Supplementary materials
a b
c d
Figure S1. Illumina sequence‐based biodiversity indices rarefaction curves: a—Chao 1 index, b—Pielou index, с—Shannon index, d—Simpson index.
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Table S1. Isolated strains catalogue.
Strain Primers used for amplification Primers used for sequence Taxonomical affiliation KBP.AS.110 27f + Un1492r 1100r Bacillus sp. KBP.AS.112 27f + Un1492r 1100r Bacillus sp. KBP.AS.113 27f + Un1492r 1100r Ochrobactrum sp. KBP.AS.122 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.1261 27f + Un1492r 1100r Ochrobactrum thiophenivorans KBP.AS.1262 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.1263 Identification was carried out according to phenotypic characters. Streptomyces sp. KBP.AS.1264 27f + Un1492r 1100r Ochrobactrum thiophenivorans KBP.AS.1265 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.1266 27f + Un1492r 1100r Paracoccus sp. KBP.AS.1267 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.1268 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.1269 341f + 805r 805r Stenotrophomonas sp. KBP.AS.1270 341f + 805r 805r Stenotrophomonas maltophilia KBP.AS.1271 341f + 805r 805r Enterobacter sp. KBP.AS.1272 341f + 805r 805r Stenotrophomonas maltophilia KBP.AS.1273 27f + Un1492r 1100r Ochrobactrum thiophenivorans KBP.AS.1275 27f + Un1492r 1100r Microbacterium paraoxydans KBP.AS.220 27f + Un1492r 1100r Stenotrophomonas sp. KBP.AS.230 341f + 805r 805r Micrococcus sp. KBP.AS.231 27f + Un1492r 1100r Bacillus pumilus KBP.AS.232 27f + Un1492r 1100r Micrococcus sp. KBP.AS.233 Identification was carried out according to phenotypic characters. Rhodococcus sp. KBP.AS.234 341f + 805r 805r Bacillus infantis KBP.AS.235 Identification was carried out according to phenotypic characters. Streptomyces sp. KBP.AS.236 27f + Un1492r 1100r Arthrobacter agilis KBP.AS.237 27f + Un1492r 1100r Paracoccus marcusii KBP.AS.238 27f + Un1492r 1100r Brevibacterium linens KBP.AS.239 341f + 805r 805r Micrococcus terreus KBP.AS.240 27f + Un1492r 1100r Brevibacterium sp. KBP.AS.241 341f + 805r 805r Streptomyces sp. KBP.AS.242 27f + Un1492r 1100r Microbacterium sp. KBP.AS.244 341f + 805r 805r Pseudomonas sp. KBP.AS.245 341f + 805r 805r Stenotrophomonas maltophilia KBP.AS.246 27f + Un1492r 1100r Corynebacterium mucifaciens KBP.AS.247 27f + Un1492r 1100r Bacillus subtilis KBP.AS.248 27f + Un1492r 1100r Corynebacterium mucifaciens KBP.AS.249 27f + Un1492r 1100r Sphingomonas sp. KBP.AS.250 341f + 805r 805r Bacillus sp. KBP.AS.251 27f + Un1492r 1100r Micrococcus sp. KBP.AS.252 27f + Un1492r 1100r Micrococcus sp. KBP.AS.253 27f + Un1492r 1100r Dietzia cinnamea KBP.AS.254 27f + Un1492r 1100r Microbacterium oxydans KBP.AS.255 341f + 805r 805r Micrococcus luteus KBP.AS.256 27f + Un1492r 1100r Microbacterium oxydans KBP.AS.257 Identification was carried out according to phenotypic characters. Streptomyces sp. KBP.AS.258 27f + Un1492r 1100r Arthrobacter crystallopoietes KBP.AS.259 Identification was carried out according to phenotypic characters. Streptomyces sp. KBP.AS.261 27f + Un1492r 1100r Streptomyces sp. KBP.AS.262 27f + 537r 537r Bacillus sp. KBP.AS.265 27f + Un1492r 1100r Pseudomonas frederiksbergensis KBP.AS.267 27f + Un1492r 1100r Glutamicibacter sp. KBP.AS.269 27f + Un1492r 1100r Rhodococcus sp. KBP.AS.270 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.271 27f + Un1492r 1100r Bacillus sp. KBP.AS.272 27f + Un1492r 1100r Bacillus megaterium KBP.AS.275 27f + Un1492r 1100r Microbacterium paraoxydans KBP.AS.282 27f + Un1492r 1100r Pseudomonas sp. KBP.AS.283 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.284 341f + 805r 805r Pseudarthrobacter scleromae KBP.AS.285 27f + Un1492r 1100r Brevibacterium epidermidis KBP.AS.389 27f + Un1492r 1100r Bacillus simplex KBP.AS.390 27f + Un1492r 1100r Rhodococcus sp.
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KBP.AS.391 341f + 805r 805r Klebsiella sp. KBP.AS.392 27f + Un1492r 1100r Microbacterium sp. KBP.AS.393 27f + Un1492r 1100r Arthrobacter sulfonivorans KBP.AS.394 27f + Un1492r 1100r Microbacterium flavescens KBP.AS.395 27f + Un1492r 1100r Acinetobacter pittii KBP.AS.396 27f + 537r 537r Bacillus sp. KBP.AS.397 27f + Un1492r 1100r Microbacterium oxydans KBP.AS.398 27f + Un1492r 1100r Stenotrophomonas rhizophila KBP.AS.399 341f + 805r 805r Microbacterium sp. KBP.AS.400 341f + 805r 805r Pseudomonas putida KBP.AS.401 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.402 27f + Un1492r 1100r Stenotrophomonas maltophilia KBP.AS.403 27f + 537r 537r Rhodococcus sp. KBP.AS.404 27f + Un1492r 1100r Micrococcus sp. KBP.AS.413 27f + Un1492r 1100r Kocuria rosea KBP.AS.414 341f + 805r 805r Bacillus sp. KBP.AS.417 27f + 537r 537r Pseudomonas frederiksbergensis KBP.AS.471 27f + Un1492r 1100r Microbacterium pumilum KBP.AS.472 27f + Un1492r 1100r Microbacterium pumilum KBP.AS.473 27f + Un1492r 1100r Bacillus sp. KBP.AS.474 341f + 805r 805r Pseudomonas frederiksbergensis KBP.AS.475 27f + Un1492r 1100r Bacillus simplex KBP.AS.476 Identification was carried out according to phenotypic characters. Pseudomonas sp. KBP.AS.477 341f + 805r 805r Micrococcus sp. KBP.AS.478 27f + Un1492r 1100r Brachybacterium sp. KBP.AS.479 341f + 805r 805r Brevibacterium aureum KBP.AS.480 27f + Un1492r 1100r Brevibacterium luteolum KBP.AS.481 27f + Un1492r 1100r Bacillus sp. KBP.AS.482 341f + 805r 805r Bacillus sp. KBP.AS.483 27f + Un1492r 1100r Brevibacillus brevis KBP.AS.484 27f + Un1492r 1100r Bacillus sp. KBP.AS.485 27f + Un1492r 1100r Bacillus sp. KBP.AS.486 341f + 805r 805r Pseudarthrobacter sp. KBP.AS.487 27f + Un1492r 1100r Pseudarthrobacter sp. KBP.AS.488 27f + Un1492r 1100r Leucobacter aridicollis KBP.AS.489 341f + 805r 805r Pseudochrobactrum sp. KBP.AS.490 27f + 537r 537r Pseudomonas sp. KBP.AS.491 27f + Un1492r 1100r Kocuria rosea KBP.AS.492 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.493 27f + Un1492r 1100r Ochrobactrum thiophenivorans KBP.AS.494 27f + Un1492r 1100r Agrococcus jenensis KBP.AS.495 27f + Un1492r 1100r Dietzia cinnamea KBP.AS.496 27f + Un1492r 1100r Leucobacter aridicollis KBP.AS.497 Identification was carried out according to phenotypic characters. Micrococcus sp. KBP.AS.498 Identification was carried out according to phenotypic characters. Bacillus sp. KBP.AS.499 27f + Un1492r 1100r Paracoccus marcusii KBP.AS.500 27f + 537r 537r Staphylococcus epidermidis KBP.AS.501 341f + 805r 805r Bacillus sp. KBP.AS.502 27f + 537r 537r Staphylococcus epidermidis KBP.AS.503 27f + Un1492r 1100r Micrococcus terreus KBP.AS.505 27f + 537r 537r Dietzia cinnamea KBP.AS.506 27f + Un1492r 1100r Methylobacterium sp. KBP.AS.511 Identification was carried out according to phenotypic characters. Arthrobacter sp. KBP.AS.513 27f + Un1492r 1100r Leucobacter sp. KBP.AS.514 27f + Un1492r 1100r Brevibacterium sp. KBP.AS.516 341f + 805r 805r Pseudomonas sp. KBP.AS.519 27f + Un1492r 1100r Chryseobacterium kwangjuense KBP.AS.521 341f + 805r 805r Streptomyces sp. KBP.AS.522 341f + 805r 805r Streptomyces sp. KBP.AS.523 27f + Un1492r 1100r Microbacterium oxydans KBP.AS.525 27f + Un1492r 1100r Pseudarthrobacter siccitolerans KBP.AS.526 341f + 805r 805r Pseudomonas oryzihabitans KBP.AS.721 27f + Un1492r 1100r Planomicrobium sp. KBP.AS.722 27f + Un1492r 1100r Planomicrobium okeanokoites KBP.AS.723 27f + Un1492r 1100r Tsukamurella tyrosinosolvens KBP.AS.881 27f + Un1492r 1100r Arthrobacter sp.
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KBP.AS.882 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.885 341f + 805r 805r Pseudomonas sp. KBP.AS.886 341f + 805r 805r Pseudomonas sp. KBP.AS.887 27f + Un1492r 1100r Arthrobacter ginsengisoli KBP.AS.894 27f + Un1492r 1100r Arthrobacter ginsengisoli
Table S2. Taxonomical affiliation of isolated bacteria.
