Ways of Ion Channel Gating in Plant Cells
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Glossary - Cellbiology
1 Glossary - Cellbiology Blotting: (Blot Analysis) Widely used biochemical technique for detecting the presence of specific macromolecules (proteins, mRNAs, or DNA sequences) in a mixture. A sample first is separated on an agarose or polyacrylamide gel usually under denaturing conditions; the separated components are transferred (blotting) to a nitrocellulose sheet, which is exposed to a radiolabeled molecule that specifically binds to the macromolecule of interest, and then subjected to autoradiography. Northern B.: mRNAs are detected with a complementary DNA; Southern B.: DNA restriction fragments are detected with complementary nucleotide sequences; Western B.: Proteins are detected by specific antibodies. Cell: The fundamental unit of living organisms. Cells are bounded by a lipid-containing plasma membrane, containing the central nucleus, and the cytoplasm. Cells are generally capable of independent reproduction. More complex cells like Eukaryotes have various compartments (organelles) where special tasks essential for the survival of the cell take place. Cytoplasm: Viscous contents of a cell that are contained within the plasma membrane but, in eukaryotic cells, outside the nucleus. The part of the cytoplasm not contained in any organelle is called the Cytosol. Cytoskeleton: (Gk. ) Three dimensional network of fibrous elements, allowing precisely regulated movements of cell parts, transport organelles, and help to maintain a cell’s shape. • Actin filament: (Microfilaments) Ubiquitous eukaryotic cytoskeletal proteins (one end is attached to the cell-cortex) of two “twisted“ actin monomers; are important in the structural support and movement of cells. Each actin filament (F-actin) consists of two strands of globular subunits (G-Actin) wrapped around each other to form a polarized unit (high ionic cytoplasm lead to the formation of AF, whereas low ion-concentration disassembles AF). -
Biopsychology 2012 – Sec 003 (Dr
Biopsychology 2012 – sec 003 (Dr. Campeau) Study Guide for First Midterm What are some fun facts about the human brain? - there are approximately 100 billion neurons in the brain; - each neuron makes between 1000 to 10000 connections with other neurons; - speed of action potentials varies from less than 1 mph and up to 100 mph. What is a neuron? A very specialized cell type whose function is to receive, process, and send information; these cells are found in the central nervous system (CNS – brain, spinal cord, retina) and the peripheral nervous system (PNS – the rest of the body). What is a nerve? They are axons of individual neurons in bundles or strands of many axons. What are the major parts of a neuron? - cell membrane: “skin” of the neuron; - cytoplasm: everything inside the skin; - nucleus: contains chromosomes (DNA); - ribosomes: generate proteins from mRNA; - mitochondria: energy “generator” of cells (produce ATP); - mitochondria: moves “stuff” inside the neuron (like a tow rope); - soma: cell body, excluding dendrites and axons; - dendrites and spines: part of the neuron usually receiving information from other neurons; - axons: part of the neuron that transmits information to other neurons; - myelin sheath: surrounds axons and provides electrical “insulation”; - nodes of Ranvier: small area on axons devoid of myelin sheath; - presynaptic terminal: area of neuron where neurotransmitter is stored and released by action potentials. What are the different neuron types according to function? 1. Sensory neurons: neurons specialized to “receive” information about the environment. 2. Motor neurons: neurons specialized to produce movement (contraction of muscles). 3. Interneurons or Intrinsic neurons: neurons, usually with short axons, that handle local information. -
Interplay Between Gating and Block of Ligand-Gated Ion Channels
