ECBI0060.Pdf

Total Page:16

File Type:pdf, Size:1020Kb

ECBI0060.Pdf UNIVERSIDAD DE INVESTIGACIÓN DE TECNOLOGÍA EXPERIMENTAL YACHAY Escuela de Ciencias Biológicas e Ingeniería TÍTULO: Sugarcane byproducts potential uses and vinasse as feedstock to generate bioenergy Trabajo de integración curricular presentado como requisito para la obtención del título de Ingeniero Biomédico Autor: Aldaz Luna Bryan Patricio Tutor: Ph.D. Santiago Vispo Nelson Urcuquí, agosto 2020 Urcuquí, 2 de diciembre de 2020 SECRETARÍA GENERAL ( Vicerrectorado Académico/Cancillería ) ESCUELA DE CIENCIAS BIOLÓGICAS E INGENIERÍA CARRERA DE BIOMEDICINA ACTA DE DEFENSA No. UITEY-BIO-2020-00043-AD A los 2 días del mes de diciembre de 2020, a las 16:30 horas, de manera virtual mediante videoconferencia, y ante el Tribunal Calificador, integrado por los docentes: Presidente Tribunal de Defensa Dra. SPENCER VALERO, LILIAN MARITZA , Ph.D. Miembro No Tutor Dra. RODRIGUEZ CABRERA, HORTENSIA MARIA , Ph.D. Tutor Dr. SANTIAGO VISPO, NELSON FRANCISCO , Ph.D. El(la) señor(ita) estudiante ALDAZ LUNA, BRYAN PATRICIO, con cédula de identidad No. 0704398759, de la ESCUELA DE CIENCIAS BIOLÓGICAS E INGENIERÍA, de la Carrera de BIOMEDICINA, aprobada por el Consejo de Educación Superior (CES), mediante Resolución RPC-SO-43-No.496-2014, realiza a través de videoconferencia, la sustentación de su trabajo de titulación denominado: SUGARCANE BYPRODUCTS POTENTIAL USES AND VINASSE AS FEEDSTOCK TO GENERATE BIOENERGY, previa a la obtención del título de INGENIERO/A BIOMÉDICO/A. El citado trabajo de titulación, fue debidamente aprobado por el(los) docente(s): Tutor Dr. SANTIAGO VISPO, NELSON FRANCISCO , Ph.D. Y recibió las observaciones de los otros miembros del Tribunal Calificador, las mismas que han sido incorporadas por el(la) estudiante. Previamente cumplidos los requisitos legales y reglamentarios, el trabajo de titulación fue sustentado por el(la) estudiante y examinado por los miembros del Tribunal Calificador. Escuchada la sustentación del trabajo de titulación a través de videoconferencia, que integró la exposición de el(la) estudiante sobre el contenido de la misma y las preguntas formuladas por los miembros del Tribunal, se califica la sustentación del trabajo de titulación con las siguientes calificaciones: Tipo Docente Calificación Presidente Tribunal De Defensa Dra. SPENCER VALERO, LILIAN MARITZA , Ph.D. 10 , 0 Miembro Tribunal De Defensa Dra. RODRIGUEZ CABRERA, HORTENSIA MARIA , Ph.D. 10 , 0 Tutor Dr. SANTIAGO VISPO, NELSON FRANCISCO , Ph.D. 10 , 0 Lo que da un promedio de: 10 (Diez punto Cero), sobre 10 (diez), equivalente a: APROBADO II Para constancia de lo actuado, firman los miembros del Tribunal Calificador, el/la estudiante y el/la secretario ad-hoc. Certifico que en cumplimiento del Decreto Ejecutivo 1017 de 16 de marzo de 2020, la defensa de trabajo de titulación (o examen de grado modalidad teórico práctica) se realizó vía virtual, por lo que las firmas de los miembros del Tribunal de Defensa de Grado, constan en forma digital. ALDAZ LUNA, BRYAN PATRICIO Estudiante Dra. SPENCER VALERO, LILIAN MARITZA , Ph.D. Presidente Tribunal de Defensa Hacienda San José s/n y Proyecto Yachay, Urcuquí | Tlf: +593 6 2 999 500 | [email protected] www.yachaytech.edu.ec Dr. SANTIAGO VISPO, NELSON FRANCISCO , Ph.D. Tutor Dra. RODRIGUEZ CABRERA, HORTENSIA MARIA , Ph.D. Miembro No Tutor ALARCON FELIX, KARLA ESTEFANIA Secretario Ad-hoc III IV V DEDICATORIA Quisiera dedicar este trabajo final a mis padres, por todo el apoyo brindado a lo largo de estos cinco años en Yachay Tech. A todos mis amigos que hicieron de la universidad mi segundo hogar y un lugar mejor, Cristhian, Demetrio, Freddy, Hector, Isaac, Luis, Sebastian, David, Milena, Maria Fernanda, Maria Daniela, y Fernanda. A mis amigos con lo que no perdi contacto y estuvieron apoyándome