Strain— Sample‐Medium‐ GenBank Cultivation BLAST Search Results Taxonomic Affiliation Accession Temperature Number Bacillus subtilis [CP025941] 97.99% KBP.AS.110 Ice‐PYG‐25 °C Bacillus subtilis [CP002905] 97.99% Bacillus sp. MN093986 Bacillus subtilis [MK696406] 97.88% Bacillus pumilus [CP011007] 99.57% KBP.AS.112 Bacillus safensis [KT274778] 99.46% Ice‐PYG‐25 °C Bacillus sp. MN093987 Bacillus pumilus [MK696261] 99.46% Bacillus safensis [MK696252] 99.46% Ochrobactrum sp. [MG835335] 99.89% KBP.AS.113 Ice‐PYG‐25 °C Ochrobactrum sp. [MF754148] 99.89% Ochrobactrum sp. MN093988 Ochrobactrum sp. [CP022604] 99.89% Arthrobacter ginsengisoli [MH667851] 99.79% KBP.AS.122 Moraine & Ice‐PYG‐ Arthrobacter sp. [MH714681] 99.79% Arthrobacter ginsengisoli MN093989 25 °C Arthrobacter ginsengisoli [MH482302] 99.79% Stenotrophomonas sp. [MH498431] 97.20% Moraine & Ice‐PYG‐ KBP.AS.220 Stenotrophomonas maltophilia [MF381040] 96.88% Stenotrophomonas sp. 25 °C Stenotrophomonas sp. [CP037883] 96.88% Micrococcus sp. [MH734556] 100.00% KBP.AS.230 Moraine & Ice‐PYG‐ Uncultured Micrococcus sp. [MG052577] 99.75% Micrococcus sp. MN093990 25 °C Micrococcus sp. [JX239759] 99.49% Bacillus pumilus [MK696261] 100.00% KBP.AS.231 Moraine & Ice‐PYG‐ Bacillus pumilus [MK696249] 100.00% Bacillus pumilus MN093991 25 °C Bacillus pumilus [MK696242] 100% Micrococcus endophyticus [MK578827] 100.00% KBP.AS.232 Moraine & Ice‐PYG‐ Micrococcus luteus [MH778045] 100.00% Micrococcus sp. MN093992 25°C Micrococcus sp. [MH707206] 100.00% Identification was carried out according to phenotypic KBP.AS.233 Ice‐PYG‐25 °C Rhodococcus sp. characters Bacillus infantis [MK571885] 99.52% KBP.AS.234 Ice‐PYG‐25 °C Bacillus infantis [MK241868] 99.52% Bacillus infantis MN093993 Bacillus infantis [MG892784] 99.52% Identification was carried out according to phenotypic KBP.AS.235 Ice‐PYG‐25 °C Streptomyces sp. characters Arthrobacter sp. [MH714643] 100.00% KBP.AS.236 Moraine & Ice‐PYG‐ Arthrobacter sp. [MG757950] 100.00% Arthrobacter agilis MN093995 25 °C Arthrobacter agilis [LT853743] 100.00% Paenibacillaceae bacterium strain [MG799443] 99.90% KBP.AS.237 Ice‐PYG‐25 °C Paracoccus marcusii [MH744726] 99.79% Paracoccus marcusii MN093995 Paracoccus marcusii [MH725409] 99.79% Brevibacterium sp. [MF381041] 100% KBP.AS.238 Ice‐PYG‐25 °C Brevibacterium sp. [KT799843] 99.65% Brevibacterium linens MN093996 Brevibacterium linens [KY307903] 99.54%
KBP.AS.239 Ice‐PYG‐25 °C Micrococcus sp. [MH707205] 99.50% Micrococcus terreus
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MN093997 Micrococcus terreus [MG271958] 99.50% Micrococcus sp. [MF041849] 99.50% Brevibacterium sp. [KT799843] 99.42% KBP.AS.240 Ice‐PYG‐25 °C Brevibacterium oceani [MF769004] 99.05% Brevibacterium sp. MN093998 Brevibacterium linens [KY307903] 98.85% Streptomyces sp. [MK696315] 99.50% KBP.AS.241 Ice‐PYG‐25 °C Streptomyces sp. [MH311922] 99.50% Streptomyces sp. MN093999 Streptomyces sp. [MH311919] 99.50% Microbacterium maritypicum [KJ004614] 99.67% KBP.AS.242 Ice‐PYG‐25 °C Microbacterium oxydans [MK696389] 99.57% Microbacterium sp. MN094000 Microbacterium sp. [CP024170] 99.57% Pseudomonas sp. [LC420204] 99.52% KBP.AS.244 Ice‐PYG‐25 °C Pseudomonas sp. [LC420202] 99.52% Pseudomonas sp. MN094001 Pseudomonas sp. [LC420201] 99.52% Stenotrophomonas sp. [CP037883] 100.00% KBP.AS.245 Ice‐PYG‐25 °C Stenotrophomonas maltophilia [MK641667] 100.00% Stenotrophomonas maltophilia MN094002 Stenotrophomonas maltophilia [MK641664] 100.00% Corynebacterium mucifaciens [MH715174] 99.88% KBP.AS.246 Ice‐PYG‐25 °C Corynebacterium mucifaciens [HQ337895] 99.88% Corynebacterium mucifaciens MN094003 Uncultured bacterium clone [KF065559] 99.88% Bacillus subtilis [MK696406] 99.88% KBP.AS.247 Ice‐PYG‐25°C Bacillus subtilis [KY652944] 99.88% Bacillus subtilis MN094004 Bacillus subtilis [KY652934] 99.88% Corynebacterium mucifaciens [MH715174] 96.94% KBP.AS.248 Ice‐PYG‐25 °C Corynebacterium mucifaciens [KU922487] 96.94% Corynebacterium mucifaciens MN094005 Corynebacterium mucifaciens [KT720152] 96.94% Sphingomonas mucosissima [KX966415] 99.27% KBP.AS.249 Ice‐PYG‐25 °C Sphingomonas sp. [KP710195] 99.27% Sphingomonas sp. MN094006 Sphingomonas olei [NR_157757] 99.17% Bacillus zhangzhouensis [MK712418] 100.00% KBP.AS.250 Moraine & Ice‐PYG‐ Bacillus pumilus [MK696261] 100.00% Bacillus sp. MN094007 25 °C Bacillus safensis [MK696252] 100.00% Micrococcus endophyticus [MK578827] 100.00% KBP.AS.251 Moraine‐PYG‐25 °C Micrococcus luteus [MH778045] 100.00% Micrococcus sp. MN094008 Micrococcus luteus [MG594812] 100.00% Micrococcus sp. [MG979737] 99.79% KBP.AS.252 Moraine‐PYG‐25 °C Micrococcus sp. [MK605971] 99.79% Micrococcus sp. MN094009 Micrococcus sp. [MK578271] 99.79% Dietzia sp. [CP027238] 99.27% KBP.AS.253 Moraine‐PYG‐25 °C Dietzia cinnamea [NR_042390] 99.27% Dietzia cinnamea MN094010 Dietzia cinnamea [LT160785] 99.17% Microbacterium oxydans [MK696389] 100.00% KBP.AS.254 Moraine‐PYG‐25 °C Microbacterium sp. [CP024170] 100.00% Microbacterium oxydans MN094011 Microbacterium oxydans [KM894196] 100.00% Uncultured Micrococcus sp. [MG052577] 99.75% KBP.AS.255 Moraine‐PYG‐25 °C Micrococcus luteus [MG997087] 99.50% Micrococcus luteus MN094012 Micrococcus luteus [MK629834] 99.50% Microbacterium oxydans [MK696389] 99.68% KBP.AS.256 Moraine‐PYG‐25 °C Microbacterium sp. [CP024170] 99.68% Microbacterium oxydans MN094013 Microbacterium oxydans [MF554625] 99.68% Identification was carried out according to phenotypic KBP.AS.257 Moraine‐CM‐25 °C Streptomyces sp. characters KBP.AS.258 Arthrobacter crystallopoietes [KC778371] 99.76% Moraine‐CM‐25 °C Arthrobacter crystallopoietes MN094014 Arthrobacter crystallopoietes [MH200640] 99.64%
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Arthrobacter crystallopoietes [CP018864] 99.64%
Identification was carried out according to phenotypic KBP.AS.259 Moraine‐CM‐25 °C Streptomyces sp. characters Streptomyces sp. [MK134629] 99.60% KBP.AS.261 Moraine‐CM‐25 °C Streptomyces sp. [MK134627] 99.60% Streptomyces sp. MN094015 Streptomyces sp. [MH299489] 99.60% Bacillus zhangzhouensis [MK712418] 98.45% KBP.AS.262 Moraine‐CM‐25 °C Bacillus zhangzhouensis [MK696265] 98.45% Bacillus sp. Bacillus pumilus [MK696262] 98.45% Pseudomonas frederiksbergensis [MH421846] 99.58% KBP.AS.265 Pseudomonas Moraine‐CM‐25 °C Pseudomonas frederiksbergensis [MF044058] 99.58% MN094016 frederiksbergensis Pseudomonas sp. [KY476609] 99.58% KBP.AS.267 Glutamicibacter sp. [MG646025] 99.47% Glutamicibacter arilaitensis Moraine‐CM‐25 °C Glutamicibacter sp. MN094017 [MG788347] 99.47% Glutamicibacter nicotianae [KX698104] 99.47% Rhodococcus sovatensis [MK696258] 99.82% KBP.AS.269 Ice‐CM‐25 °C Rhodococcus fascians [MK178494] 99.82% Rhodococcus sp. MN094018 Rhodococcus fascians [MH917340] 99.82% Stenotrophomonas maltophilia [MK641667] 99.88% KBP.AS.270 Ice‐CM‐25 °C Stenotrophomonas maltophilia [MK641655] 99.88% Stenotrophomonas maltophilia MN094019 Stenotrophomonas maltophilia [MH379727] 99.88% Bacillus safensis [MK640707] 100.00% KBP.AS.271 Ice‐CM‐25 °C Bacillus safensis [FN666615] 100.00% Bacillus sp. MN094020 Bacillus pumilus [FN652913] 100.00% Bacillus megaterium [MK629317] 100.00% KBP.AS.272 Ice‐CM‐25 °C Bacillus megaterium [MK618596] 100.00% Bacillus megaterium MN094021 Bacillus