Embryonic Stem Cell Research Michael Phan & Jeniene Hassan
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Stem Cells Cloning Prons & Cons
& Trans g ge in n n e s o l i s C Cloning and Transgenesis Kalodimou, Clon Transgen 2014, 3:3 ISSN: 2168-9849 DOI: 10.4172/2168-9849.1000127 Short Communication Open Access Stem Cells Cloning Prons & Cons Vasiliki E Kalodimou* Research and Regenerative Medicine Department, IASO Maternity Hospital, Athens, Greece *Corresponding author: Vasiliki E Kalodimou, Head of Flow Cytometry-Research and Regenerative Medicine Department, IASO Maternity Hospital, Athens, Greece, Tel: 0030-210-618-5; E-mail: [email protected] Rec date: May 16, 2014; Acc date: Jun 30, 2014; Pub date: July 2, 2014 Copyright: © 2014 Kalodimou VE. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Stem Cell Cloning the target cell. In principle, this may be dangerous, because the gene therapy vector can potentially modify the activity of neighboring genes Over the last several decades, the ideas of both stem cell research (positively or negatively) in close proximity to the insertion site or and cloning have received significantly more attention than ever even inactivate host genes by integrating into them [6]. These before; currently there are 23 open/completed clinical trials, together phenomena may contribute to the malignant transformation of the with a great deal of controversy. This new advances in medical targeted cells, ultimately resulting in cancer. Another major limitation technology had provide scientific with a new view of potential to help of using adult stem cells is that it is relatively difficult to maintain the people suffering from various diseases as well as to help them recover stem cell state during ex vivo manipulations. -
Human Cloning: Must We Sacrifice Medical Research in the Name of a Total Ban?
S. HRG. 107–812 HUMAN CLONING: MUST WE SACRIFICE MEDICAL RESEARCH IN THE NAME OF A TOTAL BAN? HEARING BEFORE THE COMMITTEE ON THE JUDICIARY UNITED STATES SENATE ONE HUNDRED SEVENTH CONGRESS SECOND SESSION FEBRUARY 5, 2002 Serial No. J–107–55 Printed for the use of the Committee on the Judiciary ( U.S. GOVERNMENT PRINTING OFFICE 83–684 PDF WASHINGTON : 2002 For sale by the Superintendent of Documents, U.S. Government Printing Office Internet: bookstore.gpo.gov Phone: toll free (866) 512–1800; DC area (202) 512–1800 Fax: (202) 512–2250 Mail: Stop SSOP, Washington, DC 20402–0001 VerDate Feb 1 2002 09:13 Jan 16, 2003 Jkt 083684 PO 00000 Frm 00001 Fmt 5011 Sfmt 5011 C:\HEARINGS\83684.TXT SJUD4 PsN: CMORC COMMITTEE ON THE JUDICIARY PATRICK J. LEAHY, Vermont, Chairman EDWARD M. KENNEDY, Massachusetts ORRIN G. HATCH, Utah JOSEPH R. BIDEN, JR., Delaware STROM THURMOND, South Carolina HERBERT KOHL, Wisconsin CHARLES E. GRASSLEY, Iowa DIANNE FEINSTEIN, California ARLEN SPECTER, Pennsylvania RUSSELL D. FEINGOLD, Wisconsin JON KYL, Arizona CHARLES E. SCHUMER, New York MIKE DEWINE, Ohio RICHARD J. DURBIN, Illinois JEFF SESSIONS, Alabama MARIA CANTWELL, Washington SAM BROWNBACK, Kansas JOHN EDWARDS, North Carolina MITCH MCCONNELL, Kentucky BRUCE A. COHEN, Majority Chief Counsel and Staff Director SHARON PROST, Minority Chief Counsel MAKAN DELRAHIM, Minority Staff Director (II) VerDate Feb 1 2002 09:13 Jan 16, 2003 Jkt 083684 PO 00000 Frm 00002 Fmt 5904 Sfmt 5904 C:\HEARINGS\83684.TXT SJUD4 PsN: CMORC C O N T E N T S STATEMENTS OF COMMITTEE MEMBERS Page Brownback, Hon. -
Characterization of Embryonic Stem Cell-Differentiated Cells As Mesenchymal Stem Cells
The University of Southern Mississippi The Aquila Digital Community Honors Theses Honors College Fall 12-2015 Characterization of Embryonic Stem Cell-Differentiated Cells as Mesenchymal Stem Cells Rachael N. Kuehn University of Southern Mississippi Follow this and additional works at: https://aquila.usm.edu/honors_theses Part of the Cell Biology Commons Recommended Citation Kuehn, Rachael N., "Characterization of Embryonic Stem Cell-Differentiated Cells as Mesenchymal Stem Cells" (2015). Honors Theses. 