DOCSLIB.ORG

Isoleucilactucin Ameliorates Coal Fly Ash-Induced Inflammation Through

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Isoleucilactucin Ameliorates Coal Fly Ash-Induced Inflammation Through International Journal of Molecular Sciences Article Isoleucilactucin Ameliorates Coal Fly Ash-Induced Inflammation through the NF-κB and MAPK Pathways in MH-S Cells H. M. Arif Ullah 1 , Tae-Hyung Kwon 2,*, SeonJu Park 3 , Sung Dae Kim 1 and Man Hee Rhee 1,* 1 Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea; [email protected] (H.M.A.U.); [email protected] (S.D.K.) 2 Department of Research and Development, Chuncheon Bio-Industry Foundation (CBF), Chuncheon 24232, Korea 3 Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea; [email protected] * Correspondence: [email protected] (T.-H.K.); [email protected] (M.H.R.); Tel.: +82-33-258-6993 (T.-H.K.); +82-53-950-5967 (M.H.R.) Abstract: We investigated whether isoleucilactucin, an active constituent of Ixeridium dentatum, reduces inflammation caused by coal fly ash (CFA) in alveolar macrophages (MH-S). The anti- inflammatory effects of isoleucilactucin were assessed by measuring the concentration of nitric oxide (NO) and the expression of pro-inflammatory mediators in MH-S cells exposed to CFA-induced inflammation. We found that isoleucilactucin reduced CFA-induced NO generation dose-dependently in MH-S cells. Moreover, isoleucilactucin suppressed CFA-activated proinflammatory mediators, including cyclooxygenase-2 (COX2) and inducible NO synthase (iNOS), and the proinflammatory cytokines such as interleukin-(IL)-1β, IL-6, and tumor necrosis factor (TNF-α). The inhibiting Citation: Ullah, H.M.A.; Kwon, properties of isoleucilactucin on the nuclear translocation of phosphorylated nuclear factor-kappa B T.-H.; Park, S.; Kim, S.D.; Rhee, M.H. (p-NF-κB) were observed. The effects of isoleucilactucin on the NF-κB and mitogen-activated protein Isoleucilactucin Ameliorates Coal Fly kinase (MAPK) pathways were also measured in CFA-stimulated MH-S cells. These results indicate Ash-Induced Inflammation through that isoleucilactucin suppressed CFA-stimulated inflammation in MH-S cells by inhibiting the NF-κB the NF-κB and MAPK Pathways in and MAPK pathways, which suggest it might exert anti-inflammatory properties in the lung. MH-S Cells. Int. J. Mol. Sci. 2021, 22, 9506. https://doi.org/10.3390/ Keywords: lung macrophages; isoleucilactucin; anti-inflammatory properties; NF-κB pathway; ijms22179506 AMPK pathway Academic Editor: Stefania Marzocco Received: 13 August 2021 1. Introduction Accepted: 29 August 2021 Published: 1 September 2021 Inflammation is a critical aspect of the immune response against cell damage and pathogens, which also provides a defense mechanism to remove harmful stimuli [1–3]. Publisher’s Note: MDPI stays neutral Increased movement of plasma and leukocytes from the blood into wounded regions with regard to jurisdictional claims in causes acute inflammation, which is an initial response of the organism to damaging published maps and institutional affil- stimuli. When it becomes chronic, inflammation causes a gradual shift in the cell types iations. present at the site of inflammation and can result in a destruction or regeneration of tissues, depending on the context [4]. Dysregulated inflammation is associated with various chronic diseases [2,5]. Macrophages play a crucial role in inflammatory processes, primarily by producing proinflammatory mediators that include nitric oxide (NO), inducible NO Copyright: © 2021 by the authors. synthase (iNOS), cyclooxygenase-2 (COX-2), as well as the cytokines interleukin (IL)-1β, Licensee MDPI, Basel, Switzerland. IL-6, and tumor necrosis factor-α (TNF-α)[6,7]. Therefore, inhibiting proinflammatory This article is an open access article macrophage transformation is considered a critical strategy in the treatment of numerous distributed under the terms and inflammatory disorders. conditions of the Creative Commons In the last few decades, environmental contaminants were shown to cause immuno- Attribution (CC BY) license (https:// logic dysfunction in chronic respiratory inflammatory disorders: asthma, chronic respira- creativecommons.org/licenses/by/ tory diseases, chronic obstructive pulmonary disease (COPD), and various types of lung 4.0/). Int. J. Mol. Sci. 2021, 22, 9506. https://doi.org/10.3390/ijms22179506 https://www.mdpi.com/journal/ijms Int. