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Genigraphics Research Poster Template 36X48 Development of In-Situ Extraction for Point-of-Use Trace Metal Sensor in New Jersey Sediment Philip Sontag1, Katherine Dawson2, Mikayla Byron2, Matthew Esteves2, Azam Gholizadeh3, Malcolm X Shabazz Biogeochemistry Team4, Andrew D. Steen5, Ali Maher1, Mehdi Javanmard6, and Robert Miskewitz1 1Center for Advanced Infrastructure and Technology, Rutgers University 2Department of Environmental Sciences, Rutgers University 3Virginia Tech University 4Malcolm X Shabazz High School, NJ 5University of Tennessee, Earth and Planetary Sciences 6Department of Electrical Engineering, Rutgers University Abstract Square wave anodic stripping voltammogram (SWASV) of Discussion Collaborative research efforts between the Center for Advanced lead (Pb) ion in enzyme digested sediment sample • Iron (Fe)-bound and residual (sulfide)-bound lead (Pb) controls the majority of the Pb fractions in New Jersey sediment Infrastructure and Technology, Electrical Engineering, and Environmental 25 6 CC7B CC5A • Addition of β-glucosidase exoenzyme increased Pb signal in most samples Science Departments at Rutgers University have yielded a graphene oxide Pb measurment without enzyme digestion Pb measurment without enzyme digestion 5 sensor capable of measuring lead (Pb) in sediment at part per billion levels. 20 of digested sediment Pb measurment with enzyme digestion Pb measurment with enzyme digestion • Bacterial communities and metabolisms may impact enzyme-sediment 4 Parallel measurements of total Pb and Pb speciation in various sediment ICPICP-OES-OES Sediment Sediment [Pb] (mg/kg) [Pb] 15 Nitric Acid(mg/kg) alone (control): 4.4 ) digestion at varying salinities in estuarine watersheds fractions were used to produce modeled total Pb concentration from the ICPICP--OESOES Sediment Sediment [Pb] (mg/kg) [Pb] +Enzyme Extraction: 4.3 Nitric Acid(mg/kg) alone (control): 1.2 Nitric Acid alone (control): +Enzyme Extraction: 2.2 Nitric Acid alone (control): 3 4.4 • Good agreement between sensor signal and modeled total Pb sensor’s electrochemical response. Addition of exoenzyme (β-glucosidase) 1.2 +Enzyme Extraction: 4.3 Current (µA) increased sensor’s signal (factor of 2 to 10) and is likely attributed to natural (µA Current 10 +Enzyme Extraction: 2.2 concentrations was achieved in Fe-replete New Jersey sediment 2 chemical and microbial reduction fueled by the breakdown of complex 5 organic matter. Future project aims include the evaluation of microbial 1 communities in New Jersey and New York sediment and response of 0 0 microbial metabolisms to exoenzyme additions in enzyme-sediment -1 -0.9 -0.8 -0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 0 -1 -0.9 -0.8 -0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 0 Voltage vs Ag/AgCl (V) Voltage vs Ag/AgCl (V) digestion. Relationship between Sensor [Pb] and Total [Pb] Project Goals (Measured and Modeled) • Point-of-use lead (Pb) detection in less than five minutes at part per 200 Directions for Future Research y = 27.789ln(x) + 143.58 billion (ppb) levels 180 R² = 0.8248 • Analyze lead (Pb) concentrations using sensor and traditional laboratory • Model total Pb concentrations from sensor signal within 15% of measured 160 techniques in sediment from NY/NJ harbor total concentrations in New Jersey and New York sediment 140 y = 26.176ln(x) + 144.23 • Determine the impact of the microbial community on enzyme-sediment • Optimize enzyme-sediment digestion with different exoenzyme additions 120 R² = 0.9768 digestion through 16S (DNA) and response of bacteria through expression • Characterize microbial community and activity in response to exoenzyme 100 of RNA transcripts additions 80 Total [Pb] (ppm) [Pb] Total • Determine the need for regional or broad-scale chemical speciation 60 models to determine modeled total Pb concentrations in sediment 40 • Move from point-of-use sensor application to autonomous deployment 20 for real-time sediment measurements in estuarine ecosystems 0 0.00 0.50 1.00 1.50 2.00 Sensor [Pb] (ppm) Extracellular Enzymes, Breakdown of Sediment Hydrolysis Reactions Organic Matter (OM) Stacked bar chart of archaeal and bacterial diversity in 3D Printed Sample Raritan River-Bay sediment samples Sediment Stimulate Liberate Preparation Cartridge with Organic Microbial Trace Laboratory Setup Electrodes Matter Metabolism Metals Results Acknowledgements % of Lead (Pb) Bound – Four Sediment Fractions – We would like to thank: Dr. Reinfelder at Rutgers University, Dr. Steen at Cheesequake Creek University of Tennessee, and the Malcolm X Shabazz High School and Sample F1- F2- Fe Oxides F3- OM Residual- Pat Murray. Name Carbonates % % of Total % of Total Sulfides % of of Total Total SC1A 3.1 34.6 15.9 46.4 CC2B 2.9 27.6 4.4 65.1 CC7B 4.4 40.1 ND 55.5 CC5A 9.6 40.7 4.7 45.0 Contact Information SC2A 6.1 39.3 4.2 50.4 Philip Sontag – Postdoctoral Associate – Center for Advanced Infrastructure and Technology - Email: [email protected].
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