High-performance, flexible and reliable products that will set you up for success
thermofisher.com/biotechessentials Techniques like cell growth, modification and analysis, sample preparation, PCR, qPCR, sequencing, electrophoresis and western blotting are the building blocks of your projects. Getting these fundamentals right first-time is critical to you moving quickly and efficiently towards your end goal.
In order to do this, you need a partner who can supply all the right products and services. They need to be reliable, flexible, scalable and save you time so that you can minimise development and product costs, reduce risk, and reach your next milestone faster.
We have a whole range of solutions dedicated to serving these needs ranging from off-the-shelf and customisable reagents and instruments to outsourcing services and manufacturing solutions. Take a look through this brochure to find out more.
We’ll work with you every step of the way to ensure you’re set for success. Cell growth, scale-up and modification 4
Cellular analysis 6
Cellular analysis screening 8
Sample preparation 10
Synthetic biology 12
PCR 14
Electrophoresis 16
RNA extraction and cDNA synthesis for qPCR 17
Real-time PCR 18
Sanger and next-generation sequencing 20
Protein analysis 22
Outsourcing services 25
Custom manufacturing and commercial supply 26
Opening a new lab or relocating? 28
3 Cell Growth, Scale-up and Modification Whether you are culturing or modifying your cells, our range of Gibco™, Thermo Scientific™ Nunc™ and Invitrogen™ products will deliver the quality, consistency and performance you need at every step of your journey from discovery to commercialisation.
Cell growth and scale-up
Gibco™ Media and Supplements From the basic formulations to the newest innovations, Gibco™ products provide superior quality, consistency, and performance—to ensure you get it right first time.
• Gibco cell culture media is designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We’ve developed ready-to-use products as well as powdered and concentrated liquid formulations to fit your experimental set-up and budget. thermofisher.com/ media • Gibco™ BenchStable™ media is stored at room temperature so you don’t have to wait for your media to warm up—saving you valuable time. It also frees up your cold storage space and uses more sustainable packaging so you use less energy.
• Gibco supplements including balanced salt solutions, cell dissociation reagents, antibiotics and more are designed to deliver reproducibility.
Gibco™ Fetal Bovine Serum Used by 14 of the top 15 pharma companies and accounting for 45% of all FBS citations**, Gibco sera offers you the performance, quality and consistency you need to reduce the unknowns in your work and move quickly towards your goals.
We propose differentiated workflow solutions from specialty serum to innovative packaging like the aliquot-free Gibco™ One Shot FBS 50ml bottle.
thermofisher.com/ Whether you need fetal bovine serum with the least viral risk, the lowest fbs endotoxin levels, or sera qualified for specialty applications and assays, we have the right serum for you.
**2006–2015
4 Thermo Scientific™ Nunc™ and Nalgene™ Cell Culture Plastics Get the best results from your cell culture work with Nunc and Nalgene cell culture plastics. Manufactured using only high-quality raw materials that comply with USP Class VI testing and tested with Gibco media, our plastics will deliver you optimal cell growth across multiple cell lines.
Choose from a range of surfaces and formats all designed to make your cell thermofisher.com/ culture work easier and more scalable—flasks, dishes, plates, conical tubes cellcultureplastics and serological pipettes.
Cell modification
Invitrogen™ Neon™ Transfection System Obtain superior transfection efficiency regardless of your lab’s size, budget and needs with a system that will flex around your changing work:
• Efficient—up to 90% transfection and gene-editing efficiency in extremely difficult cells, including immune, primary and stem cells
• Flexible—open system allows electroporation parameters to be optimised freely; easily transfect from 2 x 104 cells to 6 x 106 cells per reaction thermofisher.com/ neon • Simple—single reagent kit for all cell types
• Versatile—can be used to deliver DNA, RNA and protein
Invitrogen™ Lipofectamine™ 3000 Transfection Reagent Get it right first time using our most advanced lipid nanotechnology:
• Superior performance—highest efficiency into the broadest spectrum of difficult to-transfect and common cell types
• Improved cell viability—gentle on your cells, with low toxicity thermofisher.com/ lipofectamine3000 • Versatility—single kit for DNA, RNA, and cotransfection
Invitrogen™ TrueCut™ Cas9 Protein v2 Move quickly with the next-generation Cas9 Protein:
• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem
• Up to 2X higher editing efficiency in difficult targets than the competition thermofisher.com/ crisprprotein • High quality—manufactured under strict ISO 13485 quality standards
5 Cellular Analysis Our cellular analysis product portfolio combines the strengths of Invitrogen™ fluorescent reagents and versatile analysis instrumentation. Discover more about your samples with automated cell counting, automated multiwell plate scanning, long-term live-cell imaging and powerful image analysis.