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW 2 of 13 In the last few decades, environmental contaminants were shown to cause immuno- logic dysfunction in chronic respiratory inflammatory disorders: asthma, chronic respira- Int. J. Mol. Sci. 2021, 22, 9506 2 of 12 tory diseases, chronic obstructive pulmonary disease (COPD), and various types of lung cancer [1,6,8–10]. COPD is the world’s third leading cause of death, with 3 million indi- viduals dying from the disease in 2016 [11]. In modern industrial countries, particulate mattercancer (PM) [1,6,8 represents–10]. COPD the is most the world’s dangerous third ai leadingr contaminant cause of [12]. death, The with main 3 millionsources individ- of PM includeuals dying outdoor from pollutants the disease from in 2016 gasoline [11]. In fuel modern burning industrial in automobiles countries, and particulate industries, matter as well(PM) as represents indoor pollutants the most from dangerous cigarette air smok contaminante and culinary [12]. The exhausts main sources [6,13]. ofAccording PM include to previousoutdoor research, pollutants exposure from gasoline to PM has fuel been burning linked in automobilesto worseningand of asthma industries, symptoms, as well as as wellindoor as increased pollutants hospitalization from cigarette smoke and mortality and culinary in individuals exhausts [6 with,13]. AccordingCOPD [14,15]. to previous Expo- sureresearch, to PM exposure could induce to PM alveolar has been macropha linked toges worsening to produce of asthmaexcessive symptoms, amounts asof wellproin- as flammatoryincreased hospitalization mediators, such and as mortalityiNOS, COX2, in individuals TNF-α, IL-1 withβ, and COPD IL-6, [14which,15]. determine Exposure toa widePM couldrange induceof pathological alveolar macrophagesand clinical symptoms to produce [9,16]. excessive amounts of proinflammatory α β mediators,The pharmaceutical such as iNOS, industry’s COX2, TNF- interest, IL-1 in, natural and IL-6, drugs which has determine grown, thus a wide including range of naturalpathological resources and clinicalin drug symptomsdevelopment [9,16 [3,17,]. 18]; indeed, over 100 medications derived The pharmaceutical industry’s interest in natural drugs has grown, thus including from natural products are now being tested in clinical trials [3,19]. Isoleucilactucin, one of natural resources in drug development [3,17,18]; indeed, over 100 medications derived the major active constituents of Ixeridium dentatum, has been reported to have amylase from natural products are now being tested in clinical trials [3,19]. Isoleucilactucin, one secretion activity after treatment with high glucose in human salivary gland cells [20]. of the major active constituents of Ixeridium dentatum, has been reported to have amylase Coal fly ash (CFA) is made up of a variety of liquid and solid PM, including asbestos, coal, secretion activity after treatment with high glucose in human salivary gland cells [20]. and combustion particles of various sizes and origins [1,6,21]. However, the effect of iso- Coal fly ash (CFA) is made up of a variety of liquid and solid PM, including asbestos, leucilactucin in the CFA-stimulated inflammatory cascade on alveolar macrophages (MH- coal, and combustion particles of various sizes and origins [1,6,21]. However, the effect S) remains elusive. The goal of this study is to determine whether isoleucilactucin has anti- of isoleucilactucin in the CFA-stimulated inflammatory cascade on alveolar macrophages inflammatory activities upon CFA-induced inflammatory stimulation of MH-S cells. (MH-S) remains elusive. The goal of this study is to determine whether isoleucilactucin has anti-inflammatory activities upon CFA-induced inflammatory stimulation of MH-S cells. 