DOCSLIB.ORG

Microbial Community Diversity in Freshwater Sponges from Lake Baikal/Protein Size in Three Domains of Life and Examples of Unusu

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Microbial Community Diversity in Freshwater Sponges from Lake Baikal/Protein Size in Three Domains of Life and Examples of Unusu Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2015 Microbial community diversity in freshwater sponges from Lake Baikal/Protein size in three domains of life and examples of unusually large proteins in Porifera: A review Katrina Agno Lutap Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Microbiology Commons, and the Molecular Biology Commons Recommended Citation Lutap, Katrina Agno, "Microbial community diversity in freshwater sponges from Lake Baikal/Protein size in three domains of life and examples of unusually large proteins in Porifera: A review" (2015). Graduate Theses and Dissertations. 16627. https://lib.dr.iastate.edu/etd/16627 This Thesis is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. ! Microbial!community!diversity!in!freshwater!sponges!from!Lake!Baikal! ! Protein!size!in!three!domains!of!life!and!examples!of!unusually!large!proteins!in! Porifera:!A!review! ! by! ! Katrina!A.!Lutap!! ! ! ! A!thesis!submitted!to!the!graduate!faculty! ! in!partial!fulfillment!of!the!requirements!for!the!degree!of! ! MASTER!OF!SCIENCE! ! ! Major:!Molecular,!Cellular,!and!Developmental!Biology! ! Program!of!Study!Committee:! Dennis!Lavrov,!Major!Professor! Stephan!Schneider! Amy!Toth! Edward!Yu! ! ! ! ! ! ! Iowa!State!University! ! Ames,!Iowa! ! 2015! ! ! Copyright!©!Katrina!A.!Lutap,!2015.!All!rights!reserved. ii TABLE&OF&CONTENTS& & & & & & & & & & & &&&Page& & ABSTRACT&& & & & & & & & & & &&&&&&&&&&iv& CHAPTER&1.&GENERAL&INTRODUCTION&& & & & & & &&&&&&&&&&&1& & Introduction&& & & & & & & & & &&&&&&&&&&&1& & Thesis&Organization&& & & & & & & & &&&&&&&&&&&2& & References&& & & & & & & & & &&&&&&&&&&&6& & CHAPTER&2.&MICROBIAL&COMMUNITY&DIVERSITY&IN&FRESHWATER&SPONGES&FROM&& LAKE&BAIKAL&& & & & & & & & & & &&&&&&&&&&9& & Abstract&& & & & & & & & & &&&&&&&&&&9& & Introduction&& & & & & & & & & &&&&&&&&10& & Materials&and&Methods&& & & & & & & &&&&&&&&16& & Results&& & & & & & & & & &&&&&&&&18& & Discussion&& & & & & & & & & &&&&&&&&25& & References&& & & & & & & & & &&&&&&&&31&& & Tables&and&Figures&& & & & & & & & &&&&&&&&38& & CHAPTER&3.&PROTEIN&SIZE&IN&THREE&DOMAINS&OF&LIFE&AND&EXAMPLES&OF&& UNUSUALLY&LARGE&PROTEINS&IN&PORIFERA:&A&REVIEW&& & & & &&&&&&&&45& & Introduction&& & & & & & & & & &&&&&&&&45& & Average&protein&length&in&three&domains&of&life&& & & & &&&&&&&&46& & Mechanisms&for&maintaining&protein&size&or&ORF&length&& & & &&&&&&&&47& & Properties&associated&with&protein&length&& & & & & &&&&&&&&52& Long&open&reading&frames&across&taxa&& & & & & &&&&&&&&55& Long&open&reading&frames&in&Porifera&& & & & & &&&&&&&&57& Large&orphan&proteins&in&Porifera&& & & & & & &&&&&&&&59& Conclusions&& & & & & & & & & &&&&&&&&62& References&& & & & & & & & & &&&&&&&&63& Tables&and&Figures&& & & & & & & & &&&&&&&&70& & iii CHAPTER&4.