Cell counting
Invitrogen™ Countess™ II FL Automated Cell Counter Contains advanced autofocusing and counting algorithms to allow you to quickly and accurately count cells while avoiding user variation associated with manual counting.
Perform cell viability using Invitrogen™ LIVE/DEAD™ 74 labeling kits 80 70 Switch to counter with reusable slide 60 Switch to counter with 48 Obtain Apoptosis and viability data with a viability dye 50 disposable slide such as Invitrogen™ SYTOX™ Red Dead Cell Stain, 40 30 30 in combination with an apoptosis indicator that measures 15 19 20 caspase activation, such as Invitrogen™ CellEvent™ 10
Number of Hours Saved 10 Caspase-3/7 Green Detection Reagent. 0 5 Slides 10 Slides 25 Slides Average number of slides counted per day thermofisher.com/countess
Fluorescence imaging
Invitrogen™ EVOS™ M7000 Imaging System A powerful, fast, fully automated system for reliable results, first time. Bring high performance and fast, automated imaging right to your lab bench with the EVOS M7000 Imaging System. This system has been designed with advanced capabilities to simplify demanding cell-based imaging applications such as live-cell analysis, image tiling, and Z-stacking, so you can focus on acquiring images and data rather than instrument operation. When combined with the Invitrogen™ EVOS™ Onstage Incubator, the EVOS M7000 Imaging System is ideal for long-term monitoring of cell cultures and time-lapse imaging at high resolution. thermofisher.com/ evosm7000 Extensive quantitative imaging and statistical analysis can be performed in combination with Invitrogen™ Celleste™ Image Analysis Software, an optional advanced software package offering powerful tools for image segmentation and classification.
6 Cellular quantification / microplate detection
Thermo Scientific™ Varioskan LUX™ Multimode Microplate Reader Designed for the highest performance and highest quality of results in a wide variety of applications. Automatic dynamic range selection adjusts the optimal reading gain based on signal intensity; smart safety controls alert to potential errors before they happen, all to save your time and avoid experimental errors and get to your goals faster.
Varioskan LUX includes a range of measurement technologies: Absorbance, thermofisher.com/ Fluorescence Intensity, Luminescence, AlphaScreen, and TRF (Time-Resolved varioskanlux Fluorescence). Fast readout of signals across a range of measurement modes: end point, kinetic, multipoint, spectral scanning, and kinetic spectral scanning, enables a variety of cellular assays.
An integrated gas module enables precise control of CO2 and O2 levels for optimal cell growth.
Intuitive Thermo Scientific™ SkanIt™ Software provides powerful data analysis and calculations such as cell viability percentages and cytotoxic potency. For additional benefit and ease of use, SkanIt Software includes several ready-made protocols for cell health assays, such as for the Invitrogen™ alamarBlue™ and PrestoBlue™, multiplexing, ATP assays, DLR assays, and NanoBRET.
Varioskan LUX can be combined with the EVOS M7000 Imaging System for the fluorescence-based quantification and imaging of complex biological systems, without requiring separate assay optimisation.
Cell stains
Invtirogen™ ReadyProbes™ Imaging Reagents Ready-to-use imaging reagents are the easiest and quickest way to image your cells. Designed to allow you to stain cells with: no calculations, no dilutions, no pipetting. Simply apply and image.
• Dropper bottle formats • Stable at room temperature • Simplified protocols • No dilutions, resuspensions, calculations, or aliquoting necessary thermofisher.com/ readyprobes
7 Cell analysis screening
Invitrogen™ Attune™ NxT Flow Cytometer A benchtop analyser that uses a revolutionary technology—acoustic focusing, to align cells prior to interrogation with a laser for your multicolour flow cytometry analysis. Now with the flexibility to create a customised 4-laser, 14-colour system, the Attune NxT cytometer is designed to accommodate existing experimental protocols and lab budgets.
• Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min thermofisher.com/ attune • Intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
• Save time and minimise cell handling with faster protocols
• Expand your capabilities with up to 6 channels of detection off the violet laser
Save up to 90 minutes in preparation time
Generic sample preparation workflow
Single-cell Wash Lyse Wash Stain Wash Analyse suspension (5–10 min) (30–60 min) (5–10 min) (15–30 min) (5–10 min) (5–10 min)
65–130 min
No wash, no lyse sample preparation workflow
Single-cell Stain Analyse Up to suspension (15–30 min) Dilute 10x faster* (5–10 min) 65% reduction in 20–40 min prep time
*Compaired to conventional cycometers
8 High content screening
Thermo Scientific™ CellInsight™ CX7 LZR HCA Platform Bring automated quantitative cell analysis to your screening lab. The CellInsight™ CX7 offers a powerful and integrated way to develop and automate high-content assays, using a combination of 5-colour bright field and 7-colour fluorescence imaging. With laser illumination, the platform enables rapid imaging in both wide-field and confocal modes, with deep penetration into 3D samples.
• Faster scan times—up to 5x faster than confocal imaging thermofisher.com/ cx7 • Quantifiably brilliant data—laser-based illumination provides superior spheroid illumination
• Enhanced speed and sensitivity for superior performance—seven independent lasers provide superior multiplexing with minimal bleed through
A scalable system that will flex around your changing needs:
Scalable cell biology Connect directly to the Thermo Scientific™ Orbitor™ RS Microplate Mover to increase processing capacity up to 80 plates.
Screen thousands of samples HCS Studio Cell Analysis software accelerates your study with “On the fly” real-time analysis, enabling faster acquisition of data.
Scalable image storage Store Image and Database Management Software expands to a server-based platform, allowing huge amounts of images and data to be managed.
Services and Support for our Instruments Because science can’t wait
You have no time for downtime. Our superior services and support solutions will get you started quickly and help to keep your lab up and running. More than 1,000 trained professionals make-up the industry’s largest network, ready to assist you when you need it. And smart features such as remote instrument services help keep you productive and focused on making the next discovery.
thermofisher.com/instrumentservice
9 Sample Preparation Consistently extract and purify your nucleic acids, proteins and cells with maximum yield, purity and integrity from virtually any sample type. At Thermo Fisher Scientific, we are dedicated to innovation, so you can get it right first time.
Automated sample preparation
Thermo Scientific™ KingFisher Purification Systems KingFisher instruments provide the most versatile benchtop automation in the lab with consistent extraction and purification of DNA, RNA, Protein, and Cells.
• Vary your throughput—process 6–96 samples per run depending on the instrument model
• Interchange between formats—choose 24- or 96-well plates so you can process a wide range of input volumes
• Customise your protocol—easily edit, modify, or create new protocols
• Use optimised reagents—compatible with multiple magnetic-bead reagents
They help you to save valuable time by removing manual steps and reducing overall processing time, even when compared with other instruments.
Simplify your protocol
Select Prep plates Load plates Start run Program
1 2 3 4
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10 Consistent results, in less time
75% less touch time
gDNA Average time required from blood using our automated solutions 50% less touch time Average time for manual sample prep cfDNA
75% Not ready for automation? Check less touch time out our manual nucleotide sample prep options at: thermofisher.com/ RNA rocktheprep
thermofisher.com/kingfisher
Kits and reagents for KingFisher instruments
Applied Biosystems™ MagMAX™ DNA/RNA Purification Kits
• Versatile—suitable for low to high throughput applications
• Greater sample range—efficient removal of inhibitors from a wide range of samples with different viscosities
Invitrogen™ Dynabeads™ and Thermo Scientific Pierce magnetic agarose beads
• Use the gold standard for optimum results—Dynabeads offer the best balance of high yield and reproducibility with low nonspecific binding for Immunoprecipitation.
• More choice to suit your research—multiple ligands available for different protein purification strategies, including immobilised protein A/G, Ni-NTA, glutathione, and anti-FLAG.
11 Synthetic Biology From DNA and RNA oligonucleotides to complete gene synthesis and services, we offer it all. With a variety of custom and pre-designed products for a multitude of applications, our innovations are designed to get you to your next milestone quickly, whatever your requirements.
