Bioremediation of Groundwater: an Overview

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Bioremediation of Groundwater: an Overview International Journal of Applied Engineering Research ISSN 0973-4562 Volume 13, Number 24 (2018) pp. 16825-16832 © Research India Publications. http://www.ripublication.com Bioremediation of Groundwater: An Overview Shivam Mani Tripathi 1, Shri Ram2 1 ,2 Civil Engineering Department, MMMUT Gorakhpur, India Abstract can be treated on site, thus reducing exposure risks for clean- In past, we have large open area and abundant land resources up personnel, or potentially wider exposure as a result of and groundwater. But after the industrialization the use of transportation accidents. Methodology of this process is not hazardous chemicals increased due to unmanageable technically difficult, considerable experience and knowledge conditions. The chemical disposed on the ground surface & may require implementing this process, by thoroughly many other anthropogenic activities by humans like use of investigating the site and to know required condition to pesticides and oil spillage contaminated the soil and achieve. groundwater. The different type of contaminant by percolation The usual technique of remediation is to plow up through the ground reach to the aquifer and get affected which contaminated soil and take away to the site, or to cover or cap causes serious problem. We spend lot of money and use many the contaminated area. There are some drawbacks. The first technologies to extract and remediate the contamination. In method simply transport the contaminated materials which spite of different methods, bioremediation is a technology create major risks is excavation, handling, and transport of which is cost efficient and effective by using natural microbes hazardous material. It is very difficult to find the new landfill and degrades the contaminants the conditions and different sites for disposal. The cover or cap method is only temporary bioremediation technologies like in-situ, ex-situ, solution whereas contaminants remain on site which requires phytoremediation technology are described. Some monitoring and maintaining the engineered barrier for a long contaminates like LNAPLS, DNAPLS, BTEX, TCE, time which increase the cost and the liabilities. The superior Ammonia etc. also briefly described. The different sources, & and advanced methods than those previous methods which condition & technologies of remediation are also discussed. completely eliminate the pollutants or least transformation to innocuous substances. Some technologies that have been used INTRODUCTION are high-temperature incineration and various types of chemical decomposition (e.g., base-catalyzed de-chlorination, Water beneath the ground which saturates the pores of UV oxidation). They can be very effective at reducing levels subsurface is known as groundwater. Huge populations of of a range of contaminants, but have several drawbacks, world depend upon the groundwater as the source of their principally their technological complexity, the cost for small- livelihood. About 1.69 percent of total water present in ground scale application, and the lack of public acceptance, especially are highly mineralized groundwater they don’t require much for incineration that may increase the exposure to treatment before use. They get filtered and mineralized by contaminants for both the workers at the site and nearby their movement in ground. But after industrialization and residents. increase in anthropogenic activity of human, many industrial effluents percolate the ground and reach to the water table which contaminates the groundwater some chemicals like FACTORS OF BIO-REMEDIATION Methyl Tert-Butyl Ether (MTBE), Benzene, toluene, and the three xylene isomers (BTEX),etc. percolates to the Bio-remediation is a complex method to optimize this method groundwater and causes serious problem there are some heavy it requires several factors to work in appropriate manner metals like chromium, environmental factors such as pH, temperature, type of soil, presence of oxygen etc. These conditions required to cadmium, mercury, cobalt, lead, arsenic can cause various biological growth of micro-organism which treat the disease such as cancer, cardiovascular and neurological contamination is control manner. Some of the factors and diseases etc. for extraction or treatment of these contamination acceptable condition on which bioremediation work are world are using different techniques and investing million shown below in Table1: billions of money. Bioremediation is the one of the technique by which we can easily and feasibly extract or treat such type Table 1: Factors of Bioremediation of contamination. It is a process by which organic wastes are biologically degraded under controlled conditions to a mild Factors Required Condition