Beneficial Effects of Estrogens on Indices of Renal Damage in Uninephrectomized Shrsp Rats
J Am Soc Nephrol 15: 348–358, 2004 Beneficial Effects of Estrogens on Indices of Renal Damage in Uninephrectomized SHRsp Rats MARIE-LUISE GROSS,* MARCIN ADAMCZAK,†‡ THOMAS RABE,§ NEVIN ALI HARBI,† JAN KRTIL,† ANDREAS KOCH,* PETER HAMAR, KERSTIN AMANN,¶ and EBERHARD RITZ† Departments of *Pathology and †Internal Medicine, University of Heidelberg, Heidelberg, Germany; ‡Department of Nephrology, Endocrinology, and Metabolic Diseases, Medical University of Silesia, Katowice, Poland; §Department of Gynecological Endocrinology and Reproductive Medicine, University Women’s Hospital, Heidelberg, Germany; Institute of Pathophysiology, Semmelweis University, Budapest, Hungary; and ¶Department of Pathology, University of Erlangen, Erlangen, Germany. Abstract. Renal diseases tend to be less severe among pre- estradiol-treated animals (estradiol-treated UNX/OVX ani- menopausal female patients, compared with male patients. mals, 0.69 Ϯ 0.16; estriol-treated UNX/OVX animals, 0.21 Ϯ Experimental data on the effects of estrogens on renal damage 0.12; P Ͻ 0.05), compared with vehicle-treated animals (1.46 are controversial, and potential underlying mechanisms have Ϯ 0.09); the tubulointerstitial damage index exhibited a similar not been fully clarified. Three-month-old, female, uninephrec- pattern. The mean glomerular volume was significantly less in tomized (UNX), sham-operated or ovariectomized (OVX) estrogen-treated animals. UNX/OVX animals demonstrated SHRsp rats were left untreated or received either 17-estradiol significantly greater expression of TGF- and endothelin-1 in 3-benzoate (25 g/d) or estriol (0.02 mg/d) daily. After 3 mo, immunohistochemical, in situ hybridization, and reverse tran- indices of renal damage (glomerulosclerosis index and tubulo- scription-PCR assays. This increase was abrogated by estriol interstitial damage index) and glomerular geometric parame- but not estradiol.
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