brain sciences Review Interplay between Gating and Block of Ligand-Gated Ion Channels Matthew B. Phillips 1,2, Aparna Nigam 1 and Jon W. Johnson 1,2,* 1 Department of Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260, USA; [email protected] (M.B.P.); [email protected] (A.N.) 2 Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA 15260, USA * Correspondence: [email protected]; Tel.: +1-(412)-624-4295 Received: 27 October 2020; Accepted: 26 November 2020; Published: 1 December 2020 Abstract: Drugs that inhibit ion channel function by binding in the channel and preventing current flow, known as channel blockers, can be used as powerful tools for analysis of channel properties. Channel blockers are used to probe both the sophisticated structure and basic biophysical properties of ion channels. Gating, the mechanism that controls the opening and closing of ion channels, can be profoundly influenced by channel blocking drugs. Channel block and gating are reciprocally connected; gating controls access of channel blockers to their binding sites, and channel-blocking drugs can have profound and diverse effects on the rates of gating transitions and on the stability of channel open and closed states. This review synthesizes knowledge of the inherent intertwining of block and gating of excitatory ligand-gated ion channels, with a focus on the utility of channel blockers as analytic probes of ionotropic glutamate receptor channel function. Keywords: ligand-gated ion channel; channel block; channel gating; nicotinic acetylcholine receptor; ionotropic glutamate receptor; AMPA receptor; kainate receptor; NMDA receptor 1. Introduction Neuronal information processing depends on the distribution and properties of the ion channels found in neuronal membranes. -
Nernst Potentials and Membrane Potential Changes
UNDERSTANDING MEMBRANE POTENTIAL CHANGES IN TERMS OF NERNST POTENTIALS: For seeing how a change in conductance to ions affects the membrane potential, follow these steps: 1. Make a graph with membrane potential on the vertical axis (-100 to +55) and time on the horizontal axis. 2. Draw dashed lines indicating the standard Nernst potential (equilibrium potential) for each ion: Na+ = +55 mV, K+ = -90mV, Cl- = -65 mV. 3. Draw lines below the horizontal axis showing the increased conductance to individual ions. 4. Start plotting the membrane potential on the left. Most graphs will start at resting potential (-70 mV) 5. When current injection (Stim) is present, move the membrane potential upward to Firing threshold. 6. For the time during which membrane conductance to a particular ion increases, move the membrane potential toward the Nernst potential for that ion. 7. During the time when conductance to a particular ion decreases, move the membrane potential away from the Nernst potential of that ion, toward a position which averages the conductances of the other ions. 8. When conductances return to their original value, membrane potential will go to its starting value. +55mV ACTION POTENTIAL SYNAPTIC POTENTIALS 0mV Membrane potential Firing threshold Firing threshold -65mV -90mV Time Time Na+ K+ Conductances Stim CHANGES IN MEMBRANE POTENTIAL ALLOW NEURONS TO COMMUNICATE The membrane potential of a neuron can be measured with an intracellular electrode. This 1 provides a measurement of the voltage difference between the inside of the cell and the outside. When there is no external input, the membrane potential will usually remain at a value called the resting potential. -
Neurotransmitter Actions
Central University of South Bihar Panchanpur, Gaya, India E-Learning Resources Department of Biotechnology NB: These materials are taken/borrowed/modified/compiled from various resources like research articles and freely available internet websites, and are meant to be used solely for the teaching purpose in a public university, and for serving the needs of specified educational programmes. Dr. Jawaid Ahsan Assistant Professor Department of Biotechnology Central University of South Bihar (CUSB) Course Code: MSBTN2003E04 Course Name: Neuroscience Neurotransmitter Actions • Excitatory Action: – A neurotransmitter that puts a neuron closer to an action potential (facilitation) or causes an action potential • Inhibitory Action: – A neurotransmitter that moves a neuron further away from an action potential • Response of neuron: – Responds according to the sum of all the neurotransmitters received at one