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File Download Metabolomic signature of exposure and response to citalopram/escitalopram in depressed outpatients Sudeepa Bhattacharyya, University of Arkansas Ahmed T. Ahmed, Mayo Clinic Matthias Arnold, Duke University Duan Liu, Mayo Clinic Chunqiao Luo, University of Arkansas Hongjie Zhu, Sanofi Siamak Mahmoudiandehkordi, Duke University Drew Neavin, Mayo Clinic Gregory Louie, Duke University Boadie Dunlop, Emory University Only first 10 authors above; see publication for full author list. Journal Title: Translational Psychiatry Volume: Volume 9, Number 1 Publisher: Springer Nature [academic journals on nature.com]: Fully open access journals | 2019-07-04, Pages 173-173 Type of Work: Article | Final Publisher PDF Publisher DOI: 10.1038/s41398-019-0507-5 Permanent URL: https://pid.emory.edu/ark:/25593/tvf11 Final published version: http://dx.doi.org/10.1038/s41398-019-0507-5 Copyright information: © 2019, The Author(s). This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). Accessed September 25, 2021 10:33 AM EDT Bhattacharyya et al. Translational Psychiatry (2019) 9:173 https://doi.org/10.1038/s41398-019-0507-5 Translational Psychiatry ARTICLE Open Access Metabolomic signature of exposure and response to citalopram/escitalopram in depressed outpatients Sudeepa Bhattacharyya 1,AhmedT.Ahmed2, Matthias Arnold 3,4,DuanLiu 5, Chunqiao Luo1, Hongjie Zhu6, Siamak Mahmoudiandehkordi3, Drew Neavin5, Gregory Louie 3,BoadieW.Dunlop7,MarkA.Frye2,LieweiWang5, Richard M. Weinshilboum5, Ranga R. Krishnan8,A.JohnRush3,9,10 and Rima Kaddurah-Daouk 3,11,12 Abstract Metabolomics provides valuable tools for the study of drug effects, unraveling the mechanism of action and variation in response due to treatment. In this study we used electrochemistry-based targeted metabolomics to gain insights into the mechanisms of action of escitalopram/citalopram focusing on a set of 31 metabolites from neurotransmitter- related pathways. Overall, 290 unipolar patients with major depressive disorder were profiled at baseline, after 4 and 8 weeks of drug treatment. The 17-item Hamilton Depression Rating Scale (HRSD17) scores gauged depressive symptom severity. More significant metabolic changes were found after 8 weeks than 4 weeks post baseline. Within the tryptophan pathway, we noted significant reductions in serotonin (5HT) and increases in indoles that are known to be influenced by human gut microbial cometabolism. 5HT, 5-hydroxyindoleacetate (5HIAA), and the ratio of 5HIAA/ 5HT showed significant correlations to temporal changes in HRSD17 scores. In the tyrosine pathway, changes were observed in the end products of the catecholamines, 3-methoxy-4-hydroxyphenylethyleneglycol and vinylmandelic 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; acid. Furthermore, two phenolic acids, 4-hydroxyphenylacetic acid and 4-hydroxybenzoic acid, produced through noncanconical pathways, were increased with drug exposure. In the purine pathway, significant reductions in hypoxanthine and xanthine levels were observed. Examination of metabolite interactions through differential partial correlation networks revealed changes in guanosine–homogentisic acid and methionine–tyrosine interactions associated with HRSD17. Genetic association studies using the ratios of these interacting pairs of metabolites highlighted two genetic loci harboring genes previously linked to depression, neurotransmission, or neurodegeneration. Overall, exposure to escitalopram/citalopram results in shifts in metabolism through noncanonical pathways, which suggest possible roles for the gut microbiome, oxidative stress, and inflammation-related mechanisms. Introduction worldwide1. Selective serotonin reuptake inhibitors Major depressive disorder (MDD) is a common, often (SSRIs) are common first-line treatments for MDD2,3. disabling condition affecting over 300 million individuals They are believed to increase the extracellular availability of the neurotransmitter serotonin by limiting its reab- sorption into the presynaptic cell, so that serotonin levels Correspondence: Rima Kaddurah-Daouk ([email protected]) are increased in the synaptic cleft and available for 1Department of Biomedical Informatics, University of Arkansas for Medical binding to postsynaptic receptors. Responses to anti- Sciences, Little Rock, AR, USA depressant medications are modest. Only about half