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A Licence to Kill During Inflammation

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A Licence to Kill During Inflammation RESEARCH NEWS & VIEWS of these compounds, the results confirmed to use this approach to make non­natural Monica E. McCallum and Emily P. Balskus that the addition of multiple sulfo groups to PST analogues for evaluation as candidate are in the Department of Chemistry and PSTs reduces the compounds’ binding affini­ therapeutics. Chemical Biology, Harvard University, ties to voltage­gated sodium channels. This However, substantial barriers must be Cambridge, Massachusetts 02138, USA. strongly suggests that sulfo groups reduce PST surmounted before these sulfotransferase e-mail: [email protected] toxi city, further highlighting their potential for enzymes can be fully integrated into PST syn­ 1. Durán-Riveroll, L. M. & Cembella, A. D. Mar. Drugs incorporation into PST­based drug candidates. theses. Their catalytic efficiency is very low, 15, 303 (2017). The use of biosynthetic enzymes to modify and they have not yet been used on a large 2. Lukowski, A. L. et al. ACS Chem. Biol. 14, 941–948 PSTs represents a strategy that is distinct from scale — Lukowski (2019). the chemical­synthesis approaches more and colleagues 3. van Apeldoorn, M. E., van Egmond, H. P., frequently used to make analogues of these “These worked at a sub­ Speijers, G. J. A. & Bakker, G. J. I. Mol. Nutr. Food Res. 12 51, 7–60 (2007). natural products . Although many of those findings offer milligram scale, but 4. Bustillos-Guzmán, J. J. et al. Food Addit. Contam. A synthetic efforts have been successful, they researchers the multi­gram quanti­ 32, 381–394 (2015). often involve long sequences of reactions opportunity ties of PST analogues 5. Andrinolo, D., Michea, L. F. & Lagos, N. Toxicon 37, and deliver low yields of products as a con­ to combine would eventually be 447−464 (1999). 6. Andrinolo, D., Iglesias, V., Garcia, C. & Lagos, N. sequence of the challenging architectures of conventional needed for the pre­ Toxicon 40, 699−709 (2002). the PSTs — which contain an abundance of synthetic clinical development 7. Sako, Y. et al. J. Phycol. 37, 1044−1051 (2001). reactive oxygen and nitrogen atoms that com­ chemistry with of drug candi dates. 8. Yoshida, T. et al. Fish. Sci. 68, 634−642 (2002). plicate the use of more­standard chemical Also, the reactivity of 9. Wang, D.-Z., Zhang, S.-F., Zhang, Y. & Lin, L. biocatalysis.” J. Proteom. 135, 132–140 (2016). reactions. Lukowski and colleagues’ find­ the enzymes towards 10. Cullen, A. et al. ACS Chem. Biol. 13, 3107−3114 ings now offer researchers the opportunity non­natural PST (2018). to combine conventional synthetic chemistry scaffolds, or towards members of related toxin 11. Lukowski, A. L. et al. J. Am. Chem. Soc. 140, with biocatalysis, using enzymes to further families, has yet to be evaluated. If the reactiv­ 11863–11869 (2018). modify PST scaffolds obtained by synthetic ity and selectivity of the sulfotransferases can 12. Berlinck, R. G. S., Bertonha, A. F., Takaki, M. & Rodriguez, J. P. G. Nat. Prod. Rep. 34, 1264–1301 routes. This could potentially streamline be optimized using enzyme engineering, these (2017). access to sulfated versions of these natural biocatalysts will become powerful synthetic products. It might eventually even be possible tools in the search for new pain therapeutics. ■ This article was published online on 10 June 2019. IMMUNOLOGY It is widely accepted that the tightly regulated formation of NLRP3­containing inflammasomes occurs in two steps. In the first step, NLRP3 is primed for action by other A licence to kill during immune­sensor proteins called TLRs, which can detect components of microorganisms. This priming step occurs in two ways2: NLRP3 inflammation can undergo a modification, such as the addi­ tion of a phosphate group or the removal of an Inflammasomes are protein complexes that fight infection by driving inflammation attached ubiquitin protein. Further priming or cell death. It now seems that the protein NEK7 provides a ‘licence’ for the is achieved by a rise in expression of the gene formation of inflammasomes containing the protein NLRP3. See Article p.338 that encodes NLRP3, increasing the chance that NLRP3 will detect any abnormalities. The second step, activation, then results in NLRP3 KENGO NOZAKI & EDWARD A. MIAO function downstream of an inflammasome proteins binding together to form part of a can potently drive both inflammation and cell disc­shaped inflammasome complex that is nflammation can help to eliminate death2. Different types of inflammasome can probably similar to those of other inflamma­ infection, but excessive inflammation can form depending on the sensor components somes containing proteins of the NLR family cause damage to the body. The sensor pro­ involved. Certain