a la distancia, Axel, Debbie, Victor, Michael, Andres y Paul. Un pedazo de este trabajo es de todos ustedes. VI AGRADECIMIENTOS Quisiera agradecer a todos mis profesores en Yachay Tech. Desde que inicié mi etapa universitaria ustedes aportaron de la mejor manera a mi formación profesional y personal. Gracias por todos los consejos, enseñanzas, lecciones y regaños que me dieron en estos últimos años. Especialmente, muchas gracias a mi tutor Nelson Santiago Vispo y a mi profesor Carlos Esteban Pazmiño por su ayuda y mentoría a lo largo de este trabajo. VII Resumen Hoy en día, los ingenios azucareros usan la caña de azúcar y su jugo para obtener productos como la azúcar de mesa, miel y etanol. A lo largo de los diferentes procesos de obtención, muchos desperdicios se producen impactando negativamente a suelos y ríos. Los principales desechos de la industria azucarera son el bagazo, la paja, la cachaza y la vinaza. Gracias a los avances en procesos biotecnológicos, de la mano de la biorefinería, estos desechos pueden ser vistos y tratados como subproductos dándoles un valor agregado y produciendo energía, por ejemplo biogás, la cual puede ser aprovechada económicamente. Al mismo tiempo, el aprovechamiento de estos subproductos ayuda a mitigar el impacto negativo al medioambiente. En específico, la vinaza es el principal subproducto líquido de la fermentación del jugo de la caña de azúcar para producir etanol. En Ecuador, aproximadamente 1.000.000 m3 de vinaza son producidos anualmente generando una gran cantidad de desperdicios. Sin embargo, esta enorme cantidad de vinaza puede ser vista como materia prima para obtener biogás. En esta revisión bibliográfica, se reportan los resultados obtenidos de la simulación de un modelo simplificado de digestión anaerobia, basado en BNRM No. 2 por sus siglas en inglés para Biological Nutrient Removal Model, de la vinaza para producir metano y posteriormente biogás en un reactor tipo Batch. La concentración de metano fue de 22,9931.72 mg/L y la remoción de la carga orgánica fue del 99.96 % en 0.66 días de proceso. Con estos resultados, se pueden obtener aproximadamente 10,416.65 kg de metano lo cual se traduce en 103,056.75 kW.h y a la vez como $10,233.54. Palabras clave: Caña de azúcar, subproductos, vinaza, bioenergía, biogás. VIII Abstract Around the world, the sugarcane industries are obtaining products such as table sugar, honey, and ethanol. At the same time, these industries are generating many wastes that impact negatively on soils and rivers. Bagasse, straw, cachaça, and vinasse are the primary wastes of the sugar industry. With the proper biotechnological processes, hand in hand with the biorefinery, these wastes can be seen and treated as byproducts giving them an added value producing bioenergy that can be economically exploited. Furthermore, the use of these byproducts helps to mitigate the negative impact on the environment. Specifically, vinasse is the main liquid subproduct of the fermentation of sugarcane juice to produce ethanol. In Ecuador, approximately 1,000,000 m3 of vinasse are produced per year, generating a large amount of waste. Vinasse can be seen as a raw material for producing biogas. In this thesis, the results obtained from the simulation of a simplified model, based on Biological Nutrient Removal Model 2 (BNRM No.2), for anaerobic digestion of vinasse to produce methane and later biogas in a batch reactor are reported. Methane concentration was 22,931.72 mg/L, with 99.96% of organic matter degradation in 0.66 days of the process. With these results, 10,416.65 kg of methane can be attained, which translates into 103,056.75 kWh, which means a profit of $ 10,233.54. Keywords: Sugarcane, byproducts, vinasse, bioenergy, biogas. IX Content Resumen................................................................................................................................... VIII