Catalog # Aliquot Size

F13-911-05 3 x 5 nmol F13-911-20 3 x 20 nmol F13-911-50 3 x 50 nmol

FLT4 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # F13-911

Lot # Z2037-49

Specificity Formulation FLT4 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human FLT4 expression. shipped at room temperature.

Product Description Reconstitution Protocol FLT4 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human FLT4 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002020. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot VEGFR3; FLT41; LMPH1A; PCL 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or FGFR1 (FLT2), Active F04-11G below -20oC. After resuspension, siRNA stock solutions ≥2 FLT1, Active F11-11G µM can undergo up to 50 freeze-thaw cycles without FLT3 Mutant (D835Y), Active F12-12BG significant degradation. For long-term storage, it is FLT3, Active F12-11G recommended that the siRNA is stored at -70oC. For most FLT4, Active F13-11G favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background FLT4 or fms-related 4 is a tyrosine kinase for vascular endothelial growth factors C and D and highly expressed in lymphangiogenesis and FLT4 siRNA Set I siRNA duplexes targeted against three exon regions maintenance of the lymphatic endothelium. FLT4 is a marker for lymphatic vessels and some high endothelial Catalog Number F13-911 venules in human adult tissues and also supported the Specific Lot Number Z2037-49 theory of the venous origin of lymphatic vessels (1). FLT4 Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol plays an essential role in the development of the Format Lyophilized powder embryonic cardiovascular system before the emergence Stability 1yr at -70oC from date of shipment of the lymphatic vessels (2). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized References and resuspended siRNAs are stored at 1. Kaipainen, A. et.al: Expression of the fms-like tyrosine or below -20oC. After resuspension, kinase 4 gene becomes restricted to lymphatic siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles endothelium during development. Proc. Nat. Acad. without significant degradation. For Sci. 92: 3566-3570, 1995. long-term storage, it is recommended 2. Dumont, D. J. et..al: Cardiovascular failure in mouse that the siRNA is stored at -70oC. For most favorable performance, avoid embryos deficient in VEGF receptor-3. Science 282: repeated handling and multiple 946-949, 1998. freeze/thaw cycles.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.