Recombinant Mouse GDF-7/BMP-12 Catalog Number: 779-G7/CF

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Recombinant Mouse GDF-7/BMP-12 Catalog Number: 779-G7/CF DESCRIPTION Source E. coliderived Thr308Arg453 Accession # NP_038555 Nterminal Sequence Thr308 Analysis Structure / Form Disulfidelinked homodimer Predicted Molecular 15 kDa (monomer) Mass SPECIFICATIONS SDSPAGE 28 kDa, nonreducing conditions Activity Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.251.25 µg/mL. Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method. Purity >95%, by SDSPAGE under reducing conditions and visualized by silver stain. Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details. PREPARATION AND STORAGE Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 20 to 70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 3 months, 20 to 70 °C under sterile conditions after reconstitution. BACKGROUND Growth Differentiation Factor 7 (GDF7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor β (TGFβ) superfamily. Mature GDF7 contains 146 amino acid (aa) residues with the characteristic seven conserved cysteine residues. The bioactive GDF7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF5, GDF6 and GDF7, which share 80 86% identity with each other, define a new subgroup within the BMP family. Like other TGFβ superfamily proteins, GDF7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF7 are 98% identical. It has been reported that GDF7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF7 response is mediated by the formation of heterooligomeric complexes of type I (BMPRIB) and type II (BMPRII or Activin RII) sereine/threonine kinase receptors, and the activiation of Smad proteins. References: 1. Storm, E.E., et al. (1994) Nature 368:639. 2. Nishitoh, H., et al. (1996) J. Biol. Chem. 271:21345. 3. FrancisWest, P.H., et al. (1999) Development 126:1035. 4. Massague, J. et al. (2000) Genes and Dev. 14:627. Rev. 2/6/2018 Page 1 of 1 .

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Gene Expressional Differences in MCF-7 vs. MCF-10A cells, Venn Groups e k g b Smad target genes (48) l o n i a h m j d Transcription Factors, And Genes Regulating Developmental Processes (44) f c Adhesion and TGF Superfamily Extracellular Molecules Cytokines (27) (44) Minimum Fold expression values ->640, -320, -160, -80, -40, -20, -10, -5, -2.5, 0, 2.5, 5, 10, 20, 40, 80, 160, 320, 640< + Supplemental Fig. 1 Supplemental Table 1. Change of Basal Gene Expressional values in MCF-7 as compared to MCF-10A cell line. Fold T-test Gene # GeneBank Symbol Up/Down p-Value Description /Position Regulation Venn Group a 01 /A01 NM_001105 ACVR1 -4.96 0.0000 Activin A receptor, type I 02 /A02 NM_001616 ACVR2A -2.62 0.0007 Activin A receptor, type IIA 03 /A03 NM_000020 ACVRL1 -1.25 0.7013 Activin A receptor type II-like 1 05 /A05 NM_020547 AMHR2 1.08 0.8043 Anti-Mullerian hormone receptor, type II 16 /B04 NM_004329 BMPR1A -2.40 0.0011 Bone morphogenetic protein receptor, type IA 17 /B05 NM_001203 BMPR1B -7.37 0.0000 Bone morphogenetic protein receptor, type IB 36 /C12 NM_000557 GDF5 -1.38 0.4911 Growth differentiation factor 5 (cartilage-derived morphogenetic protein-1) 37 /D01 NM_001001557 GDF6 1.12 0.9002 Growth differentiation factor 6 38 /D02 NM_182828 GDF7 -1.52 0.4995 Growth differentiation factor 7 53 /E05 NM_020997 LEFTY1 -1.76 0.0529 Left-right determination factor 1 59 /E11 NM_018055 NODAL -3.62 0.1290 Nodal homolog (mouse) 77 /G05 NM_003238 TGFB2 -4.52 0.0566 Transforming growth factor, beta 2 78 /G06 NM_003239 TGFB3 -1.12 0.2902 Transforming
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