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IV Spanish Portuguese Biophysical Congress Book of Abstracts IV Spanish Portuguese Biophysical Congress Zaragoza, Spain July 7-10, 2010 IV SPanISh PortugueSe BIophysIcal congress Table of contents Program .........................................................................................................V Committees ..................................................................................................IX Plenary Lectures ........................................................................................... 1 Bruker and SBe awards................................................................................. 9 Invited Speakers .........................................................................................15 Short oral Presentations ............................................................................51 Posters .........................................................................................................89 Companies .................................................................................................269 List of Contributors in alphabetical order ...............................................273 List of Participants in alphabetical order ................................................295 III IV IV SPanISh PortugueSe BIophysIcal congress Program 7th of July (Wednesday) Afternoon session 16:00-18:30 Registration 18:30-19:00 Congress Opening 19:00-20:00 Opening Lecture: Josep rizo (U. Texas, USA) 20:00-21:00 Welcome reception 8th of July (Thursday) Morning session SIMPOSIUM I SIMPOSIUM II Protein stability, folding and dynamics Membrane biophysics chairs: chairs: cláudio M gomes (ITQB, UNL, Oeiras) Maria João Moreno (FCTUC, Coimbra) Margarita Menéndez (IQFR, CSIC, Jesús pérez-gil (UCM, Madrid) Madrid) 09:00-09:30 Teresa pinheiro sergi garcía-Manyes Dep. Biol. Sciences. U. Warwick (UK) U. Columbia (USA) 09:30-10:00 Víctor Muñoz Margarida Bastos CIB, Madrid FCUP, Oporto 10:00-10:30 J.M. sánchez ruiz Francisco Monroy Dep. Química-Física. U. Granada UCM, Madrid 10:30-11:00 Coffee break 11:00-11:15 Hugo Botelho Beatriz Apellaniz 11:15-11:30 Fernando Diez-García Ana P. Martins 11:30-11:45 Ana Cristina Sotomayor Pérez Oliveira Costa 12:00-12:45 Plenary lecture: Marta Bruix (IQFR-CSIC, Madrid) V 12:50 Assembly of the SBE Board Afternoon session SIMPOSIUM III SIMPOSIUM IV Biophysical analysis of proteins and Bioenergetics macromolecular assemblies chairs: chairs: andreia Fernandes (IBMC, Porto) María João romão (FCT-UNL) Milagros Medina (U. Zaragoza, Zaragoza) o. llorca (CIB, Madrid) 14:30-15:00 luis loura Inês cardoso pereira U. Coimbra ITQB-UNL, Oeiras 15:00-15:30 José ruiz castón eduardo rial CNB-CSIC CIB, Madrid 15:30-16:00 guillermo Montoya Miguel angel de la rosa CNIO, Madrid U. Sevilla 16:00-17:30 Coffee break & poster session 17:30-17:45 Inmaculada Pérez-Dorado Patricia Ferreira Neila 17:45-18:00 M. Angeles Jiménez. Paulo Gameiro 18:00-18:15 Esther Ortega Portero Jesus I Martinez 18:30-18:50 Plenary lecture: SBE Award (pau Bernadó) 18:50-19:10 Plenary lecture: Bruker Award (Modesto orozco) 19:10-19:30 Plenary lecture: Bruker Award (José luis rodríguez arrondo) 19:30 SBE general assembly/SPBf general assembly 9th of July (Friday) Morning session SIMPOSIUM V SIMPOSIUM VI advances in biophysical experimental Ion channel and transporters methods chairs: chairs: antonio Ferrer-Montiel (UMH, Elche) José rino (IMM - Fac. Medicina U. Lisboa) graça soveral (FF, U. Lisboa) germán rivas (CIB. CSIC, Madrid) 09:00-09:30 Marisela Vélez Teresa giráldez I. Catálisis, CSIC, Madrid U.Inves., Hospital Ntra Sra Candelaria(Tenerife) 09:30-10:00 ana garcía-sáez Fátima Martel Biophysic/BIOTEC; Dresden (Germany) U. Oporto 10:00-10:30 Marcelo nöllman Vicente aguilella CBS CNRS-MontPellier (France) U. Jaime I, Castellón VI IV SPanISh PortugueSe BIophysIcal congress 10:30-11:00 Coffee break 11:00-11:15 Adriana Martin de Aguilera M. P. Lostao 11:15-11:30 Sebastian Raja Joanna Bielanska 11:30-11:45 Rocio Esquembre Tomé Barbara Tavares 12:00-12:45 Plenary lecture: Miguel Teixeira (ITBQ, Universidade Nova de Lisboa) Afternoon session SIMPOSIUM VII SIMPOSIUM VIII Biological Physics Cell Biophysics chairs: chairs: rui Dilão (IST, U. Técnica de Lisboa) Jorge carneiro Félix ritort (U. Barcelona) (I. Gulbenkian de Ciência, Oeiras) lucia Tabares (U. de Sevilla) 14:30-15:00 Jordi garcía-ojalvo Xavier Trepat UPC, Barcelona IBC-FM, Universitat de Barcelona 15:00-15:30 leonor cruzeiro Filipa alves U. Algarve, Faro I. Gulbenkian de Ciência, Oeiras 15:30-16:00 Daniel navajas Maria garcía-parajo U. Barcelona IBEC, Barcelona 16:00-17:30 Coffee break & poster session 17:30-17:45 Armindo Salvador Jaume Casademunt 17:45-18:00 Marta Ibañes Margarita Segovia Roldan 18:00-18:15 Alessandro Fiasconaro Rocio Ruiz Laza 18:30-19:30 