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ABI PRISM ® 3100 Genetic Analyzer

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ABI PRISM ® 3100 Genetic Analyzer ABI PRISM® 3100 Genetic Analyzer User’s Manual © Copyright 2001, Applied Biosystems For Research Use Only. Not for use in diagnostic procedures. NOTICE TO PURCHASER This instrument, Serial No. ________________, is Authorized for use in DNA sequencing and fragment analysis. This Authorization is included in the purchase price of this instrument and corresponds to the up-front fee component of a license under process claims of U.S. Patent Nos. 5,821,058 and 5,332,666 and under all process claims for DNA sequence and fragment analysis of U.S. patents now or hereafter owned or licensable by Applied Biosystems for which Authorization is required, and under corresponding process claims is foreign counterparts of the foregoing for which an Authorization is required. The running royalty component of licenses may be purchased from Applied Biosystems or obtained by using Authorized reagents purchased from Authorized supplier in accordance wit the label rights accompanying such reagents. Purchase of this instrument does not itself convey to the purchaser a complete license or right to perform the above processes. This instrument is also licensed under U.S. Patent No. 5,171,534 and apparatus and system claims in foreign counterparts thereof. No rights are granted expressly, by implication or by estoppel under composition claims or under other process or system claims owned or licensable by Applied Biosystems. For more information regarding licenses, please contact the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404. The ABI PRISM® 3100 Genetic Analyzer includes patented technology licensed from Hitachi, Ltd. as part of a strategic partnership between Applied Biosystems and Hitachi, Ltd., as well as patented technology of Applied Biosystems. ABI PRISM and its design, Applied Biosystems, BioLIMS, GeneScan, Genotyper, and MicroAmp are registered trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. ABI, BigDye, Factura, Hi-Di, POP, POP-4, and POP-6 are trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. AmpliTaq is a registered trademark of Roche Molecular Systems, Inc. Microsoft, Windows, and Windows NT are registered trademarks of the Microsoft Corporation in the United States and other countries. Oracle is a registered trademark of the Oracle Corporation. pGEM is a registered trademark of Promega Corporation. All other trademarks are the sole property of their respective owners. Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches into 150 countries on six continents. For international office locations, please call our local office or refer to our web site at www.appliedbiosystems.com. Record information about your software below. Software CD Serial Number Version Number Registration Code 3100 Software Oracle® for NT GeneScan® Application Sequencing Analysis Application Contents 1 Introduction and Safety Overview . 1-1 ABI PRISM 3100 Genetic Analyzer . 1-2 To Get Started Quickly. 1-3 Additional Documentation . 1-4 Safety . 1-5 2 System Overview Overview . 2-1 Section: 3100 Instrument Overview . 2-3 ABI PRISM 3100 System Components . 2-4 What the Instrument Does . 2-5 How the Instrument Works. 2-6 Section: Instrument Hardware Overview . 2-9 Front View . 2-10 Front View with Doors Open . 2-11 Back View. 2-12 Section: Computer and Software Overview . 2-13 Computer Workstation . 2-14 Software . 2-15 Section: Chemistry Overview . 2-17 Supported Dye Sets . 2-18 Labeling Chemistries . 2-19 Polymers. 2-20 Injection Solution . 2-21 Section: Electrophoresis Overview . 2-23 Capillary Array. 2-24 Electrophoresis . 2-25 Electrophoresis Circuit . 2-26 Section: Fluorescence Detection Overview . 2-27 Introduction . 2-28 Laser . 2-29 Transmission Grating . 2-29 CCD Camera . 2-29 i 3 Performing a Run Overview . 3-1 Section: Introduction . .3-3 Summary of Procedures. 3-4 Planning Your Runs . 3-5 Section: Working with Samples and Plate Assemblies . .3-7 Sample Preparation . 3-8 Working with Plate Assemblies. 3-9 Section: Starting the 3100 System . .3-11 Starting the Computer . 3-12 Starting the Instrument . 3-13 Starting the 3100 Data Collection Software . 3-14 Section: Checking the Available Space and Deleting Records . .3-15 Checking the Available Hard Drive Space . 3-16 Checking the Available Database Space . 3-17 Deleting Records from the Database . 3-17 Section: Preparing the Instrument . .3-19 Instrument Setup . 3-20 Preparing Buffer and Filling the Reservoirs . 3-22 Calibrating the Instrument. 3-24 Placing the Plate onto the Autosampler. 3-25 Section: Setting Up the Software . .3-27 Setting Software Preferences. 3-28 About Plate Records . 3-30 Creating a Plate Record for GeneScan Analysis . 3-31 Creating a Plate Record for DNA Sequencing Analysis . 3-36 Linking and Unlinking a Plate. 3-41 Section: Running the Instrument . .3-45 About Run Scheduling. ..
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