Observed Termite Activity in Sector 2 in 2009
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University of São Paulo “Luiz de Queiroz” College of Agriculture Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth Natasha Sant´Anna Iwanicki Thesis presented to obtain the degreee of Doctor in Science. Area: Entomology Piracicaba 2020 UNIVERSITY OF COPENHAGEN FACULTY OF SCIENCE Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth PhD THESIS 2020 – Natasha Sant´Anna Iwanicki Natasha Sant´Anna Iwanicki Agronomic Engineer Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth Advisors: Prof. Dr. ITALO DELALIBERA JUNIOR Prof. PhD and Dr. agro JØRGEN EILENBERG Co-advisor for transcriptomic studies: Associate professor PhD HENRIK H. DE FINE LICHT Thesis presented to obtain the double-degreee of Doctor in Science of the University of São Paulo and PhD at University of Copenhagen. Area: Entomology Piracicaba 2020 2 Dados Internacionais de Catalogação na Publicação DIVISÃO DE BIBLIOTECA – DIBD/ESALQ/USP Iwanicki, Natasha Sant´Anna Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth / Natasha Sant´Anna Iwanicki. - - Piracicaba, 2020. 248 p. Tese (Doutorado) - - USP / Escola Superior de Agricultura “Luiz de Queiroz”. 1. Blastosporos 2. Fermentação líquida 3. Dimorfismo fúngico 4. Fungos entomopatogênicos I. Título 3 4 ACKNOWLEDGMENTS First, I would like to thank my supervisors, Prof. Italo Delalibera Júnior and Prof. Jørgen Eilenberg for their confidence in my potential as a student, for the opportunities they gave me and the knowledge they shared, for their guidance and friendship over these years. I also thank my co-advisors, Prof. -
Comparative Genomics of Knoxdaviesia Species in the Core Cape Subregion
Comparative genomics of Knoxdaviesia species in the Core Cape Subregion by Janneke Aylward Dissertation presented for the degree of Doctor of Philosophy in the Faculty of Science at Stellenbosch University Supervisor: Prof. Léanne L. Dreyer Co-supervisors: Dr. Francois Roets Prof. Emma T. Steenkamp Prof. Brenda D. Wingfield Prof. Michael J. Wingfield March 2017 Stellenbosch University https://scholar.sun.ac.za Declaration By submitting this dissertation electronically, I declare that the entirety of the work contained therein is my own, original work, that I am the sole author thereof (save to the extent explicitly otherwise stated), that reproduction and publication thereof by Stellenbosch University will not infringe any third party rights and that I have not previously in its entirety or in part submitted it for obtaining any qualification. Janneke Aylward Date: March 2017 Copyright © 2017 Stellenbosch University All rights reserved i Stellenbosch University https://scholar.sun.ac.za ABSTRACT Knoxdaviesia capensis and K. proteae are saprotrophic fungi that inhabit the seed cones (infructescences) of Protea plants in the Core Cape Subregion (CCR) of South Africa. Arthropods, implicated in the pollination of Protea species, disperse these native fungi from infructescences to young flower heads (inflorescences). Knoxdaviesia proteae is a specialist restricted to one Protea species, while the generalist K. capensis occupies a range of Protea species. Within young flower heads, Knoxdaviesia species grow vegetatively, but switch to sexual reproduction once flower heads mature into enclosed infructescences. Nectar becomes depleted and infructescences are colonised by numerous other organisms, including the arthropod vectors of the fungi. The aim of this dissertation was to study the ecology of K. -
Horizontal Gene Transfer Allowed the Emergence of Broad Host Range Entomopathogens
Horizontal gene transfer allowed the emergence of broad host range entomopathogens Qiangqiang Zhanga,1, Xiaoxuan Chena,1, Chuan Xua, Hong Zhaoa, Xing Zhanga, Guohong Zenga, Ying Qiana, Ran Liua, Na Guoa, Wubin Mia, Yamin Menga, Raymond J. St. Legerb, and Weiguo Fanga,2 aMOE Key Laboratory of Biosystems Homeostasis & Protection, College of Life Science, Zhejiang University, Hangzhou 310058, China; and bDepartment of Entomology, University of Maryland, College Park, MD 20742 Edited by Thomas A. Richards, University of Exeter, Exeter, United Kingdom, and accepted by Editorial Board Member W. F. Doolittle March 10, 2019 (received for review September 24, 2018) The emergence of new pathogenic fungi has profoundly impacted Systematic studies have shown that horizontal gene transfer global biota, but the underlying mechanisms behind host shifts (HGT, i.e., the movement