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A Genome-Wide Association Study of Equine Metabolic Syndrome And

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A Genome-Wide Association Study of Equine Metabolic Syndrome And A GENOME-WIDE ASSOCIATION STUDY OF EQUINE METABOLIC SYNDROME AND PITUITARY PARS INTERMEDIA DYSFUNCTION A Thesis Presented to the Faculty of the Graduate School of Cornell University In Partial Fulfillment of the Requirements for the Degree of Master of Science by Cassandra Lois Streeter January 2012 © 2012 Cassandra Lois Streeter ABSTRACT Laminitis remains a poorly understood form of lameness in the horse despite being heavily researched. Endocrinopathic laminitis is an insidious form of laminitis that often results from insulin resistance (an indicator of Equine Metabolic Syndrome or EMS) or equine pituitary pars intermedia dysfunction (PPID). Both conditions occur in older horses with PPID having a later age of onset than EMS and horses are often characterized as having a “cresty” neck. EMS horses have persistent hyperinsulinemia while horses affected by PPID have elevated ACTH levels. Though no specific genetic predispositions have been identified for EMS or PPID, ponies are frequently observed to have both conditions. The objective of this study was to conduct a genome-wide association to identify candidate genes that may predispose individuals to EMS, PPID or both. A total of 65 horses, at least ten years old and of full or majority Arabian descent were divided into four categories based on previous endocrinology testing. Horses with ACTH levels greater than 70 pg/mL and insulin levels less than 40 uIU/mL were designated “PPID” while horses with insulin levels > 70 uIU/mL and ACTH levels less than 40 pg/mL were designated “EMS”. Horses with ACTH and insulin levels < 40 pg/mL or uIU/mL, respectively, were considered “Normal” and those with both levels > 70 were grouped into the fourth category termed “Both”. The Illumina Infinium® II Assay (Illumina Inc, San Diego, CA, USA) was performed on the EquineSNP 50 Genotyping BeadChip (Illumina Inc, San Diego, CA, USA) using DNA extracted from tail hair or blood samples. Data was analyzed using the PLINK v1.07 Whole genome association analysis toolset and JMP 8.0 (SAS Insitute Inc, Cary, NC, USA). Basic association (chi-square) tests were used to compare various groupings of disease cases and controls for a total of 15 qualitative associations. Quantitative associations were also performed for highest recorded ACTH (excluding August-October measurements) and insulin levels. An additional association was performed comparing horses with at least one episode of laminitis versus those with no recorded history of laminitis, regardless of disease category. SNPs with a missing genotype rate greater than 10% were excluded from analysis. SNPs in linkage disequilibrium (r2 > 0.99) were pruned to a single SNP per haplotype block in all graphs. Any SNPs exceeding the Bonferroni α threshold were mapped on the UCSC Genome Browser using the September 2007 assembly (EquCab2.0) of the horse genome. Candidate genes were identified within ~50 kb of each significant SNP. A total of 68 SNPs representing 59 different loci exceeded the significance threshold after correction for multiple testing. The most significant SNP, BIEC2-215377 (ECA 13) with a P-value of 4.34 e-7, was identified in the quantitative association with the highest ACTH level recorded for each individual. This SNP yielded one candidate gene, XPO6. Other SNPs that did not exceed the significance threshold but did vary from their expected P-value in a quantile-quantile plot include BIEC2-770354 (ECA3) and its neighboring SNP in linkage disequilibrium, BIEC2-770355 which yielded two candidate genes, FTO and ATP5H. This study resulted in the discovery of several good candidate genes that are worthy of fine mapping. However, epistatic effects may hamper the ability to identify all genetic predispositions. Improvements in diagnostic testing may allow for more specific classification of disease categories and refinement of the current data set. The present study is also limited by the relatively limited coverage of the Equine SNP50 chip. Expanded platforms with better coverage may allow for the discovery of more candidate genes. BIOGRAPHICAL SKETCH Cassy Streeter was born and raised in Syracuse, NY. She received her B.S. in Animal Science at Cornell University in January 2006. She is a Thoroughbred and Standardbred racing person. After this thesis she now considers herself an Arabian person and, after receiving many offers for free Arabians during the study, she may end up owning one in the future. iii This thesis is dedicated to all of the owners of the horses that participated in this study. iv ACKNOWLEDGMENTS I would like to thank my advisor, Dr. Samantha Brooks, as well as my other committee members, Dr. Yves Boisclair and Dr. Ned Place, for taking the time to review my thesis. I would also like to thank Beth Minnich and the Arabian Horse Foundation as well as Dr. Don Walsh and the Animal Health Foundation for their support of this project. Thank you to Barb Schanbacher and the