Plenary lecture-Comité IUPAB: carlos Bustamante (U. California, Berkeley, USA) 21:00 Congress dinner 10th of July (Saturday) Morning session SIMPOSIUM IX SIMPOSIUM X single-molecule biophysics and nano- advances in biophysical computation technology and modelling chairs: chairs: yann astier (ITQB, U. Nova de Lisboa) Modesto orozco (IRB, Barcelona) ricardo Ibarra (INA, U. Zaragoza) armindo salvador (CNC, U. Coimbra) 09:00-09:30 christophe Vieu Victor guallar LAAS-CNRS, Toulouse (France) BSC, Barcelona VII 09:30-10:00 José Ignacio pascual Xavier Daura Berlín (Germany) ICREA-UAB, Barcelona 10:00-10:30 Mariano carrión-Vázquez paulo Martel I. Cajal-CSIC, Madrid FST. U Algarve, Faro 10:30-11:00 Coffee break 11:00-11:15 Anabel Lostao José García de la Torre 11:15-11:30 Lara H Moleiro Federico Morán 11:30-11:45 Cristiano Bizarro Inma Rodriguez Cantalapiedra 12:00-13:00 Closing lecture: chris M. Dobson (U. Cambridge, UK) Closing VIII IV SPanISh PortugueSe BIophysIcal congress Committees Chairman Javier Sancho (BIFI, U. Zaragoza, Spain) organizing Committee Alicia Alonso (U. País Vasco, Bilbao) Pierpaolo Bruscolini (BIFI, Zaragoza) Fernando Falo (BIFI, U. Zaragoza) Andreia S. Fernandes (IBMC, Porto) Carlos Gómez-Moreno (INA, U. Zaragoza) Milagros Medina (BIFI, U. Zaragoza) Manuela M. Pereira (ITQB, Oeiras) Jesús Pérez Gil (U. Complutense, Madrid) Germán Rivas (CIB-CSIC, Madrid) Javier Sancho (BIFI, U. Zaragoza) Nuno C. Santos (IMM, Lisboa) Adrián Velázquez-Campoy (BIFI, Zaragoza) Scientific Committee Alicia Alonso (EHU, Bilbao) Peter Eaton (FC, Porto) Juan Carmelo Gómez-Fernandez (U. Murcia) Antonio Ferrer Montiel (UMH, Elche) Manuela M. Pereira (ITQB, Oeiras) Manuel Prieto (U. Tecnica Lisboa, Lisboa) Nuno C. Santos (U. Lisboa) Javier Sancho (U. Zaragoza) Cláudio M. Soares (ITQB, Oeiras) Lucía Tabares (U. Sevilla) Conference Secretary Beatriz Antolí Isabel Vidal IX IV SPanISh PortugueSe BIophysIcal congress Plenary Lectures IV SPanISh PortugueSe BIophysIcal congress plenary lectures 1/new Insights from old proteins: Structure, Folding, Stability and Interactions of ribonucleases Studied by nMr ................................................ 4 Marta Bruix 2/the folding cooperativity of a protein is controlled by the topology of its polypeptide chain ............................................................................................... 5 Carlos Bustamante, Elizabeth Shank, Ciro Cecconi, Jesse Dill and Susan Marqusee 3/Life on the edge: the generic nature of Protein Misfolding Disorders ....... 6 Christopher M. Dobson 4/Structural Insights into the Mechanisms of neurotransmitter release and Information Processing in the Brain .................................................................. 7 Cong Ma, Wei Li, Yibin Xu, Lijing Su, Josep Rizo 5/reductive elimination of reactive oxygen Species: Structural and Functional Insights .............................................................................................. 8 Miguel Teixeira 3 plenary lectures 1 new Insights from old proteins: Structure, Folding, Stability and Interactions of ribonucleases Studied by nMr Marta Bruix1 1Instituto de Química Física Rocasolano, Serrano 119, 28006 Madrid, Spain. Ribonucleases are interesting proteins and besides sharing a common ribonucleolytic activity, RNases play a number of different and crucial biological functions. Due to their small size and high stability, RNases have been extensively characterised and widely used as models in many biophysical studies. We have long employed NMR to determine the 3D structure, stability, folding, electrostatics, internal dynamics and intermolecular interactions of RNases, and used these data to gain insights into biological activities. Recently, modern recombinant expression methods have spurred the ready production of labelled human RNases opening the possibility to their study by NMR. Here, I will present our recent NMR results on human RNase 1 (HP-RNase) [1] and RNase 3 (ECP) [2,3] and also on bovine pancreatic RNase A [4,5]. On the bases of these data, the structure-activity relationship for RNase 1 will be described with especial emphasis on the structural factors affecting its binding with the specific RNase inhibitor (hcRI) [1]. RNase 3 is an Arg- and Pro- rich cytotoxic RNase implicated in asthma and the immune defence against parasites. Our results have shed light onto the origin of its extremely high conformational stability [2], and the interactions with membranes and hepa- rin mimetics [3]. Our results show that ECP binds to membrane and heparin simultaneously suggesting pathways for an efficient membrane internationalization. Also, we have shown that the N-terminal fragment (ECP 1-45) retains the capacity to bind membrane and heparin mimetics, thus neither the ECP 3D structure nor its high
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