Food Enzymes
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METACYC ID Description A0AR23 GO:0004842 (Ubiquitin-Protein Ligase
Electronic Supplementary Material (ESI) for Integrative Biology This journal is © The Royal Society of Chemistry 2012 Heat Stress Responsive Zostera marina Genes, Southern Population (α=0. -
Increasing the Amylose Content of Durum Wheat Through Silencing of the Sbeiia Genes
Sestili et al. BMC Plant Biology 2010, 10:144 http://www.biomedcentral.com/1471-2229/10/144 RESEARCH ARTICLE Open Access IncreasingResearch article the amylose content of durum wheat through silencing of the SBEIIa genes Francesco Sestili1, Michela Janni1, Angela Doherty2, Ermelinda Botticella1, Renato D'Ovidio1, Stefania Masci1, Huw D Jones2 and Domenico Lafiandra*1 Abstract Background: High amylose starch has attracted particular interest because of its correlation with the amount of Resistant Starch (RS) in food. RS plays a role similar to fibre with beneficial effects for human health, providing protection from several diseases such as colon cancer, diabetes, obesity, osteoporosis and cardiovascular diseases. Amylose content can be modified by a targeted manipulation of the starch biosynthetic pathway. In particular, the inactivation of the enzymes involved in amylopectin synthesis can lead to the increase of amylose content. In this work, genes encoding starch branching enzymes of class II (SBEIIa) were silenced using the RNA interference (RNAi) technique in two cultivars of durum wheat, using two different methods of transformation (biolistic and Agrobacterium). Expression of RNAi transcripts was targeted to the seed endosperm using a tissue-specific promoter. Results: Amylose content was markedly increased in the durum wheat transgenic lines exhibiting SBEIIa gene silencing. Moreover the starch granules in these lines were deformed, possessing an irregular and deflated shape and being smaller than those present in the untransformed controls. Two novel granule bound proteins, identified by SDS- PAGE in SBEIIa RNAi lines, were investigated by mass spectrometry and shown to have strong homologies to the waxy proteins. RVA analysis showed new pasting properties associated with high amylose lines in comparison with untransformed controls. -
Product Guide
www.megazyme.com es at dr hy o rb a C • s e t a r t s b u S e m y z n E • s e m y z n E • s t i K y a s s A Plant Cell Wall & Biofuels Product Guide 1 Megazyme Test Kits and Reagents Purity. Quality. Innovation. Barry V. McCleary, PhD, DScAgr Innovative test methods with exceptional technical support and customer service. The Megazyme Promise. Megazyme was founded in 1988 with the We demonstrate this through the services specific aim of developing and supplying we offer, above and beyond the products we innovative test kits and reagents for supply. We offer worldwide express delivery the cereals, food, feed and fermentation on all our shipments. In general, technical industries. There is a clear need for good, queries are answered within 48 hours. To validated methods for the measurement of make information immediately available to the polysaccharides and enzymes that affect our customers, we established a website in the quality of plant products from the farm 1994, and this is continually updated. Today, it gate to the final food. acts as the source of a wealth of information on Megazyme products, but also is the hub The commitment of Megazyme to “Setting of our commercial activities. It offers the New Standards in Test Technology” has been possibility to purchase and pay on-line, to continually recognised over the years, with view order history, to track shipments, and Megazyme and myself receiving a number many other features to support customer of business and scientific awards. -
Cellulases: Characteristics, Sources, Production, and Applications