Invitrogen™ Oligonucleotides
1. Custom DNA and RNAi oligos 2. Pre-designed DNA oligos and primers Our Invitrogen™ custom oligo service has been In addition to our custom synthesis, we offer a selection of supporting researchers all over the world for over 20 other high-quality, pre-designed DNA primers and oligos years. We offer a complete range of custom synthesised that are commonly used in a variety of molecular biology oligonucleotide primers, probes, and genes. Our oligos and genomic applications, such as: can be used in a variety of applications including: • Expression analyses • Research: PCR, qPCR, cloning, sequencing, and gene synthesis & detection • RNAi-based studies
• Diagnostics: diagnostic tests • Epigenetic research
• Therapeutics: DNA/Antisense, RNAi • Next-generation sequencing and Immunotherapy
No time to place an order? Check out our fast, cost-effective way to order your oligos: thermofisher.com/valueoligos
12 GeneArt High-Q Strings 90 & Strings DNA Fragments 80 77 & Libraries 70 60 • Custom-made, uncloned, double- 50 stranded linear DNA fragments 40
30 • Strings Fragments can be used to 20 16 quickly clone your gene of interest 11 10 5 7 5 5 2 3 2 3 3 0 1,000 1,500 3,000 5,000 Insert length (bp)
thermofisher.com/ GeneArt High-Q Strings DNA Fragments Supplier 1 or 2 strings (p=0.98) find one correct clones to no. of screened GeneArt Strings DNA Fragments
GeneArt Genes and Sub-Cloning Services
• Increase your efficiency—expression-ready genes, subcloning, variants, libraries and more
• Maximise your protein yield—optional sequence processing with GeneOptimizer software generates sequence variants with enhanced mRNA stability and translational efficiency
• “GeneArt it“—save time and labour by outsourcing your gene synthesis and cloning so you can focus on your research
Find out more about our GeneArt products and services at: thermofisher.com/geneart
13 PCR Consistently arrive at results quicker, with more assurance and less optimisation, using our scalable ProFlex™ PCR System, innovative Platinum Hot-Start Polymerases and reliable MicroAmp™ PCR Plastics.
Thermal cyclers
Applied Biosystems™ ProFlex™ PCR System Ultimate flexibility that scales up or down supporting the evolution of your assay development.
• Interchangeable block formats—3x23 well, 96 well, dual 96 well, dual 384 well and dual flat 96 well thermofisher.com/ proflex • Run up to 6 experiments at once and up to 3 different users
• Precise control of the PCR temperature thanks to our patented VeriFlex™ Block temperature control technology
• Cloud enabled system
More than 1 thermal cycler? Ultimate Control with Fleet Control Software View and control your thermal cycler inventory in your facility securely behind your firewall with a central “server” computer
• Create customer permissions and rules for users, methods and instruments
thermofisher.com/ • Ensure data integrity with audit trail fleetcontrol
thermofisher.com/pcr
14 PCR plastics and DNA polymerases
Applied Biosystems™ MicroAmp™ PCR plastics
• Validated on Applied Biosystems thermal cyclers for optimal fit and performance
• Available in all formats to support your needs
Invitrogen™ Platinum™ SuperFi™ II DNA Polymerase thermofisher.com/ findplastics
• Exceptional accuracy—higher than 300x Taq fidelity as determined by next-generation sequencing
• Simplified workflow—buffer formulated for primer annealing at 60°C (no need for a Tm calculator)
• Increased PCR success—robust amplification of GC-rich targets, DNA of suboptimal purity, and long sequences
• Improved automation—high specificity and benchtop stability for 24 hours after reaction setup, enabled by Invitrogen™ Platinum™ thermofisher.com/ Hot-Start technology superfi
• Reduced pipetting—master mix options available with or without direct gel-loading dyes
Platinum SuperFi II enzyme Get it right first time NEB Q5 Hot Start High- Fidelity enzyme Takara primeSTAR GXL enzyme with an extremely low Merck KOD Hot Start enzyme KAPA HiFi Hot Start enzyme error rate Qiagen HotStar HiFidelity enzyme Taq enzyme
0 100 200 300
Fidelity compared to Taq enzyme
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
• Innovative universal primer annealing buffer—reduces tedious optimisation and saves time by enabling co-cycling of all assays together
• Engineered Taq DNA polymerase—allows fast cycling and successful amplification even in the presence of inhibitors
• Platinum hot-start technology—enables superior specificity, sensitivity, thermofisher.com/ and yields; allows for room temperature reaction setup platinumiitaq
15 Electrophoresis Using precast agarose gels can simplify and speed up your nucleic acid electrophoresis workflow. There are no gels to pour, buffers to make, staining or destaining steps to perform, or gel boxes to assemble. Just load your samples and start the run.
Invitrogen™ E-Gel™ Power Snap Electrophoresis System Combine rapid, real-time nucleic acid analysis with high-resolution image capture for unmatched convenience.