&GENERAL&CONCLUSIONS&& & & & & & &&&&&&&&94& & Summary&and&Recommendations&& & & & & & &&&&&&&&94& & & ACKNOWLEDGMENTS&& & & & & & & & &&&&&&&&96& & iv ABSTRACT! ! Sponges! (phylum! Porifera)! are! sessile,! filterbfeeding! animals! that! are! found! in! both!marine!and!freshwater!environments.!They!are!known!to!be!assoCiated!with!highly! diverse!and!stable!miCrobial!Communities.!The!first!Component!of!this!thesis!presents!a! study!on!the!diversity!of!the!miCrobial!Communities!assoCiated!with!freshwater!sponges! ColleCted!from!Lake!Baikal,!Baikalospongia+intermedia+profundalis!(BIP)!and!Lubomirskia+ baicalensis+ (LB).! Based! on! previous! studies,! miCrobial! Communities! assoCiated! with! freshwater!sponges!are!not!as!diverse!as!those!from!marine!sponges.!However,!one!of! the!potential!reasons!for!this!observation!is!the!limitation!posed!by!Conventional!Cloning! and!sequenCing!methods!used!in!previous!studies!on!freshwater!sponges.!To!gain!better! insight!into!the!diversity!of!the!miCrobiome!in!freshwater!sponges,!we!performed!highb throughput!sequenCing!of!PCRbamplified!16S!rRNA!genes!and!of!metagenome!without! prior! amplifiCation! in! two! sponge! speCies! BIP! and! LB.! Using! teChniques! that! involve! greater!sequenCing!Coverage,!we!Can!observe!that!freshwater!sponge!miCrobiomes!Can! be! as! diverse! as! marine! sponge! miCrobiomes.! The! miCrobial! Communities! of! the! freshwater!sponges!were!also!Compared!using!diversity!indiCes!and!it!was!observed!that! the! host! sponge! and! ColleCtion! depth! Can! both! influenCe! the! diversity! of! miCrobial! assoCiations!in!Lake!Baikal!sponges.! The!availability!and!ease!of!acCess!to!CostbeffeCtive!deep!sequenCing!teChniques! yielded!an!inCrease!in!the!number!of!Completely!sequenCed!genomes!of!organisms!in! three!domains!of!life!ArChaea,!Bacteria,!and!Eukarya.!This!inCrease!in!publiCly!available! v datasets!led!to!an!improved!understanding!on!the!general!properties!of!proteins!as!well! as!their!length!distributions.!The!seCond!Component!of!this!thesis!presents!a!review!of! studies!on!protein!length!distribution!and!the!observed!differenCes!in!average!protein! length!across!all!three!domains!of!life.!The!review!also!discusses!proposed!explanations! for!why!organisms!maintain!their!protein!size!and!presents!some!examples!of!proteins! that! defy! Constraints! on! protein! size! from! literature! and! from! genomes! of! sponges! Amphimedon+queenslandica,!Oscarella+carmela,!and!Baikalospongia+intermedia.! ! 1 CHAPTER(1( GENERAL(INTRODUCTION( ( Sponges((phylum(Porifera)(are(sessile,(filterEfeeding(animals(that(can(be(found(in( both(marine(and(freshwater(environments((Bergquist,(1978,(Penney,(1986).(They(harbor( numerous(microorganisms(that(can(range(from(archaeA(and(bacteriA(to(fungi,(diatoms,( dinoflagellates,( and( viruses( ( (Taylor( et( al.,( 2007).( The( first( component( of( this( thesis( presents( A( study( on( the( diversity( of( the( microbial( communities( associated( with( freshwater( sponges( collected( from( Lake( Baikal,( Baikalospongia+intermedia+profundalis( and(Lubomirskia+baicalensis.( The(availability(and(ease(of(access(to(costEeffective(deep(sequencing(techniques( yielded(an(increase(in(the(number(of(completely(sequenced(genomes(of(organisms(in(all( three( domains( of( life.