Efficiency of Plant Extract from Reynoutria Sachalinensis (Milsana

Eﬀiciency of plant extract from Reynoutria sachalinensis (Milsana) to control powdery mildew on tomato (Oïdium neolycopersici) Yannie Trottin-Caudal, Christine Fournier, Jean-Michel Leyre, Véronique Decognet, Philippe C. Nicot, Marc Bardin

To cite this version:

Yannie Trottin-Caudal, Christine Fournier, Jean-Michel Leyre, Véronique Decognet, Philippe C. Nicot, et al.. Eﬀiciency of plant extract from Reynoutria sachalinensis (Milsana) to control powdery mildew on tomato (Oïdium neolycopersici). Colloque International, Sep 2003, Avignon, France. hal-02764311

HAL Id: hal-02764311 https://hal.inrae.fr/hal-02764311 Submitted on 4 Jun 2020

HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés.

Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike| 4.0 International License biologique (p. 11-15). Presented at Colloque International, Avignon, FRA (2003-09-17 - 2003-09-19). Paris, FRA : CTIFL. Version postprint Efficiency ofplantextract fromReynoutria sachalinensis (Milsana) tocontrolpowdery mildewon Trottin-Caudal, Y.,Fournier, C.,Leyre, J.-M.,Decognet, V.,Nicot, P., Bardin,M. (2003). clés Mots traitementavec le a au obtenue étéfongicide équivalente et significative feuillage du protection une Enfin, l'oïdium. de d'attaque sévérité la de et l'incidence de réduction une présenté a plante de l'extrait d'application, 0,6 fréquences à (Anvil chimique référence une et eau témoin un avec comparaison en jours) 21 et 14 (7, d'application fréquences plusieurs comprennent étudiées modalités Les sur plante la de l'extrait avec culture différentes sur obtenus été ont encourageants résultats des et oïdiums les sur testés inte des sur essentiellement repose pathogène agent cet contre protection La abri. sous tomate sur aériennes fongiques Résumé Keywords appeared 7 every significantlydays be tonot differentfrom referencethe fungicide. disease. the of severity the reducing nu the reducing by plants of protection good a water with compared gave extract plant application, of were frequency the Whatever l/ha). 0.6 (Anvil, control fungicide obtained Results tested. been of control the have conditions greenhouse in grown 3 a during conducted tomato been on Experiments crops. various on disease giant the from knotweed extract plant a and studied been have methods biological Various disease. this control to used widely are Fungicides conditions. greenhouse in grown crops tomato on Abstract B.P.ResearchMaurice,94,Domaine84140Unit, St Montfavet, France. 1 Romiti Corinne Trottin Yannie ( mildew on tomato from extract plant of Efficiency intégré Protection ( tomato extr plant of Trottin tomato (Oïdium neolycopersici). In: Tomate sous abri.Protection intégrée. Agriculture

Ctifl, Centre de Balandran, B.P. 32, 30127 Bellegarde, France and France Bellegarde, 30127 32, B.P. Balandran, de Centre Ctifl, tomate durant trois années (1999 à 2001) pour étudier l'efficacité de cet extrait de plante.de extrait cet de l'efficacité étudier pour 2001) à (1999 années trois durant tomate - Caudal Y, Fournier C, Leyre JM, Decognet V, Nicot P, Bardin M, scientifique. Efficiencyscientifique.M, Bardin P, Nicot V, JM,Decognet Leyre FournierC, Y, Caudal

: L'oïdium : ïim neolycopersici Oïdium

:Tomate, by caused mildew Powdery : Tomato,: enura sachalinensis Reynoutria c from act O. neolycopersici O. 2 - Caudal ,Philippe Nicot e. Agriculturebiologique Oidiumneolycopersici Oidium neolycopersici Oidiumneolyco Odu neolycopersici) (Oidium 1 Comment citer cedocument: Reynoutria Crsie Fournier Christine , Reynoutria sachalinensis Reynoutria - year period (1999 period year vnin ciius Dféet pout booius n été ont biologiques produits Différents chimiques. rventions . n Ctifl In: ). 2 . Several frequencies of appl of frequencies Several . , Marc ,

enura sachalinensis Reynoutria

sachalinensis

persici

Mlaa hs hw a ih efficiency high a shown has (Milsana) Moreover, p Moreover, Oidium neolycopersici Oidium Bardin Ir, eds. Inra, , , protection, extraitbiologique, deplante,Milsana , , biological control, plant extract,biological control, , plant Milsana )