• Analyse faster—sample loading to image capture in 15 minutes
• Simple operation—intuitive user interface with large touch screen and integrated operating system
thermofisher.com/ • Safer handling—minimise handling of hazardous chemicals with powersnap Invitrogen™ E-Gel™ precast gel cassettes
Invitrogen™ E-Gel™ Precast Gels
• Minimise time-consuming and messy prep work
• Run up to twice as fast as conventional handcast gels
• Available in a variety of agarose percentages, well formats, and throughput capacities to suit your applications
thermofisher.com/ egel Need a higher throughput? Accelerate high-throughput electrophoresis analysis with E-Gel 48 and E-Gel 96 precast gels run on our expandable Invitrogen™ E-Base™ Electrophoresis System. The integrated design of the Invitrogen™ Mother E-Base™ and Daughter E-Base™ devices saves space and allows for up to 384 samples to run at one time.
16 RNA Extraction and cDNA Synthesis for qPCR Kits and reagents that save time and deliver superior performance with even the most challenging samples.
Invitrogen™ Cells-to-CT Kits
Our Cells-to-CT kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells, so there is no need to purify RNA.
• Extraordinary ease and speed—96 samples for qRT-PCR in typically <10 min
• No tedious RNA purification—no columns, heating, or centrifugation
• Superior performance—designed for consistent accuracy, reproducibility, thermofisher.com/ and sensitivity with 1–100,000 cells cellstoct
• Validated kit workflows—complete sets of reagents pre-optimised to work efficiently right out of the box
Invitrogen™ SuperScript™ IV Invitrogen™ Superscript™ IV VILO™ Reverse Transcriptase Master Mix Engineered to deliver superior performance even with challenging RNA samples. • Formulated to deliver exceptional performance in RT-qPCR applications • Super-efficient—up to 100x higher cDNA yield
• Improved Ct—up to 8 fewer cycles compared to other • Super robust—transcribes even from low abundant, RT reagents degraded or inhibitor-containing RNA samples • Superior linearity—works with a broad range of • Super-fast—10 min cDNA synthesis protocol input RNA
• Super convenient—available in a variety of formats • Super-reliable—Improved RT-qPCR data reproducibility due to single-tube master mix format • Extensive quality testing • Super-safe—Integrated, easy, and RNA-friendly genomic DNA (gDNA) removal
• Extensive quality testing
thermofisher.com/ thermofisher.com/ ssiv ssiv
17 Real-Time PCR The Applied BiosystemsTM Real-Time PCR (qPCR) offering of integrated and scalable solutions will ensure you get it right first time. High-performing consumables and innovative qPCR instruments give you anywhere, anytime access to your data and world class service and support.
Our predesigned Applied BiosystemsTM TaqMan® Assays which are available in 5 different formats for 29 species, will help you to spend your time generating results, not designing and optimising assays.
qPCR systems
Applied Biosystems™ QuantStudio™ 6 and 7 Pro Real-Time PCR Systems The QuantStudio 6 and 7 Pro systems are designed to make your qPCR more personalised, efficient, simple and productive. The smart workflow experience brings lab-changing qPCR innovations to your research:
• Interchangeable blocks
thermofisher.com/ • Automation quantstudiopro
• Manual error reduction—compatible with TaqMan Array Plates with RFID
• Smart features—hands-free functionalities like voice commands and facial authentication
• Superior service and support with Smart Help and Smart Remote Support qPCR assays
Applied Biosystems™ TaqMan® Assays
TaqMan Assay technology is the gold standard in performance, quality, and content for analysis by real-time PCR. We offer a unique end-to-end solution so you can spend your time generating results, not designing and optimising assays.
Choose from over 2 million pre-designed gene expression assays or more than 18 million SNP and CNV genotyping assays, all covered by the TaqMan thermofisher.com/ Assays Guarantee. We have a complete suite of solutions that will enable taqman you to get fast, reliable, and accurate results everytime.
18 qPCR reagents
Our optimised master mixes deliver accuracy and specificity first time and every time.
Recommended high performing qPCR reagents for 2-step Gene Expression:
Applied Biosystems™ PowerUp™ SYBR Green Master Mix Exceptional specificity with dual hot-start mechanism for gene expression and miRNA detection experiments. Engineered for enhanced benchtop stability— stable at room temperature for up to 72 hours in preassembled reactions. thermofisher.com/ sybr Applied Biosystems™ TaqMan® Fast Advanced Master Mix Superior accuracy, dynamic range and specificity compared to standard master mixes for gene expression and miRNA detection experiments. Engineered for thermofisher.com/ enhanced benchtop stability—stable at room temperature for up to 72 hours in qpcrmm preassembled reactions. Use with Applied Biosystems™ TaqMan® Assays.