Abstract .......................................................................................................................................IX List of Figures ............................................................................................................................XII List of Tables ............................................................................................................................XIII List of Annexes .........................................................................................................................XIII 1. Introduction ..........................................................................................................................1 Chapter 2 ......................................................................................................................................4 2.1. Hypothesis .............................................................................................................................4 2.2. General Objective .............................................................................................................4 2.3. Specific Objectives ...........................................................................................................4 Chapter 3 ......................................................................................................................................6 3. Sugar Industry Feedstock .....................................................................................................6 3.1. Sugarcane, (Saccharum officinarum) ...............................................................................6 3.2. Sugarcane Bagasse and Straw ..........................................................................................8 3.3. Filter Cake (Cachaça) and Honey .....................................................................................9
Recommended publications
  • Significance of Vinasses Waste Management in Agriculture and Environmental Quality- Review
    Vol. 9(38), pp. 2862-2872, 18 September, 2014 DOI: 10.5897/AJAR2014.8819 Article Number: 94224DC47396 African Journal of Agricultural ISSN 1991-637X Copyright © 2014 Research Author(s) retain the copyright of this article http://www.academicjournals.org/AJAR Full Length Research Paper Significance of vinasses waste management in agriculture and environmental quality- Review Rajagopal Vadivel*, Paramjit Singh Minhas, Suresh Kumar P., Yogeswar Singh, Nageshwar Rao D.V.K. and Avinash Nirmale National Institute of Abiotic Stress Management, Malegaon Khurd, Baramati taluk, Pune- 413115 Maharashtra, India. Received 4 May, 2014; Accepted 16 June, 2014 Vinasse is a waste material from distillery industries which has lot of organic and inorganic loads. It is utilized in agriculture for cheap nutrients source, ameliorating agents and animal feed beyond the limitation of high biochemical oxygen demand (BOD; 46100 to 96000 mgL-1), chemical oxygen demand (COD; 104000 to 134400 mgL-1) and total dissolved salts (30.5 to 45.2 dSm-1) content even after the pollutant removal treatments. Vinasse treatments with combined approach of aerobic and anaerobic methods are more effective by both cost and pollutant on removal efficiency. Optimized dose of vinasse application has significance over soil properties, crop qualities and yield improvement. Globally, it has high potential to substitute potassium and nitrogen nutrients to the present level of annual consumption. It also contributes a substantial amount of phosphorous, calcium, sulphur and micronutrients to crops. In developed countries, starchy vinasse used as animal feed on a lean season to animals that improved the feed digestibility and animal quality where feed shortages are experienced.
