How Spiroplasma Ribosome-Inactivating
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The DNA Virus Invertebrate Iridescent Virus 6 Is a Target of the Drosophila Rnai Machinery
The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery Alfred W. Bronkhorsta,1, Koen W. R. van Cleefa,1, Nicolas Vodovarb,2, Ikbal_ Agah Ince_ c,d,e, Hervé Blancb, Just M. Vlakc, Maria-Carla Salehb,3, and Ronald P. van Rija,3 aDepartment of Medical Microbiology, Nijmegen Centre for Molecular Life Sciences, Nijmegen Institute for Infection, Inflammation, and Immunity, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands; bViruses and RNA Interference Group, Institut Pasteur, Centre National de la Recherche Scientifique, Unité de Recherche Associée 3015, 75015 Paris, France; cLaboratory of Virology, Wageningen University, 6708 PB Wageningen, The Netherlands; dDepartment of Genetics and Bioengineering, Yeditepe University, Istanbul 34755, Turkey; and eDepartment of Biosystems Engineering, Faculty of Engineering, Giresun University, Giresun 28100, Turkey Edited by Peter Palese, Mount Sinai School of Medicine, New York, NY, and approved October 19, 2012 (received for review April 28, 2012) RNA viruses in insects are targets of an RNA interference (RNAi)- sequently, are hypersensitive to virus infection and succumb more based antiviral immune response, in which viral replication inter- rapidly than their wild-type (WT) controls (11–14). mediates or viral dsRNA genomes are processed by Dicer-2 (Dcr-2) Small RNA cloning and next-generation sequencing provide into viral small interfering RNAs (vsiRNAs). Whether dsDNA virus detailed insights into vsiRNA biogenesis. In several studies in infections are controlled by the RNAi pathway remains to be insects, the polarity of the vsiRNA population deviates strongly determined. Here, we analyzed the role of RNAi in DNA virus from the highly skewed distribution of positive strand (+) over infection using Drosophila melanogaster infected with Invertebrate negative (−) viral RNAs that is generally observed in (+) RNA iridescent virus 6 (IIV-6) as a model. -
Wolbachia-Driven Selective Sweep in a Range Expanding Insect Species
Wolbachia-driven selective sweep in a range expanding insect species Junchen Deng Lunds Universitet Giacomo Assandri Istituto Superiore per la Protezione e la Ricerca Ambientale Pallavi Chauhan Lunds Universitet Ryo Futahashi Trukuba Andrea Galimberti University of Milano–Bicocca: Universita degli Studi di Milano-Bicocca Bengt Hansson Lunds Universitet Lesley Lancaster University of Aberdeen Yuma Takahashi Chiba University graduate school of science Erik I Svensson Lund University: Lunds Universitet Anne Duplouy ( anne.duplouy@helsinki. ) University of Helsinki: Helsingin Yliopisto https://orcid.org/0000-0002-7147-5199 Research article Keywords: Endosymbiosis, phylogeography, damsely, mitochondria, genetic diversity Posted Date: February 24th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-150504/v3 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/24 Abstract Background Evolutionary processes can cause strong spatial genetic signatures, such as local loss of genetic diversity, or conicting histories from mitochondrial versus nuclear markers. Investigating these genetic patterns is important, as they may reveal obscured processes and players. The maternally inherited bacterium Wolbachia is among the most widespread symbionts in insects. Wolbachia typically spreads within host species by conferring direct tness benets, or by manipulating its host reproduction to favour infected over uninfected females. Under sucient selective advantage, the mitochondrial haplotype associated with the favoured symbiotic strains will spread (i.e. hitchhike), resulting in low mitochondrial genetic variation across the host species range. The common bluetail damsely (Ischnura elegans: van der Linden, 1820) has recently emerged as a model organism of the genetics and genomic signatures of range expansion during climate change. -
Metazoan Ribosome Inactivating Protein Encoding Genes Acquired by Horizontal Gene Transfer Received: 30 September 2016 Walter J
www.nature.com/scientificreports OPEN Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer Received: 30 September 2016 Walter J. Lapadula1, Paula L. Marcet2, María L. Mascotti1, M. Virginia Sanchez-Puerta3 & Accepted: 5 April 2017 Maximiliano Juri Ayub1 Published: xx xx xxxx Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first timein silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms. Ribosome inactivating proteins (RIPs, EC 3.2.2.22) irreversibly modify ribosomes through the depurination of an adenine residue in the conserved alpha-sarcin/ricin loop of 28S rRNA1–4. This modification prevents the binding of elongation factor 2 to the ribosome, arresting protein synthesis5, 6. The occurrence of RIP genes has been exper- imentally confirmed in a wide range of plant taxa, as well as in several species of Gram positive and Gram negative bacteria7–9. -