2003/09/17 1

Jean , -

à laà de7fréquence jours. 2

2001) to test the efficiency of this product for product this of efficiency the test to 2001) s lu ds rniax gns e maladies de agents principaux des l'un est

mber of leaflets attacked and especially by especially and attacked leaflets of mber Mlaa t cnrl odr mle on mildew powdery control to (Milsana) rotection due to the plant extract sprayed extract plant the to due rotection

(Milsana). Des essais ont été mis en place en mis été ont essais Des (Milsana). - ihl Leyre Michel rceig o te oae os abri. sous Tomate the of Proceedings - 19 ,

ication (7, 14 and 21 days) have days) 21 and 14 (7, ication

Avignon (FRA): is one of the principal disea principal the of one is Mlaa t cnrl powdery control to (Milsana) - rae pat ad ih a with and plants treated 1 Vrnqe Decognet Véronique , 2

INRA Plant Pathology Plant INRA

l/ha). A toutes les toutes A l/ha). 11

o oto this control to - 1 5.

ses

2 1 , biologique (p. 11-15). Presented at Colloque International, Avignon, FRA (2003-09-17 - 2003-09-19). Paris, FRA : CTIFL. Version postprint Efficiency ofplantextract fromReynoutria sachalinensis (Milsana) tocontrolpowdery mildewon Trottin-Caudal, Y.,Fournier, C.,Leyre, J.-M.,Decognet, V.,Nicot, P., Bardin,M. (2003). mildew powdery by infested area leaf mildewed the of controlmethodstheefficiencyof mildew).The withpowdery coveredleaf %of in (expressed estimation visual estima a was Severity on zone. treated based the in chosen randomly leaflets) terminal 3 the (on plant per leaves 3 and 4 respectively on recorded were severity and incidence disease 2001, plant per leaves two on recorded approxi (with was incidence disease 1999, In experiment. the the until of mildew powdery end of inoculation the after weekly monitored were infections Leaf ma the by recommended those were plants the on sprayed and Milsana 2000 of Doses and 2001). 1999 in VP2000 in (VP99 KG., Shaette Gebrüder by provided were extract plant the of (Anv fungicide a of that to compared was Milsana of efficiency The 75%. and 60% between comprised was powdery 22 and of April development the to A conducive used. were conditions mildew Climatic inoculated. was strains 2001 experiment for except experiment each for plants on inoculated o produced were Spores 2001 experiment in (except spores/ml with 7, at applied was extract plant The experimentswere Two evaluated. realised each year were replicate per plants Eight plants/m². 1.8 of density plant a with compartments) (60m² commercial a to to susceptible very cv. o control the on Milsana of effect The methodsand Material neolycopersici (1999 period 3 year a during conducted been have conditions greenhouse in grown tomato on Experiments al., et (Daayf resistance induced plant with associated be to supposed is cucumber on extract plant this of action of mode The 1990). Klingauf, and (Herger cucumber and begonia knotweed giant a Labbé, the and shown from (Bélanger extract mildews plant powdery A control to 2002). studied been have extracts, plant or method. this complicate pathogenbiolog Various the of strains resistant of emergence possible the and available offungicides number low the but disease this control to used widely are Fungicides principa the of one is 2001) species renamed recently the by caused mildew Powdery Introduction tomato (Oïdium neolycopersici). In: Tomate sous abri.Protection intégrée. Agriculture nufacturer nufacturer Trust O. neolycopercisi O.

in 1999 and cv. and 1999 in ih fiiny o oto pwey idw o vros rp icuig apple, including crops various on mildews powdery control to efficiency high -

