Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 5 September 2020 doi:10.20944/preprints202009.0108.v1 1 Article 2 Systematic Surveys of Iron Homeostasis Mechanisms 3 reveal Ferritin Superfamily and Nucleotide Surveillance 4 Regulation to be modified by PINK1 Absence 5 Jana Key 1,2, Nesli Ece Sen 1,2, Aleksandar Arsovic 1, Stella Krämer 1, Robert Hülse 1, Natasha 6 Nadeem Khan 1, David Meierhofer 3, Suzana Gispert 1, Gabriele Koepf 1, and Georg Auburger 1,* 7 1 Experimental Neurology, Goethe University Medical School, 60590 Frankfurt am Main, Germany 8 2 Faculty of Biosciences, Goethe-University, Altenhöferallee 1, 60438 Frankfurt am Main, Germany 9 3 Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany 10 * Correspondence:
[email protected] 11 12 Abstract: Iron deprivation activates mitophagy and extends lifespan in nematodes. In patients 13 suffering from Parkinson’s disease (PD), PINK1-PRKN mutations via deficient mitophagy trigger 14 iron accumulation and reduce lifespan. To evaluate molecular effects of iron chelator drugs as a 15 potential PD therapy, we assessed fibroblasts by global proteome profiles and targeted transcript 16 analyses. In mouse cells, iron shortage decreased protein abundance for iron-binding nucleotide 17 metabolism enzymes (prominently XDH and ferritin homolog RRM2). It also decreased the 18 expression of factors with a role for nucleotide surveillance, which associate with iron-sulfur- 19 clusters (ISC), and are important for growth and survival. This widespread effect included 20 prominently Nthl1-Ppat-Bdh2, but also mitochondrial Glrx5-Nfu1-Bola1, cytosolic Aco1-Abce1-Tyw5, 21 and nuclear Dna2-Elp3-Pold1-Prim2.