Complete Genomes of Two Dipteran-Associated Spiroplasmas Provided Insights Into the Origin, Dynamics, and Impacts of Viral Invasion in Spiroplasma
GBE Complete Genomes of Two Dipteran-Associated Spiroplasmas Provided Insights into the Origin, Dynamics, and Impacts of Viral Invasion in Spiroplasma Chuan Ku1,Wen-SuiLo1,2,3, Ling-Ling Chen1, and Chih-Horng Kuo1,2,4,* 1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 2Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung Hsing University and Academia Sinica, Taipei, Taiwan 3Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan 4Biotechnology Center, National Chung Hsing University, Taichung, Taiwan *Corresponding author: E-mail: [email protected]. Accepted: May 21, 2013 Data deposition: The genome sequences reported in this study have been deposited at DDBJ/EMBL/GenBank under the accessions CP005077 and CP005078. Abstract Spiroplasma is a genus of wall-less, low-GC, Gram-positive bacteria with helical morphology. As commensals or pathogens of plants, insects, ticks, or crustaceans, they are closely related with mycoplasmas and form a monophyletic group (Spiroplasma– Entomoplasmataceae–Mycoides) with Mycoplasma mycoides and its relatives. In this study, we report the complete genome sequences of Spiroplasma chrysopicola and S. syrphidicola from the Chrysopicola clade. These species form the sister group to the Citri clade, which includes several well-known pathogenic spiroplasmas. Surprisingly, these two newly available genomes from the Chrysopicola clade contain no plectroviral genes, which were found to be highly repetitive in the previously sequenced genomes from the Citri clade. Based on the genome alignment and patterns of GC-skew, these two Chrysopicola genomes appear to be relatively stable, rather than being highly rearranged as those from the Citri clade. -
Thermal Sensitivity of the Spiroplasma-Drosophila Hydei Protective Symbiosis: the Best of 2 Climes, the Worst of Climes
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.30.070938; this version posted May 2, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Thermal sensitivity of the Spiroplasma-Drosophila hydei protective symbiosis: The best of 2 climes, the worst of climes. 3 4 Chris Corbin, Jordan E. Jones, Ewa Chrostek, Andy Fenton & Gregory D. D. Hurst* 5 6 Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Crown 7 Street, Liverpool L69 7ZB, UK 8 9 * For correspondence: [email protected] 10 11 Short title: Thermal sensitivity of a protective symbiosis 12 13 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.04.30.070938; this version posted May 2, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 14 Abstract 15 16 The outcome of natural enemy attack in insects has commonly been found to be influenced 17 by the presence of protective symbionts in the host. The degree to which protection 18 functions in natural populations, however, will depend on the robustness of the phenotype 19 to variation in the abiotic environment. We studied the impact of a key environmental 20 parameter – temperature – on the efficacy of the protective effect of the symbiont 21 Spiroplasma on its host Drosophila hydei, against attack by the parasitoid wasp Leptopilina 22 heterotoma. -
Finding of Male-Killing Spiroplasma Infecting Drosophila Melanogaster in Africa Implies Transatlantic Migration of This Endosymbiont
Heredity (2006) 97, 27–32 & 2006 Nature Publishing Group All rights reserved 0018-067X/06 $30.00 www.nature.com/hdy Finding of male-killing Spiroplasma infecting Drosophila melanogaster in Africa implies transatlantic migration of this endosymbiont JE Pool, A Wong and CF Aquadro Department of Molecular Biology and Genetics, Cornell University, 233 Biotechnology Building, Ithaca, NY 14853, USA We report the identification of male-killing Spiroplasma in a efficiency appear to increase with female age, and we note wild-caught female Drosophila melanogaster from Uganda, that males born in sex ratio broods display much lower the first such infection to be found in this species outside survivorship than their female siblings. DNA sequence of South America. Among 38 female flies collected from comparisons at three loci suggest that this Spiroplasma Namulonge, Uganda in April, 2005, one produced a total strain is closely related to the male-killing strain previously of 41 female offspring but no males. PCR testing of found to infect D. melanogaster in Brazil, although part of subsequent generations revealed that females retaining one locus appears to show a recombinant history. Implica- Spiroplasma infection continued to produce a large excess tions for the origin and history of male-killing Spiroplasma in of female progeny, while females that had lost Spiroplasma D. melanogaster are discussed. produced offspring with normal sex ratios. Consistent with Heredity (2006) 97, 27–32. doi:10.1038/sj.hdy.6800830; earlier work, we find that male-killing and transmission published online 10 May 2006 Keywords: Spiroplasma; male-killing; Drosophila melanogaster; Africa; migration Introduction few hundred years (David and Capy, 1988). -