( This( increase( in( publicly( available( datasets( led( to( An( improved( understanding(on(the(general(properties(of(proteins(as(well(as(their(length(distributions.( The( second( component( of( this( thesis( presents( A( review( of( studies( on( protein( length( distribution( and( the( observed( differences( in( average( protein( length( across( all( three( domains( of( life.( The( review( also( discusses( proposed( explanations( for( why( organisms( maintain( their( protein( size( and( presents( some( examples( of( proteins( that( defy( constraints(on(protein(size(from(literature(and(from(genomes(of(sponges(Amphimedon+ queenslandica,(Oscarella+carmela,(and(Baikalospongia+intermedia.( ( ( 2 Thesis!Organization! This! thesis! Contains! a! general! introduCtion! that! gives! an! overview! of! the! two! studies! (Chapter! 1),! a! study! on! the! diversity! of! miCrobial! Communities! in! Lake! Baikal! sponges! (Chapter! 2),! a! review! of! studies! on! protein! size! and! unusually! large! proteins!! (Chapter! 3),! and! a! general! ConClusion! that! summarizes! the! findings! and! reCommendations! (Chapter! 4).! Chapter! 2! presents! a! perspeCtive! on! the! diversity! of! miCrobiomes!in!freshwater!sponges!via!highbthroughput!sequenCing!of!metagenomes!of! Baikalospongia+ intermedia+ profundalis! and! Lubomirskia+ baicalensis.! Chapter! 3! is! a! review! of! studies! on! protein! length! in! ArChaea,! Bacteria,! and! Eukarya,! and! presents! some!examples!of!unusually!large!proteins!from!sponges!Amphimedon+queenslandica,! Oscarella+carmela,!and!Baikalospongia+intermedia.! ! Overview!1.!MiCrobial!Community!diversity!in!freshwater!sponges!from!Lake!Baikal! Sponges! form! Close! assoCiations! with! miCrobial! Communities! that! are! highly! diverse! and! stable! (HentsChel! et! al.,! 2002,! Taylor! et! al.,! 2007).! In! marine! sponges,! at! least! 28! bacterial! phyla! have! been! isolated! (HentsChel! et! al.,! 2012).! However,! information!about!miCroorganisms!assoCiated!with!sponges!in!freshwater!environments! is! limited.! Previous! studies! on! freshwater! sponges! Spongilla+ lacustris,! Lubomirskia+ baicalensis,! Ephydatia+ fluviatilis,! and! Baikalospongia+ intermedia! reported! 14! bacterial! phyla!(Gernert!et!al.,!2005,!Kaluzhnaya!et!al.,!2012,!Costa!et!al.,!2013,!Kaluzhnaya!and! ItskoviCh,! 2014),! of! whiCh! the! abundant! isolates! belong! to! groups! ACtinobacteria! and! Proteobacteria.!Based!on!these!studies,!it!is!presumed!that!freshwater!sponges!host!less! 3 diverse!miCrobial!Communities.!One!of!the!potential!reasons!for!this!observation!is!the! limitations!posed!by!Conventional!methods!(i.e.!denaturing!gradient!gel!eleCtrophoresis,! restriCtion! fragment! length! polymorphism,! 16S! rRNA! gene! amplifiCation,! cloning,! and! sequencing)!used!in!previous!studies!on!freshwater!sponges.!The!use!of!high!throughput! sequenCing!Can!reveal!a!better!perspeCtive!of!the!diversity!and!test!the!previous!notion! of!pauCity!of!assoCiated!miCroorganisms!in!freshwater!sponges.!! To!gain!better!insight!into!the!diversity!of!the!miCrobiome!in!freshwater!sponges,! we! performed!
