Α-Smooth Muscle Actin (ACTA2) Rabbit Pab

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α-Smooth Muscle Actin (ACTA2) Rabbit pAb

Catalog No.: A1011 6 Publications

Basic Information Background

Catalog No. The protein encoded by this gene belongs to the actin family of proteins, which are A1011 highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major Observed MW constituent of the contractile apparatus, while beta and gamma actins are involved in 42kDa the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively Calculated MW spliced variants, encoding the same protein, have been identified. 42kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:1000 Gene ID Swiss Prot 59 P62736 IHC 1:50 - 1:100 Immunogen 1:100 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human α-Smooth Muscle Actin (ACTA2) (NP_001135417.1).

Synonyms AAT6;ACTSA;MYMY5;ACTA2;actin

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody (A1011) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded human placenta using α- embedded rat ovary using α-Smooth embedded mouse lung using α-Smooth Smooth Muscle Actin (ACTA2) antibody Muscle Actin (ACTA2) antibody (A1011) at Muscle Actin (ACTA2) antibody (A1011) at (A1011) at dilution of 1:100 (40x lens). dilution of 1:100 (40x lens). dilution of 1:100 (40x lens).

Immunofluorescence analysis of rat rectum Immunofluorescence analysis of human Immunofluorescence analysis of mouse using α-Smooth Muscle Actin (ACTA2) smooth muscle using α-Smooth Muscle colon using α-Smooth Muscle Actin Rabbit pAb (A1011) at dilution of 1:100 Actin (ACTA2) Rabbit pAb (A1011) at (ACTA2) Rabbit pAb (A1011) at dilution of (40x lens). Blue: DAPI for nuclear staining. dilution of 1:100 (40x lens). Blue: DAPI for 1:100 (40x lens). Blue: DAPI for nuclear nuclear staining. staining.