Loss of Reproductive Parasitism Following Transfer of Male-Killing Wolbachia to Drosophila Melanogaster and Drosophila Simulans
Heredity (2012) 109, 306–312 & 2012 Macmillan Publishers Limited All rights reserved 0018-067X/12 www.nature.com/hdy ORIGINAL ARTICLE Loss of reproductive parasitism following transfer of male-killing Wolbachia to Drosophila melanogaster and Drosophila simulans Z Veneti1,2,8, S Zabalou3,8, G Papafotiou4, C Paraskevopoulos5, S Pattas3, I Livadaras1, G Markakis3, JK Herren6,7, J Jaenike6 and K Bourtzis4,5,9 Wolbachia manipulate insect host biology through a variety of means that result in increased production of infected females, enhancing its own transmission. A Wolbachia strain (wInn) naturally infecting Drosophila innubila induces male killing, while native strains of D. melanogaster and D. simulans usually induce cytoplasmic incompatibility (CI). In this study, we transferred wInn to D. melanogaster and D. simulans by embryonic microinjection, expecting conservation of the male-killing phenotype to the novel hosts, which are more suitable for genetic analysis. In contrast to our expectations, there was no effect on offspring sex ratio. Furthermore, no CI was observed in the transinfected flies. Overall, transinfected D. melanogaster lines displayed lower transmission rate and lower densities of Wolbachia than transinfected D. simulans lines, in which established infections were transmitted with near-perfect fidelity. In D. simulans, strain wInn had no effect on fecundity and egg-to-adult development. Surprisingly, one of the two transinfected lines tested showed increased longevity. We discuss our results in the context of host-symbiont co-evolution and the potential of symbionts to invade novel host species. Heredity (2012) 109, 306–312; doi:10.1038/hdy.2012.43; published online 15 August 2012 Keywords: Wolbachia; symbiosis; male-killing; cytoplasmic incompatibility INTRODUCTION prevalence of infection. -
Selection and Demography Shape Genomic Variation in a ‘Sky Island’ Species
bioRxiv preprint doi: https://doi.org/10.1101/2020.05.14.096008; this version posted May 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Selection and demography shape genomic variation in a ‘Sky Island’ species Tom Hill1*, Robert L. Unckless1 1. 4055 Haworth Hall, The Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045. Email: [email protected] * Corresponding author Keywords: Drosophila innubila, local adaptation, phylogeography, inversions 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.05.14.096008; this version posted May 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Abstract 2 Over time, populations of species can expand, contract, and become isolated, creating subpopulations that 3 can adapt to local conditions. Understanding how species adapt following these changes is of great interest, 4 especially as the current climate crisis has caused range shifts for many species. Here, we characterize how 5 Drosophila innubila came to inhabit and adapt to its current range: mountain forests in southwestern USA 6 separated by large expanses of desert. Using population genomic data from more than 300 wild-caught 7 individuals, we examine four distinct populations to determine their population history in these mountain- 8 forests, looking for signatures of local adaptation to establish a genomic model for this spatially-distributed 9 system with a well understood ecology. -
The Wall-Less Bacterium Spiroplasma Poulsonii Builds a Polymeric
bioRxiv preprint doi: https://doi.org/10.1101/2021.06.08.447548; this version posted June 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 The wall-less bacterium Spiroplasma poulsonii builds a polymeric 2 cytoskeleton composed of interacting MreB isoforms 3 Florent Masson1*, Xavier Pierrat1,2, Bruno Lemaitre1, Alexandre Persat1,2* 4 5 1Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 6 Lausanne, Switzerland 7 2Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne 8 (EPFL), Lausanne, Switzerland 9 10 *Corresponding author: 11 Phone number: +41 21 693 12 51 12 Email address: [email protected] ; [email protected] 13 14 ORCID numbers: 15 FM: 0000-0002-5828-2616 16 XP: 0000-0002-3522-2514 17 BL: 0000-0001-7970-1667 18 AP: 0000-0001-8426-8255 19 20 Running title: MreB isoforms of Spiroplasma 21 22 Keywords: MreB, cytoskeleton, Spiroplasma, Mollicutes 23 24 Classification: Biological sciences, Microbiology. 25 26 This PDF file includes: 27 Main Text 28 Figures 1 to 4 bioRxiv preprint doi: https://doi.org/10.1101/2021.06.08.447548; this version posted June 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. -