megaterium [MK087747] 100.00% Microbacterium paraoxydans [MK246150] 100.00% KBP.AS.275 Ice‐CM‐25 °C Microbacterium paraoxydans [MH819720] 100.00% Microbacterium paraoxydans MN094022 Microbacterium paraoxydans [MK106301] 100.00% Pseudomonas aeruginosa [MG996796] 98.07% KBP.AS.282 Moraine & Ice‐CM‐ Pseudomonas oryzihabitans [MH464584] 98.07% Pseudomonas sp. MN094023 25 °C Pseudomonas psychrotolerans [KY882118] 98.07% Arthrobacter ginsengisoli [MH667851] 99.41% KBP.AS.283 Moraine & Ice‐CM‐ Arthrobacter ginsengisoli [MH667849] 99.41% Arthrobacter ginsengisoli MN094024 25 °C Arthrobacter ginsengisoli [MH482302] 99.41% Pseudarthrobacter scleromae [LT978388] 99.41% KBP.AS.284 Moraine & Ice‐CM‐ Pseudarthrobacter scleromae [LT978369] 99.41% Pseudarthrobacter scleromae MN094025 25 °C Pseudarthrobacter scleromae [LT978366] 99.41% Brevibacterium epidermidis [KY928098] 99.44% KBP.AS.285 Moraine & Ice‐CM‐ Brevibacterium epidermidis [KP318464] 99.33% Brevibacterium epidermidis MN094026 25 °C Brevibacterium epidermidis [EU046495] 99.33% Bacillus simplex [MK484393] 99.89% KBP.AS.389 Ice‐PYG‐10 °C Bacillus simplex [MK484392] 99.89% Bacillus simplex MN094027 Bacillus simplex [MK484391] 99.89% Rhodococcus fascians [MK178494] 100.00% KBP.AS.390 Ice‐PYG‐10 °C Rhodococcus fascians [MH917340] 100.00% Rhodococcus sp. MN094028 Rhodococcus cerastii [MG645192] 100.00% Uncultured Klebsiella sp. [KT300809] 97.13% KBP.AS.391 Ice‐PYG‐10 °C Uncultured Klebsiella sp. [KT297703] 96.79% Klebsiella sp. MN094029 Uncultured Klebsiella sp. [KT297462] 96.79% Microbacterium sp. [MH714654] 99.39% KBP.AS.392 Ice‐PYG‐10 °C Microbacterium sp. [MH298697] 99.39% Microbacterium sp. MN094030 Microbacterium sp. [KX784539] 99.39%
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Arthrobacter sulfonivorans [KC462926] 99.77% KBP.AS.393 Ice‐PYG‐10 °C Arthrobacter sulfonivorans [KF537271] 99.77% Arthrobacter sulfonivorans MN094031 Arthrobacter sulfonivorans [KF537269] 99.77% Microbacterium sp. [MK356413] 99.79% KBP.AS.394 Ice‐PYG‐10 °C Microbacterium flavescens [JF778699] 99.48% Microbacterium flavescens MN094032 Microbacterium flavescens [KJ726613] 99.27% Acinetobacter pittii [CP033535] 99.68% KBP.AS.395 Ice‐PYG‐10 °C Acinetobacter pittii [MH144322] 99.68% Acinetobacter pittii MN094033 Acinetobacter pittii [MH144316] 99.68% Bacillus safensis [MK680802] 100.00% KBP.AS.396 Ice‐PYG‐10 °C Bacillus safensis [MK577399] 100.00% Bacillus sp. MN094034 Bacillus safensis [MK285605] 100.00% Microbacterium oxydans [CP031338] 99.70% KBP.AS.397 Ice‐PYG‐10 °C Microbacterium oxydans [CP031422] 99.70% Microbacterium oxydans MN094035 Microbacterium oxydans [MF459692] 99.70% Stenotrophomonas rhizophila [MK318581] 100.00% KBP.AS.398 Moraine & Ice‐PYG‐ Stenotrophomonas rhizophila [KT204469] 100.00% Stenotrophomonas rhizophila MN094036 10 °C Stenotrophomonas rhizophila [EU977698] 100.00% Microbacterium oxydans [MK696389] 100.00% KBP.AS.399 Moraine & Ice‐PYG‐ Microbacterium foliorum [MK696388] 100.00% Microbacterium sp. MN094037 10 °C Microbacterium testaceum [MK629778] 100.00% Pseudomonas putida [MH734593] 100.00% KBP.AS.400 Moraine & Ice‐PYG‐ Pseudomonas putida [MG846038] 99.76% Pseudomonas putida MN094038 10°C Pseudomonas putida [MK712480] 99.52% Stenotrophomonas maltophilia [KM114934] 99.79% KBP.AS.401 Moraine & Ice‐PYG‐ Stenotrophomonas maltophilia [MK641667] 99.68% Stenotrophomonas maltophilia MN094039 10 °C Stenotrophomonas maltophilia [MK641655] 99.68% Stenotrophomonas maltophilia [MK641667] 100.00% KBP.AS.402 Moraine & Ice‐PYG‐ Stenotrophomonas maltophilia [MK641655] 100.00% Stenotrophomonas maltophilia MN094040 10 °C Stenotrophomonas maltophilia [MH379727] 100.00% Rhodococcus sp. [FN392689] 100.00% KBP.AS.403 Moraine‐PYG‐10 °C Rhodococcus fascians [FR851921] 100.00% Rhodococcus sp. MN094041 Rhodococcus cerastii [MK224797] 100.00% Micrococcus sp. [MK605971] 99.69% KBP.AS.404 Moraine‐PYG‐10 °C Micrococcus luteus [MG996877] 99.69% Micrococcus sp. MN094042 Micrococcus yunnanensis [MG996834] 99.69% Kocuria rosea [MK696247] 99.89% KBP.AS.413 Ice‐PYG‐10 °C Kocuria rosea [MK696235] 99.89% Kocuria rosea MN094043 Kocuria rosea [MK100469] 99.89% Bacillus safensis [CP032830] 100.00% KBP.AS.414 Moraine‐PYG‐10 °C Bacillus safensis [MK499471] 100.00% Bacillus sp. MN094044 Bacillus pumilus [MK371093] 100.00% Pseudomonas frederiksbergensis [HQ588832] 99.79% KBP.AS.417 Moraine‐PYG‐25 °C Pseudomonas mandelii [FM955880] 99.79% Pseudomonas frederiksbergensis MN094045 Pseudomonas frederiksbergensis [NR_028906] 99.79% Microbacterium pumilum [LN623621] 99.89% KBP.AS.471 Moraine‐PYG‐25 °C Microbacterium pumilum [LK391536] 99.89% Microbacterium pumilum MN094046 Microbacterium pumilum [KC213957] 99.89% Microbacterium pumilum [LK391536] 99.69% KBP.AS.472 Moraine‐PYG‐25 °C Microbacterium pumilum [KC213957] 99.49% Microbacterium pumilum MN094047 Microbacterium pumilum [LN623621] 99.49% Bacillus cereus [HF570102] 98.97% KBP.AS.473 Moraine‐PYG‐25 °C Bacillus cereus [HE660037] 98.85% Bacillus sp. MN094048 Bacillus thuringiensis [CP021436] 98.85%
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Pseudomonas frederiksbergensis [MF044058] 100.00% KBP.AS.474 Pseudomonas Moraine‐PYG‐25 °C Pseudomonas frederiksbergensis [KT695824] 100.00% MN094049 frederiksbergensis Pseudomonas frederiksbergensis [MH144323] 100.00% Bacillus sp. [KU236475] 98.92% KBP.AS.475 Moraine‐PYG‐25 °C Bacillus simplex [MK484393] 98.80% Bacillus simplex MN094050 Bacillus simplex [MK484392] 98.80% Identification was carried out according to phenotypic KBP.AS.476 Moraine‐PYG‐25 °C Pseudomonas sp. characters Micrococcus luteus [MK629834] 99.73% KBP.AS.477 Moraine‐PYG‐25 °C Micrococcus antarcticus [MK629795] 99.73% Micrococcus sp. MN094051 Micrococcus endophyticus [MK615927] 99.73% Brachybacterium sp. [KU560389] 98.48% KBP.AS.478 Moraine‐PYG‐25 °C Brachybacterium sp. [DQ868687] 98.48% Brachybacterium sp. MN094052 Brachybacterium paraconglomeratum [MN004835] 98.31% Brevibacterium aureum [MF526523] 100.00% KBP.AS.479 Moraine‐PYG‐25 °C Brevibacterium aureum [MF525575] 100.00% Brevibacterium aureum MN094053 Brevibacterium aureum [MF525396] 100.00% Brevibacterium luteolum [MK205175] 99.78% KBP.AS.480 Moraine‐PYG‐25 °C Brevibacterium luteolum [KX981422] 99.78% Brevibacterium luteolum MN094054 Brevibacterium luteolum [KT722610] 99.78% Bacillus safensis [MK640707] 99.89% KBP.AS.481 Moraine‐PYG‐25 °C Bacillus safensis [FN666615] 99.89% Bacillus sp. MN094055 Bacillus pumilus [FN652913] 99.89% Uncultured bacterium [LT720430] 99.76% KBP.AS.482 Moraine‐PYG‐25 °C Bacillus zhangzhouensis [MK712418] 99.52% Bacillus sp. MN094056 Bacillus zhangzhouensis [MK696265] 99.52% Brevibacillus brevis [LR134338] 99.78% KBP.AS.483 Moraine‐PYG‐25 °C Brevibacillus brevis [CP030117] 99.78% Brevibacillus brevis MN094057 Brevibacillus brevis [MG986218] 99.78% Bacillus safensis [MK640707] 100.00% KBP.AS.484 Moraine & Ice‐PYG‐ Bacillus safensis [FN666615] 100.00% Bacillus sp. MN094058 25 °C Bacillus pumilus [FN652913] 100.00% Bacillus pumilus [MK696261] 99.89% KBP.AS.485 Moraine & Ice‐PYG‐ Bacillus safensis [MK696252] 99.89% Bacillus sp. MN094059 25 °C Bacillus pumilus [MK696249] 99.89% Pseudarthrobacter siccitolerans [MK696248] 100.00% KBP.AS.486 Moraine & Ice‐PYG‐ Pseudarthrobacter scleromae [LT978388] 100.00% Pseudarthrobacter sp. MN094060 25 °C Pseudarthrobacter scleromae [LT978369] 100.00% Pseudarthrobacter scleromae [KY419841] 99.58% KBP.AS.487 Moraine & Ice‐PYG‐ Pseudarthrobacter oxydans [KU995318] 99.58% Pseudarthrobacter sp. MN094061 25 °C Pseudarthrobacter sp. [MH698784] 99.48% Leucobacter aridicollis [KJ742507] 99.69% KBP.AS.488 Moraine & Ice‐PYG‐ Leucobacter aridicollis [KM874408] 99.69% Leucobacter aridicollis MN094062 25 °C Leucobacter aridicollis [KM874407] 99.69% Pseudochrobactrum sp. [MK583624] 99.45% KBP.AS.489 Moraine & Ice‐PYG‐ Pseudochrobactrum sp. [MK095773] 99.45% Pseudochrobactrum sp. MN094063 25 °C Pseudochrobactrum sp. [MK095772] 99.45% Pseudomonas sp. [AY263473] 98.91% KBP.AS.490 Moraine & Ice‐PYG‐ Pseudomonas sp. [MK140954] 98.70% Pseudomonas sp. MN094064 25 °C Pseudomonas sp. [MK371088] 98.70% Kocuria rosea [MK696247] 98.83% KBP.AS.491 Moraine & Ice‐PYG‐ Kocuria rosea [MK696235] 98.83% Kocuria rosea MN094065 25 °C Kocuria rosea [MK648258] 98.83%