2. Results 2.1.2. ResultsIsoleucilactucin Protected against CFA-Induced Nitric Oxide Production and Cell Death in Alveolar2.1. Isoleucilactucin Macrophage Protected(MH-S) Cells against CFA-Induced Nitric Oxide Production and Cell Death in Alveolar Macrophage (MH-S) Cells Nitric oxide (NO) is a vital regulator in the inflammatory process, while excess NO contributesNitric oxideto the (NO)development is a vital regulatorof numerous in the inflammatory inflammatory disorders process, while[22–24]. excess In our NO study,contributes the NO to levels the development in murine MH-S of numerous cells in inflammatoryresponse to CFA disorders stimulation [22–24 were]. In ourassessed study, usingthe NO the levels Griess in reaction. murine MH-S Isoleucilactucin cells in response decreased to CFA NO stimulation induction werepotently assessed and dose-de- using the pendentlyGriess reaction. (Figure Isoleucilactucin 1A). decreased NO induction potently and dose-dependently (Figure1A). FigureFigure 1. 1. EffectEffect of of isoleucilactucin isoleucilactucin on on coal coal fly fly ash ash (CFA)-induced (CFA)-induced nitric nitric oxide oxide (NO) (NO) generation generation and and viabilityviability in in MH-S MH-S cells. cells. (A (A) )Cells Cells were were categorized categorized into into six six groups: groups: basal basal (control), (control), CFA CFA (2.5 (2.5 μµg/mL),g/mL), andand four four concentrations concentrations of of CFA CFA with with isoleucilactucin isoleucilactucin (12.5, (12.5, 25, 25, 50, 50, and and 100 100 μµM).M).
Load more

Recommended publications
  • Potential Impact of an Exotic Plant Invasion on Both Plant and Arthropod Communities in a Semi-Natural Grassland on Sugadaira Montane in Japan
    Journal of Developments in Sustainable Agriculture 12: 52-64 (2017) Potential Impact of an Exotic Plant Invasion on Both Plant and Arthropod Communities in a Semi-natural Grassland on Sugadaira Montane in Japan Yukie Sato1*, Yuta Mashimo1,2, Ryo O. Suzuki1, Akira S. Hirao1, Etsuro Takagi1,3, Ryuji Kanai1, Daisuke Masaki1, Miyuki Sato1 and Ryuichiro Machida1 1 Sugadaira Montane Research Center, University of Tsukuba, Sugadaira Kogen 1278-294, Ueda, Nagano 386-2204, Japan 2 Graduate School of Symbiotic Systems Science and Technology, Fukushima University, Kanayagawa 1, Fukushima, Fukushima 960-1296, Japan 3 Department of Tourism Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan Plant and arthropod communities interact closely with one another, therefore, invasive plants can alter not only plant communities, but may also have direct and indirect effects on arthropod communities. Here, we focus on the exotic giant ragweed, which is a serious invasive weed in Japan. Recently, the exotic plant invaded and has dominated part of a semi-natural grassland in Sugadaira Montane Research Center (Nagano Prefecture, Japan). We attempted to evaluate the potential impact of the invasive plant on both plant and arthropod communities by comparing the com- munity composition, abundance, species richness and diversity indices of plants and arthropods between areas where the exotic giant ragweed had and had not invaded, referred to as the invaded and reference areas respectively. We found significant differences in plant and arthropod community compositions between the areas. Plant species richness was lower in the invaded area as predicted. However, the abundance of arthropods including herbivores was higher in the invaded area compared to the reference area in contrast to the expectation that plant invasions reduce arthropod abundance and diversity.