349. https://aquila.usm.edu/honors_theses/349 This Honors College Thesis is brought to you for free and open access by the Honors College at The Aquila Digital Community. It has been accepted for inclusion in Honors Theses by an authorized administrator of The Aquila Digital Community. For more information, please contact [email protected]. The University of Southern Mississippi Characterization of Embryonic Stem Cell-Differentiated Cells as Mesenchymal Stem Cells by Rachael Nicole Kuehn A Thesis Submitted to the Honors College of The University of Southern Mississippi in Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in the Department of Biological Sciences December 2015 ii Approved by ______________________________ Yanlin Guo, Ph.D., Thesis Adviser Professor of Biological Sciences ______________________________ Shiao Y. Wang, Ph.D., Chair Department of Biological Sciences ______________________________ Ellen Weinauer, Ph.D., Dean Honors College iii ABSTRACT Embryonic stem cells (ESCs), due to their ability to differentiate into different cell types while still maintaining a high proliferation capacity, have been considered as a potential cell source in regenerative medicine. However, current ESC differentiation methods are low yielding and create heterogeneous cell populations. -
Multipotent Cell Types in Primary Fibroblast Cell Lines Used to Clone Pigs Using Somatic Cell Nuclear Transfer Sharon J
& Trans Harrison et al., Clon Transgen 2015, 4:2 g ge in n n e DOI: 10.4172/2168-9849.1000136 s o l i s C Cloning & Transgenesis ISSN: 2168-9849 Research Article Open Access Multipotent Cell Types in Primary Fibroblast Cell Lines Used to Clone Pigs using Somatic Cell Nuclear Transfer Sharon J. Harrison, Luke F.S. Beebe, Ivan Vassiliev, Stephen M. McIlfatrick and Mark B. Nottle* Reproductive Biotechnology Group, Centre for Stem Cell Research, Robinson Institute School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, 5005, Australia Abstract We have previously demonstrated that the use of porcine mesenchymal stem cells (MSCs) isolated from the bone marrow can increase the proportion of somatic cell nuclear transfer (SCNT) embryos that develop to the blastocyst stage compared with adult fibroblasts obtained from the same animal. The aim of the present study was to determine if MSCs are also present in primary cultures of adult fibroblasts which are commonly used for cloning live animals. To do this we chose a primary culture of adult fibroblasts that we had previously used to clone pigs. Single cell clones were isolated using low-density plating. After seven days of culture 63% of colonies displayed typical fibroblast morphology, while the remainder appeared cobblestone-like in appearance. Two of the 57 clones that displayed fibroblast morphology differentiated into adipocytes but not chondrocytes or osteocytes (uni-potent clones). Three of the 33 cobblestone-like clones differentiated into chondrocytes only, while 3 differentiated into adipocytes and chondrocytes but not osteocytes (bi-potent clones). One of the bi-potent cobblestone-like clones was then used for SCNT and in vitro development compared with a fibroblast-like clone which did not differentiate. -
Boosting the Cellular Potency of Embryonic Stem Cells by Spliceosome Targeting ✉ Wilfried A
Signal Transduction and Targeted Therapy www.nature.com/sigtrans RESEARCH HIGHLIGHT OPEN Boosting the cellular potency of embryonic stem cells by spliceosome targeting ✉ Wilfried A. Kues1 Signal Transduction and Targeted Therapy (2021) 6:324; https://doi.org/10.1038/s41392-021-00743-9 In recent work published in Cell, Shen et al.1 identified transfected ES cells with short interfering RNAs against different spliceosome inhibition in embryonic stem (ES) cells as a key spliceosome transcripts (the spliceosome consisted of 5 core and mechanism for the transition from pluri- to totipotency. Spliceo- several cofactor subunits, here 14 transcripts were targeted), some inhibition, achieved by RNA interference or the chemical respectively. Transient repression of 10 of the 14 splicing factors inhibitor pladienolide B, may gain widespread relevance to the resulted in ES cells, which maintained the typical colony culture of totipotent ES cells, in vitro differentiation of extra- morphology, however, pluripotent marker genes—Oct4 (Pou5f1), embryonal tissue and organoids, translation to the maintenance of Nanog, Sox2, Zfp42 and others—became down-regulated, at the pluripotent cells of other mammal species, including humans, and same time marker genes of totipotency—particularly Zscan4s and a better molecular understanding of cellular potency in stem cells MERVL—were up-regulated. Zscan4s (Zink finger and SCAN and cancer. domain containing 4) is a transcription factor and MERVL (murine The first successful isolation and maintenance of ES derived endogenous retrovirus L) an endogenous retrovirus with a usually fi 1234567890();,: from the inner cell mass (ICM) of murine blastocyst stages was restricted expression to 2-cell embryos. These results were veri ed described in 1981,2 and since then acted as game changer for by supplementing the culture medium with pladienolide B, a genetic studies in this mammalian model organism. -