8 CELLULASES: CHARACTERISTICS, SOURCES, PRODUCTION, AND APPLICATIONS Xiao-Zhou Zhang and Yi-Heng Percival Zhang 8.1 INTRODUCTION lulases: (1) endoglucanases (EC 3.2.1.4), (2) exogluca- nases, including cellobiohydrolases (CBHs) (EC Cellulose is the most abundant renewable biological 3.2.1.91), and (3) β -glucosidase (BG) (EC 3.2.1.21). To resource and a low-cost energy source based on energy hydrolyze and metabolize insoluble cellulose, the micro- content ($3–4/GJ) ( Lynd et al., 2008 ; Zhang, 2009 ). The organisms must secrete the cellulases (possibly except production of bio-based products and bioenergy from BG) that are either free or cell-surface-bound. Cellu- less costly renewable lignocellulosic materials would lases are increasingly being used for a large variety of bring benefi ts to the local economy, environment, and industrial purposes—in the textile industry, pulp and national energy security ( Zhang, 2008 ). paper industry, and food industry, as well as an additive High costs of cellulases are one of the largest obsta- in detergents and improving digestibility of animal cles for commercialization of biomass biorefi neries feeds. Now cellulases account for a signifi cant share of because a large amount of cellulase is consumed for the world ’ s industrial enzyme market. The growing con- biomass saccharifi cation, for example, ∼ 100 g enzymes cerns about depletion of crude oil and the emissions of per gallon of cellulosic ethanol produced ( Zhang et al., greenhouse gases have motivated the production of bio- 2006b ; Zhu et al., 2009 ). In order to decrease cellulase ethanol from lignocellulose, especially through enzy- use, increase volumetric productivity, and reduce capital matic hydrolysis of lignocelluloses materials—sugar investment, consolidated bioprocessing ( CBP ) has been platform ( Bayer et al., 2007 ; Himmel et al., 1999 ; Zaldi- proposed by integrating cellulase production, cellulose var et al., 2001 ). -
Evaluation of Anthelmintic Activity of Carica Papaya Latex Using Pheritima Posthuma
Research Article Vol 2/Issue 1/Jan-Mar 2012 EVALUATION OF ANTHELMINTIC ACTIVITY OF CARICA PAPAYA LATEX USING PHERITIMA POSTHUMA LAKSHMI KANTA KANTHAL1*, PRASENJIT MONDAL2, SOMNATH DE4, SOMA JANA3, S. ANEELA4 AND K. SATYAVATHI1 1Koringa college of Pharmacy, Korangi, Tallarevu (M), East Godavari Dist., A.P. 2Vaageswari College Of Pharmacy, Karimnagar, A.P. 3Vaageswari Institute Of Pharmaceutical sciences, Karimnagar, A.P 4Dr.Samuel George Institute Of Pharmaceutical Sciences, Markapur, A.P. ABSTRACT The aim of present study is to evaluate Anthelmintic potential of latex of Carica papaya using Pheretima posthuma as test worms. Various concentrations (100%, 50%, and 20%) of Carica papaya latex were tested in the assay, which involved determination of time of paralysis (P) and time of death (D) of the worms. It show shortest time of paralysis (P=24.5 min) and death (D=56min) in 100% concentration, while the time of paralysis and death will increase in 50% concentration (P=28min&D=64min) and in 20% concentration (P=34min&D=74min) respectively as compare to Piperazine citrate (10mg/ml) used as standard reference (P= 24 min& D= 54) and distilled water as control. The results of present study indicated that the latex of Carica papaya showed significantly demonstrated paralysis, and also caused death of worms especially at higher concentration as compared to standard reference Piperazine citrate and control.From the result it is conclude that the latex of Carica papaya showed significant Anthelmintic activity. Key words : Pheretima posthuma, Anthelmintic, Carica papaya latex, Piperazine citrate. 1. INTRODUCTION Helminthiasis is a disease in which a part of the .2005).The papaya is a short-lived, fast-growing, body is infested with worms such as pinworm, woody, large herb to 10 or 12 feet in height. -
An Unknown Oxidative Metabolism Substantially Contributes to Soil CO2
