DOCSLIB.ORG

Applied Biosystems 7500/7500 Fast Real-Time PCR System Presence/Absence Experiments

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Applied Biosystems 7500/7500 Fast Real-Time PCR System Presence/Absence Experiments Getting Started Guide Applied Biosystems 7500/7500 Fast Real-Time PCR System Presence/Absence Experiments Getting Started Guide Get Started Applied Biosystems 7500/7500 Fast Real-Time PCR System Presence/Absence Experiments Design the Experiment Prepare the Reactions Run the Experiment Analyze the Experiment © Copyright 2007, 2010 Applied Biosystems. All rights reserved. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. For Research Use Only. Not for use in diagnostic procedures. NOTICE TO PURCHASER: Label License The 7500/7500 Fast Real-Time PCR System is covered by US patents and corresponding claims in their non-US counterparts, owned by Applied Biosys- tems. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5 nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. TRADEMARKS: Applera, Applied Biosystems, AB (Design), MicroAmp, NucPrep, PrepMan, Primer Express, and VIC are registered trademarks and FAM, JOE, NED, and ROX are trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries. AmpErase, AmpliTaq Gold, and TaqMan are registered trademarks of Roche Molecular Systems, Inc. SYBR is a registered trademark of Molecular Probes, Inc. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other trademarks are the sole property of their respective owners. Part Number 4387785 Rev. C 06/2010 Contents Preface . vii How to Use This Guide . vii How to Obtain More Information . ix How to Obtain Support . xi Safety Conventions Used in This Document . xii Symbols on Instruments . xiii Safety Labels on Instruments . xiv General Instrument Safety . xvi Chemical Safety . xvii Chemical Waste Safety . xix Electrical Safety . xx Physical Hazard Safety . xx Biological Hazard Safety . xxi Workstation Safety . xxi Safety and Electromagnetic Compatibility (EMC) Standards . xxii Chapter 1 Get Started. 1 About the 7500/7500 Fast System . 2 Supported Consumables . 4 About Presence/Absence Experiments . 7 How to Use This Guide . 11 About the Example Presence/Absence Experiment . 12 Example Experiment Workflow . 14 Chapter 2 Design the Experiment . 15 Chapter Overview . 16 Create a New Experiment . 17 Define the Experiment Properties . 18 Define the Methods and Materials . 20 Set Up the Targets . 22 Set Up the Samples and Replicates . 23 Set Up the Run Method . 25 Review the Reaction Setup . 27 Order Materials for the Experiment . 31 Finish the Design Wizard . 33 Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Presence/Absence iii Experiments Chapter 3 Prepare the Reactions . 37 Chapter Overview . 38 Prepare the Template . 39 Prepare the Samples . 40 Prepare the Reaction Mix . 42 Prepare the Reactions . 44 Chapter 4 Run the Experiment . 49 Chapter Overview . 50 Prepare for the Run . 51 Enable the Notification Settings (Optional) . 53 Start the Run . 55 Monitor the Run . 55 Unload the Instrument . 57 Chapter 5 Analyze the Experiment . 59 Chapter Overview . 60 Section 5.1: Review Results . 61 Analyze the Experiment . 62 View the Presence/Absence Plot . 65 View the Amplification Plot . 67 View the Well Table . 74 Publish the Data . 77 Section 5.2: Troubleshoot (If Needed) . 79 View the Analysis Settings . 80 View the QC Summary . 82 Omit Wells from the Analysis . 84 View the Multicomponent Plot . 86 View the Raw Data Plot . 88 Appendix A Alternate Experiment Workflows . 91 Advanced Setup Workflow . 92 QuickStart Workflow . 93 Template Workflow . 95 Export/Import Workflow . 96 Bibliography . 99 iv Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Presence/Absence Experiments Glossary . 101 Index . ..