Load more

Recommended publications
  • Proposal for a Revised Classification of the Demospongiae (Porifera) Christine Morrow1 and Paco Cárdenas2,3*
    Morrow and Cárdenas Frontiers in Zoology (2015) 12:7 DOI 10.1186/s12983-015-0099-8 DEBATE Open Access Proposal for a revised classification of the Demospongiae (Porifera) Christine Morrow1 and Paco Cárdenas2,3* Abstract Background: Demospongiae is the largest sponge class including 81% of all living sponges with nearly 7,000 species worldwide. Systema Porifera (2002) was the result of a large international collaboration to update the Demospongiae higher taxa classification, essentially based on morphological data. Since then, an increasing number of molecular phylogenetic studies have considerably shaken this taxonomic framework, with numerous polyphyletic groups revealed or confirmed and new clades discovered. And yet, despite a few taxonomical changes, the overall framework of the Systema Porifera classification still stands and is used as it is by the scientific community. This has led to a widening phylogeny/classification gap which creates biases and inconsistencies for the many end-users of this classification and ultimately impedes our understanding of today’s marine ecosystems and evolutionary processes. In an attempt to bridge this phylogeny/classification gap, we propose to officially revise the higher taxa Demospongiae classification. Discussion: We propose a revision of the Demospongiae higher taxa classification, essentially based on molecular data of the last ten years. We recommend the use of three subclasses: Verongimorpha, Keratosa and Heteroscleromorpha. We retain seven (Agelasida, Chondrosiida, Dendroceratida, Dictyoceratida, Haplosclerida, Poecilosclerida, Verongiida) of the 13 orders from Systema Porifera. We recommend the abandonment of five order names (Hadromerida, Halichondrida, Halisarcida, lithistids, Verticillitida) and resurrect or upgrade six order names (Axinellida, Merliida, Spongillida, Sphaerocladina, Suberitida, Tetractinellida). Finally, we create seven new orders (Bubarida, Desmacellida, Polymastiida, Scopalinida, Clionaida, Tethyida, Trachycladida).
    [Show full text]
  • Freshwater Adaptation at the Molecular Scale in the Unique Sponges of Lake Baikal
    bioRxiv preprint doi: https://doi.org/10.1101/416230; this version posted March 27, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Article: Discoveries Freshwater Adaptation at the Molecular Scale in the Unique Sponges of Lake Baikal Nathan J Kenny1 [email protected] Bruna Plese1,2 [email protected] Ana Riesgo1* [email protected] Valeria B. Itskovich3* [email protected] 1 Life Sciences Department, The Natural History Museum, Cromwell Road, London SW7 5BD, UK 2 Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000, Zagreb, Croatia 3 Limnological Institute, Siberian Branch of the Russian Academy of Science, Ulan-Batorskaya, 3, Irkutsk, 664033 Russia 1 bioRxiv preprint doi: https://doi.org/10.1101/416230; this version posted March 27, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract: The Lake Baikal ecosystem is unique. The largest, oldest and deepest lake in the world presents a variety of rare evolutionary opportunities and ecological niches to the species that inhabit it, and as a result the lake is a biodiversity hotspot. More than 80% of the animals found there are endemic, and they often exhibit unusual traits. The freshwater sponge Lubomirskia baicalensis and its relatives are good examples of these idiosyncratic organisms.
    [Show full text]
  • Formation of Spicules During the Long-Term Cultivation of Primmorphs from the Freshwater Baikal Sponge Lubomirskia Baikalensis L.I
    ry: C ist urr m en e t h R C Chernogor et al. Organic Chem Current Res 2011, S:2 e c s i e n a DOI: 10.4172/2161-0401.S2-001 a r c g r h O Organic Chemistry ISSN: 2161-0401 Current Research ResearchResearch Article Article OpenOpen Access Access Formation of Spicules During the Long-term Cultivation of Primmorphs from the Freshwater Baikal Sponge Lubomirskia baikalensis L.I. Chernogor1*, N.N. Denikina1, S.I. Belikov1 and A.V. Ereskovsky2,3 1Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya 3, Irkutsk 664033, Russia 2Department of Embryology, Faculty of Biology and Soils, Saint-Petersburg State University, Universitetskaja nab. 7/9, St. Petersburg 199034, Russia 3Centre d’Océanologie de Marseille, Station marine d’Endoume - CNRS UMR 6540-DIMAR, rue de la Batterie des Lions, 13007 Marseille, France Abstract Sponges (phylum Porifera) are phylogenetically ancient Metazoa that use silicon to form their skeletons. The process of biomineralization in sponges is one of the important problems being examined in the field of research focused on sponge biology. Primmorph cell culture is a convenient model for studying spiculogenesis. The aim of the present work was to produce a long-term primmorph culture from the freshwater Baikal sponge Lubomirskia baikalensis (class Demospongiae, order Haplosclerida and family Lubomirskiidae) in both natural Baikal water and artificial Baikal water to study the influence of silicate concentration on formation and growth of spicules in primmorphs. Silicate concentration plays an important role in formation and growth of spicules, as well as overabundance of silica leads to destruction of cell culture primmorphs.