23°C from May to August. Relative humidity (average over (average humidity Relative August. to May from 23°C mately 19 leaflets per leaf) randomly chosen in the treated zone. In 2000 and 2000 In zone. treated the in chosen randomly leaf) per leaflets 19 mately . i.e. il, 0.6 l/ha) and to that of a control (water control a of that to and l/ha) 0.6 il, ical methods, mainly based on the utilisation of antagonistic microorganisms antagonistic of utilisation the on based mainly methods, ical

situation. Treatments were arranged in a block design with four replicates four with design block a in arranged were Treatments situation. 0.25% and0.25% VP99 Milsanafor 1.3% VP2000.Milsanafor - O. neolycopersici O. 01 i odr o et h efcec o ti pout o control to product this of efficiency the test to order in 2001) eae eprtrs ee anand t 19 at maintained were temperatures verage . Powdery mildew was applied as a spore suspension containing 10 containing suspension spore a as applied was mildew Powdery .

Comment citer cedocument: n cotyledons except for experiment 2001 experiment for except cotyledons n Grace dsae o tmt cos rw i genos conditions. greenhouse in grown crops tomato on diseases l

in 2000 and 2001 in a glasshouse at Ctifl. Both cultivars are cultivars Both Ctifl. at glasshouse a in 2001 and 2000 in

14 or 21 or 14 . The cultural practices were kept as closely as possible as closely as kept were practices cultural The . f powdery mildew was evaluated on plants of tomato tomato of plants on evaluated was mildew powdery f - : nrdcin f otmntd oao plants). tomato contaminated of introduction 1: - day intervals starting 7 days before inoculation before days 7 starting intervals day

enura sachalinensis Reynoutria

- treated plants). Two formulations Two plants). treated Oidium neolycopersici Oidium - - 1. Strain Et1 (INRA) was (INRA) Et1 Strain 1. 0 C rm eray to February from C 20°

- 2 where a mix of 3 of mix a where 2 a 24 hours 24 a

(Milsana) has has (Milsana)

(Kiss

period) 1997). 1997). et al. et ted O. 2 4 - , biologique (p. 11-15). Presented at Colloque International, Avignon, FRA (2003-09-17 - 2003-09-19). Paris, FRA : CTIFL. Version postprint Efficiency ofplantextract fromReynoutria sachalinensis (Milsana) tocontrolpowdery mildewon Trottin-Caudal, Y.,Fournier, C.,Leyre, J.-M.,Decognet, V.,Nicot, P., Bardin,M. (2003). b a extract from of Incidence 1. Table significantly not and differentfrom referencethe fungicide. plan control the to relative days, 7 every oflast date the notation Moreover, (Table2). the due protection tothe plant extractsprayed at 2001 and 2000 in conducted tests 4 the in Milsana by provided was protection 80% to up int Whatever 1). (Figure application last the after days 30 to up least at with period, long a treated over effect an had plants product the that that and protected well indicated were Milsana plants on mildew powdery of development of Kinetics ofpowdery milde Severity 75%)toin all was reduced and (up plots treated(Table Milsana with 1). (inoculum plantscontrolon highleaflets wason mildew of powdery incidencethe notation,of date last day 1 between leaves on appeared d 20 and present) already probably plants control the on symptoms first The Incidence of powderymildew on tomato Results plantstreatedon controlrelativetoseverity the onuntreated plants. reducti the as computed index protectiona of calculation the by estimated was tomato (Oïdium neolycopersici). In: Tomate sous abri.Protection intégrée. Agriculture 2000 2000 1999 1999 Trial

standard errorofthestandard inoculation after of mildewdays on powdery plants

- - - - 2 1 2 1

3 10 1 20 (DAI) symptoms of appearance

Reynoutriasacchalinensis a

iim neolycopersici Oidium Comment citer cedocument: 14 14 18 14 (DAI) nt treatme last

mean

w ontomato w

36 31 21 50 (DAI) n notatio last

ays after artificial inoculation of inoculation artificial after ays

(Milsa

ts appeared to be statistically significant in all tests all in significant statistically be to appeared ts