Load more

Recommended publications
  • Applied Biosystems 3730 and 3730Xl DNA Analyzers
    SPECIFICATION SHEET 3730 and 3730xl DNA Analyzers Applied Biosystems 3730 and 3730xl DNA Analyzers Introduction Applied Biosystems 3730 & 3730xl DNA Analyzers were developed to meet the growing needs of institutions ranging from core and research labs in academia, government, and medicine to biotechnology, pharmaceuticals, and genome centers. These high-throughput instruments couple advances in automation and optics with proprietary Applied Biosystems reagents and software to support a diverse range Key Features Key Benefits of genetic analysis projects. By • Dual-side capillary illumination • Highest-quality DNA sequencing data dramatically improving data quality, at lowest cost • Backside-thinned CCD significantly reducing total cost per – POP-7™ Polymer separation matrix sample, and enabling more runs per • Integrated auto-sampler and sample increases read length and reduces day, 3730/3730xl DNA Analyzers make plate stacker run time it quicker and easier for investigators • Onboard piercing station to get meaningful results in evolving – Multiple run modules provide options genomic applications. Whether your • Internal barcode reader for targeted length of read lab is involved in de novo sequencing, • Onboard polymer for up to 100 runs – High optical sensitivity reduces DNA resequencing, microsatellite-based and reagent consumption fragment analysis, or SNP genotyping, • Automated basecalling and quality 3730/3730xl DNA Analyzers are the value assignment – In-capillary detection consumes ideal platform for better, faster, cheaper •
    [Show full text]
  • Automated DNA Sequencing
    Automated DNA Sequencing Chemistry Guide ©Copyright 1998, The Perkin-Elmer Corporation This product is for research purposes only. ABI PRISM, MicroAmp, and Perkin-Elmer are registered trademarks of The Perkin-Elmer Corporation. ABI, ABI PRISM, Applied Biosystems, BigDye, CATALYST, PE, PE Applied Biosystems, POP, POP-4, POP-6, and Primer Express are trademarks of The Perkin-Elmer Corporation. AmpliTaq, AmpliTaq Gold, and GeneAmp are registered trademarks of Roche Molecular Systems, Inc. Centricon is a registered trademark of W. R. Grace and Co. Centri-Sep is a trademark of Princeton Separations, Inc. Long Ranger is a trademark of The FMC Corporation. Macintosh and Power Macintosh are registered trademarks of Apple Computer, Inc. pGEM is a registered trademark of Promega Corporation. Contents 1 Introduction. 1-1 New DNA Sequencing Chemistry Guide . 1-1 Introduction to Automated DNA Sequencing . 1-2 ABI PRISM Sequencing Chemistries . 1-5 PE Applied Biosystems DNA Sequencing Instruments . 1-7 Data Collection and Analysis Settings . 1-12 2 ABI PRISM DNA Sequencing Chemistries . 2-1 Overview . 2-1 Dye Terminator Cycle Sequencing Kits . 2-2 Dye Primer Cycle Sequencing Kits . 2-8 Dye Spectra . 2-12 Chemistry/Instrument/Filter Set Compatibilities . 2-13 Dye/Base Relationships for Sequencing Chemistries . 2-14 Choosing a Sequencing Chemistry. 2-15 3 Performing DNA Sequencing Reactions . 3-1 Overview . 3-1 DNA Template Preparation . 3-2 Sequencing PCR Templates . 3-10 DNA Template Quality. 3-15 DNA Template Quantity. 3-17 Primer Design and Quantitation . 3-18 Reagent and Equipment Considerations. 3-20 Preparing Cycle Sequencing Reactions . 3-21 Cycle Sequencing . 3-27 Preparing Extension Products for Electrophoresis .