time Neurotransmitters • Acetylcholine • Monoamines – modified amino acids • Amino acids • Neuropeptides- short chains of amino acids • Depression: – Caused by the imbalances of neurotransmitters • Many drugs imitate neurotransmitters – Ex: Prozac, zoloft, alcohol, drugs, tobacco Release of Neurotransmitters • When an action potential reaches the end of an axon, Ca+ channels in the neuron open • Causes Ca+ to rush in – Cause the synaptic vesicles to fuse with the cell membrane – Release the neurotransmitters into the synaptic cleft • After binding, neurotransmitters will either: – Be destroyed in the synaptic cleft OR – Taken back in to surrounding neurons (reuptake) Excitable cells: Definition: Refers to the ability of some cells to be electrically excited resulting in the generation of action potentials. Neurons, muscle cells (skeletal, cardiac, and smooth), and some endocrine cells (e.g., insulin- releasing pancreatic β cells) are excitable cells. -
Effect of Hyperkalemia on Membrane Potential: Depolarization
❖ CASE 3 A 6-year-old boy is brought to the family physician after his parents noticed that he had difficulty moving his arms and legs after a soccer game. About 10 minutes after leaving the field, the boy became so weak that he could not stand for about 30 minutes. Questioning revealed that he had complained of weakness after eating bananas, had frequent muscle spasms, and occasionally had myotonia, which was expressed as difficulty in releasing his grip or diffi- culty opening his eyes after squinting into the sun. After a thorough physical examination, the boy was diagnosed with hyperkalemic periodic paralysis. The family was advised to feed the boy carbohydrate-rich, low-potassium foods, give him glucose-containing drinks during attacks, and have him avoid strenuous exercise and fasting. ◆ What is the effect of hyperkalemia on cell membrane potential? ◆ What is responsible for the repolarizing phase of an action potential? ◆ What is the effect of prolonged depolarization on the skeletal muscle Na+ channel? 32 CASE FILES: PHYSIOLOGY ANSWERS TO CASE 3: ACTION POTENTIAL Summary: A 6-year-old boy who experiences profound weakness after exer- cise is diagnosed with hyperkalemic periodic paralysis. ◆ Effect of hyperkalemia on membrane potential: Depolarization. ◆ Repolarization mechanisms: Activation of voltage-gated K+ conductance and inactivation of Na+ conductance. ◆ Effect of prolonged depolarization: Inactivation of Na+ channels. CLINICAL CORRELATION Hyperkalemic periodic paralysis (HyperPP) is a dominant inherited trait caused by a mutation in the α subunit of the skeletal muscle Na+ channel. It occurs in approximately 1 in 100,000 people and is more common and more severe in males. -
Nicotinic Acetylcholine Receptor Signaling in Neuroprotection
Akinori Akaike · Shun Shimohama Yoshimi Misu Editors Nicotinic Acetylcholine Receptor Signaling in Neuroprotection Nicotinic Acetylcholine Receptor Signaling in Neuroprotection Akinori Akaike • Shun Shimohama Yoshimi Misu Editors Nicotinic Acetylcholine Receptor Signaling in Neuroprotection Editors Akinori Akaike Shun Shimohama Department of Pharmacology, Graduate Department of Neurology, School of School of Pharmaceutical Sciences Medicine Kyoto University Sapporo Medical University Kyoto, Japan Sapporo, Hokkaido, Japan Wakayama Medical University Wakayama, Japan Yoshimi Misu Graduate School of Medicine Yokohama City University Yokohama, Kanagawa, Japan ISBN 978-981-10-8487-4 ISBN 978-981-10-8488-1 (eBook) https://doi.org/10.1007/978-981-10-8488-1 Library of Congress Control Number: 2018936753 © The Editor(s) (if applicable) and The Author(s) 2018. This book is an open access publication. Open Access This book is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made. The images or other third party material in this book are included in the book’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the book’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The use of general descriptive names, registered names, trademarks, service marks, etc. -
Information Processing in the Axon Dominique Debanne
