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United States Patent [19] [11] Patent Number: 5,830,741 Dwulet Et Al

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United States Patent [19] [11] Patent Number: 5,830,741 Dwulet Et Al US005830741A United States Patent [19] [11] Patent Number: 5,830,741 Dwulet et al. [45] Date of Patent: *Nov. 3, 1998 [54] COMPOSITION FOR TISSUE DISSOCIATION Suggs et al. (1992) J. Vasc. Surg., 15(1), “Enzymatic Har CONTAINING COLLAGENASE I AND II vesting of Adult Human Saphenous Vein Endothelial Cells: FROM CLOSTRIDI UM HISTOLYTI C UM AND A Use Of Chemically De?ned Combination of TWo Puri?ed NEUTRAL PROTEASE Enzymes to Attain Viable Cell Yields Equal to Those Attained by Crude Bacterial Collagenase Preparations”, pp. [75] Inventors: Francis E. DWulet, Greenwood; 205—213. Marilyn E. Smith, McCordsville, both Bond et al., “Characterization of the Individual Collagenases of Ind. from Clostridium histolyticum”, 1984, pp. 3085—3091, Bio chemistry vol. 23, No. 13. [73] Assignee: Boehringer Mannheim Corporation, Bond et al., “Puri?cation and Separation of Individual Indianapolis, Ind. Collagenases of Clostridium histolyticum Using Red Dye Ligand Chromatography”, 1984, pp. 3077—3085, Biochem. [ * ] Notice: The term of this patent shall not extend vol. 23 No. 13. beyond the expiration date of Pat. No. Dean et al., “Protein Puri?cation Using Immobilized Trizine 5,753,485. Dyes”, 1979, pp. 301—319, Journal of Chromatography, 165. [21] Appl. No.: 760,893 Emo d et al., “Five Sepharose—Bound Ligands for the Chromatographic Puri?cation of Clostridium Collagenase [22] Filed: Dec. 6, 1996 and Clostripain”, 1977, pp. 51—56, FEBS Letters vol. 77 No. [51] Int. Cl.6 C12N 9/52; C12N 9/00 1. Hatton et al., “The role of proteolytic enzymes derived from [52] US. Cl. .. 435/220; 435/183 [58] Field of Search . ...... .. 435/220, 183 crude bacterial collagenase in the liberation of hepatocytes from rat liver”, 1983, pp. 311—318, J.Biochem. 137. [56] References Cited He?ey et al., “Enzymatic isolation of cells from bone: cytotoxic enzymes of bacterial collagenase”, 1981, pp. U.S. PATENT DOCUMENTS C234—C238, Am. J. Physiology 240. 3,201,325 8/1965 Barton ................................... .. 435/220 He?ey et al., “Enzymatic Isolation of Cells from Neonatal 3,705,083 12/1972 Chiulli et al. ......................... .. 435/220 Calvaria Using TWo Puri?ed Enzymes from Clostridium 3,821,364 6/1974 Chiulli et al. ...................... .. 424/9467 histolyticum”, 1983, pp. 227—236, Experimental Cell 4,256,836 3/1981 IsoWa et al. .... .. .. 435/68.1 Research 149. 4,431,544 2/1984 Atkinson et al. 210/635 He?ey, “Utilization of FPLC—Puri?ed Bacterial Collagenase 4,431,546 2/1984 Hughes et al. 210/656 from the Isolation of Cells from Bone”, 1987, pp. 505—516, 4,732,758 3/1988 Hurion et al. .. 424/94.2 Journal of Bone and Mineral Research vol. 2 No. 7. 4,797,213 1/1989 Parisius et al. 210/651 Kobayashi et al., “Puri?cation and Characterization of Myo 4,868,121 9/1989 Scharp et al. 435/268 sin from Bovine Thyroid”, Nov. 25, 1977, pp. 8285—8291, 4,873,359 10/1989 Chmurny et al. 560/40 4,946,792 8/1990 O’Leary ...... .. 435/268 The Journal of Biological Chemistry, vol. 252, No. 22. 5,079,160 1/1992 Lacy et al. 435/381 Kono, “Puri?cation and Partial Characterization of Col 5,116,615 5/1992 Gokcen et al. .. 424/94.2 lagenolytic Enzymes from Clostridium histolyticum”, Mar. 5,120,656 6/1992 O’Leary et al. .. 435/1.1 1968, pp. 1106—1114, Biochemistry vol. 7 No. 3. 5,173,295 12/1992 Wehling . .. 424/9467 Kula et al., “Consecutive Use of 00—Aminoa1kylagaroses. 5,177,017 1/1993 Lin et al. .. 435/252.33 Res. and Purif. of Clostripain and Collagenase from C. 5,273,904 12/1993 Langley ...... .. 435/283.1 Histolyticum ”, 1 976, pp. 38 9—396, 5,322,790 6/1994 Scharp et al. 435/268 Bioch.&Bioph.Res. Comm. vol. 69 No.2. 5,332,503 7/1994 Lee etal. .... .. 210/635 McShane et al., “Protease Activity in Pancreatic Islet Iso 5,422,261 6/1995 Lee et al. .............................. .. 435/219 lation by Enzymatic Digestion”, 1989, pp. 126—128, Dia FOREIGN PATENT DOCUMENTS betes vol. 38 Suppl. 1. 0 191 613 8/1986 European Pat. Off. (List continued on next page.) 91/14447 10/1991 WIPO . 96/00283 1/1996 WIPO . Primary Examiner—Jon P. Weber Attorney, Agent, or Firm—Brent A. Harris; D. Michael OTHER PUBLICATIONS Young; Marilyn L. Amick Tavakol et al. (1984) Surgical Forum, 37, “Enhanced Dis [57] ABSTRACT solution of Nucleus Pulposus: Combined Enzyme Approach”, pp. 491—494. An enzyme composition for dissociating tissues is disclosed. Maruyama et al. (1987) J. Pharmacol. Meth., 18, “Prepara The composition comprises puri?ed collagenase I and col tion of Single Smooth Muscle Cells from Guinea Pig Taenia lagenase II from Clostridium histolyticum in a mass ratio of C011 by Combination of Puri?ed Collagenase and Papain”, about 0.3 to 0.6 and a neutral protease such that the ratio of pp. 151—161. total FITC casein units of activity to Wunsch units of activity Maruyama et al. (1988) J. Pharmacol. Meth., 19, “Improve in the composition is about 250:1 to about 600:1. The ment of a Procedure for Preparing Single Smooth Muscle preferred neutral protease is thermolysin. Cells from Guinea Pig Taenia C011 by Puri?ed Collagenase and Papain”, pp. 155—164. 