99.7 99.7 84.4 90.2 76.4 (%) control the on leaflets diseased na na VP99).

n efes f oao rae wt te plant the with treated tomato of leaflets on

   

0.3 9.5 6.9 7.9

67.9 67.9 15.4 10.2 33.4 1.2 days7 Milsana plants treated on (%) leaflets diseased



 

0.6 ervals of treatments tested, tested, treatments of ervals

3.3 3.6

 O. neolycopersici O.

70.6 70.6 31.8 - - days 14 Milsana

 

7.3 14.1

on in severityin on

0.0 0.0 0.3 1.6 0. Anvil . At the At . 0    

0.0 0.3 0.8 0.0 3

biologique (p. 11-15). Presented at Colloque International, Avignon, FRA (2003-09-17 - 2003-09-19). Paris, FRA : CTIFL. Version postprint Efficiency ofplantextract fromReynoutria sachalinensis (Milsana) tocontrolpowdery mildewon Trottin-Caudal, Y.,Fournier, C.,Leyre, J.-M.,Decognet, V.,Nicot, P., Bardin,M. (2003). effect of systemic absence (dataproduct of this shown). not Milsana the of zone treated non the in observed were mildew th if even leaves the on 2000 (trial mildew powdery of incidence of reduction weak a with high be could index Protection Milsanathe on action Inference of a VP2000) Milsana tocontrol from extract plant the of Efficiency 2. Table beforeinoculation days after14 days inoculationand (+),andfungicide( the ( inoculation after days 7 and inoculation before days 7 days, 14 of inoculation artificial 2000 1:Protectionagainst withleaves Milsana ofFigure tomato powdery of mildew ingreenhouse tomato (Oïdium neolycopersici). In: Tomate sous abri.Protection intégrée. Agriculture

standard erro standard Trial 2001 2001 2000 2000

- Disease severity (% of mildewed leaf - 2 (VP99) and 2001 and (VP99) 2 2; Tables 1 and 2). This indicates that Milsana reduced the growth of powdery mildew powdery of growth the reduced Milsana that indicates This 2). and 1 Tables 2; area)

100

0 - - - -

0 2 1 2 1 2000

81.4 81.4 18.5 93.9 31.7 on (%) plants control area leaf Mildewed

5 - 2

Days after inoculation of powdery mildew powdery of inoculation after Days

rofthe mean

10    

5.4 9.6 3.7 15.0

O. neolycopersici O. Comment citer cedocument: - a 2 (VP2000). Four or five treatments were applied before and after and before applied were treatments five or Four (VP2000). 2

20 soe wr aray rsn. ial, ypos f powdery of symptoms Finally, present. already were spores e Oidiumneolycopersici 49 62 36 31 (DAI) n notatio last

35 99.8 99.8 99.9 93.3 94.8 days 7 Milsana Protection indexcontrol(%)relative to plants : water ( water :

   

0.0 0.0 0.9 1.9

enura sacchalinensis Reynoutria

on tomato. on Disease severity (% of mildewed leaf ev Milsana ),

96.7 96.7 99.0 81.6 94.3 days 14 Milsana area) 100 20 40 60 80 0 0 2001

    5

1.2 0.0 5.5 2.3 -

10 Days after inoculation of powdery mildew powdery of inoculation after Days

- ery 7 days ( days 7 ery treated plants, suggesting an suggesting plants, treated 15 93.9 93.9 days 21 Milsana ), Milsana every 21 days, 7 days, 21 every Milsana ), 20