Recommended high performing qPCR reagent for Genotyping:
Applied Biosystems™ TaqPath™ proAmp Master Mix Accurate results from both pure and crude samples containing PCR inhibitors for your SNP genotyping and copy number variation experiments.
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labelled “For Laboratory Use.” Use with Applied Biosystems™ TaqMan® Assays. thermofisher.com/ taqpath Recommended high performing qPCR reagent for 1-step Real-Time PCR:
Applied Biosystems™ TaqMan® Fast Virus 1-step Master Mix Accurate results from pure and challenging samples (e.g. blood, stool) that may contain PCR inhibitors.
The 4x formulation provides enhanced detection of both RNA and DNA viruses, even with low target input. Use with Applied Biosystems™ TaqMan® Assays. thermofisher.com/ qpcrmm
19 Sanger and Next-Generation Sequencing Solutions It has never been easier to get fast access to great data from Sanger and next-generation sequencing.
Sanger sequencing
Ion Torrent™ Ion GeneStudio™ NGS Series Obtain gold-standard sequencing and fragment analysis without the hassle of capillary set-up or technical know-how. Add your sequencing samples to the cartridge, click it in place and get your results without having to wait in the laboratory.
Ideal for checking your clones or cell line authenticity, human identification applications, or mutation detection and NGS results verification, this handy benchtop instrument is a low-cost, low throughput solution to everyday thermofisher.com/ sequencing needs. seqstudio
• Universal all-in-one cartridge—this novel system design allows for an on- instrument reagent life of four to six months for maximum convenience
• Reduced setup time—combine sequencing and fragment analysis runs on the same plate
• Speed—receive data for up to 4 samples within 4 hours
• Easy to access, analyse, and share data anytime, anywhere, with Connect, our cloud-based platform
Next-generation sequencing
Invitrogen™ Collibri™ NGS Library Preparation Kits for Illumina Systems The Collibri portfolio of library prep kits and accessories generate libraries more quickly, with less bias, in order to match the speed and capacity of Illumina instruments. Advantages available only from Collibri Library Prep Kits:
• Visual feedback for higher library prep success
• Shorter total workflow times, greater support thermofisher.com/ collibri • Reduced bias in genomic interpretation from whole genome sequencing
• Clean data reveals transcriptome complexity from whole transcriptome sequencing
20 Ion Torrent™ Ion GeneStudio™ S5 System Designed to optimise throughput and cost, for both small and large targeted NGS projects including, gene panel sequencing, whole transcriptome, gene pathway expression profiling, biomarker discovery in cancer research or identifying microbial species in mixed samples. You can automate your sequencing library, sequencing preparation and chip loading with the Ion Chef™ System and access data analysis solutions using Ion Reporter™ Software on the cloud or on your own server.
• Five Ion S5™ chip options enable a wide range of applications
• Sequencing and analysis in as little as 3 hours
• Built to work seamlessly with Ion AmpliSeq™ and Ion AmpliSeq™ HD gene panels; from just 1 ng of input DNA or RNA
• Integrated data interpretation software solutions or easy solutions for data export into your own thermofisher.com/ genestudio Identify new biomarkers with Ion™ AmpliSeq™ panels for targeted NGS Sequencing success with a simple workflow and low input of DNA or RNA.
• Small sample input—lowest DNA or RNA input requirement for targeted NGS
• Scalable—one to hundreds of gene targets in a single run
• Fast automated workflow—from sample to data in as little as 24 hours, with just 45 minutes of hands-on time thermofisher.com/ ampliseq Ion Torrent™ Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit Enable fast and affordable gene expression analysis with simultaneous amplification of more than 20,000 RefSeq genes in a single tube.
• Starting from as little as 10 ng of total RNA
• Fast gene expression profiling with simple workflow
• Streamlined data analysis with Torrent Suite Software thermofisher.com/ ampliseqtranscriptome Investigating T-cells? Gain deeper insights by harnessing the power of the immune repertoire with NGS.
The Ion Torrent™ portfolio of immuno-oncology NGS assays help you to understand the tumour microenvironment and investigate the immune thermofisher.com/ repertoire by interrogating all regions of the TCR beta chain—ideal for immuno-oncology-solutions identifying biomarkers correlating with tumour response.
