Dynein/Dynactin Is Necessary for Anterograde Transport of Mbp
Dynein/dynactin is necessary for anterograde transport PNAS PLUS of Mbp mRNA in oligodendrocytes and for myelination in vivo Amy L. Herberta,1, Meng-meng Fub,1,2, Catherine M. Drerupc,3, Ryan S. Graya,4, Breanne L. Hartya,5, Sarah D. Ackermana,6, Thomas O’Reilly-Pold, Stephen L. Johnsond, Alex V. Nechiporukc, Ben A. Barresb,2, and Kelly R. Monka,2,5 aDepartment of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110; bDepartment of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305; cDepartment of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, OR 97239; and dDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110 Contributed by Ben A. Barres, August 25, 2017 (sent for review February 26, 2017; reviewed by Bruce Appel, Roman J. Giger, and Jeffery L. Twiss) Oligodendrocytes in the central nervous system produce myelin, a sheaths is stimulated by Fyn kinase, which is phosphorylated in lipid-rich, multilamellar sheath that surrounds axons and promotes response to axonal electrical activity (6–8). In addition, MBP acts the rapid propagation of action potentials. A critical component of as an important spatial and temporal regulator of myelination, by myelin is myelin basic protein (MBP), expression of which requires triggering disassembly of the actin cytoskeleton to promote initi- anterograde mRNA transport followed by local translation at the ation of myelin membrane wrapping (9, 10). developing myelin sheath. Although the anterograde motor Classic experiments in cultured oligodendrocytes demon- kinesin KIF1B is involved in mbp mRNA transport in zebrafish, it strated that Mbp mRNA trafficking in the anterograde direction is not entirely clear how mbp transport is regulated.
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