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Dokument Hr. Eng INTERDISCIPLINARNO PODRUČJE STUDENTSKI ZBOR SVEUČILIŠTA U ZAGREBU 1. Vedran Bukvić, Ekonomski fakultet, Martin Ivanković, Kineziološki fakultet, Petar Labrović, Prirodoslovno-matematički fakultet, Mario Mašić, Medicinski fakultet, Anamarija Mitar, Fakultet kemijskog inženjerstva i tehnologije, Gabrijela Mitar, Prehrambeno-biotehnološki fakultet, Josip Papak, Hrvatski studiji, Ana Sikavica, Akademija dramske umjetnosti, Mate Vukšić, Fakultet političkih znanosti, Pietro Boban, Akademija likovnih umjetnosti 147 sekundi za Keniju Tragičnim napadom na akademsku zajednicu 2. travnja 2015. godine u Keniji je ubijeno 147 studenata na sveučilišnom kampusu u Garissi. Europu je dva tjedna ranije, 24. ožujka potresla smrt 150 putnika leta 9525, namjerno srušenog u francuskim Alpama. Tom prilikom stradali su pretežno Europljani, državljani Njemačke i Španjolske. Ova dva događaja drastično su se razlikovala po odjeku u javnosti. Točno dva tjedna nakon tog prešućenog događaja, inicijativom “147 sekundi za Keniju” hrvatska studentska zajednica solidarizirala se sa obiteljima žrtava i skrenula pozornost na tragediju i studentske žrtve terorizma. Cilj inicijative bio je senzibilizirati hrvatsku i međunarodnu javnost i potaknuti na širu raspravu civilnog sektora, javnih institucija i akademske zajednice o događaju u Keniji i kontekstu sukoba u kojemu se tragedija dogodila. Tog četvrtka, 16. travnja u 14 sati i sedam minuta, 147 studenata leglo na površinu ispred Hrvatskog narodnog kazališta i ostati nepomično 147 sekundi. Prema procjeni organizatora dodatnih dvjestotinjak studenata i građana Grada Zagreba. Šime Strikoman, renomirani hrvatski fotograf, inicijativu studenata ovjekovječi je milenijskom fotografijom koja je zajedno sa studentskim manifestom obišla svijet. 2. Studenti Studentskog doma Stjepan Radić (akademska godina 2013./14.) i Studentski zbor Sveučilišta u Zagrebu Studentska inicijativa "Studentski val pomoći" „Studentski val pomoći“ naziv je studentske inicijative za pomoć poplavljenim područjima na području Republike Hrvatske i Bosne i Hercegovine. Akcija se održavala u studentskom domu Stjepan Radić u Zagrebu i formalno je trajala osam dana, od 18. do 25. svibnja 2014. godine. U akciji su sudjelovali studenti studentskog doma Stjepan Radić te Studentski zbor Sveučilišta u Zagrebu koji je koordinirao gotovo svima fakultetima Sveučilišta u Zagrebu koji su se priključili akciji. Više od 400 studenata volontera svaki dan je provodilo i po 18 sati u skladištu dvorane Pauk raspoređujući i pakirajući pomoć koja je svakodnevno pristizala. Više od 100 tona robe odvedeno je sa „Save“ u Županju, Cernu, Otok, Vinkovce, Tuzlu, Maglaj, Doboj, Orašje, Domaljevac, Banjaluku, Odžak i druga mjesta koja su bila pogođena poplavama. Nakon humanitarne akcije novcima koji su prikupljeni tijekom akcije kupljeno je 50 bicikala za djecu u Gunji te 50 frižidera za stanovnike Domaljevca. “Student's wave of help“ is the name of a student initiative with the goal of helping flooded areas in Croatia and Bosnia and Herzegovina. The campaign was held in the student residence hall Stjepan Radić in Zagreb and formally lasted eight days, from the 18th to the 25th of May 2014. The campaign was executed by students from residence hall Stjepan Radic as well as the Student Council of the University of Zagreb who coordinated all of the individual faculties of the University of Zagreb which had chosen to join the initiative. More than 400 student volunteers spent up to 18 hours each day in the stockroom of "Pauk" hall sorting and packing aid items that arrived each day. More than 100 tons of goods were sent from residence hall Stjepan Radić to Županja, Cerna, Otok, Vinkovci, Tuzla, Maglaj, Doboj, Orašje, Domaljevac, Banjaluka, Odžak and other locations that were hit by flooding. After the humanitarian initiative, money that was collected during the campaign was used to buy 50 bicycles for children from Gunja as well as 50 refrigerators for the citizens of Domaljevac. PODRUČJE BIOMEDICINE I ZDRAVSTVA FARMACEUTSKO-BIOKEMIJSKI FAKULTET 3. Filip Blažević, Tamara Milekić Utjecaj lecitinsko-kitozanskih nanočestica s melatoninom na cijeljenje rane in vitro Cilj ovog istraživanja je priprava nanočestica kitozana i melatonina za topikalnu primjenu te evaluacija njihovog učinka na cijeljenje (epitelizaciju) rane in vitro u ovisnosti o sadržaju uklopljenog melatonina i svojstvima kitozana. Najnovija istraživanja u okviru razvoja funkcionalnih obloga temelje se na primjeni (bio)polimera s učinkom na cijeljenje rana, primjerice kroz utjecaj na migraciju i proliferaciju stanica kože, antimikrobni učinak te hemostatsko djelovanje. Razvijaju se polimerni terapijski nanosustavi koji mogu pridonijeti procesu cijeljenja ovisno o sadržaju djelatne tvari, polimernom