    [Show full text]
  • Chapter 4 Phytogeography of Northeast Asia
    Chapter 4 Phytogeography of Northeast Asia Hong QIAN 1, Pavel KRESTOV 2, Pei-Yun FU 3, Qing-Li WANG 3, Jong-Suk SONG 4 and Christine CHOURMOUZIS 5 1 Research and Collections Center, Illinois State Museum, 1011 East Ash Street, Springfield, IL 62703, USA, e-mail: [email protected]; 2 Institute of Biology and Soil Science, Russian Academy of Sciences, Vladivostok, 690022, Russia, e-mail: [email protected]; 3 Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, Shenyang 110015, China; 4 Department of Biological Science, College of Natural Sciences, Andong National University, Andong 760-749, Korea, e-mail: [email protected]; 5 Department of Forest Sciences, University of British Columbia, 3041-2424 mail Mall, Vancouver, B.C., V6T 1Z4, Canada, e-mail: [email protected] Abstract: Northeast Asia as defined in this study includes the Russian Far East, Northeast China, the northern part of the Korean Peninsula, and Hokkaido Island (Japan). We determined the species richness of Northeast Asia at various spatial scales, analyzed the floristic relationships among geographic regions within Northeast Asia, and compared the flora of Northeast Asia with surrounding floras. The flora of Northeast Asia consists of 971 genera and 4953 species of native vascular plants. Based on their worldwide distributions, the 971 gen- era were grouped into fourteen phytogeographic elements. Over 900 species of vascular plants are endemic to Northeast Asia. Northeast Asia shares 39% of its species with eastern Siberia-Mongolia, 24% with Europe, 16.2% with western North America, and 12.4% with eastern North America.
    [Show full text]
  • Phylogenetic Relationships Within Lactuca L. (Asteraceae), Including African Species, Based on Chloroplast DNA Sequence Comparisons
    Genet Resour Crop Evol DOI 10.1007/s10722-015-0332-5 RESEARCH ARTICLE Phylogenetic relationships within Lactuca L. (Asteraceae), including African species, based on chloroplast DNA sequence comparisons Zhen Wei . Shi-Xin Zhu . R. G. Van den Berg . Freek T. Bakker . M. Eric Schranz Received: 25 April 2015 / Accepted: 29 September 2015 Ó The Author(s) 2015. This article is published with open access at Springerlink.com Abstract Lettuce (Lactuca sativa L.) belongs to the maximum likelihood and Bayesian inference analyses. genus Lactuca L. and is an important vegetable world- Biogeographical, chromosomal and morphological wide. Over the past decades, there have been many character states were reconstructed over the Bayesian controversies about the phylogeny of Lactuca species tree topology. We conclude that Lactuca contains two due to their complex and diverse morphological distinct phylogenetic clades—the crop clade and the characters and insufficient molecular sampling. In this Pterocypsela clade. Other North American, Asian and study we provide the most extensive molecular widespread species either form smaller clades or mix phylogenetic reconstruction of Lactuca, including with the Melanoseris species. The newly sampled African wild species, using two chloroplast genes African endemic species probably should be treated as (ndhF and trnL-F). The sampling covers nearly 40 % a new genus. of the total endemic African Lactuca species and 34 % of the total Lactuca species. DNA sequences from all Keywords African Lactuca Á Lactuca phylogeny Á the subfamilies of Asteraceae in Genebank and those Lettuce Á ndhF Á Phylogenetic relationships Á trnL-F generated from Lactuca herbarium samples were used to establish the affiliation of Lactuca within Astera- caeae.