 25

3.6 Mlaa P9 and VP99 (Milsana  30

 ), Milsana every every Milsana ), 35 ). 1.1 98.8 100 100 100 Anvil

 50

4 biologique (p. 11-15). Presented at Colloque International, Avignon, FRA (2003-09-17 - 2003-09-19). Paris, FRA : CTIFL. Version postprint Efficiency ofplantextract fromReynoutria sachalinensis (Milsana) tocontrolpowdery mildewon Trottin-Caudal, Y.,Fournier, C.,Leyre, J.-M.,Decognet, V.,Nicot, P., Bardin,M. (2003). N.E., Malathrakis, M., Bardin, I., Pascoe, S., Takamatsu, J.H., Cunnington, G.S., Saenz, R.T.A., Cook, L., Kiss, extractwith mildew fungi powdery Controlof 1990. Klinghauf,F. andG. Herger, of Induction 2000. R. R. Bélanger, and I. ElHadrami, R., Boulanger, M., Ongena, F., Daayf, chemicals: without mildews powdery of Control 2002. C. Labbé, & R.R. Bélanger, References wasstudy partlyfinancedThis anACTA(1999 by project Acknowledgements countriesbutit is some yet registerednot inFrance in industry agricultural the by used being currently is product This tomato. on management pest integrated an in Milsana include to order in products, or agents biocontrol other compat with the assess to needed be will work further But farming. organic in mildews powdery tomato of management the to contribute then could It leaves. on Milsana control to L13 strain Nicot Moreover tomato, on mildew powdery of agent other c the and pressure disease of levels the whatever consistent was product this of Efficiency 2001. to due mildew extract plant The Discussion tomato (Oïdium neolycopersici). In: Tomate sous abri.Protection intégrée. Agriculture ultivars of tomato tested. This product has also shown a good efficacy to control the control to efficacy good a shown also has product This tested. tomato of ultivars hnlc compou phenolic St APS Press, T.L.W.Eds) Carver and crops. A.J. Dik W.R., Bushnell horticultural R.R., (Bélanger treatise for comprehensive a mildews, alternatives biological and prophylactic taurica from extract Petsikos and world. of the Mycologicalparts Research 105:684 fungi, P Nicot, Landbouwwetenschappen. Rijksuniversiteit Gent 3a. V.55: knotweed, Ecology Chemical 26:1579 mildew powdery -

conclusion Oidium neolycopersici Oidium ) . IOBC/WPRS Bulletin 25:175 Bulletin . IOBC/WPRS C, ao Y ad osa, .. 01 Ietfcto o to odr mildew powdery two of Identification 2001. A.Y. Rossman, and Y. Sato, .C., O. neolycopersici O. et al. et enura sachalinensis Reynoutria

Markellou, E., Fanouraki, M.N., Kasselaki, A.M., Koumaki, C.M., Schmitt, A. Schmitt, C.M., Koumaki, A.M., Kasselaki, M.N., Fanouraki, E., Markellou, - Reynoutria sacchalinenesis Reynoutria Panayotarou, N. 2002. Efficacy of Milsana (VP1999), a formulated plant formulated a (VP1999), Milsana of Efficacy 2002. N. Panayotarou, from

(this book) have observed that the efficacy of of efficacy the that observed have book) (this - orts cinerea Botrytis Comment citer cedocument: infected plants with extracts of extracts with plants infected d i to utvr o ccme b tetet f elh and healthy of treatment by cucumber of cultivars two in nds . sacchalinensis R. - Paul, USA. Minnesota, Paul, Pp256 - 1593.

in the 6 greenhouses trials conducted between 1999 and 1999 between conducted trials greenhouses 6 the in

sp. nov. and nov. sp.

n oao a nt lee b te plcto of application the by altered not was tomato on -

178. Plgnca) Mddlne Vn e Faculteit De Van Mededelingen (Polygonaceae). infcnl rdcd h icdne f powdery of incidence the reduced significantly , against powdery mildew of tomato ( tomato of mildew powdery against ,

eelua taurica Leveillula O. lycopersici O. .

- 697. - Reynoutria sachalinensis Reynoutria 2001)

- 267. , infecting tomato in different in tomato infecting ,

(Malathrakis Microdochium In ibility of Milsana of ibility Te pow The : t al. et s of the giant ofthe s . Journal of Journal .

Leveillula dimerum 2002). , dery 5