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Arthrobacter sp. [JF313077] 99.89% KBP.AS.492 Moraine & Ice‐PYG‐ Arthrobacter ginsengisoli [MH667851] 99.78% Arthrobacter ginsengisoli MN094066 25 °C Arthrobacter ginsengisoli [MH667849] 99.78% Ochrobactrum thiophenivorans [KY819002] 100.00% KBP.AS.493 Moraine & Ice‐PYG‐ Ochrobactrum thiophenivorans [KY178282] 100.00% Ochrobactrum thiophenivorans MN094067 25 °C Ochrobactrum thiophenivorans [KU949758] 100.00% Agrococcus jenensis [LT853740] 99.89% KBP.AS.494 Ice‐PYG‐25 °C Agrococcus jenensis [MG661754] 99.68% Agrococcus jenensis MN094068 Agrococcus jenensis [JX841074] 99.57% Dietzia sp. [CP027238] 99.89% KBP.AS.495 Ice‐PYG‐25 °C Dietzia cinnamea [NR_042390] 99.89% Dietzia cinnamea MN094069 Dietzia cinnamea [LT160785] 99.78% Leucobacter aridicollis [KR827428] 99.89% KBP.AS.496 Ice‐PYG‐25 °C Leucobacter aridicollis [KM874408] 99.89% Leucobacter aridicollis MN094070 Leucobacter aridicollis [KM874407] 99.89% Identification was carried out according to phenotypic KBP.AS.497 Ice‐PYG‐25 °C Micrococcus sp. characters Identification was carried out according to phenotypic KBP.AS.498 Ice‐PYG‐25 °C Bacillus sp. characters Paracoccus marcusii [MK106317] 99.46% KBP.AS.499 Ice‐PYG‐25 °C Paracoccus marcusii [MH744726] 99.46% Paracoccus marcusii MN094071 Paracoccus marcusii [MH725412] 99.46% Staphylococcus epidermidis [CP034115] 100.00% KBP.AS.500 Ice‐PYG‐25 °C Staphylococcus epidermidis [CP034111] 100.00% Staphylococcus epidermidis MN094072 Staphylococcus epidermidis [CP033782] 100.00% Bacillus zhangzhouensis [MK712418] 100.00% KBP.AS.501 Ice‐PYG‐25 °C Bacillus zhangzhouensis [MK696265] 100.00% Bacillus sp. MN094073 Bacillus pumilus [MK696261] 100.00% Staphylococcus epidermidis [CP034115] 100.00% KBP.AS.502 Ice‐PYG‐25 °C Staphylococcus epidermidis [CP034111] 100.00% Staphylococcus epidermidis MN094074 Staphylococcus epidermidis [CP033782] 100.00% Micrococcus terreus [KU921566] 100.00% KBP.AS.503 Ice‐PYG‐25 °C Micrococcus terreus [MH174421] 100.00% Micrococcus terreus MN094075 Micrococcus terreus [MG271958] 100.00% Uncultured Dietziaceae bacterium clone [MG802689] 99.21% KBP.AS.505 Ice‐PYG‐25 °C Dietzia cinnamea [LT935684] 97.64% Dietzia cinnamea Dietzia cinnamea [LT935681] 97.64% Methylobacterium extorquens [MG280711] 99.37% KBP.AS.506 Moraine‐PYG‐25 °C Methylobacterium extorquens [KF572999] 99.37% Methylobacterium sp. MN094076 Methylobacterium zatmanii [AB698688] 99.37% Moraine & Ice‐CM‐ Identification was carried out according to phenotypic KBP.AS.511 Arthrobacter sp. 10 °C characters Leucobacter aridicollis [KJ742507] 98.07% KBP.AS.513 Moraine & Ice‐CM‐ Leucobacter aridicollis [MK403801] 98.00% Leucobacter sp. MN094077 10 °C Leucobacter aridicollis [KR827428] 98.00% Brevibacterium linens [KY307903] 99.75% KBP.AS.514 Moraine & Ice‐CM‐ Brevibacterium avium [KF876886] 99.75% Brevibacterium sp. MN094078 10 °C Brevibacterium permense [LN651152] 99.62% Pseudomonas orientalis [CP027726] 100.00% KBP.AS.516 Moraine & Ice‐CM‐ Pseudomonas marginalis [MK123855] 100.00% Pseudomonas sp. MN094079 10 °C Pseudomonas marginalis [MK123854] 100.00% Chryseobacterium kwangjuense [MK070021] 99.78% KBP.AS.519 Moraine & Ice‐CM‐ Chryseobacterium kwangjuense [NR_108175] 99.78% Chryseobacterium kwangjuense MN094080 10 °C Chryseobacterium kwangjuense [KU712021] 99.12%
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Streptomyces sp. [MK696315] 99.49% KBP.AS.521 Moraine‐CM‐10 °C Streptomyces sp. [MH311922] 99.49% Streptomyces sp. MN094081 Streptomyces badius [MK680817] 99.49% Streptomyces sp. [MK696315] 99.48% KBP.AS.522 Moraine‐CM‐10 °C Streptomyces sp. [MH311922] 99.48% Streptomyces sp. MN094082 Streptomyces sp. [MH311919] 99.48% Microbacterium oxydans [MK696389] 99.58% KBP.AS.523 Moraine‐CM‐10 °C Microbacterium oxydans [MF554625] 99.58% Microbacterium oxydans MN094083 Microbacterium oxydans [KM894196] 99.58% Pseudarthrobacter siccitolerans [MH371336] 100.00% KBP.AS.525 Moraine‐CM‐10 °C Pseudarthrobacter siccitolerans [MF682021] 100.00% Pseudarthrobacter siccitolerans MN094084 Pseudarthrobacter siccitolerans [MF681903] 100.00% Pseudomonas oryzihabitans [MH464584] 99.52% KBP.AS.526 Moraine‐CM‐10 °C Pseudomonas sp. [MH319988] 99.52% Pseudomonas oryzihabitans MN094085 Pseudomonas oryzihabitans [LC389519] 99.52% Planomicrobium sp. [KP324949] 99.14% KBP.AS.721 Ice‐PYG‐25 °C Planomicrobium sp. [MF041830] 98.70% Planomicrobium sp. MN094086 Planomicrobium sp. [JX949540] 98.59% Planococcaceae bacterium [KF600276] 100.00% KBP.AS.722 Ice‐PYG‐25 °C Planomicrobium okeanokoites [KF749394] 99.87% Planomicrobium okeanokoites MN094087 Planomicrobium okeanokoites [KF749386] 99.87% Tsukamurella tyrosinosolvens [LR134452] 99.35% KBP.AS.723 Ice‐PYG‐25 °C Tsukamurella tyrosinosolvens [LR134450] 99.35% Tsukamurella tyrosinosolvens MN094088 Tsukamurella tyrosinosolvens [MH393216] 99.35% Arthrobacter sp. [JF313077] 99.88% KBP.AS.881 Moraine‐PYG‐25 °C Arthrobacter sp. [MK558843] 99.77% Arthrobacter sp. MN094089 Arthrobacter sp. [MK558838] 99.77% Arthrobacter ginsengisoli [MH667851] 99.88% KBP.AS.882 Moraine‐PYG‐25 °C Arthrobacter ginsengisoli [MH667849] 99.88% Arthrobacter ginsengisoli MN094090 Arthrobacter ginsengisoli [MH482302]99.88% Pseudomonas prosekii [MH930044] 99.52% KBP.AS.885 Moraine‐PYG‐25 °C Pseudomonas prosekii [MH929959] 99.52% Pseudomonas sp. MN094091 Pseudomonas mandelii [MH929549] 99.52% Pseudomonas prosekii [MH930044] 98.81% KBP.AS.886 Ice‐PYG‐25 °C Pseudomonas prosekii [MH929959] 98.81% Pseudomonas sp. MN094092 Pseudomonas mandelii [MH929481] 98.81% Arthrobacter sp. [JF313077] 100.00% KBP.AS.887 Moraine & Ice‐PYG‐ Arthrobacter ginsengisoli [MH667851] 99.88% Arthrobacter ginsengisoli MN094093 25 °C Arthrobacter ginsengisoli [MH667849] 99.88% Arthrobacter ginsengisoli [MH667851] 100.00% KBP.AS.894 Moraine‐PYG‐25 °C Arthrobacter ginsengisoli [MH667849] 100.00% Arthrobacter ginsengisoli MN094094 Arthrobacter ginsengisoli [MH482302] 100.00% Ochrobactrum thiophenivorans [KY819002] 99.88% KBP.AS.1261 Ice‐CM‐10 °C Ochrobactrum thiophenivorans [KY178282] 99.88% Ochrobactrum thiophenivorans MN094095 Ochrobactrum thiophenivorans [KU949758] 99.88% Arthrobacter ginsengisoli [MH667851] 99.28% KBP.AS.1262 Ice‐CM‐10 °C Arthrobacter ginsengisoli [MH667849] 99.28% Arthrobacter ginsengisoli MN094096 Arthrobacter ginsengisoli [MH482302] 99.28% Identification was carried out according to phenotypic KBP.AS.1263 Ice‐CM‐10 °C Streptomyces sp. characters Ochrobactrum thiophenivorans [KY819002] 100.00% KBP.AS.1264 Ice‐CM‐10 °C Ochrobactrum thiophenivorans [KY178282] 100.00% Ochrobactrum thiophenivorans MN094097 Ochrobactrum thiophenivorans [KU949758] 100.00%