Information processing in the axon Dominique Debanne To cite this version: Dominique Debanne. Information processing in the axon. Nature Reviews Neuroscience, Nature Publishing Group, 2004, 5 (4), pp.304-316. 10.1038/nrn1397. hal-01766862 HAL Id: hal-01766862 https://hal-amu.archives-ouvertes.fr/hal-01766862 Submitted on 27 Aug 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. INFORMATION PROCESSING IN THE AXON Dominique Debanne Axons link distant brain regions and are generally regarded as reliable transmission cables in which stable propagation occurs once an action potential has been generated. However, recent experimental and theoretical data indicate that the functional capabilities of axons are much more diverse than traditionally thought. Beyond axonal propagation, intrinsic voltage-gated conductances together with the intrinsic geometrical properties of the axon determine complex phenomena such as branch-point failures and reflected propagation. This review considers recent evidence for the role of these forms of axonal computation in the short-term dynamics of neural communication. GAP JUNCTIONS Since the pioneering work of Santiago Ramón y Cajal, of propagation and how intrinsic channels that are Morphological equivalent of the axon has been defined as a long neuronal process present in the axon shape the action potential. -
Electrical Activity of the Heart: Action Potential, Automaticity, and Conduction 1 & 2 Clive M
Electrical Activity of the Heart: Action Potential, Automaticity, and Conduction 1 & 2 Clive M. Baumgarten, Ph.D. OBJECTIVES: 1. Describe the basic characteristics of cardiac electrical activity and the spread of the action potential through the heart 2. Compare the characteristics of action potentials in different parts of the heart 3. Describe how serum K modulates resting potential 4. Describe the ionic basis for the cardiac action potential and changes in ion currents during each phase of the action potential 5. Identify differences in electrical activity across the tissues of the heart 6. Describe the basis for normal automaticity 7. Describe the basis for excitability 8. Describe the basis for conduction of the cardiac action potential 9. Describe how the responsiveness relationship and the Na+ channel cycle modulate cardiac electrical activity I. BASIC ELECTROPHYSIOLOGIC CHARACTERISTICS OF CARDIAC MUSCLE A. Electrical activity is myogenic, i.e., it originates in the heart. The heart is an electrical syncitium (i.e., behaves as if one cell). The action potential spreads from cell-to-cell initiating contraction. Cardiac electrical activity is modulated by the autonomic nervous system. B. Cardiac cells are electrically coupled by low resistance conducting pathways gap junctions located at the intercalated disc, at the ends of cells, and at nexus, points of side-to-side contact. The low resistance pathways (wide channels) are formed by connexins. Connexins permit the flow of current and the spread of the action potential from cell-to-cell. C. Action potentials are much longer in duration in cardiac muscle (up to 400 msec) than in nerve or skeletal muscle (~5 msec). -
The Excitable Cell. Resting Membrane Potential and Action Potential (1&2)
The excitable cell. Resting Membrane Potential and Action Potential (1&2) Dr Sergey Kasparov School of Medical Sciences, Room E9 THIS TOPIC IS ALSO COVERED IN TUTORIALS! Teaching home page: http://www.bristol.ac.uk/phys-pharm/media/teaching/ Why do we call these cells “excitable”? Real action potentials.avi The key point: Bioelectricity is generated by the ions moving across cellular membranes Concentration of selected solutes in intracellular fluid and extracellular fluid in millimols mM 160 140 120 100 Insi de the cell 80 60 In extracellular fluid 40 20 0 1 The Na+/K+ pump outside inside [[mMmM]] + + [[mMmM]] + + + + + + + Na 145 + + 15 + K 4 + + 140 ATPATP ••isis an active ion transporter (ATP‐dependent) ••isis responsible for creating and upholding Na+ and K+ concentration gradients ••isis electrogenic – pumps 3 Na+ versus 2 K+ ions Reminder: Movement of ions is affected by concentration gradient and the electrical field Chemical driving force _ _ + + _ + + Electrical driving force _ + + _ + + + _+ _ + _ + + + + _ + _ + _ + + _ Net flux + + + + _ + _ + + V REMINDER: Diffusion through Ion Channels A leak channel A gated channel Both leak and gated channels allow movement of molecules (mainly inorganic ions) down the electrochemical gradient. So, if the gradient reverses, the ions will flow in the opposite direction. 1. The channels are aqueous pores through the membrane. 2. The channels are usually quite selective, for example some only pass Na+, others K+, still others – Cl- 3. Gated channels may be opened or closed by various factors -