sastavu i specifičnim fizičko-kemijskim svojstvima. U ovom radu lecitinsko-kitozanske nanočestice s melatoninom uspješno su pripravljene ionskom interakcijom između lecitina i kitozana. U pripravi nanočestica korištena su četiri tipa kitozana koja se međusobno razlikuju po molekulskoj masi (50-150 kDa ili 150-400 kDa) i/ili stupnju deacetilacije (75-90 % ili ˃90 %). Pripravljene su nanočestice srednjeg promjera od 245,2±2,8 do 277,1±7,6 nm, pozitivnog zeta-potencijala (23,4±0,9 do 32,5±1,2 mV) te zadovoljavajućeg sadržaja melatonina (3,8±0,3 do 4,3±0,4 %). Veličina i površinski naboj nanočestica ovisili su o molekulskoj masi i stupnju deacetilacije kitozana. Za sve ispitivane lecitinsko-kitozanske nanočestice dobiveni su slični bifazični profili produljenog oslobađanja melatonina. Izlaganje stanica suspenzijama nanočestica s melatoninom pri koncentraciji kitozana manjoj od 20 µgml-1 nije značajnije utjecalo na vijabilnost stanica, neovisno o tipu korištenog kitozana. Pri ispitivanju cijeljenja (epitelizacije) rane in vitro, modelna rana predstavljena je oštećenjem konfluentnog sloja HaCaT stanica, a cijeljenje rane proliferacijom i migracijom stanica. Za razliku od otopina kitozana koje su usporavale cijeljenje (epitelizaciju) rane in vitro, suspenzije lecitinsko-kitozanskih nanočestica odgovarajuće koncentracije kitozana ubrzavale su navedeni proces. Učinak nanočestica na cijeljenje rane in vitro ovisio je o sadržaju melatonina u nanočesticama i tipu kitozana korištenom u njihovoj pripravi. Najbrže cijeljenje rane in vitro zabilježeno je kod modelne rane izložene nanočesticama s melatoninom pripravljenima s kitozanom manje molekulske mase (50-150 kDa) i većeg stupnja deacetilacije (˃90 %). Primjenom in vitro modela rane izvršen je probir sustava s najvećim potencijalnim učinkom na epitelizaciju rane. Daljnja procjena terapijskog potencijala nanosustava uključivat će ispitivanje učinka na migraciju i proliferaciju fibroblasta te ispitivanje antimikrobnog djelovanja. The aim of this study was to prepare chitosan based nanoparticles suitable for topical melatonin delivery and to evaluate their influence on wound healing (epithelisation) in vitro in relation to melatonin content and chitosan properties. The latest trends in functional wound dressings development are focused on (bio)polymers with the potential to improve wound healing, influencing migration and proliferation of skin cells, having antimicrobial or haemostatic activity. Innovative delivery nanosystems can improve wound healing process depending on the content of the active compound, polymeric composition and physico-chemical properties. Melatonin-loaded lecithin-chitosan nanoparticles were successfully prepared by ionic interaction between lecithin and chitosan. Four types of chitosan used differed in molecular weight (50-150 kDa or 150-400 kDa) and/or deacetylation degree (75-90 % or ˃90 %). Nanoparticles had mean diameter ranging between 245,2±2,8 - 277,1±7,6 nm, positive zeta-potential (23,4±0,9 do 32,5±1,2 mV) and sufficient melatonin entrapment (3,8±0,3 do 4,3±0,4 %). Size and surface charge depended on molecular weight and deacetylation degree of chitosan. For all lecithin/chitosan nanoparticles prepared similar biphasic prolonged release profiles were obtained. Cells treatment with melatonin-loaded nanoparticles at chitosan concentration lower than 20 µgml-1 induced no decrease in cell viability, regardless of the type of chitosan used. In wound healing (epithelisation) studies, in vitro scratch test was employed. Wound healing was assayed through cell proliferation and migration. Opposite to chitosan solutions which impaired wound healing process in vitro, lecithin/chitosan nanoparticles suspension of corresponding chitosan concentration improved the mentioned process. Influence of nanoparticles on wound healing in vitro depended on melatonin content and type of chitosan used in the nanoparticles preparation. The fastest wound healing was observed in case of model wound treated with melatonin-loaded nanoparticles prepared with chitosan of lower molecular weight (50-150 kDa) and higher deacetylation degree (˃90 %). In vitro wound model allowed the selection of the nanoparticles with the highest therapeutic potential in relation with wound epithelisation. Further evaluation of nanosystems therapeutic potential will include investigation of their effect on fibroblast migration/proliferation and antimicrobial activity. 4. Ivana Fileš i Marko Tomljanović Serumske aktivnosti enzima 5'-nukleotidaze, adenozin-deaminaze i dipeptidil-peptidaze IV u osoba oboljelih od fibromialgije Nedavno utvrđene razlike u koncentracijama purinskih metabolita u plazmi i serumu osoba oboljelih od fibromialgije i zdravih osoba potakle su istraživanja koja bi objasnila utvrđene razlike
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