KBP.AS.1265 Ice‐CM‐10 °C Stenotrophomonas maltophilia [MK641667] 99.28% Stenotrophomonas maltophilia
Geosciences 2020, 10, 67 11 of 25
MN094098 Stenotrophomonas maltophilia [MK641655] 99.28% Stenotrophomonas maltophilia [MK629813] 99.28% Uncultured bacterium clone [EU539648] 99.76% KBP.AS.1266 Ice‐CM‐10 °C Uncultured bacterium clone [FJ675504] 98.58% Paracoccus sp. MN094099 Paracoccus sp. [MF804955] 98.22% Stenotrophomonas maltophilia [MK641667] 99.87% KBP.AS.1267 Ice‐CM‐10 °C Stenotrophomonas maltophilia [MK641655] 99.87% Stenotrophomonas maltophilia MN094100 Stenotrophomonas maltophilia [MK629813] 99.87% Stenotrophomonas maltophilia [MK641667] 99.65% KBP.AS.1268 Ice‐CM‐10 °C Stenotrophomonas maltophilia [MK641655] 99.65% Stenotrophomonas maltophilia MN094101 Stenotrophomonas maltophilia [MH379727] 99.65% Stenotrophomonas sp. [MH512640] 98.25%. KBP.AS.1269 Ice‐CM‐10 °C Stenotrophomonas sp. [MH512638] 98.25% Stenotrophomonas sp. MN094102 Stenotrophomonas lactitubi [MH119687] 98.25% Stenotrophomonas maltophilia [MK641667] 100% KBP.AS.1270 Ice‐CM‐10 °C Stenotrophomonas maltophilia [MK641664] 100.00% Stenotrophomonas maltophilia MN094103 Stenotrophomonas maltophilia [MK641655] 100.00% Enterobacter hormaechei [MK282229] 100.00% KBP.AS.1271 Moraine‐PYG‐25 °C Enterobacter hormaechei [MK282228] 100.00% Enterobacter sp. MN094104 Enterobacter asburiae [AP019533] 100.00% Stenotrophomonas maltophilia [MK641667] 99.74% KBP.AS.1272 Ice‐CM‐10 °C Stenotrophomonas maltophilia [MK641664] 99.74% Stenotrophomonas maltophilia MN094105 Stenotrophomonas maltophilia [MK641655] 99.74% Ochrobactrum thiophenivorans [KY178282] 99.89% KBP.AS.1273 Ice‐CM‐10 °C Ochrobactrum thiophenivorans [KY819002] 99.78% Ochrobactrum thiophenivorans MN094106 Ochrobactrum thiophenivorans [KU949758] 99.78% Microbacterium paraoxydans [MK246150] 99.89% KBP.AS.1275 Ice‐CM‐10 °C Microbacterium paraoxydans [MH819720] 99.89% Microbacterium paraoxydans MN094107 Microbacterium paraoxydans [MK106301] 99.89%
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MH174421 Micrococcus terreus 63 KBP.AS.502 Micrococcus sp. JQ228588 Micrococcus sp. 63 MH707205 Micrococcus sp. KBP.AS.403 Rhodococcus sp. Actinobacteria NR 037021 Rhodococcus fascians 63 NR 117103 Rhodococcus cerastii KBP.AS.505 Dietzia cinnamea MH298652 Dietzia cinnamea NR 116686 Dietzia cinnamea KBP.AS.490 Pseudomonas sp. AJ889841 Pseudomonas syringae KX276592 Pseudomonas silesiensis Proteobacteria 97 KBP.AS.417 Pseudomonas frederiksbergensis NR 028906 Pseudomonas frederiksbergensis MH900188 Staphylococcus epidermidis KBP.AS.500 Staphylococcus epidermidis 99 NR 113405 Staphylococcus saccharolyticus NR 117946 Bacillus amyloliquefaciens Firmicutes KBP.AS.262 Bacillus sp. MK185073 Bacillus zhangzhouensis 89 KBP.AS.396 Bacillus sp. KY990920 Bacillus safensis
0.02
Figure S2. Phylogenetic analysis tree of 16S rRNA genes of the strains isolated from the Novaya Zemlya archipelago samples, which were sequenced using 537r primer. The tree was constructed using the Neighbor‐Joining method with the use of the most closely related sequences from GenBank. The number of taxa—23. The number of significant characters—51. The number of bootstrap replicates—500. Confidence values of branching are given for cases of 50% and higher. Circles indicates the strains, isolated from the moraine sample [Nz 1], rhombus—strains isolated from the moraine and ice sample [Nz 2], triangles—isolated from the ice sample [Nz 3]. The scale under the tree indicates the number of phylogenetical significant changes of nucleotides.
Geosciences 2020, 10, 67 13 of 25
NR 024902 Pseudomonas mandelii Pseudomonas frederiksbergensis KBP.AS.474 Pseudomonas sp. KBP.AS.886 Pseudomonas sp. KBP.AS.885 NR 132724 Pseudomonas prosekii NR 037135 Pseudomonas corrugata NR 028929 Pseudomonas kilonensis 60 NR 117826 Pseudomonas corrugata NR 029042 Pseudomonas lini
6274 Pseudomonas sp. KBP.AS.244 59 NR 117177 Pseudomonas frederiksbergensis 80 NR 152639 Pseudomonas caspiana NR 108461 Pseudomonas asturiensis 97 63 KX186966 Pseudomonas putida 85 Pseudomonas putida KBP.AS.400 Pseudomonas sp. KBP.AS.516 99 KX186936 Pseudomonas antarctica 72 KX186942 Pseudomonas extremaustralis 66 NR 117821 Pseudomonas marginalis Proteobacteria MH281747 Pseudomonas oryzihabitans 54 MH281750 Pseudomonas psychrotolerans 99 Pseudomonas oryzihabitans KBP.AS.526 Klebsiella sp. KBP.AS.391 LT548976 Erwinia aphidicola
99 MF425572 Klebsiella pneumoniae 91 MK603176 Enterobacter hormaechei 74 MF061991 Enterobacter cancerogenus Enterobacter sp. KBP.AS.1271 NR 102493 Klebsiella aerogenes KX274129 Klebsiella pneumoniae
72 Stenotrophomonas sp. KBP.AS.1269 Stenotrophomonas maltophilia KBP.AS.1272 99 LT222224 Stenotrophomonas maltophilia 89 Stenotrophomonas maltophilia KBP.AS.1270 Stenotrophomonas maltophilia KBP.AS.245 NR 042519 Pseudochrobactrum kiredjianiae
99 NR 042473 Pseudochrobactrum saccharolyticum 79 Pseudochrobactrum sp. KBP.AS.489 GQ911554 Bacillus pumilus NR 148786 Bacillus zhangzhouensis Bacillus sp. KBP.AS.501 58 Bacillus sp. KBP.AS.414 Bacillus sp. KBP.AS.250 Bacillus sp. KBP.AS.482 99 Firmicutes KX261622 Bacillus pumilus MK424279 Bacillus safensis 99 NR 148787 Bacillus australimaris Bacillus infantis KBP.AS.234 NR 043267 Bacillus infantis 99 NR 118438 Bacillus drentensis Streptomyces sp. KBP.AS.241 Streptomyces sp. KBP.AS.521 99 Streptomyces sp. KBP.AS.522 KY820858 Streptomyces fulvissimus NR 149222 Streptomyces aridus 76 Brevibacterium aureum KBP.AS.479 99 99 NR 042458 Brevibacterium oceani NR 025727 Brevibacterium celere Microbacterium sp. KBP.AS.399 99 MK424292 Microbacterium phyllosphaerae 94 NR 119270 Microbacterium saperdae NR 041824 Pseudarthrobacter scleromae Actinobacteria 98 60 Pseudarthrobacter scleromae KBP.AS.284 NR 117356 Paeniglutamicibacter cryotolerans 93 Micrococcus terreus KBP.AS.239 91 NR 116649 Micrococcus terreus Micrococcus sp. KBP.AS.230 52 NR 026200 Micrococcus lylae 58 NR 134088 Micrococcus aloeverae KY933306 Micrococcus endophyticus KJ476723 Micrococcus luteus Micrococcus luteus KBP.AS.255 Micrococcus sp. KBP.AS.477
0.02 Geosciences 2020, 10, 67 14 of 25
Figure S3. Phylogenetic analysis tree of 16S rRNA genes of the strains isolated from the Novaya Zemlya archipelago samples, which were sequenced using 805r primer. The tree was constructed using the Neighbor‐Joining method with the use of the most closely related sequences from GenBank. The number of taxa—56. The number of significant characters—275. The number of bootstrap replicates—500. Confidence values of branching are given for cases of 50% and higher. Circles indicates the strains, isolated from the moraine sample [Nz 1], rhombus—strains isolated from the moraine and ice sample [Nz 2], triangles—isolated from the ice sample [Nz 3]. The scale under the tree indicates the number of phylogenetical significant changes of nucleotides.