A Concise Review on the Classification and Nomenclature of Stem Cells Kök Hücrelerinin S›N›Fland›R›Lmas› Ve Isimlendirilmesine Iliflkin K›Sa Bir Derleme
Review 57 A concise review on the classification and nomenclature of stem cells Kök hücrelerinin s›n›fland›r›lmas› ve isimlendirilmesine iliflkin k›sa bir derleme Alp Can Ankara University Medical School, Department of Histology and Embryology, Ankara, Turkey Abstract Stem cell biology and regenerative medicine is a relatively young field. However, in recent years there has been a tremen- dous interest in stem cells possibly due to their therapeutic potential in disease states. As a classical definition, a stem cell is an undifferentiated cell that can produce daughter cells that can either remain a stem cell in a process called self-renew- al, or commit to a specific cell type via the initiation of a differentiation pathway leading to the production of mature progeny cells. Despite this acknowledged definition, the classification of stem cells has been a perplexing notion that may often raise misconception even among stem cell biologists. Therefore, the aim of this brief review is to give a conceptual approach to classifying the stem cells beginning from the early morula stage totipotent embryonic stem cells to the unipotent tissue-resident adult stem cells, also called tissue-specific stem cells. (Turk J Hematol 2008; 25: 57-9) Key words: Stem cells, embryonic stem cells, tissue-specific stem cells, classification, progeny. Özet Kök hücresi biyolojisi ve onar›msal t›p görece yeni alanlard›r. Buna karfl›n, son y›llarda çeflitli hastal›klarda tedavi amac›yla kullan›labilme potansiyelleri nedeniyle kök hücrelerine ola¤anüstü bir ilgi art›fl› vard›r. Klasik tan›m›yla kök hücresi, kendini yenileme ad› verilen mekanizmayla farkl›laflmadan kendini ço¤altan veya bir dizi farkl›laflma aflamas›ndan geçerek olgun hücrelere dönüflebilen hücrelerdir. -
Ethical Challenges in Organoid Use
Article Ethical Challenges in Organoid Use Vasiliki Mollaki Hellenic National Bioethics Commission, PC 10674 Athens, Greece; [email protected] Abstract: Organoids hold great promises for numerous applications in biomedicine and biotech- nology. Despite its potential in science, organoid technology poses complex ethical challenges that may hinder any future benefits for patients and society. This study aims to analyze the multifaceted ethical issues raised by organoids and recommend measures that must be taken at various levels to ensure the ethical use and application of this technology. Organoid technology raises several serious ethics issues related to the source of stem cells for organoid creation, informed consent and privacy of cell donors, the moral and legal status of organoids, the potential acquisition of human “characteristics or qualities”, use of gene editing, creation of chimeras, organoid transplantation, commercialization and patentability, issues of equity in the resulting treatments, potential misuse and dual use issues and long-term storage in biobanks. Existing guidelines and regulatory frameworks that are applicable to organoids are also discussed. It is concluded that despite the serious ethical challenges posed by organoid use and biobanking, we have a moral obligation to support organoid research and ensure that we do not lose any of the potential benefits that organoids offer. In this direction, a four-step approach is recommended, which includes existing regulations and guidelines, special regulatory provisions that may be needed, public engagement and continuous monitoring of the rapid advancements in the field. This approach may help maximize the biomedical and social benefits of organoid technology and contribute to future governance models in organoid technology. -