    [Show full text]
  • Better Sugar; Better Business Mill Issues and Co-Products
    BETTER SUGAR; BETTER BUSINESS MILL ISSUES AND CO-PRODUCTS M. Regis Lima Verde Leal WWF WORKSHOP - LONDON, JUNE 23-24, 2005 WORLD CROP LAND CROP AREA (MMHA) Sugar cane 20.1 Wheat 207.5 Rice 153.0 Maize 144.8 Soybeans 91.6 Notes: Rice - 42,5MMha in India and 29,4mmha in China Wheat - 27,3mmha in India and 21,7mmha in China Corn - 29,7MMha in USA and 25,6mmha in China Source: FAO, 2004 MAIN SUGAR CANE PRODUCERS COUNTRY AREA SUGAR CANE (1000HA) (1000 TONNES) Brazil 5,455 411,009 India 4,100 244,800 China 1,316 93,200 Thailand 1,050 63,707 Pakistan 1,049 52,040 Cuba 700 24,000 Mexico 639 45,126 Australia 415 36,892 Other 5,377 347,097 TOTAL 20,100 1,317,871 Source: FAO, 2004 BRAZILIAN CROP LAND AND PRODUCTION CROP AREA PRODUCTION (MMHA) (MM TONNES) Soy Beans 21.5 49.5 Corn 12.3 41.8 Sugar Cane 5.6 416.3 Beans 4.0 3.0 Rice 3.7 13.3 Wheat 2.8 5.7 Coffee 2.4 2.5 Other 5.7 - TOTAL 58.0 - Source: IBGE, 2004 BRAZIL LAND USE TYPE AREA (MMHA) Total Country 851 Brazilian Native Amazon 370 Secondary Amazon and other Native Forests 180 Planted Forests (cellulose and energy) 6 Pasture 197 Arable Land 59 Permanent Crop 7.6 Agricultural Area 263 Land for Crops/Low Impacts (*) 90 Source: FAO, 2002 and EMBRAPA (*) BRAZILIAN MODEL FOR SUGAR CANE INDUSTRY •JOINT PRODUCTION OF SUGAR AND ETHANOL 1990 2002 Sugar only mills 27 15 Ethanol distilleries 180 104 Sugar/Ethanol mills 168 199 Total 375 318 •LAND OWNERSHIP Mills = 65% Independent Cane Growers (70,000) = 35% •HARVESTING SEASON Center-South : May - November North-Northeast: September - March •MILL SIZE:
    [Show full text]
  • Tropical Maize and Lipid Cane As Sustainable Bioenergy Crops
    Tropical Maize and Lipid Cane As Sustainable Bioenergy Crops Vijay Singh University of Illinois RCN Conference on Pan American Biofuels & Bioenergy Sustainability July 22-25, 2014 Golden Tulip Recife Palace Recife, Brazil What is Tropical Maize? Tropical Maize is Corn Reproductive Asynchrony Tropical Maize = Tropical x Temperate Maize Hybrid High Biomass High Stalk Sugars Less Nitrogen requirement History of tropical maize From Stoskopf, 1981 Nutrient Use Efficiency TM produces more biomass and more sugar than commercial corn hybrids with < 50% N fertilizer requirement US Sweetener Industry Tropical Maize Preparing the syrup by using a press HPLC profile of the tropical maize syrup. Three main sugars content—Sucrose, Glucose and Fructose Chen, M., Kaur, P., Dien, B.S., Below, F.E., Vincent, M.L. and Singh, V. 2013. Accumulation of fermentation sugars during tropical maize development. World Journal of Microbiology and Biotechnology 29:1509-1515. Tropical Maize 10 8 6 4 YEPS Ethanol concentration (% v/v) 2 corn syrup (autoclaved) corn syrup (autoclaved; pH4.5) corn syrup (initial) 0 0 20406080 Fermentation time (hour) Chen, M., Kaur, P., Dien, B.S., Below, F.E., Vincent, M.L. and Singh, V. 2013. 7 Accumulation of fermentation sugars during tropical maize development. World Journal of Fig.1. The ethanol production from maize syrup and YEPS medium Microbiology and Biotechnology 29:1509-1515. Tropical Maize 18 15.6 16 14.5 14 12.7 12 10.0 10 8.1 8 6 1.00x corn syrup 4 1.25x corn syrup Ethanol concentration (% v/v) (% concentration Ethanol 1.50x corn syrup 2 1.75x corn syrup 2.25x corn syrup 0 0 20406080100 Fermentation time (hour) Fig.