the 2Department of Psychiatry and Psychology, Mayo Clinic, Rochester, MN, USA Full list of author information is available at the end of the article. patients respond to the first medication; only one in three These authors contributed equally: Ahmed T. Ahmed, Matthias Arnold © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a linktotheCreativeCommons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Bhattacharyya et al. Translational Psychiatry (2019) 9:173 Page 2 of 14 achieves symptom remission, which is the virtual absence – The metabolomic signature of exposure to 4 of symptoms and the aim of treatment . Some patients do escitalopram/citalopram: which metabolite changes well on a single medication, while others require medi- occurred from baseline to week 4, and from baseline cation combinations or alternative interventions. Clinical to week 8 of treatment? symptoms are insufficient to guide appropriate treatment – The metabolomic signature of response: which selection5 and, presently, treatments are therefore selected metabolomic changes were related to changes in 6,7 empirically relying on a “trial and error” approach . depressive symptoms (HRSD-17), longitudinally, in Metabolomics, a promising new approach to under- the overall population and also in responders versus standing depression and other neuropsychiatric dis- nonresponders? – orders8 11, could help inform treatment selection12,13. – The interrelationships between metabolites: what are Metabolomic profiles provide informative readouts on the relationships among metabolites, both within pathways and biological networks implicated in various and between pathways, before and after treatment diseases or their treatments. Metabolomic signatures with the drug? have been identified for several psychiatric disorders, such as MDD14, bipolar disorder15,16,andschizo- Methods – phrenia17 19. Most studies of mood disorders have Study design and participants implicated tryptophan (TRP), tyrosine, and purine We used samples from the Mayo Clinic NIH- metabolism, since historically, neurotransmission and Pharmacogenomics Research Network-Antidepressant serotonergic signaling were key focus areas of investi- Pharmacogenomics Medication Study (PGRN-AMPS) gation14. The TRP pathway along with its three branches which recruited a total of 803 MDD patients39. Patient of metabolism to serotonin/melatonin/5-hydro- selection, symptomatic evaluation, and blood sample xyindoleacetate, kynurenine (KYN), and indole deriva- collection for the PGRN-AMPS clinical trial have been – – tives, seems to be affected in the depressed state20 28. described elsewhere24,38 40. Briefly, MDD patients were The purine pathway, whose regulation seems to be required to have a baseline HRSD17 score ≥ 14, and all connected to TRP metabolism, has also been implicated patients who completed 8 weeks of treatment (n = 290) in depression and other psychiatric disorders29.Among were treated with one of the two SSRIs, citalopram or patients in remission from a major depressive episode, a escitalopram. Depressive symptoms were assessed with metabolomic signature that included methionine, glu- HRSD17 at baseline, week 4, and week 8 of SSRI treat- tathione along with metabolites in the purine and TRP ment. Blood samples were collected at these same time pathways, has been identified30. points. Pharmacometabolomics has also revealed that patients’ The HRSD17 was used to ascribe “response”—defined as metabolomic profiles (metabotypes), both prior to and at least 50% reduction in the total score from baseline to early during treatment, can inform treatment out- exit; “remission”—an exit HRSD17 score of 7 or less; and comes10,31. This approach has been applied to anti- “complete-non-response”—less than 30% reduction in the 32 33 39 hypertensive and antiplatelet therapies. We have used HRSD17 total score from baseline to exit . Genome-wide this approach to predict treatment outcomes and to association studies for plasma concentrations of the SSRIs identify specific metabolomic pathways that were changed and metabolite levels40 and for response41 in this trial in response to sertraline34,35 and to ketamine36, a pro- have been published previously. The trial was designed as mising agent for treatment-resistant depression. We have a parallel to the large National Institute of Mental Health also employed
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