Geosciences 2020, 10, 67 15 of 25
70 Arthrobacter sulfonivorans KBP.AS.393 Arthrobacter ginsengisoli KBP.AS.122 Arthrobacter ginsengisoli KBP.AS.882 Arthrobacter sp. KBP.AS.881 KF212463 Arthrobacter ginsengisoli 65 Pseudarthrobacter sp. KBP.AS.487 Arthrobacter ginsengisoli KBP.AS.492 Arthrobacter ginsengisoli KBP.AS.887 KU995318 Pseudarthrobacter oxydans Arthrobacter ginsengisoli KBP.AS.1262
93 KU512892 Pseudarthrobacter sulfonivorans 51 MH698784 Pseudarthrobacter sp. 65 Pseudarthrobacter siccitolerans KBP.AS.525
99 60 NR 108849 Pseudarthrobacter siccitolerans Arthrobacter ginsengisoli KBP.AS.894 92 Arthrobacter ginsengisoli KBP.AS.283 NR 042251 Arthrobacter tecti
96 Arthrobacter agilis KBP.AS.236 100 LT853743 Arthrobacter agilis 85 53 MK424284 Glutamicibacter halophytocola 100 NR 044338 Glutamicibacter soli Glutamicibacter sp. KBP.AS.267 73 MK696247 Kocuria rosea 100 MN006548 Kocuria rosea 85 99 Kocuria rosea KBP.AS.413 Arthrobacter crystallopoietes KBP.AS.258 53 MK720402 Arthrobacter crystallopoietes 61 74 NR 043881 Micrococcus flavus 98 Micrococcus sp. KBP.AS.404 50 100 Micrococcus sp. KBP.AS.252 NR 116578 Micrococcus yunnanensis 100 NR 044365 Micrococcus endophyticus
96 Micrococcus sp. KBP.AS.232 83 Micrococcus sp. KBP.AS.251 51 NR 024571 Brachybacterium muris
100 Brachybacterium sp. KBP.AS.478 50 NR 025503 Brachybacterium fresconis 54 AB184868 Streptomyces hydrogenans 97 NR 112382 Streptomyces odorifer 100 NR 043338 Streptomyces violascens Streptomyces sp. KBP.AS.261 NR 113310 Brevibacterium yomogidense 100 Brevibacterium luteolum KBP.AS.480 Actinobacteria 100 MK696423 Brevibacterium luteolum 100 KY928098 Brevibacterium epidermidis 100 81 83 NR 042458 Brevibacterium oceani NR 042586 Brevibacterium marinum 98 Brevibacterium sp. KBP.AS.514 Brevibacterium linens KBP.AS.238 94 Brevibacterium epidermidis KBP.AS.285 80 87 Brevibacterium sp. KBP.AS.240 94 KY307903 Brevibacterium linens 99 Leucobacter aridicollis KBP.AS.488 100 Leucobacter aridicollis KBP.AS.496 99 NR 042288 Leucobacter aridicollis Leucobacter sp. KBP.AS.513 100 Agrococcus jenensis KBP.AS.494 NR 026275 Agrococcus jenensis 100 96 Microbacterium pumilum KBP.AS.471 Microbacterium pumilum KBP.AS.472
98 Microbacterium sp. KBP.AS.392 100 JF778699 Microbacterium flavescens 56 Microbacterium flavescens KBP.AS.394 71 Microbacterium paraoxydans KBP.AS.275 100 NR 025548 Microbacterium paraoxydans Microbacterium paraoxydans KBP.AS.1275 MH321609 Microbacterium oxydans Microbacterium oxydans KBP.AS.256 Microbacterium sp. KBP.AS.242 65 Microbacterium oxydans KBP.AS.254 69 59 Microbacterium oxydans KBP.AS.397 58 Microbacterium oxydans KBP.AS.523 93 Corynebacterium mucifaciens KBP.AS.248 100 NR 026396 Corynebacterium mucifaciens Corynebacterium mucifaciens KBP.AS.246 98 NR 043009 Rhodococcus yunnanensis 75 LC130641 Rhodococcus cercidiphylli 100 99 Rhodococcus sp. KBP.AS.390 97 AB920569 Rhodococcus kyotonensis Rhodococcus sp. KBP.AS.269 100 NR 042801 Tsukamurella tyrosinosolvens 79 Tsukamurella tyrosinosolvens KBP.AS.723 73 Dietzia cinnamea KBP.AS.253 91 Dietzia cinnamea KBP.AS.495 100 NR 116462 Dietzia lutea NR 042390 Dietzia cinnamea
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NR 113801 Brevibacillus formosus 100 Brevibacillus brevis KBP.AS.483 NR 113767 Brevibacillus agri 100 Planomicrobium sp. KBP.AS.721 91 MK968152 Planomicrobium sp. 100 MN006530 Planomicrobium okeanokoites 100 Planomicrobium okeanokoites KBP.AS.722 Bacillus megaterium KBP.AS.272 63 Bacillus simplex KBP.AS.389 53 Bacillus simplex KBP.AS.475 98 Bacillus sp. KBP.AS.473 100 KF217249 Bacillus sp. Firmicutes 98 Bacillus sp. KBP.AS.481 55 Bacillus sp. KBP.AS.485 96 Bacillus sp. KBP.AS.112 82 Bacillus pumilus KBP.AS.231 62 Bacillus sp. KBP.AS.484 Bacillus sp. KBP.AS.271 60 Bacillus subtilis KBP.AS.247 KX785171 Bacillus licheniformis 57 NR 116187 Bacillus subtilis 82 NR 116185 Bacillus mojavensis 81 Bacillus sp. KBP.AS.110 72 NR 117611 Bacillus tequilensis 100 Chryseobacterium kwangjuense KBP.AS.519 100 NR 108175 Chryseobacterium kwangjuense Bacteroidetes LN681561 Chryseobacterium indologenes 75 Paracoccus marcusii KBP.AS.499 100 Paracoccus marcusii KBP.AS.237 93 NR 044922 Paracoccus marcusii 100 NR 113864 Paracoccus aminovorans 77 Paracoccus sp. KBP.AS.1266 Methylobacterium sp. KBP.AS.506 87 100 NR 074257 Methylorubrum populi
96 NR 042599 Ochrobactrum thiophenivorans Ochrobactrum thiophenivorans KBP.AS.1261 100 Ochrobactrum thiophenivorans KBP.AS.1264 100 Ochrobactrum sp. KBP.AS.113 69 60 Ochrobactrum thiophenivorans KBP.AS.1273 56 Ochrobactrum thiophenivorans KBP.AS.493 NR 042128 Sphingomonas aurantiaca
100 NR 146851 Sphingomonas panaciterrae 98 Sphingomonas sp. KBP.AS.249 93 100 NR 117177 Pseudomonas frederiksbergensis Proteobacteria 97 Pseudomonas frederiksbergensis KBP.AS.265 96 Pseudomonas sp. KBP.AS.282 Acinetobacter pittii KBP.AS.395 100 NR 117621 Acinetobacter pittii
100 Stenotrophomonas maltophilia KBP.AS.270 88 Stenotrophomonas sp. KBP.AS.220 50 Stenotrophomonas maltophilia KBP.AS.1265 100 Stenotrophomonas maltophilia KBP.AS.1268 Stenotrophomonas rhizophila KBP.AS.398 100 NR 028930 Stenotrophomonas rhizophila NR 121739 Stenotrophomonas rhizophila NR 112030 Stenotrophomonas maltophilia LT222224 Stenotrophomonas maltophilia 98 Stenotrophomonas maltophilia KBP.AS.1267 66 94 Stenotrophomonas maltophilia KBP.AS.401 54 Stenotrophomonas maltophilia KBP.AS.402
0.05 Figure S4. Phylogenetic analysis tree of 16S rRNA genes of the strains isolated from the Novaya Zemlya archipelago samples, which were sequenced using 1100r primer. The tree was constructed using the Neighbor‐Joining method with the use of the most closely related sequences from GenBank. The number of taxa—23. The number of significant characters—51. The number of bootstrap replicates—500. Confidence values of branching are given for cases of 50% and higher. Circles indicates the strains, isolated from the moraine sample [Nz 1], rhombus—strains isolated from the moraine and ice sample [Nz 2], triangles—isolated from the ice sample [Nz 3]. The scale under the tree indicates the number of phylogenetical significant changes of nucleotides.
Table S3. Physiological characteristics of isolated strains.