    [Show full text]
  • Document Title: Development and Evaluation of a Whole Genome Amplification Method for Accurate Multiplex STR Genotyping of Compromised Forensic Casework Samples
    The author(s) shown below used Federal funds provided by the U.S. Department of Justice and prepared the following final report: Document Title: Development and Evaluation of a Whole Genome Amplification Method for Accurate Multiplex STR Genotyping of Compromised Forensic Casework Samples Author: Tracey Dawson Cruz, Ph.D. Document No.: 227501 Date Received: July 2009 Award Number: 2005-DA-BX-K002 This report has not been published by the U.S. Department of Justice. To provide better customer service, NCJRS has made this Federally- funded grant final report available electronically in addition to traditional paper copies. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S. Department of Justice. This document is a research report submitted to the U.S. Department of Justice. This report has not been published by the Department. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S. Department of Justice. FINAL TECHNICAL REPORT Development and Evaluation of a Whole Genome Amplification Method for Accurate Multiplex STR Genotyping of Compromised Forensic Casework Samples NIJ Award #: 2005-DA-BX-K002 Author: Tracey Dawson Cruz 1 This document is a research report submitted to the U.S. Department of Justice. This report has not been published by the Department. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S.
    [Show full text]
  • Applied Biosystems 7500 Real-Time PCR System and Applied Biosystems 7500 Fast Real-Time PCR System a Real Fast and Real Versatile Approach to Real-Time PCR
    58374r1_AB04499 12/15/04 10:50 PM Page 1 Specification Sheet Real-Time PCR Systems Applied Biosystems 7500 Real-Time PCR System and Applied Biosystems 7500 Fast Real-Time PCR System A Real Fast and Real Versatile Approach to Real-Time PCR • The 7500 Fast Real-Time PCR System enables standard 96-well format high speed thermal cycling, significantly reducing your run time for quantitative real-time PCR applications, deliv- ering results in about 35 minutes Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR System • The 7500 Real-Time PCR System is a versatile, leading-edge platform providing enhanced performance •Small instrument footprint permits Fast Thermal Cycling capabilities and an upgrade path easy placement in any laboratory, The 7500 System is available in two to high-speed thermal cycling even those with limited space configurations; the 7500 Fast System configuration with a high-speed, 96- •Five-color detection system provides Introduction well format thermal cycling block the flexibility to perform a variety The Applied Biosystems 7500 and capable of quantitative real-time PCR of applications including gene 7500 Fast Real-Time PCR Systems are runs in about 35 minutes, and the expression analysis, SNP genotyp- integrated, versatile platforms for the standard configuration with a 96-well ing and plus/minus assays that detection and quantification of nucleic format thermal cycling block with utilize internal positive controls acid sequences. Real-time PCR com- run times of under 2 hours. A service bines thermal cycling, fluorescence •Advanced optical configuration engineer installed Fast Upgrade Kit detection, and application-specific supports a broader range of fluo- is available for the 7500 System to software to measure the cycle-by-cycle rophores, including FAM™/SYBR® upgrade it to the Fast configuration.
    [Show full text]
  • APPLERA CORPORATION Annual Report
    312849_Applera_REVISED0914.qxd 9/17/01 6:42 PM Page 2 2001APPLERA CORPORATION Annual Report APPLIED BIOSYSTEMS CELERA GENOMICS 312849_Applera_REVISED0914.qxd 9/17/01 6:42 PM Page 3 Table of CONTENTS Letter to Stockholders Applera Corporation 3 Applied Biosystems Group 5 Celera Genomics Group 7 Celera Diagnostics 8 Financial Review 10 Directors and Officers 109 Stockholder Information 110 APPLERA CORPORATION Mission: To provide the world’s leading technology and information solutions that help life scientists understand and use the power of biology. Business Groups: Applied Biosystems and Celera Genomics Headquarters: Norwalk, Connecticut APPLIED BIOSYSTEMS GROUP Profile: A leading provider of technology solutions for life science research and related applications, with customers in over 100 countries. Headquarters: Foster City, California New York Stock Exchange Symbol: ABI CELERA GENOMICS GROUP Profile: A biopharmaceutical business and leading provider of genomic and related medical information, enabling therapeutics discovery using proprietary scientific capabilities for the group’s internal efforts and in partnership with pharmaceutical and biotechnology companies. Headquarters: Rockville, Maryland New York Stock Exchange Symbol: CRA CELERA DIAGNOSTICS Profile: A joint venture between Applied Biosystems and Celera Genomics that is leveraging capabilities from both businesses to develop new molecular and protein diagnostics. Headquarters: Alameda, California 312849_Applera_REVISED0914.qxd 9/17/01 6:42 PM Page 4 To our stockholders: Applera Corporation continues to be a leader This should allow us to more effectively in the life science revolution – a revolution many anticipate customers’ needs and bring products believe could define this new century. We enter to market in a more timely manner, while fiscal year 2002 at the threshold of major maintaining the technological innovation that advances in the field of disease diagnosis and has made us a premier provider of life science therapeutics discoveries, made possible in part by tools.