    [Show full text]
  • Genetic Diversity and Evolution in Lactuca L. (Asteraceae)
    Genetic diversity and evolution in Lactuca L. (Asteraceae) from phylogeny to molecular breeding Zhen Wei Thesis committee Promotor Prof. Dr M.E. Schranz Professor of Biosystematics Wageningen University Other members Prof. Dr P.C. Struik, Wageningen University Dr N. Kilian, Free University of Berlin, Germany Dr R. van Treuren, Wageningen University Dr M.J.W. Jeuken, Wageningen University This research was conducted under the auspices of the Graduate School of Experimental Plant Sciences. Genetic diversity and evolution in Lactuca L. (Asteraceae) from phylogeny to molecular breeding Zhen Wei Thesis submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr A.P.J. Mol, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Monday 25 January 2016 at 1.30 p.m. in the Aula. Zhen Wei Genetic diversity and evolution in Lactuca L. (Asteraceae) - from phylogeny to molecular breeding, 210 pages. PhD thesis, Wageningen University, Wageningen, NL (2016) With references, with summary in Dutch and English ISBN 978-94-6257-614-8 Contents Chapter 1 General introduction 7 Chapter 2 Phylogenetic relationships within Lactuca L. (Asteraceae), including African species, based on chloroplast DNA sequence comparisons* 31 Chapter 3 Phylogenetic analysis of Lactuca L. and closely related genera (Asteraceae), using complete chloroplast genomes and nuclear rDNA sequences 99 Chapter 4 A mixed model QTL analysis for salt tolerance in
    [Show full text]
  • 5. Tribe CICHORIEAE 菊苣族 Ju Ju Zu Shi Zhu (石铸 Shih Chu), Ge Xuejun (葛学军); Norbert Kilian, Jan Kirschner, Jan Štěpánek, Alexander P
    Published online on 25 October 2011. Shi, Z., Ge, X. J., Kilian, N., Kirschner, J., Štěpánek, J., Sukhorukov, A. P., Mavrodiev, E. V. & Gottschlich, G. 2011. Cichorieae. Pp. 195–353 in: Wu, Z. Y., Raven, P. H. & Hong, D. Y., eds., Flora of China Volume 20–21 (Asteraceae). Science Press (Beijing) & Missouri Botanical Garden Press (St. Louis). 5. Tribe CICHORIEAE 菊苣族 ju ju zu Shi Zhu (石铸 Shih Chu), Ge Xuejun (葛学军); Norbert Kilian, Jan Kirschner, Jan Štěpánek, Alexander P. Sukhorukov, Evgeny V. Mavrodiev, Günter Gottschlich Annual to perennial, acaulescent, scapose, or caulescent herbs, more rarely subshrubs, exceptionally scandent vines, latex present. Leaves alternate, frequently rosulate. Capitulum solitary or capitula loosely to more densely aggregated, sometimes forming a secondary capitulum, ligulate, homogamous, with 3–5 to ca. 300 but mostly with a few dozen bisexual florets. Receptacle naked, or more rarely with scales or bristles. Involucre cylindric to campanulate, ± differentiated into a few imbricate outer series of phyllaries and a longer inner series, rarely uniseriate. Florets with 5-toothed ligule, pale yellow to deep orange-yellow, or of some shade of blue, including whitish or purple, rarely white; anthers basally calcarate and caudate, apical appendage elongate, smooth, filaments smooth; style slender, with long, slender branches, sweeping hairs on shaft and branches; pollen echinolophate or echinate. Achene cylindric, or fusiform to slenderly obconoidal, usually ribbed, sometimes compressed or flattened, apically truncate, attenuate, cuspi- date, or beaked, often sculptured, mostly glabrous, sometimes papillose or hairy, rarely villous, sometimes heteromorphic; pappus of scabrid [to barbellate] or plumose bristles, rarely of scales or absent.