    [Show full text]
  • Maximizing Fuel Ethanol Opportunities Through Advanced Technologies PETROFED-May 2016 Mahesh Kulkarni, Sr
    Maximizing Fuel Ethanol Opportunities through Advanced Technologies PETROFED-May 2016 Mahesh Kulkarni, Sr. GM-Technology & Engineering Praj Industries Ltd., Pune © Praj Industries Ltd www.praj.net Who We Are Technology Backed References Across Solutions Five Continents A Knowledge Based A Global Indian Company Company End-to-end A Customer Solution Provider Centric Company Expertise and Experience in Process and Integration Customized Solutions Engineering A Socially Responsible Corporate Citizen Strong Focus on Sustainable Development © Praj Industries Ltd www.praj.net 2 Why PRAJ 1 Praj Matrix Engineering 2 Over 1000 employees of which 80,000 sq ft of Labs, Pilot Plants, and Offices 85% are engineers. Capability to design to 115 technologists - 30 PhDs, 80 Masters. international standards Follow global engineering 9 Technology Centers of Excellence - Biology, standards Chemistry, Engineering Skid engineering 1 TPD Cellulosic Ethanol Adequate knowledge of latest pilot plant design & simulation software such as P4D, PDMS, Chemcad ,Aspen & HTRI Technical Audit 3 manufacturing units Trouble shooting connected highways & ports having total manufacturing Spares Supply capacity of 13500 T/yr Supply of Equipment ASME “U” & “H” certified Plant/ Equipment Life Can supply equipment with Extension Services “CE” certification Preventive Maintenance ISO 9001:2008 Quality Recommissioning management system 4 Customer Care Manufacturing 3 © Praj Industries Ltd www.praj.net 3 Preface A by-product of sugar factory, is chiefly used as
    [Show full text]
  • Castillo, EF Et Al. Proc. Int. Soc. Sugar Cane Technol., Vol. 27, 2010
    Castillo, E.F. et al. Proc. Int. Soc. Sugar Cane Technol., Vol. 27, 2010 ______________________________________________________________________________________ THE COLOMBIAN EXPERIENCE IN THE PRODUCTION OF BIOETHANOL FOR TRANSPORT USE By E.F. CASTILLO, J.E. LARRAHONDO, A.L. GÓMEZ and J.I. SOCARRÁS Colombian Sugarcane Research Center, CENICAÑA [email protected] KEYWORDS: Sucro-Chemistry, Sugar Derivatives, Alcohol Industry, Vinasse, Bioethanol. Abstract THE NATIONAL Colombian Program for promoting the use of oxygenated gasoline for transport purposes started in 2001 with the implementation of the governmental tax incentives and the definition of the required technical framework for blending and fuel quality. The maximum level of blend for ethanol and gasoline was determined to be 10% v/v and the minimum concentration of dehydrated ethanol 99.5% v/v. Since 2005, five ethanol plants, using sugarcane feedstocks, are operating in the country with an overall production of 1 050 000 litres/day. The technological configuration of all plants is alike, mainly conformed by a continuous fermentation section and a continuous ethanol dehydration section. This paper describes some specific features derived from the commencement of the plants and some problems found in the normal operation of the fermentation. These troubles are mainly related to persistent microbial contamination in the fermentors, which reduces ethanol production efficiency and causes an overproduction of toxic and inhibitor organic compounds like acetic acid and lactic acid, among others. From a monitoring campaign, it was established that each plant had to develop its own adaptation process, such as the usage of different feedstock blending relationships, and to follow different learning curves despite the common technology used.
