Monoclonal Antibody to CD161 / KLRB1 - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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SM1611PT Monoclonal Antibody to CD161 / KLRB1 - Purified

Alternate names: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A Quantity: 25 µg Concentration: 1.0 mg/ml Background: Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. CD161 is expressed by almost all NK cells and with a small subset of CD3+ve T cells. CD161 is a homodimeric cell surface protein, comprising two chains of 40-44kD. Uniprot ID: Q12918 NCBI: NP_002249.1 GeneID: 3820 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), AM03095PU-N Controls: Clone: B199.2 Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line. Format: State: Liquid purified IgG fraction. Purification: Affinity Chromatography on Protein G. Buffer System: PBS, pH 7.4 Preservatives: 0.09% Sodium Azide Applications: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognises the CD161 cell surface antigen. Clone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140626 1 / 2 SM1611PT: Monoclonal Antibody to CD161 / KLRB1 - Purified

Species Reactivity: Tested: Human. Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Bennett IM, Zatsepina O, Zamai L, Azzoni L, Mikheeva T, Perussia B. Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. J Exp Med. 1996 Nov 1;184(5):1845-56. PubMed PMID: 8920872. 2. Azzoni L, Zatsepina O, Abebe B, Bennett IM, Kanakaraj P, Perussia B. Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells. J Immunol. 1998 Oct 1;161(7):3493-500. PubMed PMID: 9759869.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140626 2 / 2