    [Show full text]
  • Biosystematic Studies on the Genus Ixeris − （Compositae Lactuceae）1． Fruit Wali Anatomy and Its Taxonomic Implications
    The Japanese SocietySooiety for Plant SystematicsSystematios − − ISSN OOO1 6799 Acta Phytotax． Geobot．41 ： 43 60 （1990） Biosystematic Studies on the Genus Ixeris − （Compositae Lactuceae）1． Fruit WalI Anatomy and Its Taxonomic Implications − JaeHong PAKI and Shoichi KAwANo2 ・ 一 朴 宰 弘 河 野 昭 ： ニ ガ ナ属 （キ ク科 ・タ ン ポ ポ 連 ） の 分 類 学 的 研 究 1． 果皮 の 解 剖 学 的 特 徴 と そ の 分 類 学 的 意 義 Abstract The mature fruit wall structures were invcstigated fer l80f about 20 傭 癇 spec 三es − （Compos 童tae Lactucea ¢ 〉． Fourteen species had 10 costae ， nearly the same size in transverse sections and fbur species had − lO− − ， 5 70r 13 costae ， Fruit waU structures were divided intotwo ． maimypes ： wi nged type subdiv 蓋ded into Type I and Type ILA B G and ribbed type Type （ ， ， ）， （ III ．Type I has thick 量ntercosta L repens Type II−A B and G have weU −developed costae ） （ ’）； ， 孟 ‘ePhala ， 1． debilis五 5 ‘oJo η γα L longirestrata 孟 ‘海 η 8 π 廊 and L tamagatvaensi ． The ribbed （ Poly ， 静 ， ， の type （Type III）has the least conspicuous costae （1． dentata and 工Oother species of sect ． Ixeridium）． In the winged type ， costae are f（）rmed by cell divisiens in the i nitial stage of costa1 tissue develop− ment was ， but its development less conspicuous in the intercostal tissuc of ∬． rep 砌 50f Type L In the ribbed type cQstae are of rather ， fbrmed by the disintcgration intercostaltissue ， than by − − cell divisions in costal tissue throughout pre and post fとrtil 重zation stages ． Based on the different fruit−wall types and ontogeneticprocesses as well ， as on karyological data， we suggest
    [Show full text]
  • The Pennsylvania State University
    The Pennsylvania State University The Graduate School HOME GARDENS AS AGROBIODIVERSITY SITES AMID AGRARIAN TRANSFORMATIONS IN JEJU, KOREA (1960–2016) A Dissertation in Geography by Yooinn Hong 2021 Yooinn Hong Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August 2021 The dissertation of Yooinn Hong was reviewed and approved by the following: Karl S. Zimmerer Professor of Geography Dissertation Advisor Chair of Committee Brian King Professor of Geography Erica A. H. Smithwick Professor of Geography Leland Glenna Professor of Rural Sociology Cynthia Brewer Professor of Geography Head of the Department of Geography ii ABSTRACT Geographic research has focused on identifying spaces of agrobiodiversity amid rural changes that may threaten continued cultivation and the future of farmer-based evolution. This dissertation investigates home gardens as important agrobiodiversity sites in rural Jeju, South Korea, where land and livelihoods have been fundamentally transformed over the past few decades as a result of widespread adoption of commercial cropping and livelihood diversification associated with agricultural modernization and tourism development. The dissertation draws upon and contributes to geographic literature in three broad areas: the environment–society geographical investigations of home gardens as agrobiodiversity sites, the political ecology investigation of the state’s role in agrarian development and modernization, and geographies of agrarian transformations, especially agricultural commercialization and livelihood diversification. The findings demonstrate how local people have re-configured home garden agrobiodiversity in response to agrarian changes. The research also tests several environment–society hypotheses currently under debate regarding factors influencing home garden cultivation practice and agrobiodiversity. Three broad sets of questions guide the dissertation’s research.
    [Show full text]
  • The Tribe Cichorieae In
    Chapter24 Cichorieae Norbert Kilian, Birgit Gemeinholzer and Hans Walter Lack INTRODUCTION general lines seem suffi ciently clear so far, our knowledge is still insuffi cient regarding a good number of questions at Cichorieae (also known as Lactuceae Cass. (1819) but the generic rank as well as at the evolution of the tribe. name Cichorieae Lam. & DC. (1806) has priority; Reveal 1997) are the fi rst recognized and perhaps taxonomically best studied tribe of Compositae. Their predominantly HISTORICAL OVERVIEW Holarctic distribution made the members comparatively early known to science, and the uniform character com- Tournefort (1694) was the fi rst to recognize and describe bination of milky latex and homogamous capitula with Cichorieae as a taxonomic entity, forming the thirteenth 5-dentate, ligulate fl owers, makes the members easy to class of the plant kingdom and, remarkably, did not in- identify. Consequently, from the time of initial descrip- clude a single plant now considered outside the tribe. tion (Tournefort 1694) until today, there has been no dis- This refl ects the convenient recognition of the tribe on agreement about the overall circumscription of the tribe. the basis of its homogamous ligulate fl owers and latex. He Nevertheless, the tribe in this traditional circumscription called the fl ower “fl os semifl osculosus”, paid particular at- is paraphyletic as most recent molecular phylogenies have tention to the pappus and as a consequence distinguished revealed. Its circumscription therefore is, for the fi rst two groups, the fi rst to comprise plants with a pappus, the time, changed in the present treatment. second those without.