    [Show full text]
  • Lake Baikal Bibliography, 1989- 1999
    UC San Diego Bibliography Title Lake Baikal Bibliography, 1989- 1999 Permalink https://escholarship.org/uc/item/7dc9945d Author Limnological Institute of RAS SB Publication Date 1999-12-31 eScholarship.org Powered by the California Digital Library University of California Lake Baikal Bibliography, 1989- 1999 This is a bibliography of 839 papers published in English in 1989- 1999 by members of Limnological Institute of RAS SB and by their partners within the framework of the Baikal International Center for Ecological Research. Some of the titles are accompanied by abstracts. Coverage is on different aspects of Lake Baikal. Adov F., Takhteev V., Ropstorf P. Mollusks of Baikal-Lena nature reserve (northern Baikal). // World Congress of Malacology: Abstracts; Washington, D.C.: Unitas Malacologica; 1998: 6. Afanasyeva E.L. Life cycle of Epischura baicalensis Sars (Copepoda, Calanoida) in Lake Baikal. // VI International Conference on Copepoda: Abstracts; July 29-August 3, 1996; Oldenburg/Bremerhaven, Germany. Konstanz; 1996: 33. Afanasyeva E.L. Life cycle of Epischura baicalensis Sars (Copepoda, Calanoida) in Lake Baikal. // J. Mar. Syst.; 1998; 15: 351-357. Epischura baicalensis Sars is a dominant pelagic species of Lake Baikal zooplankton. This is endemic to Lake Baikal and inhabits the entire water column. It produces two generations per year: the winter - spring and the summer. These copepods develop under different ecological conditions and vary in the duration of life stages, reproduction time, maturation of sex products and adult males and females lifespan. The total life period of the animals from each generation is one year. One female can produce 10 egg sacks every 10 - 20 days during its life time.
    [Show full text]
  • View Preprint
    A peer-reviewed version of this preprint was published in PeerJ on 26 May 2020. View the peer-reviewed version (peerj.com/articles/9080), which is the preferred citable publication unless you specifically need to cite this preprint. Chernogor L, Klimenko E, Khanaev I, Belikov S. 2020. Microbiome analysis of healthy and diseased sponges Lubomirskia baicalensis by using cell cultures of primmorphs. PeerJ 8:e9080 https://doi.org/10.7717/peerj.9080 Microbiome analysis of healthy and diseased sponges Lubomirskia baicalensis by using cell cultures of primmorphs Background. Freshwater sponges (Demosponges, Lubomirskiidae) are dominated in the littoral zone Lake Baikal in the biomass of benthic organisms and represent complex consortia of many species of eukaryotes and prokaryotes. A distinctive feature of sponges from Lake Baikal is their ability to live in symbiosis with various kinds of chlorophyll containing microalgae. Recently there have been massive diseases and the death of freshwater sponges. The etiology and ecology of these events remain unknown. Purpose. The purpose of the research was to use cell culture of primmorphs in vitro to study the microbiomes of healthy and diseased sponges to show the transmission of pathogenic agents from diseased sponges to cell cultures. Methods. The cell culture of primmorphs sponge Lubomirskia baicalensis was used to study microbiome communities in diseased and sick sponges in comparison with healthy sponge with subsequent sequencing of gene 16S rRNA and analysis of changes in microbiomes. Results. Results this study were show that use of cell culture of primmorphs in vitro is equivale of healthy sponge. Microbial community of healthy sponge and primmorphs was grouped separately from the community of diseased sponges and infected primmorphs, which confirms the suitability the cell culture of primmorphs, as a model sponge system.