    [Show full text]
  • List of SARS-Cov-2 Diagnostic Test Kits and Equipments Eligible For
    Version 33 2021-09-24 List of SARS-CoV-2 Diagnostic test kits and equipments eligible for procurement according to Board Decision on Additional Support for Country Responses to COVID-19 (GF/B42/EDP11) The following emergency procedures established by WHO and the Regulatory Authorities of the Founding Members of the GHTF have been identified by the QA Team and will be used to determine eligibility for procurement of COVID-19 diagnostics. The product, to be considered as eligible for procurement with GF resources, shall be listed in one of the below mentioned lists: - WHO Prequalification decisions made as per the Emergency Use Listing (EUL) procedure opened to candidate in vitro diagnostics (IVDs) to detect SARS-CoV-2; - The United States Food and Drug Administration’s (USFDA) general recommendations and procedures applicable to the authorization of the emergency use of certain medical products under sections 564, 564A, and 564B of the Federal Food, Drug, and Cosmetic Act; - The decisions taken based on the Canada’s Minister of Health interim order (IO) to expedite the review of these medical devices, including test kits used to diagnose COVID-19; - The COVID-19 diagnostic tests approved by the Therapeutic Goods Administration (TGA) for inclusion on the Australian Register of Therapeutic Goods (ARTG) on the basis of the Expedited TGA assessment - The COVID-19 diagnostic tests approved by the Ministry of Health, Labour and Welfare after March 2020 with prior scientific review by the PMDA - The COVID-19 diagnostic tests listed on the French
    [Show full text]
  • The Science Commons in Health Research: Structure, Function, and Value
    J Technol Transfer (2007) 32:133–156 DOI 10.1007/s10961-006-9016-9 The science commons in health research: structure, function, and value Robert Cook-Deegan Published online: 7 December 2006 Ó Springer Science+Business Media, LLC 2006 Abstract The ‘‘science commons,’’ knowledge that is widely accessible at low or no cost, is a uniquely important input to scientific advance and cumulative technological innovation. It is primarily, although not exclusively, funded by government and nonprofit sources. Much of it is produced at academic research centers, although some academic science is proprietary and some privately funded R&D enters the science commons. Science in general aspires to Mertonian norms of openness, universality, objectivity, and critical inquiry. The science commons diverges from proprietary science primarily in being open and being very broadly available. These features make the science commons particularly valuable for advancing knowledge, for training innovators who will ultimately work in both public and private sectors, and in providing a common stock of knowledge upon which all players—both public and private—can draw readily. Open science plays two important roles that pro- prietary R&D cannot: it enables practical benefits even in the absence of profitable markets for goods and services, and its lays a shared foundation for subsequent private R&D. The history of genomics in the period 1992–2004, covering two periods when genomic startup firms attracted significant private R&D investment, illustrates these features of how a science commons contributes value. Commercial interest in genomics was intense during this period. Fierce competition between private sector and public sector genomics programs was highly visible.