    [Show full text]
  • Biogas Production Potential from Native Beverage Vinasse of Mexico
    Waste Tech. Vol. 5(1)2017:9-14, Cruz-Salomón,Abumalé et al. Biogas Production Potential from Native Beverage Vinasse of Mexico Cruz-Salomón, Abumalé1, 2; Meza-Gordillo, Rocío2; Lagunas-Rivera, Selene3; Ventura-Canseco, Cristina2 1 Universidad de Ciencias y Artes de Chiapas. Facultad de Ingeniería. Libramiento Norte Poniente No. 1150. Col Lajas Maciel. Tuxtla Gutiérrez, Chiapas. C.P. 29000. 2Instituto Tecnológico de Tuxtla Gutiérrez. Carretera. Panamericana km. 1080, C.P. 29050.3Catedrática Conacyt, Instituto Tecnológico de México-Instituto Tecnológico de Tuxtla Gutiérrez. Carretera. Panamericana km. 1080, C.P. 29050. e-mail : [email protected] Abstract - This work presents the results of the physicochemical characterization and evaluates the potential of vinasse (a wastewater obtained from local beverage of Chiapas, Mexico) as nutrient source for biogas production by anaerobic digestion in an Upflow Anaerobic Sludge Blanket (UASB) bioreactor. This wastewater contains high level of organic matter, 120.2 ± 18.4 g O2/L of chemical organic demand (COD), 0.77 biodegradability index (BI), and a ratio 21:1 of C/N. The UASB bioreactor had a working volume of 4 L, at 32 oC, 10 days of hydraulic retention time (HRT). The COD removal was of 91 %, the production of methane was 1860 mL/day and 264.83 mL CH4/gCOD of biochemical methane potential (BMP).As such, the UASB bioreactor fed with vinasse can be sustainable alternative for the bioenergy production from wastewater, shifting the paradigm of wastewater management from ‘treatment and disposal’ to ‘beneficial utilization’ as well as ‘profitable endeavor’. Keywords – anaerobic digestion, UASB, vinasse,COD,biodegradability, BMP.
    [Show full text]
  • Techno-Economic Analysis of Vinasse Treatment Alternatives Through Process Simulation: a Case Study of Cuban Distillery
    Techno-Economic Analysis of Vinasse Treatment Alternatives Through Process Simulation: A Case Study of Cuban Distillery Arletis Cruz Llerena ICIDCA https://orcid.org/0000-0002-8113-1592 Osney Perez Ones ( [email protected] ) Universidad Tecnológica de La Habana José Antonio Echeverría: Universidad Tecnologica de La Habana Jose Antonio Echeverria https://orcid.org/0000-0002-0366-0317 Lourdes Zumalacárregui de Cárdenas Universidad Tecnológica de La Habana José Antonio Echeverría: Universidad Tecnologica de La Habana Jose Antonio Echeverria José Luis Pérez de los Ríos Ronera San José https://orcid.org/0000-0001-9442-3239 Research Article Keywords: sugarcane vinasse, treatment alternatives, Aspen Hysys, simulation, techno-economic analysis Posted Date: June 1st, 2021 DOI: https://doi.org/10.21203/rs.3.rs-518806/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/28 Abstract Purpose Vinasse is one of the organic industrial euents with major polluting effect. The objective of this work was to perform a techno-economic assessment of vinasses treatment alternatives for valorization of this waste through process simulation with Aspen Hysys v10.0. Methods Four alternatives were studied: (A_1) incineration and electricity generation, (A_2) desalinization, (A_3) anaerobic digestion and electricity generation and (A_4) drying. The selected packages for the evaluation and prediction of properties were: Lee-Kesler-Plöcker and NBS Steam, NRTL-Ideal, Peng-Robinson-Stryjer- Vera and NBS Steam and NRTL-Ideal respectively; the validation in these cases was carried out with data reported in the literature. The economic evaluation was carried according to the changes that each alternative determines in each one of the elements of effective cash ow comparing with the actual condition.