21 Protein Analysis We offer a comprehensive suite of solutions for every step of your western blotting workflow, so you can get to your next milestone faster with maximum results and minimum hands-on-time.
Separate
Our versatile systems allow you to perform multiple types of gel runs and experiments quickly and easily.
Invitrogen™ Mini Gel Tank Convenient electrophoresis with a versatile tank compatible with more than 180 gels. It has an innovative side-by-side design for clear visualisation and faster sample loading and also requires less running buffer.
Invitrogen™ PowerEase™ 300W Power Supply (230 VAC) The PowerEase 300W Power Supply is a fully programmable power supply designed for high-throughput gel electrophoresis. The straight-forward, intuitive interface makes the powering of gel runs a simple and easy process.
Thermo Scientific™ and Invitrogen™ Protein Ladders Protein ladders that are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity.
Invitrogen™ Bolt™ Bis-Tris Gels Bolt Bis-Tris Plus Gels are designed to deliver consistent gel performance with a neutral pH environment that minimises protein modifications. Bolt gels are the best choice for any protein experiment where protein integrity is crucial.
Invitrogen™ Bolt™ Buffers Bolt buffers and sample reducing agents are optimised to prepare your protein sample to be ran with Bolt Bis-Tris Plus gels.
thermofisher.com/ separate
22 Transfer Move rapidly through the protein transfer step with our dry and semi-dry blotting systems.
Invitrogen™ Power Blotter XL System Specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 12 minutes. Includes an integrated power supply optimised to enable consistent and high-efficiency protein transfer.
Invitrogen™ iBlot™ 2 Gel Transfer Device Performs western blotting transfer simply, efficiently, and reliably, thermofisher.com/ within seven minutes and without the need for liquid buffers. transfer
Detect Obtain quick visualisation, precise quantitation, single-probe detection or multiplexing every time.
Invitrogen™ iBind™ Flex Western Device An automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow. Set-up time is fast: within 2.5 hours you are ready for the detection step.
Invitrogen™ Secondary Antibodies
• Anti-Rabbit secondary antibody affinity-purified with well-characterised specificity for rabbit immunoglobulins conjugated with HRP for western- blot detection.
• Anti-Mouse secondary antibody affinity-purified with well-characterised specificity for mouse immunoglobulins conjugated with Alexa Fluor Plus 488 for western-blot detection.
Thermo Scientific™ SuperSignal™ Chemiluminescent Substrates
• SuperSignal West Pico PLUS Chemiluminescent Substrate—an enhanced chemiluminescent horseradish peroxidase substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
• SuperSignal West Femto Maximum Sensitivity Substrate—an ultra-sensitive enhanced chemiluminescent substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase enzyme.
Invitrogen™ No-Stain™ Protein Labeling Reagent The Invitrogen™ No-Stain™ Protein Labeling Reagent is a fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that thermofisher.com/ provides sensitive, linear detection of protein for total protein normalization detect (TPN) of western blotting data.
23 Thermo Scientific™ Restore™ Western Blot Stripping Buffers Specially formulated to dissociate and strip antibodies from western blots so that membranes can be reprobed under alternate conditions or with another antibody to detect a different protein target. iBright™ Imaging Systems Capture images and analyse data from chemiluminescent and fluorescent western blots efficiently and easily. These high-performance instruments thermofisher.com/ enhance the imaging experience through powerful hardware, advanced detect automated technologies, and an interface that is easy to use for researchers of all experience levels.
Quantify
Invitrogen™ ProQuantum™ High-Sensitivity Immunoassays thermofisher.com/ Move fast with these ready-to-use kits that provide an easy method of proquantum measuring protein analytes. They use a very small amount of sample and have an extremely simple workflow that allows you to move from sample to results in just 2 hours.
Thermo Scientific™ Multiskan™ Sky Microplate Spectrophotometer Refined photometry for life sciences research and unmatched versatility for protein analysis.
• Ready-to-use built-in protocols for protein quantification
• Read your sample in a microplate (even a half-area microplate), cuvette, or µDrop plate for micro-volume protein sample measurements thermofisher.com/ multiskan • Save your results to a USB memory device, local area network, or Thermo Fisher Cloud
• Thermo Scientific™ SkanIt™ Software provides ease of use and convenience with the inclusion of several ready-made protocols for protein work.