of genetic material between distant or- remain largely unknown. The endophytic insect pathogen Metarhizium ganisms) is prevalent in prokaryotes, in which it serves as an im- robertsii evolved from fungi that were plant associates, and entomo- portant mechanism for the emergence of new bacterial pathogens. pathogenicity is a more recently acquired adaptation. Here we report However, the extent to which HGT contributes to the evolution of that the broad host-range entomopathogen M. robertsii has 18 genes eukaryotic pathogens is largely unknown (12), in large measure that are derived via horizontal gene transfer (HGT). The necessity of because of a lack of systematic functional characterization of HGTs degrading insect cuticle served as a major selective pressure to retain (13). In this study, we report that HGT of 18 genes, many involved these genes, as 12 are up-regulated during penetration; 6 were con- in cuticle penetration, was a key mechanism in the emergence of firmed to have a role in penetration, and their collective actions are entomopathogenicity in Metarhizium, and that acquisition and/or indispensable for infection. -
Interaction Between Metarhizium Anisopliae and Its Host, the Subterranean Termite Coptotermes Curvignathus During the Infection Process
biology Article Interaction between Metarhizium anisopliae and Its Host, the Subterranean Termite Coptotermes curvignathus during the Infection Process Samsuddin Ahmad Syazwan 1,2 , Shiou Yih Lee 1, Ahmad Said Sajap 1, Wei Hong Lau 3, Dzolkhifli Omar 3 and Rozi Mohamed 1,* 1 Department of Forest Science and Biodiversity, Faculty of Forestry and Environment, Universiti Putra Malaysia, Serdang 43400, Malaysia; [email protected] (S.A.S.); [email protected] (S.Y.L.); [email protected] (A.S.S.) 2 Mycology and Pathology Branch, Forest Biodiversity Division, Forest Research Institute Malaysia (FRIM), Kepong 52109, Malaysia 3 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Malaysia; [email protected] (W.H.L.); zolkifl[email protected] (D.O.) * Correspondence: [email protected]; Tel.: +60-397-697-183 Simple Summary: The use of Metarhizium anisopliae as a biological control of insect pests has been experimented in the laboratory as well as in field trials. This includes against the termite Coptotermes curvignathus, however the results have varying degrees of success. One reason could be due to the lack of detailed knowledge on the molecular pathogenesis of M. anisopliae. In the current study, the conidial suspension of M. anisopliae isolate PR1 was first inoculated on the C. curvignathus, after which the pathogenesis was examined using two different approaches: electron microscopy and protein expression. At the initiation stage, the progression observed and documented including Citation: Syazwan, S.A.; Lee, S.Y.; adhesion, germination, and penetration of the fungus on the cuticle within 24 h after inoculation. Sajap, A.S.; Lau, W.H.; Omar, D.; Later, this was followed by colonization and spreading of the fungus at the cellular level. -
Technological Advances to Address Current Issues in Entomology: 2020 Student Debates
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Entomology papers Museum, University of Nebraska State 2-28-2021 Technological Advances to Address Current Issues in Entomology: 2020 Student Debates Lina Bernaola Louisiana State University at Baton Rouge Molly Darlington University of Nebraska—Lincoln, [email protected] Kadie Britt Virginia Polytechnic Institute and State University Patricia Prade University of Florida Morgan Roth Virginia Polytechnic Institute and State University See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/entomologypapers Bernaola, Lina; Darlington, Molly; Britt, Kadie; Prade, Patricia; Roth, Morgan; Pekarcik, Adrian; Boone, Michelle; Ricke, Dylan; Tran, Anh; King, Joanie; Carruthers, Kelly; Thompson, Morgan; Ternest, John J.; Anderson, Sarah E.; Gula, Scott W.; Hauri, Kayleigh C.; Pecenka, Jacob R.; Grover, Sajjan; Puri, Heena; and Vakil, Surabhi Gupta, "Technological Advances to Address Current Issues in Entomology: 2020 Student Debates" (2021). Entomology papers. 171. https://digitalcommons.unl.edu/entomologypapers/171 This Article is brought to you for free and open access by the Museum, University of Nebraska State at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Entomology papers by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Lina Bernaola, Molly Darlington, Kadie Britt, Patricia Prade, Morgan Roth, Adrian Pekarcik, Michelle -
