektronisch publiziert. 2008 Dedication This work is dedicated to my grandparents Franz and Irene Roeder. Their support and love they shared with me throughout all my life will always be in my heart. Widmung Diese Arbeit widme ich meinen Großeltern Franz und Irene Roeder. Ihre Unterstuetzung und Liebe die sie mir gegeben haben werden immer einen Platz in meinem Herzen haben. Abstract The study area was defined as being the whole country of Bolivia. The Conservation Status of Bolivian Reptiles has been poorly investigated. Very few species had been assessed by the IUCN and very few were listed in CITES. As Bolivia still is within the countries with best conserved habitat, now is the moment to plan the conservation of its Biodiversity. This makes the present study urgent and necessary. To be able to identify the conservation status of the reptiles of Bolivia first the species had to be identified correctly, a complete list of reptiles in Bolivia, and a most complete possible database had to be elaborated including geo-referenced data. On base of the obtained information distribution of the species had been extrapolated with the Distribution Model BIOM (Sommer et. al 2002). Later on the maps were overlaid to get different maps as species richness and endemism richness. -
HERPETOLOGICAL HUSBANDRY Soil and Laid a Second Egg on 17 May
Female #1 tunneled from under the shelter to the bottom of the HERPETOLOGICAL HUSBANDRY soil and laid a second egg on 17 May. The first egg laid by this female remained on the soil surface and failed to hatch. Female #2 also dug a tunnel and laid three eggs on 18 May. Neither female Notes on the Captive Reproductive Behavior of accepted mealworms or crickets offered as food. the Asiatic Four-lined Skink, Observations of both females and their eggs were made through the transparent floor of the cages, or by carefully removing the Eumeces quadrilineatus shelters. Both females used their bodies to surround their eggs throughout incubation. Although they occasionally shifted their AKIKO HOSONO position within the tunnels, they remained under the soil and never and appeared on the surface until their eggs hatched (see below). TSUTOMU HIKIDA When Female #1 was observed outside her tunnel on 27 June, Department of Zoology, Graduate School of Science, Kyoto University, we examined her egg and found a hatchling (Hatchling #1). Female Kitashirakawa, Sakyo, Kyoto 606-8502 Japan #2 emerged from the soil on 8 July, whereupon we excavated e-mail (AH): [email protected] three hatchlings (Hatchlings #2, 3, and 4), while Hatchling #4 Eumeces quadrilineatus ranges through southern China, was just struggling to extricate itself from its egg. Assuming that Vietnam, Cambodia, and Thailand (Taylor 1935). It has been the females emerged upon the hatching of their eggs, as is the grouped with the American four-lined forms (E. gilberti, E. case of other congeneric species (e.g., E. -
Phylogenetic Systematics and Taxonomic Review of the Snakes of the Tribe Philodryadini Cope, 1886 (Dipsadidae: Xenodontinae)
Juan Camilo Arredondo Salgar Phylogenetic systematics and taxonomic review of the snakes of the tribe Philodryadini Cope, 1886 (Dipsadidae: Xenodontinae) Sistemática filogenética e revisão taxonômica das serpentes da tribo Philodryadini Cope, 1886 (Dipsadidae: Xenodontinae) Corrected Version Thesis presented to the Post-Graduate Program of the Museu de Zoologia da Universidade de São Paulo to obtain the degree of Doctor of Science (Systematics, Animal Taxonomy and Biodiversity). Advisor: Prof. Hussam El Dine Zaher, PhD São Paulo 2019 Abstract The tribe Philodryadini is constituted by a rich group of neotropical snakes that are highly diverse ecologically and morphologically. Currently, 24 species compose the tribe, and are recognized as common components of the ophidian diversity in several regions of South America. The species of Philodryadini exhibit two great geographical distribution patterns, with most species occurring in the lowlands of the cis-Andean region of the American continent, while another not so diverse group is distributed in the trans-Andean region of the central and southern Andes, in Ecuador, Peru and Chile. The richness of the tribe and its evolutionary relationships has varied greatly in recent years, mainly due to the recent formulation of diverse phylogenetic hypotheses based on molecular evidence. In the same way, in recent years many taxonomic complexes have been studied and the taxonomic status of several species has been clarified. However, many questions about the status of some complexes and phylogenetic relationships within the tribe are still unknown. To understand the evolutionary relationships between Philodryadini and the other Xenodontinae tribes we performed a phylogenetic analysis including molecular evidence of a representative sample of all tribes of the subfamily.