Invitrogen™ Qubit™ 4 Fluorometer Quickly and specifically quantitate DNA or RNA or non-degraded proteins.
• Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more sensitive than UV absorbance
• Uses as little as 1 μL of sample, even with very dilute samples
thermofisher.com/ • New integrated reagent calculator to quickly generate qubit working solution calculations
24 Strategic Outsourcing: Custom Research Services Whether your work requires specialised, reliable, high-quality solutions that you don’t have the capability to handle in-house, or you are facing resource constraints for more routine tasks, we have an outsourcing solution for you.
Our specialists will work with you to build the optimal workflow with a secure chain of custody.
Simplifying your work Fast-track your timelines across a broad portfolio Our dedicated and focused project management team of services will help accelerate your time
to discovery via a global • Cell Line Generation network of R&D support. Leverage our experience in lab • Gene to Protein essentials as a • Gene Editing using CRISPR or TALs powerful resource Decrease FTE hands-on time to augment your Free up your skilled R&D research team scientists (and lab space) from • Gene Delivery Services routine everyday tasks allowing — from routine them to focus on strategically experiments, to important initiatives. • Custom Biology Services the most recent and innovative • Custom Peptides, Antibody and Access new Conjugation Services techniques. technologies and expertise Benefit from connections to unique skill sets and • Screening and Lead ID Services specialised resources. Our R&D breakthroughs are translated into service thermofisher.com/ offerings that will help customservices you achieve your goals.
Contact us today to find out more: [email protected]
25 Custom Manufacturing and Commercial Supply As the world’s leading supplier of reagents and instruments for molecular biology, cellular imaging, synthetic biology, and protein sciences, we also offer the most comprehensive technology and product portfolio for use by companies in developing next-generation molecular testing and immunodetection assays. Our core capabilities include partnering with you for custom solutions, packaging and bulk supply but also can help minimise development and product costs, reduce risk, and bring your product to market faster.
Why choose Thermo Fisher Scientific?
Creating a high-performing, competitively positioned assay is demanding and challenging. We understand this challenge from our work with leading biotech and diagnostics companies who face many of the same difficulties you do. By choosing us, you gain an experienced ally with a dedicated diagnostics partnering business that is always available for your projects. Our customised solutions enable you to get the best possible assay, and our experienced team can help troubleshoot when you hit snags in development or post-launch.
We are the single supplier to help you accelerate assay development and kit manufacturing.
Manufacture products Licensing and supply agreements Customise • cGMP/QSR product design as needed • ISO 9001/13485 Select • Private labeling ≥5,000 patents products from • Custom packaging our portfolio and licenses • Global distribution Evaluate • Product workflow knowledge needs • Process control Extensive collection of technologies and tools for use in Experienced immunodiagnostic representatives development with technology and market experience
26 Commercial supply capabilities
Customisation From assay design to workflow optimisation—whatever you needs, our custom services team is there for you, including:
• Custom formulation with in-process testing • Custom packaging and labelling • Lyophilization • Custom functional testing (e.g., bridging studies, verification testing, stability studies, etc.) • Packaging options from single-use packages to large-volume carboys • Supply of products in any scale and in any format • Private labeling and kit production
Superior quality Don’t cut corners when it comes to quality. Bring your vision into being in our state-of-the-art manufacturing centers. Your product will be subjected to rigorous testing to ensure lot-to-lot consistency and reproducibility with the following compliance:
• Certified quality systems with ISO 9001, ISO 13485, • Good laboratory practice (GLP) –accredited and ISO 14001 status • Manufacturing and filling within facilities that meet • Six Sigma and Lean Manufacturing processes 100,000 (class D) clean-room standards
Robust security of Supply Partnering with us will ensure the security of your raw material supply. We have strong safeguards in place to help you reduce risk and avoid delays and setbacks in manufacturing, including:
• Advanced change control notifications • Multiple manufacturing sites • Supply agreements • Financial stability • A global manufacturing footprint • Confidentiality • Comprehensive controls over raw materials and processes
thermofisher.com/ oem
27 Get your lab up and running with up to 40%* OFF
The Jump Start New Lab Programme gives you Who can apply? exclusive discounts on some of the key products you need to set up your lab. You are eligible for the programme if you are:
All you need to do is apply online and one of our • Opening a new lab within your current institution account managers will be in touch to create a or company special quote for you. You will also receive a Jump Start Welcome Pack. • Relocating to a new space
• Starting a new company
Visit thermofisher.com/jumpstart to find out more and apply
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