Oviposition Behavior of the Female Coconut Rhinoceros Beetle, Oryctes Rhinoceros
Oviposition Behavior of the Female Coconut Rhinoceros Beetle, Oryctes rhinoceros (Coleoptera: Scarabaeidae) A THESIS SUBMITTED TO THE GRADUATE DIVISION OF THE UNIVERSITY OF HAWAI‘I AT MĀNOA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTERS IN SCIENCE IN ENTOMOLOGY DECEMBER 2017 By Megan E. Manley Thesis Committee: Helen Spafford, Co-chairperson Michael Melzer, Co-chairperson Mark Wright Keywords: Coconut rhinoceros beetle, Oryctes rhinoceros, oviposition DEDICATION To my mother, Marilyn Noble Manley, for sacrificing so much to raise me and my sisters and for showing us what a strong woman is. Thank you for never giving up on me and helping me to grow into a woman I know Lolo would be proud of. I love you Mom. AKNOWLEDGEMENTS This thesis was completed with tremendous support from my committee co-chairs, Dr. Helen Spafford and Dr. Michael Melzer, as well as my committee member Dr. Mark Wright. I would like to thank them for giving me this opportunity, and without their constant guidance and advice, I would not have had such a great experience throughout my journey to completing my thesis. I would like to give a special thank you to Dr. Helen Spafford for being an exceptional human being, understanding me as a person, and inspiring me as a woman in science. I also extend an immense amount of gratitude to Dr. Shizu Watanabe for being there for me personally and professionally, and for acting as a true mentor; you are greatly appreciated. I would like to thank the CRB Response Team, Dr. Keith Weiser, Tomie Vowell, Nelson Masang, Scott Appelbaum, Allie Kong, Matthew Kellar, and Brandi Adams for working on the beetle colony with me and helping me with numerous tasks pertaining to my research. -
Diversity Within the Entomopathogenic Fungal Species Metarhizium Flavoviride Associated with Agricultural Crops in Denmark Chad A
Keyser et al. BMC Microbiology (2015) 15:249 DOI 10.1186/s12866-015-0589-z RESEARCH ARTICLE Open Access Diversity within the entomopathogenic fungal species Metarhizium flavoviride associated with agricultural crops in Denmark Chad A. Keyser, Henrik H. De Fine Licht, Bernhardt M. Steinwender and Nicolai V. Meyling* Abstract Background: Knowledge of the natural occurrence and community structure of entomopathogenic fungi is important to understand their ecological role. Species of the genus Metarhizium are widespread in soils and have recently been reported to associate with plant roots, but the species M. flavoviride has so far received little attention and intra-specific diversity among isolate collections has never been assessed. In the present study M. flavoviride was found to be abundant among Metarhizium spp. isolates obtained from roots and root-associated soil of winter wheat, winter oilseed rape and neighboring uncultivated pastures at three geographically separated locations in Denmark. The objective was therefore to evaluate molecular diversity and resolve the potential population structure of M. flavoviride. Results: Of the 132 Metarhizium isolates obtained, morphological data and DNA sequencing revealed that 118 belonged to M. flavoviride,13toM. brunneum and one to M. majus. Further characterization of intraspecific variability within M. flavoviride was done by using amplified fragment length polymorphisms (AFLP) to evaluate diversity and potential crop and/or locality associations. A high level of diversity among the M. flavoviride isolates was observed, indicating that the isolates were not of the same clonal origin, and that certain haplotypes were shared with M. flavoviride isolates from other countries. However, no population structure in the form of significant haplotype groupings or habitat associations could be determined among the 118 analyzed M. -
Fungal Pathogens Occurring on <I>Orthopterida</I> in Thailand