    [Show full text]
  • Rutgers Clinical Genomics Laboratory Taqpath SARS-Cov-2 Assay EUA Summary
    Rutgers Clinical Genomics Laboratory TaqPath SARS-CoV-2 Assay EUA Summary ACCELERATED EMERGENCY USE AUTHORIZATION (EUA) SUMMARY SARS-CoV-2 ASSAY (Rutgers Clinical Genomics Laboratory) For in vitro diagnostic use Rx only For use under Emergency Use Authorization (EUA) Only (The Rutgers Clinical Genomics Laboratory TaqPath SARS-CoV-2 Assay will be performed in the Rutgers Clinical Genomics Laboratory, a Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a certified high-complexity laboratory, per the Instructions for Use that were reviewed by the FDA under this EUA). INTENDED USE The Rutgers Clinical Genomics Laboratory TaqPath SARS-CoV-2 Assay is a real-time reverse transcription polymerase chain reaction (rRT-PCR) test intended for the qualitative detection of nucleic acid from SARS-CoV-2 in oropharyngeal (throat) swab, nasopharyngeal swab, anterior nasal swab, mid-turbinate nasal swab, and bronchoalveolar lavage (BAL) fluid from individuals suspected of COVID-19 by their healthcare provider. This test is also for use with saliva specimens that are self-collected at home or in a healthcare setting by individuals using the Spectrum Solutions LLC SDNA-1000 Saliva Collection Device when determined to be appropriate by a healthcare provider. Testing is limited to Rutgers Clinical Genomics Laboratory (RCGL) at RUCDR Infinite Biologics – Rutgers University, Piscataway, NJ, that is a Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a certified high-complexity laboratory. Results are for the detection and identification of SARS-CoV-2 RNA. The SARS- CoV-2 RNA is generally detectable in respiratory specimens during the acute phase of infection.
    [Show full text]
  • Guide to Biotechnology 2008
    guide to biotechnology 2008 research & development health bioethics innovate industrial & environmental food & agriculture biodefense Biotechnology Industry Organization 1201 Maryland Avenue, SW imagine Suite 900 Washington, DC 20024 intellectual property 202.962.9200 (phone) 202.488.6301 (fax) bio.org inform bio.org The Guide to Biotechnology is compiled by the Biotechnology Industry Organization (BIO) Editors Roxanna Guilford-Blake Debbie Strickland Contributors BIO Staff table of Contents Biotechnology: A Collection of Technologies 1 Regenerative Medicine ................................................. 36 What Is Biotechnology? .................................................. 1 Vaccines ....................................................................... 37 Cells and Biological Molecules ........................................ 1 Plant-Made Pharmaceuticals ........................................ 37 Therapeutic Development Overview .............................. 38 Biotechnology Industry Facts 2 Market Capitalization, 1994–2006 .................................. 3 Agricultural Production Applications 41 U.S. Biotech Industry Statistics: 1995–2006 ................... 3 Crop Biotechnology ...................................................... 41 U.S. Public Companies by Region, 2006 ........................ 4 Forest Biotechnology .................................................... 44 Total Financing, 1998–2007 (in billions of U.S. dollars) .... 4 Animal Biotechnology ................................................... 45 Biotech
    [Show full text]
  • DNA Fragment Analysis by Capillary Electrophoresis User Guide
    USER GUIDE DNA Fragment Analysis by Capillary Electrophoresis Publication Number 4474504 Revision B For Research Use Only. Not intended for use in diagnostic procedures. For Research Use Only. Not intended for use in diagnostic procedures. The information in this guide is subject to change without notice. DISCLAIMER LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. TRADEMARKS All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. AmpErase, AmpliTaq, AmpliTaq Gold, and TaqMan are registered trademarks of Roche Molecular Systems, Inc. AFLP is a registered trademark of Keygene N.V. Millipore is a registered trademark of Merck KGaA. © 2014 Thermo Fisher Scientific Inc. All rights reserved. Contents About This Guide . 13 Revision history . 13 Purpose . 13 Prerequisites . 13 Structure of this guide . 14 ■ CHAPTER 1 Introduction to Fragment Analysis . 15 Fragment analysis versus sequencing…what is the difference? . 15 Fragment analysis . 15 Sequencing . 16 What can I do with fragment analysis? . 16 Types of applications . 16 Applications described in this guide . 17 What is capillary electrophoresis? . 18 Fragment analysis workflow . 19 ■ CHAPTER 2 Experimental Design .
