NADPH oxidase 5 (h.NOX5)

Catalog number 144945 Supplier United States Biological

NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a which in humans is encoded by the NOX5 . The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al. (2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al. (2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells. UniProt Number Q96PH1 Gene ID NOX5 Applications Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Handling Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human NOX5. Formulation Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Purity Purified by immunoaffinity chromatography. Specificity Recognizes human h.NOX5. No crossreactivity with other . Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications IC IHC WB Crossreactivity Hu Storage 4°C/-20°C Reference Ca2+-activated NADPH oxidase in testis, spleen, and lymph nodes. J. Biol. Chem. 276: 37594-37601, 2001. 2. Kamiguti, A. S., Serrander, L., Lin, K., Harris, R. J., Cawley, J. C., Allsup, D. J., Slupsky, J. R., Krause, K.-H., Zuzel, M. Expression and activity of NOX5 in the circulating malignant B cells of hairy cell leukemia. J. Immun. 175: 8424-8430, 2005.

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