Duke University Dissertation Template
Parental Conflict, Parent of Origin Effects, and the Evolution of Hybrid Seed Failure in Mimulus by Jennifer M. Coughlan Department of Biology Duke University Date:_______________________ Approved: ___________________________ John Willis, PhD, Supervisor ___________________________ Kathleen Donohue, PhD ___________________________ Mark Rausher, PhD ___________________________ Mohamed Noor, PhD ___________________________ Thomas Mitchell-Olds Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biology in the Graduate School of Duke University 2018 i v ABSTRACT Parental Conflict, Parent of Origin Effects, and the Evolution of Hybrid Seed Failure in Mimulus by Jennifer M. Coughlan Department of Biology Duke University Date:_______________________ Approved: ___________________________ John Willis, PhD, Supervisor ___________________________ Kathleen Donohue, PhD ___________________________ Mark Rausher, PhD ___________________________ Mohamed Noor, PhD ___________________________ Thomas Mitchell-Olds An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biology in the Graduate School of Duke University 2018 i v Copyright by Jennifer M. Coughlan 2018 Abstract The earth is home to roughly 9 million eukaryotic species. The formation and maintenance of this diversity requires the accumulation of barriers to reproduction. One of the most common post-zygotic barriers in plants is hybrid seed inviability (HSI). Despite its commonality, we know relatively little about the genetic mechanisms and evolutionary forces which are responsible for this barrier, particularly in naturally co- occurring species. Here I tested the role of parental conflict in HSI between co-occurring monkey flowers in the M. guttatus species complex. I assessed the strength and directionality of HSI within and between phenotypically described perennial variants of the M. -
1 Transposable Element Dynamics Are Consistent Across The
bioRxiv preprint doi: https://doi.org/10.1101/651059; this version posted July 26, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Transposable element dynamics are consistent across the Drosophila phylogeny, despite drastically differing content Tom Hill1* 1. 4012 Haworth Hall, The Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045. Email: [email protected] * Corresponding author Keywords: Transposable elements, Drosophila, fitness. 1 bioRxiv preprint doi: https://doi.org/10.1101/651059; this version posted July 26, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Abstract 2 Background: The evolutionary dynamics of transposable elements (TEs) vary across the tree of 3 life and even between closely related species with similar ecologies. In Drosophila, most of the 4 focus on TE dynamics has been completed in Drosophila melanogaster and the overall pattern 5 indicates that TEs show an excess of low frequency insertions, consistent with their frequent turn 6 over and high fitness cost in the genome. Outside of D. melanogaster, insertions in the species 7 Drosophila algonquin, suggests that this situation may not be universal, even within Drosophila. 8 Here we test whether the pattern observed in D. -
Isolation of a Natural DNA Virus of Drosophila Melanogaster, and Characterisation of Host Resistance and Immune Responses
Edinburgh Research Explorer Isolation of a natural DNA virus of Drosophila melanogaster, and characterisation of host resistance and immune responses Citation for published version: Palmer, WH, Medd, NC, Beard, PM & Obbard, D 2018, 'Isolation of a natural DNA virus of Drosophila melanogaster, and characterisation of host resistance and immune responses', Plos Pathogens, vol. 14, no. 6. https://doi.org/10.1371/journal.ppat.1007050 Digital Object Identifier (DOI): 10.1371/journal.ppat.1007050 Link: Link to publication record in Edinburgh Research Explorer Document Version: Publisher's PDF, also known as Version of record Published In: Plos Pathogens Publisher Rights Statement: © 2018 Palmer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 11. May. 2020 RESEARCH ARTICLE Isolation of a natural DNA virus of Drosophila melanogaster, and characterisation of host resistance and immune responses William H.