Replication Study: Wnt Activity Defines Colon Cancer Stem Cells and Is
REPLICATION STUDY Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment Anthony Essex1, Javier Pineda1, Grishma Acharya2, Hong Xin2, James Evans1, Reproducibility Project: Cancer Biology* 1PhenoVista Biosciences, San Diego, United States; 2Explora BioLabs Inc, San Diego, United States Abstract As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Evans et al., 2015), that described how we intended to replicate selected experiments from the paper ‘Wnt activity defines colon cancer stem cells and is regulated by the microenvironment’ (Vermeulen et al., 2010). Here, we report the results. Using three independent primary spheroidal colon cancer cultures that expressed a Wnt reporter construct we observed high Wnt activity was associated with the cell surface markers CD133, CD166, and CD29, but not CD24 and CD44, while the original study found all five markers were correlated with high Wnt activity (Figure 2F; Vermeulen et al., 2010). Clonogenicity was highest in cells with high Wnt activity and clonogenic potential of cells with low Wnt activity were increased by myofibroblast-secreted factors, including HGF. While the effects were in the same direction as the original study (Figure 6D; Vermeulen et al., 2010) whether statistical significance was reached among the different conditions varied. When tested in vivo, we did not find a difference in tumorigenicity between high and low Wnt activity, while the original study found cells with high Wnt activity were more effective in inducing tumors (Figure 7E; Vermeulen et al., 2010). Tumorigenicity, however, was increased with *For correspondence: myofibroblast-secreted factors, which was in the same direction as the original study (Figure 7E; [email protected]; Vermeulen et al., 2010), but not statistically significant. -
Targeting Chemoresistant Colorectal Cancer Via Systemic Administration of a BMP7 Variant
Oncogene (2020) 39:987–1003 https://doi.org/10.1038/s41388-019-1047-4 ARTICLE Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant 1 1 1 1 1 Veronica Veschi ● Laura R. Mangiapane ● Annalisa Nicotra ● Simone Di Franco ● Emanuela Scavo ● 1 1 2 1 1 1 Tiziana Apuzzo ● Davide S. Sardina ● Micol Fiori ● Antonina Benfante ● Maria L. Colorito ● Gianfranco Cocorullo ● 3,4 1 5 5 6 Felice Giuliante ● Calogero Cipolla ● Giuseppe Pistone ● Maria Rita Bongiorno ● Aroldo Rizzo ● 7 7 7 7 5 3,4 Courtney M. Tate ● Xiaohua Wu ● Scott Rowlinson ● Louis F. Stancato ● Matilde Todaro ● Ruggero De Maria ● Giorgio Stassi1 Received: 10 April 2019 / Revised: 23 September 2019 / Accepted: 25 September 2019 / Published online: 7 October 2019 © The Author(s) 2019. This article is published with open access Abstract Despite intense research and clinical efforts, patients affected by advanced colorectal cancer (CRC) have still a poor prognosis. The discovery of colorectal (CR) cancer stem cell (CSC) as the cell compartment responsible for tumor initiation and propagation may provide new opportunities for the development of new therapeutic strategies. Given the reduced 1234567890();,: 1234567890();,: sensitivity of CR-CSCs to chemotherapy and the ability of bone morphogenetic proteins (BMP) to promote colonic stem cell differentiation, we aimed to investigate whether an enhanced variant of BMP7 (BMP7v) could sensitize to chemotherapy- resistant CRC cells and tumors. Thirty-five primary human cultures enriched in CR-CSCs, including four from chemoresistant metastatic lesions, were used for in vitro studies and to generate CR-CSC-based mouse avatars to evaluate tumor growth and progression upon treatment with BMP7v alone or in combination with standard therapy or PI3K inhibitors. -
Electrophysiology Read-Out Tools for Brain-On-Chip Biotechnology
