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DHTKD1 (Q-17): Sc-242577 SAN TA C RUZ BI OTEC HNOL OG Y, INC . DHTKD1 (Q-17): sc-242577 BACKGROUND APPLICATIONS The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion DHTKD1 (Q-17) is recommended for detection of DHTKD1 of mouse, rat and of 2-oxoglutarate to succinyl-CoA and CO 2. The complex contains multiple human origin by Western Blotting (starting dilution 1:200, dilution range copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution DHTKD1 (probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution mitochondrial), also known as KIAA1630 or dehydrogenase E1 and transke - range 1:30-1:3000). tolase domain-containing protein 1, is a 919 amino acid protein belonging to DHTKD1 (Q-17) is also recommended for detection of DHTKD1 in additional the -ketoglutarate dehydrogenase family. Thiamine pyrophosphate serves as α species, including equine, canine, bovine and avian. the cofactor for DHKTD1, which is localized to the mitochondrion. The gene encoding DHTKD1 maps to human chromosome 10p14 and mouse chromo - Suitable for use as control antibody for DHTKD1 siRNA (h): sc-90728, some 2 A1. DHTKD1 siRNA (m): sc-143036, DHTKD1 shRNA Plasmid (h): sc-90728-SH, DHTKD1 shRNA Plasmid (m): sc-143036-SH, DHTKD1 shRNA (h) Lentiviral REFERENCES Particles: sc-90728-V and DHTKD1 shRNA (m) Lentiviral Particles: sc-143036- V. 1. Rice, J.E., et al. 1991. Evidence for a protein X-like domain at the N-termi - nus of the E1 component of the mammalian 2-oxoglutarate dehydrogenase Molecular Weight of DHTKD1: 103 kDa. complex. Biochem. Soc. Trans. 19: 403S. Positive Controls: mouse liver extract: sc-2256 or HeLa whole cell lysate: 2. Fukushima, N., et al. 1995. Establishment and structural analysis of human sc- 2200. mAb to the E2 component of the 2-oxoglutarate dehydrogenase complex generated from a patient with primary biliary cirrhosis. Int. Immunol. 7: RECOMMENDED SECONDARY REAGENTS 1047-1055. To ensure optimal results, the following support (secondary) reagents are 3. Nagase, T., et al. 2000. Prediction of the coding sequences of unidentified recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 human genes. XVIII. The complete sequences of 100 new cDNA clones (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey from brain which code for large proteins in vitro. DNA Res. 7: 273-281. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 4. Surendran, S., et al. 2002. DOOR syndrome: deficiency of E1 component sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- of the 2-oxoglutarate dehydrogenase complex. Am. J. Med. Genet. 113: itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 371-374. 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution 5. Hiromasa, Y., et al. 2004. Organization of the cores of the mammalian range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. protein and their capacities to bind the E1 and E3 components. J. Biol. Chem. 279: 6921-6933. DATA CHROMOSOMAL LOCATION Genetic locus: DHTKD1 (human) mapping to 10p14; Dhtkd1 (mouse) mapping 132 K – < DHTKD1 132 K – to 2 A1. 90K– < DHTKD1 90K– SOURCE 55K– DHTKD1 (Q-17) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of DHTKD1 of human origin. DHTKD1 (Q-17): sc-242577. Western blot analysis of DHTKD1 (Q-17): sc-242577. Western blot analysis of PRODUCT DHTKD1 expression in mouse liver tissue extract. DHTKD1 expression in HeLa whole cell lysate. Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide RESEARCH USE and 0.1% gelatin. For research use only, not for use in diagnostic procedures. Blocking peptide available for competition studies, sc-242577 P, (100 µg pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). PROTOCOLS STORAGE See our web site at www.scbt.com or our catalog for detailed protocols and support products. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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