    [Show full text]
  • Metagenomic Analysis of Viral Communities in Diseased Baikal Sponge Lubomirskia Baikalensis
    Limnology and Freshwater Biology 2019 (1): 155-162 DOI:10.31951/2658-3518-2019-A-1-155 Original Article Metagenomic analysis of viral communities in diseased Baikal sponge Lubomirskia baikalensis Butina T.V.1,*, Bukin Yu.S.1,2, Khanaev I.V.1, Kravtsova L.S.1, Maikova O.O.1, Tupikin A.E.3, Kabilov M.R.3, Belikov S.I.1 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, Irkutsk, 664033, Russia 2 Irkutsk National Research Technical University, Lermontov Str., 83, Irkutsk, 664074, Russia 3Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, Novosibirsk, 630090, Russia ABSTRACT. Sponges are an ecologically important component of marine and freshwater bodies. Sponge community includes a variety of microorganisms: fungi, algae, archaea, bacteria and viruses. Despite active research in the field of aquatic virology, biodiversity and the role of viruses in sponges are poorly studied. The relevance of research in this area is also related to the worldwide problem of sponge diseases. The aim of this study was to elucidate the genetic diversity of viruses in the associated community of diseased endemic Baikal sponge Lubomirskia baikalensis using metagenomic analysis. As a result, we have shown for the first time a high genetic and taxonomic diversity of DNA viruses in the Baikal sponge community. Identified sequences belonged to 16 viral families that infect a wide range of organisms. Moreover, our analysis indicated the differences in viral communities of visually healthy and diseased branches of the sponge. The approach used in this study is promising for further studies of viral communities in sponges, obtaining more complete information about the taxonomic and functional diversity of viruses in holobionts and entire Lake Baikal, and identifying the role of viruses in sponge diseases.
    [Show full text]
  • Helena Bilandžija
    Josip Juraj Strossmayer University of Osijek University of Dubrovnik Rudjer Boskovic Institute University Postgraduate Interdisciplinary Doctoral Study Molecular Biosciences Helena Bilandžija The molecular evolution of the cave animals PhD Dissertation Osijek, 2013 TEMELJNA DOKUMENTACIJSKA KARTICA Sveučilište Josipa Jurja Strossmayera u Osijeku Doktorski rad Sveučilište u Dubrovniku Institut Ruđer Bošković Sveučilišni poslijediplomski interdisciplinarni doktorski studij Molekularne bioznanosti Znanstveno područje: Prirodne znanosti Znanstveno polje: Biologija Molekularna evolucija špiljskih životinja Helena Bilandžija Rad je izrađen u: Laboratoriju za molekularnu genetiku Instituta Ruđer Bošković Mentor/i: dr.sc. Helena Ćetković Kratki sažetak doktorskog rada: Albinizam, najčešća prilagodba na život u podzemlju, evoluirao je promjenom na istom koraku sinteze melanina kod različitih špiljskih životinja što ukazuje na konvergentne i paralelne molekularne mehanizme adaptacija na život u špiljama. Posljedica istih adaptacija jest slična morfologija i molekularne su metode nužne za razumijevanje filogenetskih odnosa špiljskih životinja, a nude i uvid u zagonetku velike bioraznolikosti podzemlja Dinarida. Broj stranica: 147 Broj slika: 28 Broj tablica: 8 Broj literaturnih navoda: 167 Jezik izvornika: engleski Ključne riječi: podzemna fauna, albinizam, biosinteza melanina, filogenija, Dinaridi Datum obrane: Stručno povjerenstvo za obranu: 1. dr.sc. Goran Klobučar 2. dr.sc. Helena Ćetković 3. dr.sc. Vera Cesar 4. 5. 6. (zamjena) dr.sc. Andreja
    [Show full text]
  • BIBLIOGRAPHY ABRIKOSOV, G. G., 1924. to the Knowledge of The
    BIBLIOGRAPHY ABRIKOSOV, G. G., 1924. To the knowledge of the bryozoan fauna of Lake BaikaI. Russ. Hydrob. ]., Saratov, 11-12: 260-265*. ABRIKOSOV, G. G., 1927. On the freshwater bryozoans of the USSR.Doklady Aead. Sei. USSR, 19: 307-372*. ABRIKOSOV, G. G., 1959. On the generie divisions and geographie al distribution of the Gymnolemata bryozoans of eontinental waters. Doklady Aead. Sei. USSR, 126,6: 7378-7380*. AFANASYEV, A. N., 1960. The water budget of Lake Baikal. Trudy Baik. limnol. St. Aead. Sei. USSR, XVIII: 755-247*. AKATOVA, N. A., 1949. The zooplankton of the River Kolyam and its drainage. Trans. Leningr. Univ., 126, biol. ser., 21: 347*. AKHMEROV, A. K. & DOMBROVSKAYA-AKHMEROVA, O. S., 1941. New Aeanthoeephala from the fishes of the River Amur. Doklady