    [Show full text]
  • Supporting Information Supplementary Methods
    Supporting Information Supplementary methods This appendix was part of the submitted manuscript and has been peer reviewed. It is posted as supplied by the authors. Appendix to: Caly L, Druce J, Roberts J, et al. Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia. Med J Aust 2020; doi: 10.5694/mja2.50569. Supplementary methods 1.1 Generation of SARS-CoV-2 cDNA 200μL aliquots from swab (nasopharyngeal in VTM), sputum, urine, faeces and serum samples were subjected to RNA extraction using the QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany) and eluted in 60μL. Reverse transcription was performed using the BioLine SensiFAST cDNA kit (Bioline, London, United Kingdom), total reaction mixture (20μL), containing 10μL RNA extract, 4μL 5x TransAmp buffer, 1μL reverse transcriptase and 5μL nuclease-free water. The reactions were incubated at 25°C for 10 min, 42°C for 15 min and 85°C for 5 min. 1.2 Nested SARS-CoV-2 RT-PCR and Sanger sequencing A PCR mixture containing 2μL cDNA, 1.6 μl 25mM MgCl2, 4μL 10x Qiagen Taq Buffer, 0.4μL 20mM dNTPs, 0.3μL Taq polymerase (Qiagen, Hilden, Germany) and 2μL of 10 μM primer pools as described2. Briefly, first round included the forward (5'-GGKTGGGAYTAYCCKAARTG-3') and reverse (5'-GGKTGGGAYTAYCCKAARTG-3') primers. Cycling conditions were 94°C for 10min, followed by 30 cycles of 94°C for 30s, 48°C for 30s and 72°C for 40s, with a final extension of 72°C for 10 min.
    [Show full text]
  • Applied Biosystems 3130 and 3130Xl Genetic Analyzers
    System Profile Applied Biosystems 3130 and 3130xl Genetic Analyzers. System Profile Applied Biosystems 3130 and 3130xl Genetic Analyzers Table of Contents A Powerful Blend of Flexibility and Performance 1 Ease-of-Use 1 Key Features 2 Capillary Electrophoresis 2 Automated Polymer Delivery System 2 Enhanced Thermal Control 2 High-Perfomance Capillaries and Electro-osmotic Flow Suppression (EOF) Polymers 2 Detection Method Designed for Sensitivity 3 Spectral Array Detection 3 Application Flexibility 4 Complete System Optimized for Multiple Applications 4 One Polymer, One Array, Maximum Performance 4 System Software Suite 5 Results 6 Summary 9 References 10 www.appliedbiosystems.com 16-capillary 3130xl Genetic Analyzer: High-performance workhorse. With 16-capillary throughput and advanced automation capabilities, the 3130xl system is flexible enough to meet the throughput needs of the busiest core facility or research group. The streamlined set-up and 24-hour unattended operation make it an ideal choice for low or medium throughput laboratories. Ease-of-Use Complete automation. At every scale, Applied Biosystems genetic analyzers are known for their advanced automation and “hands-free” operation. The Automated Polymer Delivery System A Powerful Blend of Flexibility and Performance eliminates manual washing and filling of polymer syringes, Applied Biosystems has a long tradition of providing significantly reducing the time required for instrument set- excellence in life science instruments, reagents, and software. up and maintenance. All steps are automated, including This tradition of pioneering and innovation in the field polymer loading, sample injection, separation and detection, of genetic analysis continues with the introduction of and data analysis. After placing plates on the autosampler Applied Biosystems next-generation systems, the 3130 and importing sample information, just select the “Start and 3130xl Genetic Analyzers.
    [Show full text]