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Applied Biosystems 7500 Real-Time PCR System and Applied Biosystems 7500 Fast Real-Time PCR System a Real Fast and Real Versatile Approach to Real-Time PCR

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58374r1_AB04499 12/15/04 10:50 PM Page 1 Specification Sheet Real-Time PCR Systems Applied Biosystems 7500 Real-Time PCR System and Applied Biosystems 7500 Fast Real-Time PCR System A Real Fast and Real Versatile Approach to Real-Time PCR • The 7500 Fast Real-Time PCR System enables standard 96-well format high speed thermal cycling, significantly reducing your run time for quantitative real-time PCR applications, deliv- ering results in about 35 minutes Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR System • The 7500 Real-Time PCR System is a versatile, leading-edge platform providing enhanced performance •Small instrument footprint permits Fast Thermal Cycling capabilities and an upgrade path easy placement in any laboratory, The 7500 System is available in two to high-speed thermal cycling even those with limited space configurations; the 7500 Fast System configuration with a high-speed, 96- •Five-color detection system provides Introduction well format thermal cycling block the flexibility to perform a variety The Applied Biosystems 7500 and capable of quantitative real-time PCR of applications including gene 7500 Fast Real-Time PCR Systems are runs in about 35 minutes, and the expression analysis, SNP genotyp- integrated, versatile platforms for the standard configuration with a 96-well ing and plus/minus assays that detection and quantification of nucleic format thermal cycling block with utilize internal positive controls acid sequences. Real-time PCR com- run times of under 2 hours. A service bines thermal cycling, fluorescence •Advanced optical configuration engineer installed Fast Upgrade Kit detection, and application-specific supports a broader range of fluo- is available for the 7500 System to software to measure the cycle-by-cycle rophores, including FAM™/SYBR® upgrade it to the Fast configuration. accumulation of PCR products in a Green I, VIC®/JOE™, NED™/ single-tube homogenous reaction. The Applied Biosystems 7500 Fast TAMRA™/Cy3™, ROX™/Texas Red®, Real-Time PCR System delivers high- and Cy5™ dyes; variable excitation Quantitative results are available speed cycling capabilities with a new capability allows greater sensitivity immediately upon completion of fast ramping Peltier-based thermal for longer wavelength (red) dyes PCR, with no need to run gels, purify cycling block. This hardware, together PCR products, or perform any post- •Powerful, flexible software with new Optical Fast 96-well reaction PCR manipulation. Compared with includes new plate setup wizards plates and a new hot-start DNA manual PCR quantitation techniques, to guide you through experimental polymerase containing master mix, such as Northern blotting or RNase setup, while automated analysis comprise the complete Fast system. protection assays, real-time PCR offers tools such as RQ Study (Relative Using this system quantitative real- an enormous time savings, greater sen- Quantitation) gene expression time PCR runs can be completed in sitivity, superior precision, and a larger analysis software allow the simul- about 35 minutes with comparable dynamic range. taneous analysis and visualization performance to standard run times of up to ten 96-well plates of data for supported applications. www.appliedbiosystems.com 58374r1_AB04499 12/15/04 10:50 PM Page 2 The 7500 Fast System supports three Demonstrated Performance Specifications thermal cycling modes; Fast, Standard Instrument 7500 System 7500 Fast System and 9600 emulation. Standard and 9600 emulation thermal cycling modes Dynamic Range 9 logs of linear dynamic range 9 logs of linear dynamic range on the 7500 Fast System have ramp Sensitivity Detection of 10 copies of Detection of 10 copies of rates comparable to the standard 7500 template in a 50 µL reaction for template in a 20 µL reaction for ® ® System. Data collection processes for a single reporter TaqMan assay, a single reporter TaqMan assay, with 99.7% confidence with 99.7% confidence Fast 7500 Systems remain the same as for standard 7500 Systems. Run Time 1 hour and 50 minute run time 37 minute run time for a 40 cycle for a 40 cycle PCR reaction PCR reaction using the TaqMan® Real-Time PCR Applications using the TaqMan® RNase P RNase P Fast Instrument The 7500 and 7500 Fast Systems sup- Instrument Verification Plate Verification Plate port many real-time PCR applications, including gene expression analysis using relative quantitation (RQ) assays Sequence Detection Software • Automated SNP genotype calling and pathogen detection using standard Sequence Detection Software for the capability, including 2-cluster calling, curves. In addition, the systems allow 7500 and 7500 Fast systems runs on with intuitive graphical output and for qualitative post-PCR detection of the Windows XP® operating system quality-value assignment, makes SNP nucleic acids for allelic discrimination and is used for instrument control, analysis simple and straightforward (SNP genotyping assays) and data collection, and data analysis. • Simple dissociation curve