Aead. Sei. USSR, XXXI, 5: 517-520*. ALPATOV, V. V., 1923. A water-bug new to Baikai and a eomparison of the Asellus speeies of Baikai with those of Europe and Ameriea. Russ. hydrobiol. ]., Saratov, 3-4: 64-66*. ANDRUSOV, N. 1., 1902. On two new gastropod speeies from the Apsheron stage. Trudy SPB Nat. Soe., geol. and mineral. dept., XXXI, 5: 55-75*. ANNANDLE, N., 1911 (1912). Systematie notes on the Stenostomus Polyzoa Oi freshwater. Rec. Indian Mus. Caleufta, IV, 4. ANNANDLE, N., 1913. Notes on some sponges from Lake Baikai in the eolleetion of the Imp. Aeademy of Science. St. Petersburg. Yearb. Zool. Mus. Aead. Sei., XVIII: 96-707. ANNENKOVA, N. P., 1930. Fresh and braekish water Polyehaeta of the USSR. In Determinative tables of freshwater organisms of the USSR. Freshwater fauna, 2, Leningrad.
    [Show full text]
  • V.V. Takhteev. Trends in the Evolution of Baikal Amphipods And
    Trends in the Evolution of Baikal Amphipods and Evolutionary Parallels with some Marine Malacostracan Faunas V.V. TAKHTEEV I. Summary 197 II. Introduction.......... 198 III. Basic Tendencies in the Evolution of Baikalian Amphipods 199 A. Bathymetric Segregation.......... 199 B. Differentiation by Season of Reproduction 201 C. Segregation in Terms of Substrate Layer........................................... 203 D. Differentiation by Substrate 204 E. Trophic Differentiation 205 F. Transition to Marsupial Parasitism.................................................... 205 G. Appearance of Giant Forms 206 H. Appearance of Dwarf Forms (Hypomorphosis) 207 I. Occupation of the Shallow Gulf Habitats of the Lake...................... 207 J. Geographical Differentiation.............................................................. 209 IV. Parallel (Nomogenetic) Development of the Baikalian and Marine Malocostracan Faunas............................................................................... 211 Acknowledgements..................................................................................... 215 References................................................................................................ .. 215 I. SUMMARY The taxonomically rich amphipod fauna of Lake Baikal (at present 257 described species and 74 subspecies) shows great ecological diversity and a variety of evolutionary directions. The main trends in the intralacustrine evolution of this group are: habitat partitioning by depth, substrate type or layer; trophic differentiation;
    [Show full text]
  • A New Species of Baikal Endemic Sponges (Porifera, Demospongiae
    A peer-reviewed open-access journal ZooKeys 906: 113–130 (2020) A new species of Baikal endemic sponges 113 doi: 10.3897/zookeys.906.39534 RESEARCH ARTICLE http://zookeys.pensoft.net Launched to accelerate biodiversity research A new species of Baikal endemic sponges (Porifera, Demospongiae, Spongillida, Lubomirskiidae) Natalia A. Bukshuk1, Olga O. Maikova1 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str. 3, 664 033 Irkutsk, Russia Corresponding author: Olga O. Maikova ([email protected]) Academic editor: Roberto Pronzato | Received 29 August 2019 | Accepted 16 December 2019 | Published 22 January 2020 http://zoobank.org/2EC18723-4B0F-43BF-B27A-AA5A9D752E27 Citation: Bukshuk NA, Maikova OO (2020) A new species of Baikal endemic sponges (Porifera, Demospongiae, Spongillida, Lubomirskiidae). ZooKeys 906: 113–130. https://doi.org/10.3897/zookeys.906.39534 Abstract This paper reports on a new species of the Baikal endemic sponge (fam. Lubomirskiidae) Swartschewskia khanaevi sp. nov. The description of this species is based on morphological and molecular data (ITS and mitochondrial IGRs). Morphologically, S. khanaevi sp. nov. differs fromS. papyracea by loose tracts arranged in an irregular network as well as the presence on strongyles of compound spines looking like tubercles densely ornamented with simple spines. Moreover, specimens of S. khanaevi sp. nov. show a peculiar structure of the aquiferous system at the body surface that may be an adaptive trait for environ- mental conditions. Phylogenetic analysis has revealed that S. khanaevi sp. nov. forms a well-supported (0.99) monophyletic clade with S. papyracea and is allocated as its sister taxa. Keywords ITS, mitochondrial IGRs, morphological analysis, Swartschewskia Introduction Baikal is the most ancient and deepest lake on the Earth with the huge water volume.