    [Show full text]
  • Condensed Molasses Solubles / Vinasses General Information (Product Code LQS Int2alle)
    Condensed Molasses Solubles / Vinasses General Information (Product code LQS INT2alle) General introduction Put simply, condensed molasses solubles (CMS) are by-products of various fermentation processes. Molasses is used as a nutrient substrate in many fermentation industries (e.g. yeast, alcohol and amino acid production and the production of organic acids). In these processes, most of the sucrose content of the molasses is consumed by microbiological action. The resulting liquid waste (known variously as slop, stillage, distiller’s wash or vinasse) has very little residual sugar content. It is this waste which is condensed, further processed and marketed as CMS. 1 Applications CMS is used primarily in feed products for cattle and sheep because its crude protein content consists mainly of NPN (nonprotein nitrogen compounds such as free amino acids and betaine). Only ruminants are capable of synthesizing NPN into protein. CMS is also of interest because of its mineral content, most notably potassium. In cattle feed, CMS is used in admixtures of about 3-4%. In pig and chicken feed, 1-2% is sufficient because in these cases it functions mainly as a binding agent rather than as a protein replacement. CMS can also be processed into value-added products by enrichment with other substances, such as proteins. CMS can be added to animal feed either directly or indirectly by mixing with another product, such as cane molasses. There are few if any palatability problems, and use as a feed for cattle, pigs and poultry should not cause any problems. CMS lends itself well to mixing with feed molasses because its low viscosity reduces the mixed viscosity of the resulting feed molasses product.
    [Show full text]
  • The Toxic Impact of Honey Adulteration: a Review
    foods Review The Toxic Impact of Honey Adulteration: A Review Rafieh Fakhlaei 1, Jinap Selamat 1,2,*, Alfi Khatib 3,4, Ahmad Faizal Abdull Razis 2,5 , Rashidah Sukor 2 , Syahida Ahmad 6 and Arman Amani Babadi 7 1 Food Safety and Food Integrity (FOSFI), Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; rafi[email protected] 2 Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; [email protected] (A.F.A.R.); [email protected] (R.S.) 3 Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan 25200, Pahang Darul Makmur, Malaysia; alfi[email protected] 4 Faculty of Pharmacy, Airlangga University, Surabaya 60155, Indonesia 5 Natural Medicines and Products Research Laboratory, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia 6 Department of Biochemistry, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; [email protected] 7 School of Energy and Power Engineering, Jiangsu University, Zhenjiang 212013, China; [email protected] * Correspondence: [email protected]; Tel.: +6-038-9769-1099 Received: 21 August 2020; Accepted: 11 September 2020; Published: 26 October 2020 Abstract: Honey is characterized as a natural and raw foodstuff that can be consumed not only as a sweetener but also as medicine due to its therapeutic impact on human health. It is prone to adulterants caused by humans that manipulate the quality of honey. Although honey consumption has remarkably increased in the last few years all around the world, the safety of honey is not assessed and monitored regularly.
    [Show full text]
  • Microbiological Colourants Removal from Sugar Beet Molasses Vinasse – the Effects of Process Parameters and Vinasse Dilution
    www.ees.uni.opole.pl ISSN paper version 1642-2597 ISSN electronic version 2081-8319 Economic and Environmental Studies Vol. 17, No. 2 (42/2017), 335-345, June 2017 Microbiological Colourants Removal from Sugar Beet Molasses Vinasse – The Effects of Process Parameters and Vinasse Dilution Marta WILK, Małgorzata KRZYWONOS and Przemysław SERUGA Wrocław University of Economics, Poland Abstract: Distilleries, in addition to ethanol, produced vinasse which is hazardous for the environment. Sugar beet molasses vinasse (BMV) is the most problematic waste from distilleries because of the coloured compounds contained therein. Traditional methods of the removal of the pollutant load from the waste do not allow simultaneous decolourization. The paper presents a microbiological method of coloured compounds removal from BMV. The conditions of the process (pH and temperature) and vinasse concentration were optimized. The bacteria Lactobacillus plantarum MiLAB393 applied showed the decolourization activity of 26% in medium consisted of 30% v/v of BMV at pH0=6.5 and 35.8°C. Keywords: decolourization, sugar beet molasses vinasse, Lactobacillus plantarum, lactic acid bacteria JEL codes: Q16, Q53, Q57 https://doi.org/10.25167/ees.2017.42.11 1. Introduction In the agricultural distilleries where the raw material used to produce ethanol is sugar beet molasses, in addition to the main product, sugar beet molasses vinasse is also formed. It is a by- -3 -3 product with a high load of pollutants (COD=55.5-147 g O2·dm , BOD5=27.5-69.3 g O2·dm ), acidity, pH=5.0 and dark brown color (Ryznar-Luty et al., 2009; Vlissidis, Zouboulis, 1993; Correspondence Address: Marta Wilk, Department of Bioprocess Engineering, Wrocław University of Economics, ul.
