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Prussian Blue - Eosin Staining Kit Prussian blue - Eosin staining kit Cat.No.：E670108 Package：100 Test /500 Test Description Hemoglobin (hemosiderin) is a hemoglobin-derived pigment, golden yellow or brown particles, because of its iron, golden yellow, it is called hemosiderin. When red blood cells are phagocytosed by macrophages, hemoglobin is broken down into iron-free orange blood and iron-containing hemosiderin under the action of lysosomal enzymes. Perls Prussian blue reaction (Prussian blue reaction), also known as hemosiderin staining, that is, after treatment of potassium ferrocyanide and dilute acid can produce blue, common in phagocytes or interstitial, the main display of ferric salt. Perls Prussian blue is a very classical histochemical reaction that is a sensitive, traditional and excellent method of displaying ferric iron in tissues. The staining principle is that potassium ferrocyanide makes the ferric ion from the protein diluted with hydrochloric acid Isolated, ferric iron and potassium ferrocyanide reaction to generate a blue insoluble compound that ferric ferricyanide Prussian blue, so the reaction is called Prussian blue reaction. Ferrous iron ferrocyanide is a very stable compound, after the reaction can be red dye staining, such as nuclear red, eosin, such as neutral red. Prussian blue staining reagents commonly used to show a variety of hemorrhagic lesions within the local tissue, common in phagocytes. In determining the hemosiderin deposition, Perls reaction can be confirmed, the staining method can be a good distinction between hemosiderin and other pigments. The dyeing liquid has good stability, can be stored for a long time, is not easy to produce precipitation, has a wide range of applications, can be counterstained. Prussian blue - eosin staining kit, the dye solution using eosin staining solution, is also commonly used in the dye solution, the dye solution staining time than the nuclear solid red stain to be shorter. Storage Store at room temperaturefor 12months. Kit Components Component 100 Test 500 Test Component A 10% Potassium 5 mL 25 mL ferrocyanide 5 mL 25 mL Component B 20% hydrochloric 10 mL 50 mL acid Component C 0.25% Eosin staining solution Protocol 1 1 Procedure Sangon Biotech 1. Slice placed in drying oven at 60 ° C for 1 h。 2. Dewaxing：XyleneⅠ15 min → XyleneⅡ15 min → ethyl alcohol absoluteⅠ5 min → ethyl alcohol absoluteⅡ5 min → 95% alcohol 5 min → 85% alcohol 5 min → 75% alcohol 5 min → Deionized water 3. Prussian blue staining solution: before use, 10% potassium ferrocyanide and 20% hydrochloric acid mixed by 1: 1, Prussian blue staining solution shall be used immediately. 4. Prussian blue working solution staining at room temperature for 15-30 min. 5. Immersion in distilled water 5 min × 3 times. 6. 0.25% Eosin staining solution light staining at room temperature for15-30 s。 7. Tap water rinse for 1 ~ 5 s。 8. Dehydration, transparent, neutral gum seal, observed under a microscope。 Dyeing results Prussian blue - Eosin staining solution for lymphoma tissue staining 40 × Sangonferric ion····························· BiLake Blueo tech Cytoplasm·························red Precautions 1. tissue avoid the use of acidic fixatives and chromate treatment can interfere with the preservation of iron.The entire operation of the container should be clean, to avoid the use of metal products。 2. Wash slices and containers using distilled water, because tap water contains iron。 3. Prussian blue working fluid can not be prepared in advance. 4. Prussian blue staining time adjusted according to the sample. 5. For your safety and health, wear lab coats and disposable gloves. 6. This product is only used for scientific research experiment, can not be used for medical and clinical diagnosis. Sangon Biotech .
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