micromachines Review Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology Csaba Forro 1,2,†, Davide Caron 3,† , Gian Nicola Angotzi 4,†, Vincenzo Gallo 3, Luca Berdondini 4 , Francesca Santoro 1 , Gemma Palazzolo 3,* and Gabriella Panuccio 3,* 1 Tissue Electronics, Fondazione Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci, 53-80125 Naples, Italy; [email protected] (C.F.); [email protected] (F.S.) 2 Department of Chemistry, Stanford University, Stanford, CA 94305, USA 3 Enhanced Regenerative Medicine, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy; [email protected] (D.C.); [email protected] (V.G.) 4 Microtechnology for Neuroelectronics, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy; [email protected] (G.N.A.); [email protected] (L.B.) * Correspondence: [email protected] (G.P.); [email protected] (G.P.); Tel.: +39-010-2896-884 (G.P.); +39-010-2896-493 (G.P.) † These authors contributed equally to this paper. Abstract: Brain-on-Chip (BoC) biotechnology is emerging as a promising tool for biomedical and pharmaceutical research applied to the neurosciences. At the convergence between lab-on-chip and cell biology, BoC couples in vitro three-dimensional brain-like systems to an engineered microfluidics platform designed to provide an in vivo-like extrinsic microenvironment with the aim of replicating tissue- or organ-level physiological functions. BoC therefore offers the advantage of an in vitro repro- duction of brain structures that is more faithful to the native correlate than what is obtained with conventional cell culture techniques. -
Telomere Shortening and Apoptosis in Telomerase-Inhibited Human Tumor Cells
Downloaded from genesdev.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Telomere shortening and apoptosis in telomerase-inhibited human tumor cells Xiaoling Zhang,1 Vernon Mar,1 Wen Zhou,1 Lea Harrington,2 and Murray O. Robinson1,3 1Department of Cancer Biology, Amgen, Thousand Oaks, California 91320 USA; 2Amgen Institute/Ontario Cancer Institute, Toronto, Ontario M5G2C1 Canada Despite a strong correlation between telomerase activity and malignancy, the outcome of telomerase inhibition in human tumor cells has not been examined. Here, we have addressed the role of telomerase activity in the proliferation of human tumor and immortal cells by inhibiting TERT function. Inducible dominant-negative mutants of hTERT dramatically reduced the level of endogenous telomerase activity in tumor cell lines. Clones with short telomeres continued to divide, then exhibited an increase in abnormal mitoses followed by massive apoptosis leading to the loss of the entire population. This cell death was telomere-length dependent, as cells with long telomeres were viable but exhibited telomere shortening at a rate similar to that of mortal cells. It appears that telomerase inhibition in cells with short telomeres lead to chromosomal damage, which in turn trigger apoptotic cell death. These results provide the first direct evidence that telomerase is required for the maintenance of human tumor and immortal cell viability, and suggest that tumors with short telomeres may be effectively and rapidly killed following telomerase inhibition. [Key Words: TERT; telomere; dominant negative; proliferation; cancer] Received June 4, 1999; revised version accepted August 3, 1999. The termini of most eukaryotic chromosomes are com- TEP1 binds the telomerase RNA and associates with posed of terminal repeats called telomeres. -
My Life As a Clone
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Many Voices Poetry Slam & Creative Writing Collection Libraries at University of Nebraska-Lincoln Summer 8-6-2010 My life as a clone Rajkrishna Paul University of Nebraska at Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/manyvoicespoetry Part of the Creative Writing Commons, and the English Language and Literature Commons Paul, Rajkrishna, "My life as a clone" (2010). Many Voices Poetry Slam & Creative Writing Collection. 2. https://digitalcommons.unl.edu/manyvoicespoetry/2 This Article is brought to you for free and open access by the Libraries at University of Nebraska-Lincoln at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Many Voices Poetry Slam & Creative Writing Collection by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. MY LIFE AS A CLONE I am what, which I don’t know, of course, I am a living creature, but what exactly I am and how am I born, I didn’t got an idea. Whats my age? All of these are a hidden mystery. You might be thinking then what exactly do I want to tell you. Well I am a clone of Zehar, a scientist, he is the person who himself, cultivated a reproductive cloning on himself, to produce an exactly identical friend of his. Due to the extreme hardship for two rigorous, years, I am created, wonderfully by him. But there is some emotional sentimental part is related along my birth. I couldn’t able to accomplish true dream of Zehar, completely.