Research Article 121 The chromatin remodeling factor Chd1l is required in the preimplantation embryo Alyssa C. Snider1, Denise Leong2, Q. Tian Wang1,3, Joanna Wysocka1,4, Mylene W. M. Yao2 and Matthew P. Scott1,5,* 1Departments of Developmental Biology, Genetics, and Bioengineering, University School of Medicine, Stanford, CA 94305-5101, USA 2Department of Obstetrics and Gynecology, University School of Medicine, Stanford, CA 94305-5101, USA 3Department of Biological Sciences, University of Illinois, Chicago, IL 60607, USA 4Department of Chemical and Systems Biology, University School of Medicine, Stanford, CA 94305-5101, USA 5Howard Hughes Medical Institute, Clark Center West W252, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5439, USA *Author for correspondence (
[email protected]) Biology Open 2, 121–131 doi: 10.1242/bio.20122949 Received 27th August 2012 Accepted 17th October 2012 Summary During preimplantation development, the embryo must important function in vivo, Chd1l is non-essential for establish totipotency and enact the earliest differentiation cultured ES cell survival, pluripotency, or differentiation, choices, processes that involve extensive chromatin suggesting that Chd1l is vital for events in embryos that are modification. To identify novel developmental regulators, distinct from events in ES cells. Our data reveal a novel role we screened for genes that are preferentially transcribed in for the chromatin remodeling factor Chd1l in the earliest the pluripotent inner cell mass (ICM) of the mouse cell divisions of mammalian development. blastocyst. Genes that encode chromatin remodeling factors were prominently represented in the ICM, ß 2012. Published by The Company of Biologists Ltd. This is including Chd1l, a member of the Snf2 gene family.