Persoonia 44, 2020: 140–160 ISSN (Online) 1878-9080 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE https://doi.org/10.3767/persoonia.2020.44.06 Fungal pathogens occurring on Orthopterida in Thailand D. Thanakitpipattana1, K. Tasanathai1, S. Mongkolsamrit1, A. Khonsanit1, S. Lamlertthon2, J.J. Luangsa-ard1 Key words Abstract Two new fungal genera and six species occurring on insects in the orders Orthoptera and Phasmatodea (superorder Orthopterida) were discovered that are distributed across three families in the Hypocreales. Sixty-seven Clavicipitaceae sequences generated in this study were used in a multi-locus phylogenetic study comprising SSU, LSU, TEF, RPB1 Cordycipitaceae and RPB2 together with the nuclear intergenic region (IGR). These new taxa are introduced as Metarhizium grylli entomopathogenic fungi dicola, M. phasmatodeae, Neotorrubiella chinghridicola, Ophiocordyceps kobayasii, O. krachonicola and Petchia new taxa siamensis. Petchia siamensis shows resemblance to Cordyceps mantidicola by infecting egg cases (ootheca) of Ophiocordycipitaceae praying mantis (Mantidae) and having obovoid perithecial heads but differs in the size of its perithecia and ascospore taxonomy shape. Two new species in the Metarhizium cluster belonging to the M. anisopliae complex are described that differ from known species with respect to phialide size, conidia and host. Neotorrubiella chinghridicola resembles Tor rubiella in the absence of a stipe and can be distinguished by the production of whole ascospores, which are not commonly found in Torrubiella (except in Torrubiella hemipterigena, which produces multiseptate, whole ascospores). Ophiocordyceps krachonicola is pathogenic to mole crickets and shows resemblance to O. nigrella, O. ravenelii and O. barnesii in having darkly pigmented stromata. Ophiocordyceps kobayasii occurs on small crickets, and is the phylogenetic sister species of taxa in the ‘sphecocephala’ clade. -
Cvckc-2017-0293-0311
Clinical Approach to Ferret Lymphoma La’Toya Latney, DVM University of Pennsylvania Philadelphia, PA Lymphoma connotes a solid-tissue tumor composed of neoplastic lymphocytes in visceral organs, skin, or lymph nodes throughout the body (Antinoff). To date, lymphoma is the most common malignant neoplasia reported in the domestic ferret at 10-15% of all neoplastic presentations in the US and Europe, and lymphoma is the third most common neoplasia of ferrets, behind adrenocortical neoplasia and insulinoma. It is documented as a spontaneous neoplasia (Mayer, Quesenberry), however there have been reports of horizontal transmission via cell and cell-free inoculation (Erdman), which suggests that there may a viral etiology, however an agent has never been reported. There is one report of Helicobacter mustelidae-associated (MALT) gastric B-cell lymphoma (Erdman), and this syndrome appears to mimic gastric B cell lymphoma caused H.pylori in humans. Ferret lymphoma can occur across a number of age groups and has no specific sex predilections. In the early literature describing the disease, ferret lymphoma was classified by age of onset and assigned distinct prognosis, i.e. the aggressive and quickly fatal juvenile onset lymphoma form and the adult chronic onset form. This generalized classification scheme has been since retracted due to new clinical reports that reveal there is no specific age and cell-type trend. Most resources characterize lymphoma by cell line, i.e. large cell, lymphoblastic lymphoma (T cell) or small cell, lymphocytic lymphoma (B cell). Finally, there are several studies that report disease based on location, which include but are not limited to multicentric lymphoma (Ferreira), cutaneous lymphoma (Xi, Rosenbaum), malignant B-cell lymphoma with Mott cell differentiation (Gupta), polyostotic lymphoma (Long), epitheliotropic gastrointestinal T-cell lymphoma (Sinclair), focal thoracolumbar spinal cord lymphoma (Ingrao), myelo-osteolytic plasmacytic lymphoma in the femur (Eshar), and gastrointestinal lymphoma (Lee). -
Current Knowledge of the Entomopathogenic Fungal Species Metarhizium flavoviride Sensu Lato and Its Potential in Sustainable Pest Control
insects Review Current Knowledge of the Entomopathogenic Fungal Species Metarhizium flavoviride Sensu Lato and Its Potential in Sustainable Pest Control Franciska Tóthné Bogdányi 1 , Renáta Petrikovszki 2 , Adalbert Balog 3, Barna Putnoky-Csicsó 3, Anita Gódor 2,János Bálint 3,* and Ferenc Tóth 2,* 1 FKF Nonprofit Zrt., Alföldi str. 7, 1081 Budapest, Hungary; [email protected] 2 Plant Protection Institute, Faculty of Agricultural and Environmental Sciences, Szent István University, Páter Károly srt. 1, 2100 Gödöll˝o,Hungary; [email protected] (R.P.); [email protected] (A.G.) 3 Department of Horticulture, Faculty of Technical and Human Sciences, Sapientia Hungarian University of Transylvania, Allea Sighis, oarei 1C, 540485 Targu Mures/Corunca, Romania; [email protected] (A.B.); [email protected] (B.P.