Polyclonal Antibody to Sestrin-1 (SESN1) (N-Term

AP09869PU-N OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Polyclonal Antibody to Sestrin-1 (SESN1) (N-term) - Aff - Purified Alternate names: PA26, SEST1, Sestrin 1 Catalog No.: AP09869PU-N Quantity: 0.1 mg Concentration: 0.64-0.72 mg/ml (lot specific) Background: Sestrin 1 (SESN1), located on rat chromosome 20q13, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Sestrin 1 is implicated in the inhibition of cell growth. It has been reported that Sestrin 1 activates AMP-responsive protein kinase (AMPK) and targets it to phosphorylate TSC2, a tumor suppressor. Phosphorylation of TSC2 stimulates its GAP activity which inhibits the activity of mTOR, a positive regulator of cell growth, thus leading to inhibition of cell proliferation and growth (4). Inhibition of mRNAs from sestrin 1 and 3 genes has been shown to substantially increase intracellular ROS levels. This increase in ROS is linked to Ras-induced mutagenesis which is one of the most common events in cancer (5). Sestrin 1 was also identified as a candidate gene for heterotaxia in humans (6). Recent studies have identified that AMPK is an essential mediator of tumor suppressor LKB1 and is suppressed in cancer cells (7). Since activity of AMPK is regulated by sestrin 1, this protein could be a promising target for cancer treatment and therapy. Sestrin 1 is approximately a 66.1kDa protein in humans (551 amino acids) and 55.4kDa protein in mouse and rat (462 amino acids). Uniprot ID: Q9Y6P5 NCBI: NP_055269 GeneID: 27244 Host: Rabbit Immunogen: Synthetic peptide corresponding to unique epitope on SESN1 from N-terminal (aa 1-50) region. Remarks: The peptide was post-synthetically covalently modified to achieve desired antigenicity. Format: State: Liquid purified Ig fraction Purification: Affinity Chromatography on immobilized antigen Buffer System: Stabilization buffer Applications: Suitable for use in ELISA (< 1/10,000) and Western Blot (> 1/500).

For research and in vitro use only. Not for diagnostic or therapeutic work. OG/20130614 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 AP09869PU-N: Polyclonal Antibody to Sestrin-1 (SESN1) (N-term) - Aff - Purified Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody will label ~69kDa protein in Western blot positive control samples for SESN1 and several other tissues. Species: Human, Mouse and Rat. Other species not tested. Add. Information: Antigenic blocking peptide (AP09869CP-N) for Sestrin-1 is also available separately. Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Meiller A, Alvarez S, Drané P, Lallemand C, Blanchard B, Tovey M, et al. p53-dependent stimulation of redox-related genes in the lymphoid organs of gamma-irradiated--mice identification of Haeme-oxygenase 1 as a direct p53 target gene. Nucleic Acids Res. 2007;35(20):6924-34. Epub 2007 Oct 11. PubMed PMID: 17933770. 2. Jonsson TJ, Lowther WT. The peroxiredoxins repair proteins. Subcell Biochem. 2007; 44:115-141. 3. Budanov AV, Sablina AA, Feinstein E, Koonin EV, Chumakov PM. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD. Science. 2004 Apr 23;304(5670):596-600. PubMed PMID: 15105503. 4. Budanov AV, Karin M. p53 target genes sestrin 1 and sestrin 2 connect genotoxic stress and mTOR signaling. Cell. 8 August 2008; 134(3):451-460. 5. Kopnin PB, Agapova LS, Kopnin BP, Chumakov PM. Repression of sestrin family genes contributes to oncogenic Ras-induced reactive oxygen species up-regulation and genetic instability. Cancer Res. 2007 May 15;67(10):4671-8. PubMed PMID: 17510393. 6. Peeters H, Debeer P, Bairoch A, Wilquet V, Huysmans C, Parthoens E, et al. PA26 is a candidate gene for heterotaxia in humans: identification of a novel PA26-related gene family in human and mouse. Hum Genet. 2003 May;112(5-6):573-80. Epub 2003 Feb 27. PubMed PMID: 12607115. 7. Shaw RJ. LKB1 and AMP-activated protein kinase control of mTOR signalling and growth. Acta Physiol (Oxf). 2009 May;196(1):65-80. doi: 10.1111/j.1748-1716.2009.01972.x. Epub 2009 Feb 19. PubMed PMID: 19245654. Pictures: Western Blot of Sestrin-1 antibody (Cat#AP09869PU-N) at 1/500 dilution.