Temperature pH Max salt tolerance concentration Taxonomical Strain affiliation min max min max NaCl NaHCO3 MgSO4 KCl Mg(ClO4)2 KBP.AS.110 Bacillus sp. 25 25 7 8 5 <2 20 5 <0.5 KBP.AS.112 Bacillus sp. 25 25 6 8 10 <2 5 15 <0.5 KBP.AS.113 Ochrobactrum sp. 4 25 4 9 5 2 20 10 1
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Arthrobacter KBP.AS.122 ginsengisoli 10 25 6 8 10 <2 20 15 0.5 Ochrobactrum KBP.AS.1261 thiophenivorans 10 37 4 12 10 2 20 15 5 Arthrobacter KBP.AS.1262 ginsengisoli 2 37 4 12 10 2 20 15 5 KBP.AS.1263 Streptomyces sp. 2 37 6 12 10 2 20 15 5 Ochrobactrum KBP.AS.1264 thiophenivorans 2 37 6 12 15 2 20 15 5 Stenotrophomonas KBP.AS.1265 maltophilia 2 37 4 12 10 2 20 10 5 KBP.AS.1266 Paracoccus sp. 2 37 6 12 10 2 20 10 5 Stenotrophomonas KBP.AS.1267 maltophilia 10 25 6 8 2 <2 10 2 0.5 Stenotrophomonas KBP.AS.1268 maltophilia 4 25 5 9 2 <2 10 2 0.5 Stenotrophomonas KBP.AS.1269 sp. 10 37 6 8 2 <2 20 2 <0.5 Stenotrophomonas KBP.AS.1270 maltophilia 4 25 5 9 2 <2 10 <2 0.5 KBP.AS.1271 Enterobacter sp. 10 25 4 8 5 <2 20 2 <0.5 Stenotrophomonas KBP.AS.1272 maltophilia 4 37 5 9 2 <2 10 2 1 Ochrobactrum KBP.AS.1273 thiophenivorans 4 25 6 9 10 <2 15 <2 0.5 Microbacterium KBP.AS.1275 paraoxydans 10 25 6 8 2 <2 10 2 0.5 Stenotrophomonas KBP.AS.220 sp. 25 37 6 12 10 20 20 10 10 KBP.AS.230 Micrococcus sp. 10 25 7 8 10 <2 5 5 1 KBP.AS.231 Bacillus pumilus 25 25 6 8 5 <2 5 10 <0.5 KBP.AS.232 Micrococcus sp. 25 25 7 9 10 <2 5 10 0.5 KBP.AS.233 Rhodococcus sp. 25 25 6 8 2 <2 20 5 1 KBP.AS.234 Bacillus infantis 25 25 6 8 5 <2 5 5 <0.5 KBP.AS.235 Streptomyces sp. 25 25 5 8 10 <2 5 15 2 KBP.AS.236 Arthrobacter agilis 2 25 5 11 5 <2 5 10 2 KBP.AS.237 Paracoccus marcusii 25 25 6 8 2 <2 5 5 0.5 KBP.AS.238 Brevibacterium linens 10 25 7 10 5 <2 5 5 1 KBP.AS.239 Micrococcus terreus 10 25 6 11 10 <2 5 10 <0.5 KBP.AS.240 Brevibacterium sp. 25 25 5 8 15 <2 20 20 <0.5 KBP.AS.241 Streptomyces sp. 25 25 6 8 10 <2 5 10 1 KBP.AS.242 Microbacterium sp. 10 25 6 8 5 <2 20 15 1 KBP.AS.244 Pseudomonas sp. 25 25 6 8 <2 <2 <2 <2 <0.5 Stenotrophomonas KBP.AS.245 maltophilia 10 25 6 12 10 <2 5 10 <0.5
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Corynebacterium KBP.AS.246 mucifaciens 25 25 6 8 10 <2 20 15 1 KBP.AS.247 Bacillus subtilis 25 50 5 8 10 <2 20 15 1 Corynebacterium KBP.AS.248 mucifaciens 25 25 6 8 10 <2 10 20 1 KBP.AS.249 Sphingomonas sp. 25 25 7 8 <2 <2 <2 2 1 KBP.AS.250 Bacillus sp. 10 37 3 8 15 <2 20 20 <0.5 KBP.AS.251 Micrococcus sp. 25 37 3 8 15 <2 15 15 2 KBP.AS.252 Micrococcus sp. 10 37 3 9 2 <2 15 15 2 KBP.AS.253 Dietzia cinnamea 25 25 6 8 2 2 <2 <2 <0.5 Microbacterium KBP.AS.254 oxydans 2 37 7 8 10 <2 20 15 10 KBP.AS.255 Micrococcus luteus 25 45 3 8 15 <2 20 20 0.5 Microbacterium KBP.AS.256 oxydans 10 37 6 9 5 2 20 10 5 KBP.AS.257 Streptomyces sp. 10 37 4 8 5 <2 20 15 2 Arthrobacter KBP.AS.258 crystallopoietes 10 45 3 8 15 <2 20 15 1 KBP.AS.259 Streptomyces sp. 10 37 4 8 10 <2 20 15 2 KBP.AS.261 Streptomyces sp. 10 37 3 8 15 <2 20 15 2 KBP.AS.262 Bacillus sp. 10 37 4 10 15 2 20 15 5 Pseudomonas KBP.AS.265 frederiksbergensis 10 45 4 8 5 <2 20 10 2 KBP.AS.267 Glutamicibacter sp. 10 37 4 8 5 <2 20 10 2 KBP.AS.269 Rhodococcus sp. 10 37 4 8 5 <2 20 10 5 Stenotrophomonas KBP.AS.270 maltophilia 2 37 4 12 10 2 20 10 5 KBP.AS.271 Bacillus sp. 25 37 4 10 15 2 15 20 5 KBP.AS.272 Bacillus megaterium 10 45 4 8 5 <2 20 10 1 Microbacterium KBP.AS.275 paraoxydans 2 25 5 11 2 <2 10 2 1 KBP.AS.282 Pseudomonas sp. 10 45 3 12 10 2 20 10 2 Arthrobacter KBP.AS.283 ginsengisoli 4 37 6 9 <2 <2 2 5 <0.5 Pseudarthrobacter KBP.AS.284 scleromae 25 37 4 10 10 2 15 5 2 Brevibacterium KBP.AS.285 epidermidis 25 45 4 10 10 2 10 5 2 KBP.AS.389 Bacillus simplex 2 37 6 12 5 2 20 10 5 KBP.AS.390 Rhodococcus sp. 2 37 6 9 10 5 20 15 5 KBP.AS.391 Klebsiella sp. 4 25 6 12 2 <2 15 5 <0.5 KBP.AS.392 Microbacterium sp. 2 37 4 8 5 5 15 5 2 Arthrobacter KBP.AS.393 sulfonivorans 2 25 4 8 5 <2 20 10 2
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Microbacterium KBP.AS.394 flavescens 2 37 6 12 5 5 20 10 5 KBP.AS.395 Acinetobacter pittii 2 25 3 12 5 2 15 10 5 KBP.AS.396 Bacillus sp. 10 37 3 12 10 2 20 15 5 Microbacterium KBP.AS.397 oxydans 4 37 6 12 10 2 20 10 5 Stenotrophomonas KBP.AS.398 rhizophila 4 37 6 12 15 2 20 15 5 KBP.AS.399 Microbacterium sp. 10 37 5 12 5 <2 20 5 <0.5 KBP.AS.400 Pseudomonas putida 2 37 4 11 10 <2 20 10 1 Stenotrophomonas KBP.AS.401 maltophilia 10 37 5 12 5 <2 20 5 <0.5 Stenotrophomonas KBP.AS.402 maltophilia 10 37 4 12 5 <2 20 5 1 KBP.AS.403 Rhodococcus sp. 2 37 6 12 5 5 20 10 2 KBP.AS.404 Micrococcus sp. 4 37 6 12 10 2 20 10 5 KBP.AS.413 Kocuria rosea 10 25 7 8 2 <2 <2 <2 <0.5 KBP.AS.414 Bacillus sp. 4 37 4 12 15 2 20 20 5 Pseudomonas KBP.AS.417 frederiksbergensis 25 25 5 8 5 <2 5 5 0.5 Microbacterium KBP.AS.471 pumilum 4 37 6 12 10 2 20 10 5 Microbacterium KBP.AS.472 pumilum 25 50 6 8 2 <2 5 10 <0.5 KBP.AS.473 Bacillus sp. 25 50 6 8 <2 <2 <2 5 <0.5 Pseudomonas KBP.AS.474 frederiksbergensis 25 25 6 8 <2 <2 <2 <2 <0.5 KBP.AS.475 Bacillus simplex 2 37 6 8 5 5 10 10 10 KBP.AS.476 Pseudomonas sp. 2 37 5 12 10 <2 20 10 2 KBP.AS.477 Micrococcus sp. 10 50 6 8 2 5 5 10 5 KBP.AS.478 Brachybacterium sp. 4 37 4 12 5 2 20 10 5 Brevibacterium KBP.AS.479 aureum 10 25 5 10 10 <2 5 15 0.5 Brevibacterium KBP.AS.480 luteolum 25 45 3 8 20 <2 20 20 2 KBP.AS.481 Bacillus sp. 10 45 3 8 15 <2 20 20 2 KBP.AS.482 Bacillus sp. 10 45 3 8 15 <2 20 20 2 KBP.AS.483 Brevibacillus brevis 10 37 6 12 10 2 20 10 5 KBP.AS.484 Bacillus sp. 10 37 5 12 5 2 20 5 0.5 KBP.AS.485 Bacillus sp. 25 37 6 8 <2 <2 2 10 <0.5 KBP.AS.486 Pseudarthrobacter sp. 25 25 6 8 <2 <2 <2 <2 <0.5 KBP.AS.487 Pseudarthrobacter sp. 2 50 6 8 2 2 15 10 5 Leucobacter KBP.AS.488 aridicollis 25 37 4 12 2 <2 20 5 1
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KBP.AS.489 Pseudochrobactrum sp. 10 50 6 8 <2 2 5 10 5 KBP.AS.490 Pseudomonas sp. 2 25 5 8 2 <2 15 2 0.5 KBP.AS.491 Kocuria rosea 2 25 5 8 5 <2 20 2 0.5 Arthrobacter KBP.AS.492 ginsengisoli 25 25 3 8 15 <2 20 15 0.5 Ochrobactrum KBP.AS.493 thiophenivorans 25 50 5 10 5 <2 20 <2 <0.5 KBP.AS.494 Agrococcus jenensis 10 45 3 8 15 <2 20 20 1 KBP.AS.495 Dietzia cinnamea 25 45 3 8 15 <2 20 20 1 Leucobacter KBP.AS.496 aridicollis 25 25 3 8 15 <2 20 20 2 KBP.AS.497 Micrococcus sp. 10 45 3 8 15 <2 20 20 <0.5 KBP.AS.498 Bacillus sp. 10 45 3 8 15 <2 20 20 1 KBP.AS.499 Paracoccus marcusii 25 25 6 8 <2 2 <2 <2 <0.5 Staphylococcus KBP.AS.500 epidermidis 2 45 6 8 <2 2 10 20 5 KBP.AS.501 Bacillus sp. 25 45 4 12 10 <2 2 <2 <0.5 Staphylococcus KBP.AS.502 epidermidis 10 45 3 8 15 <2 20 20 1 KBP.AS.503 Micrococcus terreus 10 45 3 8 15 <2 20 20 1 KBP.AS.505 Dietzia cinnamea 10 50 6 8 <2 <2 5 15 <0.5
KBP.AS.506 Methylobacterium sp. 10 25 5 8 2 <2 5 2 <0.5 KBP.AS.511 Arthrobacter sp. 2 45 4 12 10 2 20 10 2 KBP.AS.513 Leucobacter sp. 10 25 6 12 2 <2 2 2 <0.5 KBP.AS.514 Brevibacterium sp. 10 37 5 11 5 <2 20 5 5 KBP.AS.516 Pseudomonas sp. 4 37 6 12 20 2 20 20 5 Chryseobacterium KBP.AS.519 kwangjuense 10 25 5 8 <2 2 2 5 <0.5 KBP.AS.521 Streptomyces sp. 10 25 6 8 2 2 <2 2 <0.5 KBP.AS.522 Streptomyces sp. 10 25 4 8 5 2 20 5 <0.5 Microbacterium KBP.AS.523 oxydans 4 25 6 9 2 2 10 <2 0.5 Pseudarthrobacter KBP.AS.525 siccitolerans 4 37 6 12 10 2 20 10 5 Pseudomonas KBP.AS.526 oryzihabitans 10 37 5 12 5 <2 20 5 1 KBP.AS.721 Planomicrobium sp. 25 37 5 8 10 <2 20 15 1 Planomicrobium KBP.AS.722 okeanokoites 25 25 5 8 10 <2 5 15 1 Tsukamurella KBP.AS.723 tyrosinosolvens 25 37 5 8 10 <2 20 15 1 KBP.AS.881 Arthrobacter sp. 2 45 4 10 10 2 20 10 5 Arthrobacter KBP.AS.882 ginsengisoli 25 37 6 12 2 <2 <2 <2 <0.5
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KBP.AS.885 Pseudomonas sp. 10 25 5 11 2 <2 20 2 0.5 KBP.AS.886 Pseudomonas sp. 4 37 6 10 10 5 5 5 0.5 Arthrobacter KBP.AS.887 ginsengisoli 4 37 6 12 10 2 20 15 5 Arthrobacter KBP.AS.894 ginsengisoli 4 37 4 12 10 2 20 10 5
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Table S4. Antibiotics resistance spectra of isolated strains.