    [Show full text]
  • Biosystematic Studies on the Genus Ixeris (Compositae-Lactuceae) II
    _??_1990 by Cytologia, Tokyo Cytologia 55: 553-570 , 1990 Biosystematic Studies on the Genus Ixeris (Compositae-Lactuceae) II. Karyological analyses Jae-Hong Pak1 and Shoichi Kawano2 1 Department of Botany, Faculty of Science , Kyoto University, Kyoto 606, Japan 2Department of Botany , Faculty of Science, Kyoto University, Kyoto 606, and Institute of Genetic Ecology, Tohoku University, Sendai 980, Japan Accepted April 20, 1990 Earlier karyological studies of the genus Ixeris and related groups are represented by those of Ishikawa (1921) and Babcock et al. (1937) (also cf. Takemoto 1954, 1956, 1962, 1970, Nisioka 1956, 1960, 1963, Fujishima 1984). Ishikawa (1921) studied the chromosome numbers of 20 Lactuca species from Japan, and showed that Lactuca (sensu Bentham and Hooker 1873, cum Ixeris) comprises some heterogeneous groups, with four different basic chromosome numbers, x=5, 7, 8 and 9. He further pointed out that the basic chromosome numbers and size are important criteria for the delimitation of the genera and assessment of the species relationships in Lactuca s. lat., demonstrating that those with x=5, 7, 8 and 9 base numbers are in good agreement with Nakai's generic concept (1920): Crepidiastrum (x=5), Paraixeris (x=5), Ixeris (x=7, 8), and Lactuca (x=9). However, later on Babcock et al. (1937), on the basis of an ex tensive karyological study of Crepidinae, claimed that the genus Ixeris (sensu Stebbins 1937) consists of the species with three different basic chromosome numbers, x=5, 7 and 8, which contradicts Ishikawa's viewpoint (1921). The genus Ixeris (Compositae-Lactuceae) is at present known to comprise about 20 species (Nakai 1920, Kitamura 1956, 1981) or 50 species, including some species of Crepidiastrum, Paraixeris and even Lactuca (Stebbins 1937, Tomb 1977).
    [Show full text]
  • Traditional Use and Management of Ntfps in Kangchenjunga Landscape: Implications for Conservation and Livelihoods Yadav Uprety1*, Ram C
    Uprety et al. Journal of Ethnobiology and Ethnomedicine (2016) 12:19 DOI 10.1186/s13002-016-0089-8 REVIEW Open Access Traditional use and management of NTFPs in Kangchenjunga Landscape: implications for conservation and livelihoods Yadav Uprety1*, Ram C. Poudel2, Janita Gurung3, Nakul Chettri3 and Ram P. Chaudhary1,4 Abstract Non-timber Forest Products (NTFPs), an important provisioning ecosystem services, are recognized for their contribution in rural livelihoods and forest conservation. Effective management through sustainable harvesting and market driven commercialization are two contrasting aspects that are bringing challenges in development of NTFPs sector. Identifying potential species having market value, conducting value chain analyses, and sustainable management of NTFPs need analysis of their use patterns by communities and trends at a regional scale. We analyzed use patterns, trends, and challenges in traditional use and management of NTFPs in the southern slope of Kangchenjunga Landscape, Eastern Himalaya and discussed potential implications for conservation and livelihoods. A total of 739 species of NTFPs used by the local people of Kangchenjunga Landscape were reported in the reviewed literature. Of these, the highest number of NTFPs was documented from India (377 species), followed by Nepal (363) and Bhutan (245). Though the reported species were used for 24 different purposes, medicinal and edible plants were the most frequently used NTFP categories in the landscape. Medicinal plants were used in 27 major ailment categories, with the highest number of species being used for gastro-intestinal disorders. Though the Kangchenjunga Landscape harbors many potential NTFPs, trade of NTFPs was found to be nominal indicating lack of commercialization due to limited market information.