-C.) * Correspondence: [email protected] (J.B.); [email protected] (F.T.); Tel.: +40-744-782-982 (J.B.); +36-30-5551-255 (F.T.) Received: 17 July 2019; Accepted: 31 October 2019; Published: 2 November 2019 Abstract: Fungal entomopathogens are gaining increasing attention as alternatives to chemical control of arthropod pests, and the literature on their use under different conditions and against different species keeps expanding. Our review compiles information regarding the entomopathogenic fungal species Metarhizium flavoviride (Gams and Rozsypal 1956) (Hypocreales: Clavicipitaceae) and gives account of the natural occurrences and target arthropods that can be controlled using M. flavoviride. Taxonomic problems around M. flavoviride species sensu lato are explained. Bioassays, laboratory and field studies examining the effect of fermentation, culture regimes and formulation are compiled along with studies on the effect of the fungus on target and non-target organisms and presenting the effect of management practices on the use of the fungus. -
Metarhizium Dendrolimatilis, a Novel Metarhizium Species Parasitic on Dendrolimus Sp
Mycosphere 8(1): 31–37 (2017) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/8/1/4 Copyright © Guizhou Academy of Agricultural Sciences Metarhizium dendrolimatilis, a novel Metarhizium species parasitic on Dendrolimus sp. larvae Chen WH1, 2, Han YF2, Liang JD3, Liang ZQ2 and Jin DC1 1 Institute of Entomology, College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, China 2 Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China 3Department of Microbiology, School of Basic Medical Science, Guiyang College of Traditional Chinese Medicine, Guiyang, Guizhou 550025, China Chen WH, Han YF, Liang JD, Liang ZQ, Jin DC 2017 –Metarhizium dendrolimatilis, a novel Metarhizium species parasitic on Dendrolimus sp. larvae. Mycosphere 8(1), 31–37, Doi 10.5943/mycosphere/8/1/4 Abstract A novel species of the genus Meatrhizium, Metarhizium dendrolimatilis, parasitic on Dendrolimus sp. larvae, collected in Huaxi, Guiyang, Guizhou Province, China, is described based on morphological and phylogenetic evidences. This species differs morphologically from other species in the genus by its determinate synnemata, ellipsoidal conidia, and globose phialides. The phylogenetic analyses based on four loci (EF1a, RPB1, RPB2 and TUB), strongly support the novel species designation of this fungus within the Metarhizium genus, Metarhizium dendrolimatilis sp. nov. Key words – entomopathogenic fungi – morphology – multi-gene – phylogeny Introduction The genus Metarhizium (Metschn.) Sorokin consists of a diverse group of asexual entomopathogenic fungi with a global distribution and a wide range of host insects. The organism has been used as bio pesticide (Roberts & St. Leger 2004) for mite and tick control (Maniania et al. -
Expert Consultation and Risk Assessment on the Importation and Large-Scale Use of Mycopesticides Against Locusts
Report Expert Consultation and Risk Assessment on the Importation and Large-Scale Use of Mycopesticides against Locusts Rome, 2-7 December 2001 Food and Agriculture Organization of the United Nations 2002 2 Table of Contents PART 1. INTRODUCTION ...................................................................................................................................4 Objectives of the Expert Consultation.......................................................................................................................4 Opening .....................................................................................................................................................................5 Agenda and Chair.......................................................................................................................................................5 Special Considerations ...............................................................................................................................................5 Part 2 Use of Metarhizium against Locusts and Grasshoppers..........................................................................6 Recent developments in the use of Metarhizium ..............................................................................................6 Similarity of Metarhizium isolates.............................................................................................................................7 Efficacy.......................................................................................................................................................................8