inflammasomes respond to (which includes NLRP3 and NLRC4)5,6. This Iteins that trigger an inflammatory immune a highly specific trigger: for example, those in activation step occurs during a cellular cata­ response must therefore be carefully regulated. mammalian cells containing the sensor pro­ strophe, but the biochemical and structural Some intracellular immune­sensor proteins tein NLRC4 respond to the presence of the mechanisms involved are unknown. detect components in a cell that become abnor­ bacterial protein flagellin3,4. Researchers have long sought to determine mal or altered during a cellular crisis. Signs of Proteins that are normally present in the structure of NLRP3 as it forms an inflam­ cellular crisis are sometimes produced in the mammalian cells do not seem able to trigger masome, in the hope of gaining insights into absence of an infection, so mechanisms are the accidental formation of NLRC4­containing how this protein functions. However, such needed to prevent the proteins from trigger­ inflammasomes, given the lack of reports of such efforts have been unsuccessful, perhaps because ing an inappropriate inflammatory response. aberrant events. By contrast, inflamma somes unknown protein partners that interact with Sharif et al.1 report a structural study on that contain NLRP3 are activated when NLRP3 NLRP3 were missing from earlier attempts. page 338 that investigates an immune­sensor recognizes — by an as yet unknown mecha­ The discovery7–9 that the enzyme NEK7 protein called NLRP3, revealing that a protein nism — hallmarks of cellular catastrophe, such is essential for NLRP3 signalling provided a called NEK7 acts as a ‘licence’ that enables this as extremely low concentrations of potassium missing part of the puzzle. NEK7 regulates pro­ protein to cause inflammation. in the cytoplasm, or signs of dysfunction in cesses that occur during cell division, such as the When an immune sensor recognizes organelles called mitochondria2. Such events breakdown of the nuclear­envelope structure10, a hallmark of infection in the cytoplasm, can arise from tissue damage that is un related so it was surprising to find that it has a separate this can activate the protein and lead to the to infection, and NLRP3 activation in such role in inflammation. This suggested that assembly of a multiprotein complex called an cases has been implicated as a possible cause of NLRP3­containing inflammasome formation inflamma some. The activation of proteins that inflammatory diseases such as atherosclerosis. doesn’t occur during cell division because NEK7 316 | NATURE | VOL 570 | 20 JUNE 2019 ©2019 Spri nger Nature Li mited. All ri ghts reserved. ©2019 Spri nger Nature Li mited. All ri ghts reserved. NEWS & VIEWS RESEARCH NACHT domain of NLRP3 P WHD HD1 NBD HD2 NEK7 Ubiquitin LRR domain Licensing Priming Activation Oligomerization of NLRP3 Licence revoked during cell division Inammasome formation causes inammation or cell death Figure 1 | The NEK7 protein enables the formation of a multiprotein NLRP3’s LRR domain and to its NACHT domain in the HD2 subdomain. An immune-defence complex called the inflammasome. Sharif et al.1 report early step leading to inflammasome formation is called priming, which is when structural studies of the assembly of an inflammasome containing the human NLRP3 either gains a phosphate group (P) or loses an attached ubiquitin protein2. protein NLRP3. Inflammasome formation requires7–9 the enzyme NEK7. This is followed by rotational activation of the NACHT domain, which exposes Sharif and colleagues report that NEK7 helps the inflammasome to assemble by all four of its subdomains. By analogy with another type of inflammasome5,6, providing a ‘licence’ for its formation. Given that NEK7 has roles in cell division, three of these subdomains (NBD, HD1 and WHD) might form interactions this licensing activity is probably revoked if NEK7 is not available when cells (red arrows) between adjacent proteins. Sharif et al. report that NEK7 forms a divide. NLRP3 has a NACHT domain composed of the subdomains: NBD, connection (green arrow) between LRR domains of adjacent NLRP3 proteins HD1, WHD and HD2 (the WHD subdomain is exposed when the protein is as the oligomerization of proteins occurs during inflammasome formation. The activated) and an LRR domain. In the inactive state of NLRP3, NEK7 binds to assembled inflammasome can cause inflammation or cell death. is unavailable to aid inflammasome assembly8. adjacent NLRP3 in the inflammasome. The perhaps the most important question of all: NEK7 regulates NLRP3 signalling by binding ability to form such an interface suggests that what direct interaction between NLRP3 and to a region of the protein known as the LRR NEK7 provides a licence for NLRP3 to form an unknown cellular factor results in the domain8,9. However, why
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