EGU Journal Logos (RGB) Open Access Open Access Open Access Advances in Annales Nonlinear Processes Geosciences Geophysicae in Geophysics Open Access Open Access Natural Hazards Natural Hazards and Earth System and Earth System Sciences Sciences Discussions Open Access Open Access Atmospheric Atmospheric Chemistry Chemistry and Physics and Physics Discussions Open Access Open Access Atmospheric Atmospheric Measurement Measurement Techniques Techniques Discussions Open Access Biogeosciences, 10, 1155–1167, 2013 Open Access www.biogeosciences.net/10/1155/2013/ Biogeosciences doi:10.5194/bg-10-1155-2013 Biogeosciences Discussions © Author(s) 2013. CC Attribution 3.0 License. Open Access Open Access Climate Climate of the Past of the Past Discussions An unknown oxidative metabolism substantially contributes to soil Open Access Open Access Earth System CO2 emissions Earth System Dynamics 1,* 1,2 3 1 4 5 Dynamics1 3 V. Maire , G. Alvarez , J. Colombet , A. Comby , R. Despinasse , E. Dubreucq , M. Joly , A.-C. Lehours , Discussions V. Perrier5, T. Shahzad1,**, and S. Fontaine1 1INRA, UR 874 UREP, 63100 Clermont-Ferrand, France Open Access Open Access 2Clermont Universite,´ VetAgro Sup, 63000, Clermont-Ferrand, France Geoscientific Geoscientific 3University of Clermont Ferrand, UMR 6023 LMGE, 63177 Aubiere,` France Instrumentation Instrumentation 4INRA, UMR 1095 UBP, 63100 Clermont-Ferrand, France Methods and Methods and 5Montpellier SupAgro, UMR 1208 IATE, 34060 Montpellier, France *present address: Department of Biological Sciences, Macquarie -
Chymopapain Chemonucleolysis: CT Changes After Treatment
321 Chymopapain Chemonucleolysis: CT Changes after Treatment Lindell R. Gentry 1. 2 Chymopapain chemonucleolysis is now used extensively in this country to treat lumbar Patrick A. Turski1 disk herniation. Despite increasing experience in patient selection, there continue to be Charles M. Strother1 patients who do not respond to treatment and require diagnostic reevaluation. Interpre Manucher J. Javid3 tation of postchemonucleolysis computed tomographic (CT) scans in these patients Joseph F. Sackett 1 requires a knowledge of the CT changes that normally occur after treatment with chemonucleolysis. To define these temporal changes, a prospective CT evaluation was performed of 29 treated interspaces in 26 patients who returned for routine postche monucleolysis follOW-Up. Despite a successful clinical response in 17 of 21 patients, changes in the size, location, shape, homogeneity, and density of the disk herniation were uncommon at the 6 week follow-up. In 24 treated interspaces, the most common changes at 6 week CT follow-up were the development of vacuum phenomenon in three (12.5%) and a slight decrease in the size of two (8.3%) disk herniations. A successful response was noted in 17 of 21 patients scanned at 6 month follow-up, with five (22.7%) of 22 injected interspaces exhibiting vacuum phenomenon and 13 (59.1%) interspaces showing an observable decrease in the size of the disk herniation. Early improvement of sciatica after chemonucleolysis often occurs without a change in the size of the disk herniation and may be mediated by chymopapain-induced disk-space narrowing. Con tinued improvement may be accompanied by both a decrease in the disk height and a reduction in the size of the disk protrusion. -
Collection of Information on Enzymes a Great Deal of Additional Information on the European Union Is Available on the Internet
European Commission Collection of information on enzymes A great deal of additional information on the European Union is available on the Internet. It can be accessed through the Europa server (http://europa.eu.int). Luxembourg: Office for Official Publications of the European Communities, 2002 ISBN 92-894-4218-2 © European Communities, 2002 Reproduction is authorised provided the source is acknowledged. Final Report „Collection of Information on Enzymes“ Contract No B4-3040/2000/278245/MAR/E2 in co-operation between the Federal Environment Agency Austria Spittelauer Lände 5, A-1090 Vienna, http://www.ubavie.gv.at and the Inter-University Research Center for Technology, Work and Culture (IFF/IFZ) Schlögelgasse 2, A-8010 Graz, http://www.ifz.tu-graz.ac.at PROJECT TEAM (VIENNA / GRAZ) Werner