    [Show full text]
  • Sabnis Uta 2502D 12838.Pdf (5.069Mb)
    INVESTIGATION OF HOW MICROBES INVOLVED IN ANEROBIC DIGESTION OF VINASSE CHANGE AS FUNCTIONS OF TEMPERATURE, VINASSE COMPOSITIONS AND TIME by MADHU SANJOG SABNIS Presented to the Faculty of the Graduate School of The University of Texas at Arlington in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY THE UNIVERSITY OF TEXAS AT ARLINGTON August 2014 Copyright © by Madhu Sanjog Sabnis 2014 All Rights Reserved ii Acknowledgements I would like to express my wholehearted gratitude to my advisor Dr. Melanie Sattler and Dr. Jorge Rodrigues who has always been an invaluable guide and academic mentor. I really appreciate Dr. Melanie Sattler for her encouragement, support, and advice throughout my research work. I cannot express my gratitude to Dr. Rodrigues for providing excellent ideas and advice that propelled my research. The supervising committee members Dr. James Grover, Dr. Andrew Hunt, and Dr. Sahadat Hossain, are most appreciated and gratefully acknowledged for their valuable suggestion towards this study. A special and personal acknowledgement is due to the Dr. Melanie Sattler; for her unrestricted personal guidance throughout this study, for bringing out the best out of my ability. I would like to thank Mr. Paul Shover for his tremendous help with my experimental set-up work. I also thank Shammi Rahman for helping me throughout my research. I would like to thank Victor Pylro and Jill Castoe who helped me during my experimental analysis and sequencing process. Last but not the least; I thank God and my loving family - Mom, Dad, brother, sister, and my family-in-law. They are my real strength and have always believed in my abilities to not only dream but strive to make those dreams a concrete reality.
    [Show full text]
  • Sugarcane Molasses and Vinasse As a Substrate for Polyhydroxyalkanoates (PHA) Production
    Sugarcane molasses and vinasse as a substrate for • polyhydroxyalkanoates (PHA) production Alejandro Acosta-Cárdenas, Wilman Alcaraz-Zapata & Mariana Cardona-Betancur Research Group on Biotransformation, School of Microbiology, University of Antioquia, Medellin, Colombia, [email protected], [email protected], [email protected] Received: October 20th, de 2017. Received in revised form: July15th, 2018. Accepted: August 15th, 2018 Abstract Polyhydroxyalkanoates (PHA) are fully biodegradable biopolymers of microbial origin, with physicochemical characteristics similar to those of conventional synthetic plastics. However, their current production is highly expensive mainly due to the substrate or culture medium employed. In this research, a mixture of cane molasses and residual vinasse from the alcohol industry was evaluated as a substrate using the reference strain Ralstonia eutropha ATCC 17699 to produce PHAs. The results showed the potential of the mixture as a culture medium, after reaching polymer concentrations of 3.17 g/L in MSM medium and 2.71 g/L in M/V medium with a molasses/vinasse ratio of 25/75. There was a sugar (sucrose, fructose, and glucose) consumption yield of 70.7%, and a biopolymer accumulation of 97.8% with respect to the biomass produced. The biopolymer characterization showed a correlation with the standard polyhydroxybutyrate sample - PHB of 99.25%. Keywords: biopolymers; polyhydroxyalkanoates; Ralstonia; sugarcane molasses; sugarcane vinasse. Melazas y vinaza de la caña de azúcar como sustrato para la producción de polihidroxialcanoatos (PHA) Resumen Los Polihidroxialcanoatos (PHAs) son biopolímeros de origen microbiano totalmente biodegradables con características físicoquímicas similares a los plásticos sintéticos convencionales, sin embargo, su producción actual es altamente costosa debido principalmente al sustrato o medio de cultivo.
    [Show full text]