6 Claims, No Drawings 5,830,741 Page 2 OTHER PUBLICATIONS Wolters et al., “An analysis of the role of collagenase and Patel, “Biotechnology: Applications and Research”, 1985, protease in the enzymatic dissociaiton of rat pancreas for pp. 534—5 62, published by Technornic Publishing Company, islet isolation”, 1992, pp. 735—742, Diabetologia 35. Inc., Lancaster Pennsylvania. Sharefkin et al., “Adult human endothelial cell enZyrnatic Wolters et al., “Different Roles of Class I and Class II harvesting”, 1986, pp. 567—577, Journal of Vascular Sur Clostridium Histolyticum Collagenase in Rat Pancreatic gery vol. 4 No. 6. Islet Isolation”, 1995, pp. 227—233, Diabetes vol. 44. Silink et al., “y—Glutarnyl Hydrolase (Conjugase)”, Aug. 10, 1975, pp. 5982—5994, The Journal ofBiological Chemistry, Worthington, “Worthington EnZyrne Manual—enZyrnes and vol. 250, No. 15. related biochernicals”, 1988, pp. 93—101, Worthington Bio Stevens et al., “Dissociation of Lung Tissue into Monodis persed Cells by Use of Puri?ed Collagenase”, 1979, pp. chernical Corporation. 460—470, J. Appl. Biochem. 1. H.O. Jauregui, et al., “Primary Cultures of Rat Hepatocytes Van Wart et al., “Cornplernentary Substrate Speci?cities of Class I and Class II Collagenases from C lostria'ium histolyti in HolloW Fiber Charnbers”, In Wtro Cell Dev. Biol, 30A, cum ”, 1985, pp. 6520—6526, Biochemistry vol. 24 No. 23. 23—29 (1994). 5,830,741 1 2 COMPOSITION FOR TISSUE DISSOCIATION the presence of de?ned amounts of both C. histolyticum CONTAINING COLLAGENASE I AND II collagenase class I (collagenase I) and collagenase class II FROM CLOSTRIDI UM HISTOLYTIC UM AND A (collagenase II) enZymes With at least tWo neutral proteases NEUTRAL PROTEASE has been found to in?uence the efficacy of pancreatic islet isolation. HoWever, there still exists a need for identifying FIELD OF THE INVENTION optimiZed enZyme compositions Which provide for rapid This invention relates to compositions for the enzymatic dissociation of tissue and recovery of a greater number of dissociation of extracellular tissue matrices to alloW tissue viable cells. remodeling and the ef?cient isolation of viable cells and cell SUMMARY OF THE INVENTION clusters from tissue. 10 The present invention provides an enZyme composition BACKGROUND OF THE INVENTION prepared by combining de?ned masses of puri?ed proteases, The enZymatic dissociation of tissue into individual cells including collagenase I and collagenase II from C. histolyti and cell clusters is useful in a Wide variety of laboratory, cum and an amount of an endoprotease such that the ratio of diagnostic and therapeutic applications. These applications 15 the total FITC casein units of activity of the enZyme com involve the isolation of many types of cells for various uses, position to the Wunsch units of activity of the masses of including microvascular endothelial cells for small diameter collagenase I and collagenase II is about 85:1 to about synthetic vascular graft seeding, hepatocytes for gene 3,900:1. When used in tissue dissociation protocols for cell therapy, drug toxicology screening and extracorporeal liver isolation the resulting enZyme composition functions to assist devices, chondrocytes for cartilage regeneration, and 20 effect improved, more rapid dissociation of extracellular islets of Langerhans for the treatment of insulin-dependent matrices and it alloWs recovery of higher yields of the diabetes mellitus. EnZyme treatment Works to fragment desired cells With improved viability relative to the number extracellular matrix proteins and proteins Which maintain and viability of cells harvested using enZyme compositions cell-to-cell contact. Since collagen is the principle protein of the prior art, including crude collagenase compositions. component of tissue ultrastructure, the enZyme collagenase In another embodiment of this invention there is provided has been frequently used to accomplish the desired tissue an enZyme composition consisting essentially of collagenase disintegration. I and collagenase II from C. histolyticum and an Different forms of crude bacterial collagenase derived endoprotease, Wherein the ratio of the mass of collagenase II to the mass of collagenase I plus the mass of collagenase from Clostridium histolyticum are commercially available 30 and are used to dissociate cells and cell clusters from tissue. II in the enZyme composition is about 0.3 to about 0.6. The These crude collagenases, derived from cell culture resulting enZyme composition is also an improvement over supernatants, typically contain a mixture of protease previously knoWn compositions because of its ability to enZymes (exhibiting both collagenolytic and non-speci?c more rapidly dissociate extracellular tissue matrices and alloW recovery of a larger number of viable cells.
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