Aberer c Maria Hahn a Manfred Klade b Uli Seebacher b Armin Spök (Co-ordinator Graz) b Karoline Wallner a Helmut Witzani (Co-ordinator Vienna) a a Austrian Federal Environmental Agency (UBA), Vienna b Inter-University Research Center for Technology, Work, and Culture - IFF/IFZ, Graz c University of Graz, Department of Dermatology, Division of Environmental Dermatology, Graz Executive Summary 5 EXECUTIVE SUMMARY Technical Aspects of Enzymes (Chapter 3) Application of enzymes (Section 3.2) Enzymes are applied in various areas of application, the most important ones are technical use, manufacturing of food and feedstuff, cosmetics, medicinal products and as tools for re- search and development. Enzymatic processes - usually carried out under mild conditions - are often replacing steps in traditional chemical processes which were carried out under harsh industrial environments (temperature, pressures, pH, chemicals). Technical enzymes are applied in detergents, for pulp and paper applications, in textile manufacturing, leather industry, for fuel production and for the production of pharmaceuticals and chiral substances in the chemical industry. -
Biocatalysis, Enzyme Engineering and Biotechnology G
P1: SFK/UKS P2: SFK BLBS102-c07 BLBS102-Simpson March 21, 2012 11:12 Trim: 276mm X 219mm Printer Name: Yet to Come 7 Biocatalysis, Enzyme Engineering and Biotechnology G. A. Kotzia, D. Platis, I. A. Axarli, E. G. Chronopoulou, C. Karamitros, and N. E. Labrou Preface Enzyme Utilisation in Industry Enzyme Structure and Mechanism Enzymes Involved in Xenobiotic Metabolism and Nomenclature and Classification of Enzymes Biochemical Individuality Basic Elements of Enzyme Structure Phase I The Primary Structure of Enzyme Phase II The Three-Dimensional Structure of Enzymes Phase III Theory of Enzyme Catalysis and Mechanism Acknowledgements Coenzymes, Prosthetic Groups and Metal Ion Cofactors References Kinetics of Enzyme-Catalysed Reactions Thermodynamic Analysis Abstract: Enzymes are biocatalysts evolved in nature to achieve Enzyme Dynamics During Catalysis the speed and coordination of nearly all the chemical reactions that Enzyme Production define cellular metabolism necessary to develop and maintain life. Enzyme Heterologous Expression The application of biocatalysis is growing rapidly, since enzymes The Choice of Expression System offer potential for many exciting applications in industry. The ad- Bacterial Cells vent of whole genome sequencing projects enabled new approaches Mammalian Cells for biocatalyst development, based on specialised methods for en- Yeast zyme heterologous expression and engineering. The engineering of Filamentous Fungi enzymes with altered activity, specificity and stability, using site- Insect Cells directed mutagenesis and directed evolution techniques are now Dictyostelium discoideum Trypanosomatid Protozoa well established. Over the last decade, enzyme immobilisation has Transgenic Plants become important in industry. New methods and techniques for en- Transgenic Animals zyme immobilisation allow for the reuse of the catalysts and the Enzyme Purification development of efficient biotechnological processes. -
Microbial Cellulases: a Review on Strain Development, Purification, Characterization and Their Industrial Applications
Open Access Journal of Bacteriology and Mycology Review Article Microbial Cellulases: A Review on Strain Development, Purification, Characterization and their Industrial Applications Sher H1,2, Zeb N1,3, Zeb S4, Ali A4, Aleem B1, Iftikhar F1, Rahman SU1 and Rashid MH1,2* Abstract 1National Institute for Biotechnology and Genetic In this advance era, the enzymes are considered as a core kernel of white Engineering (NIBGE), Faisalabad, Pakistan biotechnology and their demand is increasing day by day. According to report 2Pakistan Institute of Engineering and Applied Sciences published in Research and Markets (ID: 5009185), the estimated global market (PIEAS), Islamabad, Pakistan for industrial enzymes were USD 10.0 billion