    [Show full text]
  • Sponges Lubomirskiidae As Bioindicators of the State of Lake Baikal
    Limnology and Freshwater Biology 2019 (1): 172-176 DOI:10.31951/2658-3518-2019-A-1-172 Original Article Sponges Lubomirskiidae as bioindicators of the state of Lake Baikal Itskovich V.B.1,*, Shigarova A.M.2, Glyzina O.Y.1, Kaluzhnaya O.V.1, Borovskii G.B.2, Selvin J.3 1 Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya 3, 664 033 Irkutsk, Russia. 2 Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy оf Sciences, Lermontova 132, P.O. Box 317, 664033, Irkutsk, Russia 3 Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India ABSTRACT. Sponges are ideal bioindicators of the environmental state due to their simple body structure, filter-feeding lifestyle and widespread abundance.For marine sponges, stress response studies are conducted in the field of microbial composition, gene expression and transcriptome techniques. For freshwater sponges expression of the stress protein HSP70 was studied. In the Baikal sponges, the content of HSP70 is an indicator of the stress response to temperature increase. Transcriptomic studies of the endemic Baikal sponges are in progress. Keywords: Porifera, bioindicators, Lake Baikal, Lubomirskiidae, HSP70, hyperthermia 1. Introduction on these parameters are limited, although sponges have shown to be very sensitive to changes in surrounding Sponges (Porifera) are the earliest multicellular conditions (López-Legentil et al., 2008; Pantile and animals and important component of marine and Webster, 2011). freshwater ecosystems (Diaz and Rützler, 2001; Bell, For marine sponges, stress response studies 2008; Van Soest et al., 2012; Webster et al., 2013).
    [Show full text]
  • Analysis of Cross-Species Usability of Microsatellite Markers for Baikal Endemic Sponges
    Proceedings Analysis of Cross-Species Usability of Microsatellite Markers for Baikal Endemic Sponges Alena Yakhnenko 1,2,* and Valeria Itskovich 1 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya, Irkutsk 664033, Russia; [email protected] 2 International Intergovernmental Organization Joint Institute for Nuclear Research, 6 Joliot-Curie St, Dubna, 141980, Russia * Correspondence: [email protected]; Tel.: +7-999-420-45-08 Abstract: In the last decade, events of mass disease and mortality of sponges have been observed on Lake Baikal, which indicates an ecological crisis on the Lake. Based on the crucial role of sponges as filter feeders and bioindicators, their comprehensive study in this situation is of great interest. Despite the presence of genomic and transcriptome data for several species of endemic Baikal sponges, their population structure has never been studied before. The analysis of the population structure of both marine and freshwater sponges is successfully carried out using microsatellite markers. For freshwater sponges, the only species for which microsatellite markers have been pub- lished is Ephydatia fluviatilis, a close relative of the Baikal endemic sponges. Microsatellite markers show a high percentage of interspecies cross-specificity among invertebrates. According to this, we attempted here to assess the suitability of these microsatellite markers for population genetic studies of endemic Baikal sponge Lubomirskia baikalensis based on genomic data. The presence of mi- crosatellite sequence markers homologous to the flanking regions in the L.baikalensis genome was shown for 63.6% of markers, 71.4% of which contained microsatellite sequences. However, all of these markers require the development of species-specific primer pairs.
    [Show full text]