state, or to levels below concentration limits established by Micro-organism Aerobic and Anaerobic regulatory authorities for bioremediation to be effective; Biological Process Catabolism and Anabolism microorganisms must enzymatically attack the pollutants and alter them to harmless products. Bioremediation can be Environmental Temperature, pH, Oxygen content, successful only where environmental conditions allow Factor Electron acceptor or donor microbial growth and activity, its purpose often includes the Nutrient Carbon, Nitrogen, Oxygen etc changes in environmental parameters to allow microbial Soil Moisture 25-28% of water holding capacity growth and degradation to proceed at a faster rate. Type of Soil Clay and silty soil Bioremediation techniques are typically more economical than other methods such as incineration, and some pollutants 16825 International Journal of Applied Engineering Research ISSN 0973-4562 Volume 13, Number 24 (2018) pp. 16825-16832 © Research India Publications. http://www.ripublication.com Micro-organism can be grown in almost in all environmental Low concentration of contaminants can be directly treated by conditions. Microbes will accumulates and grow at sub zero bioremediation. It is time taking process & it takes about 6 temperature as well as at very high temperature or in water month to a 1 year or more to purify soil containing 2 % of medium or in presence or in absence of oxygen it can be oils, but if the concentration is 0.8 or less percentage of heavy grown in presence of any hazardous compound or is waste oil present it purify within 1 to 2 months. These methods help stream. The presence of carbon source as the energy of in recycling and reuse the soil with some efforts it is a enzyme that can be used to remediate or degrade the environmentally friendly process. contaminants are required for microbial growth. We can subdivide these micro-organisms into following groups: Table 2: Environmental Condition Aerobic like Pseudomonas, Alcaligenes, Sphingomonas, Rhodococcus, and Mycobacterium. These Environmental Optimum Condition required microbes remediate the hydrocarbon and pesticides, both Factors Concentration for microbial alkanes and compounds. Lots of these bacteria are the main Activity source of energy and carbon it accumulate in presence of Soil Moisture Water holding 25-28% water oxygen. capacity 25-85% holding capacity Anaerobic are not much use as the aerobic bacteria. pH 6.5-8.0 5.5-8.5 It can be used to bio-remediate the polychlorinated biphenyls (PCBs) in river sediments, dechlorination of the solvent Nutrient C:N:P 120:10:1 N and P for growth Trichloroethylene (TCE), and chromium. It works in the of microbes absence of oxygen. Temperature 20-30°C 15-45°C Ligninolytic fungi they are use to degrade the Oxygen >0.2 mg/l DO, 10% Aerobic, minimum extremely unlike range of importunate or toxic environmental air-filled pore for air-filled pore pollutants. Common substrates used include straw, saw dust, aerobic degradation space of 10% or corn cobs. Example white rot fungus Redox potential Eh > 50 mill volts Methylotrophs bacteria that utilized methane for Heavy metals 700ppm Total content carbon and energy as their growth source it is an aerobic 2000ppm bacteria. The initial enzyme in the pathway for aerobic Contaminants Hydrocarbon 5-10% Not too toxic degradation, methane mono-oxygenase, has a broad substrate of dry weight of soil range and is active against a wide range of compounds, including the chlorinated aliphatics trichloroethylene and 1,2- dichloroethane Groundwater Pollution When the pollutants released on the ground it percolates through different interfaces of ground layer which comes in ENVIRONMENTAL FACTORS contact or mixed with the groundwater table. These substance 1. Nutrient: changes their physical and chemical properties like temperature, pH, color, total dissolve solids (TDS), dissolve The most important element which require in large quantity is oxygen (DO) etc. more than the tolerance limits causes due to Carbon that helps in the growth of microbes. Other than this anthropogenic activity of human or due to release of oxygen, hydrogen and nitrogen and is constituent about 95% chemicals by industries and use different chemicals like of the weight are required. Type of soil and its contamination pesticides in agriculture practice. Water pollution can also helps to decide the type of bioremediation required the occur due to presence of unwanted substance or the impurity concentration of sulfur and phosphorous helps to remediate in the groundwater. the 70% of the contaminants. The nutritional requirement of carbon to nitrogen ratio is 10:1, and carbon to phosphorous is It affects the plume within an aquifer. Dispersion and 30:1 movement of water within the aquifer pollute the large area of the ground.
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