in 2019, which is continuously 3Department of Biotechnology and Microbiology, Women increasing as it is expected to reach about USD 14.7 billion by 2022. Among University Mardan, KP, Pakistan all enzymes, cellulases are the major group of enzymes act synergistically in 4Department of Microbiology, Abdul Wali Khan breakdown of cellulose, that facilitates its conversion to various value-added University Mardan, KP, Pakistan products and also offer several other important applications at industrial scale. #First two authors contributed equally The hyper production of cellulases are required to overcome their demand of *Corresponding author: Muhammad Hamid Rashid, global market. Cellulases production can be enhanced by strain improvement as Industrial Biotechnology Division, National Institute well as using advance fermentation -
Guide to Biotechnology 2008
guide to biotechnology 2008 research & development health bioethics innovate industrial & environmental food & agriculture biodefense Biotechnology Industry Organization 1201 Maryland Avenue, SW imagine Suite 900 Washington, DC 20024 intellectual property 202.962.9200 (phone) 202.488.6301 (fax) bio.org inform bio.org The Guide to Biotechnology is compiled by the Biotechnology Industry Organization (BIO) Editors Roxanna Guilford-Blake Debbie Strickland Contributors BIO Staff table of Contents Biotechnology: A Collection of Technologies 1 Regenerative Medicine ................................................. 36 What Is Biotechnology? .................................................. 1 Vaccines ....................................................................... 37 Cells and Biological Molecules ........................................ 1 Plant-Made Pharmaceuticals ........................................ 37 Therapeutic Development Overview .............................. 38 Biotechnology Industry Facts 2 Market Capitalization, 1994–2006 .................................. 3 Agricultural Production Applications 41 U.S. Biotech Industry Statistics: 1995–2006 ................... 3 Crop Biotechnology ...................................................... 41 U.S. Public Companies by Region, 2006 ........................ 4 Forest Biotechnology .................................................... 44 Total Financing, 1998–2007 (in billions of U.S. dollars) .... 4 Animal Biotechnology ................................................... 45 Biotech -
Studies of Barley Limit Dextrinase I I
b ¡\il i: ilq.5l¡Tt-!iÈ' ta. S.S LIBRARY STUDIES OF BARLEY LIMIT DEXTRINASE I I I I by I l I ¡ I Michael John Sissons ì B.Ag.Sc. (University of Adelaide)' I I M.Ag.Sc. (La Trobe UniversitY) i l A thesis submitted to the University of Adelaide for the degree of Doctor of Philosophy Departnent of Plant Science, Waite Agricultural Resea¡ch Institute, Glen Osmond, South Australia October, 1991 LIST OF CONTENTS Contents Page No. Summary i-ü Statement of originality and consent for photocopy or loan lU Acknowledgements iv List of publications v Chapter 1: LITERATURE REVIEW 1.1 Introduction I 1.2 Properties of limit dextrinase 2 t.2.r Purification 2 1.2.2 Enzyme properties 3 t.2.3 Effect of inhibitors 3 r.2.4 Polymorphism 7 t.2.5 Substrate specificity 7 1.2.5.1 Action of limit dextrinase on oligosaccharides 7 t.2.5.2 Polysaccharides 7 1.3 Methods of Assay 9 1.3.1 Detection of Limit Dextrinase Activity in Electrophoretic Gels 10 t.4 Synthesis of limit dextrinase t2 1.4.1 Mechanism of Increase in Limit Dextrinase Activity during Germination 13 1.5 Effect of Barley Genotype and Environment on Limit Dextrinase ActivitY 15 1.6 Limit dextrinase - Role in Malting and Brewing 16 1.6.1 Effect of Kilning on Limit Dextrinase Activity 18 r.6.2 Effect of Mashing on Limit Dextrinase Activity 18 1.6.3 Limit 0extrinase-Role in Speciality Brewing, Distiling and Related Iridustries 2l 1.6.4 Relationship beween Limit Dextrinase Activity, Wort Fermentability and Alcohol Production 22 r.7 Role of Limit Dextrinase in Starch Degradation 23 1.8 Conclusions 24 Chapter