ACACB Knockout 293T Cell Lysate

Leader in Biomolecular Solutions for Life Science ACACB Knockout 293T Cell Lysate

Catalog No.: RM02031

Basic Information Background

Catalog No. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin- RM02031 containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate- limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by Category means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate- Cell Lysate limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008] Description ACACB Knockout 293T Cell Line is engineered from 293T cell line with CRISPR/Cas9 technology. Allele-1:1bp insertion in exon1 Allele-2:94bp deletion in exon1

Gene Information Product Information

Gene Symbol Parental Cell line Amount Genotype ACACB 293T 50μL, 2μg/μL. Homozygous knockout

Species Packaging Human 1 vial parental cell Lysate and 1 vial knockout cell Lysate

Gene ID Shipping Conditions 32 4℃

Swiss Prot Application O00763 Western Blot

Synonyms Storage ACC2, ACCB, HACC275 Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.

Protocol Contact To be used as WB control. Lysate is supplied in 1× SDS sample buﬀer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). 400-999-6126 Lysate should be boiled for 3 - 5 minutes before loading onto gel.

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www.abclonal.com.cn Sequencing data

Genome sequence analysis of PCR products from parental (WT) and ACACB knockout (KO) 293T cells, using sanger sequencing.