Leader in Biomolecular Solutions for Life Science ACACB Knockout 293T Cell Line, Homozygous

Catalog No.: RM01904

Basic Information Background

Catalog No. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin- RM01904 containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate- limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by Category means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate- Cell Line limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008] Description ACACB Knockout 293T Cell Line is engineered from HeLa cell line with Gene-Editing Technology. Allele-1:1bp insertion in exon1 Allele-2:94bp deletion in exon1

Gene Information Product Information

Gene Symbol Parental Cell line Amount Genotype ACACB 293T 1~5x106 cells/vial Homozygous knockout

Species Packaging Human 1 vial parental cell line and 1 vial knockout cell line

Gene ID Shipping Conditions 32 Dry ice

Swiss Prot Storage O00763 Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.

Synonyms Protocol ACC2, ACCB, HACC275 Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum

and 100U penicillin-streptomycin, at 37℃ with 5% CO2 condition. 1. Thaw the vial in 37℃ water bath ,and shake it to melt as soon as possible. Contact 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 400-999-6126 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. [email protected] 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO2. www.abclonal.com.cn 7. A subcultivation ratio of 1:2-1:4 is recommended. Sequencing data

Genome sequence analysis of PCR products from parental (WT) and ACACB knockout (KO) 293T cells, using sanger sequencing.

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