data col- plus/minus assays that utilize internal Powerful and user-friendly, the soft- lection and viewing, enabling the positive controls. ware includes: addition of dissociation stages while runs are in progress Fluorescence Detection • Plate set-up wizards for easy experi- • Tool tips for easy identification of All sample wells are illuminated with a mental design, even with complex sample wells when viewing amplifica- tungsten halogen lamp. Light from this multicolor assays tion curve or SNP genotyping plots lamp passes through five excitation filters • The added ability for the 7500 Fast • Lamp-life monitoring and instrument before reaching sample wells. The System to choose between Fast, diagnostics to provide confidence in inclusion of excitation filters improves Standard, and 9600 emulation your instrument performance the ability of the instrument to excite thermal cycling modes dyes at longer (red) wavelengths, • Real-time monitoring of amplifica- Installation Specifications resulting in greater sensitivity and pre- tion growth curves enabling viewing The TaqMan® Fast RNase P cision for these dyes. Fluorescence of runs in progress Instrument Verification Plate enables emission is then detected through five • The flexibility to add extra PCR the 7500 Fast System to distinguish emission filters (even in Fast thermal cycles while runs are in progress between samples containing 5,000 and cycling mode) to a charge-coupled • Auto-baseline and auto-threshold 10,000 template DNA copies, with a device (CCD) camera. Emission filters for simplified data analysis statistical confidence level of 99.7% are optimized for use with FAM™/ • Absolute quantitation of nucleic acid and a run time of about 35 minutes. SYBR® Green I, VIC®/JOE™, targets with the ability to simulta- The TaqMan® Fast RNase P NED™/TAMRA™/Cy3™, ROX™/Texas neously analyze multiple standard Instrument Verification Plate enables Red®, and Cy5™ fluorescent dyes. curves on a single plate, improving the 7500 System to distinguish your workflow and easing the between samples containing 5,000 and burden of downstream analysis 10,000 template DNA copies, with a • RQ (Relative Quantitation) gene statistical confidence level of 99.7%. expression analysis software with powerful data viewing capabilities allowing the simultaneous analysis of up to ten 96-well plates of data www.appliedbiosystems.com 58374r1_AB04499 12/15/04 10:50 PM Page 3 Computer Specifications Instrument Specifications Applied Biosystems supplies a Dell™ Instrument 7500 System and 7500 Fast System business line computer (note book or tower) for use with the 7500 and Thermal cycling system Peltier-based, 96-well block 7500 Fast Systems. For the latest Optical system Five-excitation, Five-emission filters and CCD camera computer specifications please visit Instrument 7500 System 7500 Fast System the Applied Biosystems website at www.appliedbioystems.com Quantitative PCR run time < 2 hours < 40 minutes Reagents and Disposables Supported A complete line of reagents including Volumes 20-100 µL 10–30 µL TaqMan® Fast Universal PCR Master Supported • Standard Optical 96-well plates • Optical Fast 96-well plates ® Mix, TaqMan Universal Master Consumables • 8-strip 0.2mL tubes • Optical Adhesive Covers Mixes, SYBR® Green I Master Mixes, • 0.2mL tubes and disposables including 96-well • Optical Adhesive Covers • Optical Flat Caps plates, is available for use with the Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems. Supported Applications Service and Warranty Instrument 7500 System 7500 Fast System Purchase of the 7500 System or 7500 Standard/9600 Standard/9600 Fast System includes a one-year lim- Thermal Cycling Mode Emulation Fast Emulation* ited warranty on parts and labor. Purchase of the 7500 Fast System Absolute Quantitation Yes Yes Yes Upgrade Kit includes a 90-day limited Relative Quantitation Yes Yes Yes warranty on parts and labor. The 7500 Allelic Discrimination Yes No Yes System, 7500 Fast System and 7500 Fast Upgrade Kit include set-up and Plus/Minus Assays using IPC Yes No Yes calibration of the instrument by our *Ramp rates for Standard/9600 emulation mode are matched to the 7500 System Standard/9600 emulation highly trained Service Support team. modes. However, overall run times are shorter on the 7500 Fast System for these modes. Support Technical specialists and scientists TaqMan® Genomic Assay Products provide worldwide applications support Applied Biosystems provides preformulated, ready-to-use, quality-tested, and service. 5' nuclease TaqMan® assays for use with the 7500 and 7500 Fast systems. Assay Details Application Assay Formats Gene Expression SNP Genotyping TaqMan® Genomic Assays Yes Yes Custom TaqMan® Genomic Assays Yes Yes Genome Availability Number of Assays Human > 204,000 > 1,990,000 Mouse > 179,000N/A Rat > 128,000N/A URL (assays can be ordered online only) www.allgenes.com www.allsnps.com www.appliedbiosystems.com 58374r1_AB04499 12/15/04 10:50 PM Page 4 Instrument and Computer Dimensions Dimension 7500/7500 Fast System Notebook Tower Width 34 cm (13.39 in.) 32 cm (12.4 in.) 18 cm (7.1 in.) Depth 45 cm (17.72 in.) 26 cm (10.1 in.) 45 cm (17.6 in.) Height 49 cm (19.29 in.) 3 cm (1.2 in.
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