Strain Taxonomical affiliation Amp Chl Rif Tet Kan Dox Ceph KBP.AS.110 Bacillus sp. S S S S S S S KBP.AS.112 Bacillus sp. S S S S S S S KBP.AS.113 Ochrobactrum sp. R R R S R S R KBP.AS.122 Arthrobacter ginsengisoli S S R S S S S KBP.AS.1261 Ochrobactrum thiophenivorans R R S R S S R KBP.AS.1262 Arthrobacter ginsengisoli R R S R S S R KBP.AS.1263 Streptomyces sp. R R S R S S R KBP.AS.1264 Ochrobactrum thiophenivorans R S S R S S R KBP.AS.1265 Stenotrophomonas maltophilia R R S R S S R KBP.AS.1266 Paracoccus sp. R R S R S S R KBP.AS.1267 Stenotrophomonas maltophilia S S S S S R S KBP.AS.1268 Stenotrophomonas maltophilia S S S S S S S KBP.AS.1269 Stenotrophomonas sp. S S S R S S S KBP.AS.1270 Stenotrophomonas maltophilia S S S S S S S KBP.AS.1271 Enterobacter sp. S S S S S S S KBP.AS.1272 Stenotrophomonas maltophilia S R S S S S S KBP.AS.1273 Ochrobactrum thiophenivorans S S S S S R S KBP.AS.1275 Microbacterium paraoxydans S S S S S S S KBP.AS.220 Stenotrophomonas sp. S R S S S S R KBP.AS.230 Micrococcus sp. S S S S S S S KBP.AS.231 Bacillus pumilus S S S S S S S KBP.AS.232 Micrococcus sp. S S S S S S S KBP.AS.233 Rhodococcus sp. S S S S S S S KBP.AS.234 Bacillus infantis S S S S S S S KBP.AS.235 Streptomyces sp. R S S S S S S KBP.AS.236 Arthrobacter agilis S S R S S S S KBP.AS.237 Paracoccus marcusii S S S S S S S KBP.AS.238 Brevibacterium linens S R S S S S R KBP.AS.239 Micrococcus terreus S R S S S S R KBP.AS.240 Brevibacterium sp. S R R S S S S KBP.AS.241 Streptomyces sp. S S S S S S S KBP.AS.242 Microbacterium sp. R R S S S R R KBP.AS.244 Pseudomonas sp. S S S S S S S KBP.AS.245 Stenotrophomonas maltophilia S R S R S S R KBP.AS.246 Corynebacterium mucifaciens S S S S S S S
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KBP.AS.247 Bacillus subtilis S S S S S S S KBP.AS.248 Corynebacterium mucifaciens S S S S S S S KBP.AS.249 Sphingomonas sp. S S S S S S S KBP.AS.250 Bacillus sp. R S S S S S R KBP.AS.251 Micrococcus sp. R S S R S R R KBP.AS.252 Micrococcus sp. R S S S S S R KBP.AS.253 Dietzia cinnamea S S S S S S S KBP.AS.254 Microbacterium oxydans S R S R S S S KBP.AS.255 Micrococcus luteus S S S S S S R KBP.AS.256 Microbacterium oxydans S R S R S S S KBP.AS.257 Streptomyces sp. R S S S S S R KBP.AS.258 Arthrobacter crystallopoietes R S S S S S R KBP.AS.259 Streptomyces sp. S S S S S S R KBP.AS.261 Streptomyces sp. R S S S S R R KBP.AS.262 Bacillus sp. R R S R R R S KBP.AS.265 Pseudomonas frederiksbergensis S S S S S S R KBP.AS.267 Glutamicibacter sp. R S S S S S S KBP.AS.269 Rhodococcus sp. S S S S S S S KBP.AS.270 Stenotrophomonas maltophilia R R S R S S R KBP.AS.271 Bacillus sp. S S S S S S S KBP.AS.272 Bacillus megaterium R R S S S S R KBP.AS.275 Microbacterium paraoxydans R R R S R S R KBP.AS.282 Pseudomonas sp. R R S S R R S KBP.AS.283 Arthrobacter ginsengisoli S S S S S S S KBP.AS.284 Pseudarthrobacter scleromae R S S S S S S KBP.AS.285 Brevibacterium epidermidis R S S S S S S KBP.AS.389 Bacillus simplex S R S R S R S KBP.AS.390 Rhodococcus sp. S R S R S R S KBP.AS.391 Klebsiella sp. S S S S S S S KBP.AS.392 Microbacterium sp. S R S S R S R KBP.AS.393 Arthrobacter sulfonivorans S R S S R S R KBP.AS.394 Microbacterium flavescens S R S S S S S KBP.AS.395 Acinetobacter pittii R R S S R S R KBP.AS.396 Bacillus sp. S R S S S S S KBP.AS.397 Microbacterium oxydans R R S R S S R KBP.AS.398 Stenotrophomonas rhizophila R R S R S S R KBP.AS.399 Microbacterium sp. S S S S S S S
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KBP.AS.400 Pseudomonas putida R R S S S R R KBP.AS.401 Stenotrophomonas maltophilia S S S S S S S KBP.AS.402 Stenotrophomonas maltophilia R R S R R S R KBP.AS.403 Rhodococcus sp. S R S R S R S KBP.AS.404 Micrococcus sp. R R S R S S R KBP.AS.413 Kocuria rosea S S S S S S S KBP.AS.414 Bacillus sp. R R S R S S R KBP.AS.417 Pseudomonas frederiksbergensis R R R R S S S KBP.AS.471 Microbacterium pumilum R R S R S S R KBP.AS.472 Microbacterium pumilum S S S S S S R KBP.AS.473 Bacillus sp. S S S S S S S KBP.AS.474 Pseudomonas frederiksbergensis S S S S S S S KBP.AS.475 Bacillus simplex S S S S S S S KBP.AS.476 Pseudomonas sp. R R S S R S R KBP.AS.477 Micrococcus sp. S S R S S S S KBP.AS.478 Brachybacterium sp. R R S R R R S KBP.AS.479 Brevibacterium aureum S R S S S S S KBP.AS.480 Brevibacterium luteolum S S S S S S S KBP.AS.481 Bacillus sp. S S S S S R R KBP.AS.482 Bacillus sp. S S S S S R S KBP.AS.483 Brevibacillus brevis R R S R S R R KBP.AS.484 Bacillus sp. S S S R S R S KBP.AS.485 Bacillus sp. S S S S S S S KBP.AS.486 Pseudarthrobacter sp. S S S S S S S KBP.AS.487 Pseudarthrobacter sp. S S R S S S S KBP.AS.488 Leucobacter aridicollis R R S S R S R KBP.AS.489 Pseudochrobactrum sp. R S S S S S S KBP.AS.490 Pseudomonas sp. R R S S S S R KBP.AS.491 Kocuria rosea R R S S S S R KBP.AS.492 Arthrobacter ginsengisoli R S S R S R R KBP.AS.493 Ochrobactrum thiophenivorans R R S R S S S KBP.AS.494 Agrococcus jenensis R S S S S S R KBP.AS.495 Dietzia cinnamea S S S R S R R KBP.AS.496 Leucobacter aridicollis R S S R S R R KBP.AS.497 Micrococcus sp. R S S R S R R KBP.AS.498 Bacillus sp. S S S S S S R KBP.AS.499 Paracoccus marcusii S S S S S S S
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KBP.AS.500 Staphylococcus epidermidis S S S S S S R KBP.AS.501 Bacillus sp. S S S S S S S KBP.AS.502 Staphylococcus epidermidis S S S S S S R KBP.AS.503 Micrococcus terreus R S S S S S R KBP.AS.505 Dietzia cinnamea S S S S S S R KBP.AS.506 Methylobacterium sp. S R S S S S S KBP.AS.511 Arthrobacter sp. R R S S R S S KBP.AS.513 Leucobacter sp. S S S S S S S KBP.AS.514 Brevibacterium sp. S S S S R S R KBP.AS.516 Pseudomonas sp. R S S R S S R KBP.AS.519 Chryseobacterium kwangjuense S S S S S S R KBP.AS.521 Streptomyces sp. S S S S S S S KBP.AS.522 Streptomyces sp. S S S S S S S KBP.AS.523 Microbacterium oxydans S S S S S S S KBP.AS.525 Pseudarthrobacter siccitolerans R R S R S S R KBP.AS.526 Pseudomonas oryzihabitans S S S S S S S KBP.AS.721 Planomicrobium sp. S S S S S S S KBP.AS.722 Planomicrobium okeanokoites S S S S S S S KBP.AS.723 Tsukamurella tyrosinosolvens S S S S S S S KBP.AS.881 Arthrobacter sp. R R S R S S S KBP.AS.882 Arthrobacter ginsengisoli S S S S S S S KBP.AS.885 Pseudomonas sp. S S S S S S S KBP.AS.886 Pseudomonas sp. S S S S S S S KBP.AS.887 Arthrobacter ginsengisoli R R S S S S R KBP.AS.894 Arthrobacter ginsengisoli R R S R S S R
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