    [Show full text]
  • Asteraceae Is One of the Largest Families of Flowering Plants Which Has Not Been Revised for the Flora Malesiana (Ross 1993)
    BIOTROPIA NO. 19, 2002 : 65 - 84 NOTES ON THE ASTERACEAE OF SUMATERA SRI SUDARMIYATI TJITROSOEDIRDJO Dept. of Biology, Faculty of Science and Mathematics, Bogor Agricultural University, Jl. Raya Pajajaran, Bogor and South East Asian Regional Center for Tropical Biology (SEAMEO BIOTROP) P.O. Box 116, Bogor, Indonesia. ABSTRACT An account of the tribe composition, endemic taxa, comparison with adjacent areas and weedy Asteraceae of Sumatera is given. Based on the records of January 2000, there are 133 species of 74 genera in 11 tribes. The tribe Heliantheae is the largest, with 28% of the total number of the genera, followed by Astereae with 15%, Inuleae 12%, Senecioneae 10%, Anthemideae, Eupatorieae and Lactuceae 8%, the other tribes are represented by 4% or less. The most diverse genus is Blumea with 14 species. Other genera are only represented by 10 species or less, usually 4, or 3, or 2, and mostly by 1 species only. Thirty nine or about 53% are exotic genera and the native ones are less than half of the total number of the genera. In terms of indigenous and endemic species, Sumatera is richer than Java. There are 1 genus, 7 species and 2 varieties of Asteraceae endemic to Sumatera. A number of 43 important weed species were introduced from Tropical America, Africa, Asia and Europe. Among these Chromolaena odorata and Mikania micrantha are reported as the most noxious ones. List of the genera and species recorded in Sumatera is provided in this paper. Key words : Asteraceae/Sumatera/compositions/endemic species/distribution/weedy Asteraceae INTRODUCTION Asteraceae is one of the largest families of flowering plants which has not been revised for the Flora Malesiana (Ross 1993).
    [Show full text]
  • Exploring Evolutionary and Chemical Space Using Chemoinformatic Tools and Traditional Methods in Pharmacognosy
    Digital Comprehensive Summaries of Uppsala Dissertations Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 282 from the Faculty of Pharmacy 282 Exploring evolutionary Exploring evolutionary and chemical space using and chemical space using chemoinformatic tools chemoinformatic tools and traditional methods in and traditional methods in pharmacognosy pharmacognosy ASTRID HENZ RYEN ASTRID HENZ RYEN ACTA ACTA UNIVERSITATIS UNIVERSITATIS UPSALIENSIS ISSN 1651-6192 UPSALIENSIS ISSN 1651-6192 UPPSALA ISBN 978-91-513-0843-2 UPPSALA ISBN 978-91-513-0843-2 2020 urn:nbn:se:uu:diva-399068 2020 urn:nbn:se:uu:diva-399068 Dissertation presented at Uppsala University to be publicly examined in C4:305, BMC, Dissertation presented at Uppsala University to be publicly examined in C4:305, BMC, Husargatan 3, Uppsala, Friday, 14 February 2020 at 09:15 for the degree of Doctor of Husargatan 3, Uppsala, Friday, 14 February 2020 at 09:15 for the degree of Doctor of Philosophy (Faculty of Pharmacy). The examination will be conducted in English. Faculty Philosophy (Faculty of Pharmacy). The examination will be conducted in English. Faculty examiner: Associate Professor Fernando B. Da Costa (School of Pharmaceutical Sciences of examiner: Associate Professor Fernando B. Da Costa (School of Pharmaceutical Sciences of Ribeiraõ Preto, University of Saõ Paulo). Ribeiraõ Preto, University of Saõ Paulo). Abstract Abstract Henz Ryen, A. 2020. Exploring evolutionary and chemical space using chemoinformatic Henz Ryen, A. 2020. Exploring evolutionary and chemical space using chemoinformatic tools and traditional methods in pharmacognosy. Digital Comprehensive Summaries of tools and traditional methods in pharmacognosy. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 282.
    [Show full text]