Product datasheet Anti-NOX5 Antibody Catalog Number: A03119

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-NOX5 Antibody Name NOX5 Source Rabbit IgG Species Reactivity human Tested Application WB,IHC-P,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human NOX5 recombinant (Position: A21-F765). Purification Immunogen affinity purified. Observed MW 86KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT- PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NOX5 Antibody Catalog Number: A03119

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2.IHC analysis analysis of anti- NOX5 using anti- NOX5 antibody (A03119).The antibody (A03119). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human pancreatic cancer conditions.Lane 1: human tissue. Biotinylated goat HEK293 whole cell anti-rabbit IgG was used lysates,Lane 2: human as secondary antibody. Jurkat whole cell The tissue section was lysates,Use rabbit anti- developed using Strepavi NOX5 1:1000, probed din-Biotin-Complex with a goat anti-rabbit (SABC) (Catalog # IgG-HRP secondary SA1022) with DAB as the antibody. The signal is chromogen. developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NOX5 at approximately 86KD. The expected band size for NOX5 is at 86KD.

Figure 3.IHC analysis Figure 4.IHC analysis using anti- NOX5 using anti- NOX5 antibody (A03119). antibody (A03119). detected in paraffin- detected in paraffin- embedded section of embedded section of rat mouse spleen tissue. spleen tissue. Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody. The secondary antibody. The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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