9.01 Introduction to Neuroscience Fall 2007
MIT OpenCourseWare http://ocw.mit.edu 9.01 Introduction to Neuroscience Fall 2007 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. 9.01 Recitation (R02) RECITATION #2: Tuesday, September 18th Review of Lectures: 3, 4 Reading: Chapters 3, 4 or Neuroscience: Exploring the Brain (3rd edition) Outline of Recitation: I. Previous Recitation: a. Questions on practice exam questions from last recitation? II. Review of Material: a. Exploiting Axoplasmic Transport b. Types of Glia c. THE RESTING MEMBRANE POTENTIAL d. THE ACTION POTENTIAL III. Practice Exam Questions IV. Questions on Pset? Exploiting Axoplasmic Transport: Maps connections of the brain Rates of transport: - slow: - fast: Examples: Uses anterograde transport: - Uses retrograde transport: - - - Types of Glia: - Microglia: - Astrocytes: - Myelinating Glia: 1 THE RESTING MEMBRANE POTENTIAL: The Cast of Chemicals: The Movement of Ions: Influences by two factors: (1) Diffusion: (2) Electricity: Ohm’s Law: I = gV Ionic Equilibrium Potentials (EION): + Example: ENs * diffusional and electrical forces are equal 2 Nernst Equation: EION = 2.303 RT/zF log [ion]0/[ion]i Calculates equilibrium potential for a SINGLE ion. Inside Outside EION (at 37°C) + [K ] + [Na ] 2+ [Ca ] [Cl ] *Pumps maintain concentration gradients (ex. sodiumpotassium pump; calcium pump) Resting Membrane Potential (VM at rest): Measured resting membrane potential: 65 mV Goldman Equation: + + + + VM = 61.54 mV log (PK[K ]o + PNa[Na ]o)/ (PK[K ]I + PNa[Na ]i) + + Calculates membrane potential when permeable to both Na and K . Remember: at REST, gK >>> gNa therefore, VM is closer to EK THE ACTION POTENTIAL (Nerve Impulse): Phases of an Action Potential: Vm (mV) ENa 0 EK Time 3 Conductance of Ion Channels during AP: Remember: Changes in conductance, or permeability of the membrane to a specific ion, changes the membrane potential. -
11 Introduction to the Nervous System and Nervous Tissue
11 Introduction to the Nervous System and Nervous Tissue ou can’t turn on the television or radio, much less go online, without seeing some- 11.1 Overview of the Nervous thing to remind you of the nervous system. From advertisements for medications System 381 Yto treat depression and other psychiatric conditions to stories about celebrities and 11.2 Nervous Tissue 384 their battles with illegal drugs, information about the nervous system is everywhere in 11.3 Electrophysiology our popular culture. And there is good reason for this—the nervous system controls our of Neurons 393 perception and experience of the world. In addition, it directs voluntary movement, and 11.4 Neuronal Synapses 406 is the seat of our consciousness, personality, and learning and memory. Along with the 11.5 Neurotransmitters 413 endocrine system, the nervous system regulates many aspects of homeostasis, including 11.6 Functional Groups respiratory rate, blood pressure, body temperature, the sleep/wake cycle, and blood pH. of Neurons 417 In this chapter we introduce the multitasking nervous system and its basic functions and divisions. We then examine the structure and physiology of the main tissue of the nervous system: nervous tissue. As you read, notice that many of the same principles you discovered in the muscle tissue chapter (see Chapter 10) apply here as well. MODULE 11.1 Overview of the Nervous System Learning Outcomes 1. Describe the major functions of the nervous system. 2. Describe the structures and basic functions of each organ of the central and peripheral nervous systems. 3. Explain the major differences between the two functional divisions of the peripheral nervous system.