Endocrinology lab for their help collecting DNA samples and health records. I extend my undying gratitude to Bethany for waking up at the crack of dawn in the middle of winter to help me draw blood from shaggy Arabians. My undergraduates, Elissa, Lauren, Carla, Brooke, Nicole, Rachel and Taylor, deserve a big thank you for testing my patience, supplying funny stories and making me a better person. It was a real pleasure passing on my knowledge and learning new things along with them. They remind me how fun science can really be. I’d like to express my sincere appreciation to all of the owners and horses who participated in this study. Their personal stories were a constant reminder of why this work like this needs to be done. I would also like to thank Jim Moran and Angelo LaBella for allowing me to be their “barn mascot” and feel like I actually own horses even though I don’t foot the bills. Finally, I’d like to thank my parents, sister and brother for all of their love and support throughout the years. v TABLE OF CONTENTS Biographical sketch iii Dedication iv Acknowledgements v List of Figures vii List of Abbreviations viii Chapter 1 – Review of Literature 1 Chapter 2 – Original Research 21 Appendix 46 vi LIST OF FIGURES Figure 2.1 Quantitative association of highest recorded ACTH value p 27 Figure 2.2 Quantitative association of highest recorded insulin value p 28 Figure 2.3 Qualitative association of hyperinsulinemia (B or M) vs. controls (N) p 29 Figure 2.4 Quantile-quantile plot of qualitative association of all disease groups (B, M and P) vs. controls (N) p 30 vii LIST OF ABBREVIATIONS α-MSH – alpha-melanocyte-stimulating hormone ACTH – adrenocorticotropic hormone AIRg – acute insulin response to glucose BCS – body condition score DST – dexamethasone suppression test ECD – equine Cushing’s Disease EMS – equine metabolic syndrome FSIGTT – frequently sampled intravenous glucose tolerance test POMC – proopiomelanocortin PPID – pituitary pars intermedia dysfunction SNP – single nucleotide polymorphism viii CHAPTER 1 Review of Literature Introduction Laminitis remains a poorly understood form of lameness in the horse despite being heavily researched. While many studies have focused on the pathophysiology of acute onset laminitis due to experimental models like carbohydrate overload [1] and black walnut extract [2], attention is now being given to an insidious form of laminitis referred to as endocrinopathic laminitis [3]. Endocrinopathic laminitis is often a result of insulin resistance (associated with equine metabolic syndrome) or equine pituitary pars intermedia dysfunction (aka equine Cushing’s Disease). Both equine metabolic syndrome and pituitary pars intermedia dysfunction show similar phenotypes and can be difficult to distinguish without diagnostic testing. There are currently no cures for either condition, though some management and drug therapies are available. Equine Metabolic Syndrome The term equine metabolic syndrome (EMS) was first coined in 2002 by Johnson to describe horses that show signs of chronic/previous laminitis in the absence of acute triggers such as carbohydrate overload or colic [4]. These horses are often obese and referred to as “easy keepers” because of their ability to maintain body weight with decreased caloric intake. The core characteristics of a horse affected by EMS are increased adiposity (often regional), insulin resistance (characterized by hyperinsulinemia) and a predisposition towards laminitis [4]. Johnson noted the age of onset to range from 8-18 years [4], though cases have occasionally appeared outside of this age range. Obesity in horses is not as clearly defined as the body mass index designed for humans [5] because the popular Henneke scale [6] does not account for regional adiposity. While generalized obesity is often linked to insulin resistance in horses [7], 1 regional adiposity is now gaining recognition as a high risk factor for insulin resistance [8, 9]. The regional adiposity often seen in an EMS affected horse manifests as a “cresty neck” in which fat accumulates along the nuchal ligament on the dorsal side of the neck. Larger mean neck circumference has previously been shown to be associated with lowered glucose tolerance [8].This regional adiposity is comparable to the accumulation of fat around the waist in humans, a characteristic that is a risk factor for human metabolic syndrome [10]. Since the Henneke scale does not properly address this issue, an objective scoring system for quantifying the relative size of the crest of the neck has been developed with scores ranging from 0 to 5 [11]. Horses with scores greater than or equal to 3 are classified as having an excessively cresty neck. Horses in this category also tend to have regional fat accumulation in the supraorbital fat pads, tail head and the shoulder area [4]. Hyperinsulinemia, an indication of insulin resistance [8, 12, 13], is a key characteristic of EMS [4]. While the plasma insulin concentration in fasted, healthy horses is often less than 20 uIU/mL [14], insulin resistant horses can have levels in excess of 70 uIU/mL [15].
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