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Custom Peptide Synthesis Standard | Modified | Heavy Peptides | Libraries 2 Contents Custom peptide synthesis Standard | modified | heavy peptides | libraries 2 Contents Custom peptide synthesis 4 An overview 4 Standard peptide synthesis service 6 PEPotec Immuno Peptide Libraries 7 Targeted quantitation 8 HeavyPeptide and LightPeptide AQUA standards 9 PEPotec SRM Peptide Libraries 11 3 Custom peptide synthesis An overview Optimizing the efficiency of peptide synthesis can be challenging. At Thermo Fisher Scientific, we have the experience, equipment, and knowledge to meet your needs for custom peptide synthesis. Our synthesis team based on your input. Our experienced peptide scientists has accumulated significant expertise through successfully will support you with peptide sequence, scale, and/or producing tens of thousands of custom peptides. We are purity selections for your assay to help you achieve the constantly adapting our product offerings to your needs best results for your application (Table 1). Custom peptide synthesis capabilities Table 1. Summary of peptide services and specifications. HeavyPeptide and PEPotec immuno PEPotec SRM LightPeptide AQUA Standard peptides peptide libraries peptide libraries Standards Primary Basic research or biomarker Epitope mapping, vaccine Quantitative mass Targeted proteomics application discovery development spectrometry Over 400 modifications High quality; accurate and Key features High throughput, low toxicity High throughput available precise concentration Peptide length* 2–110 amino acids 6–20 amino acids 6–25 amino acids Up to 15 amino acids Manufacturing 1 mg–1 g ≥1 mg ≥0.1 mg Multiple scales available scale** Crude (as synthesized) or Purity Crude (>50%) to >98% Crude (as synthesized) >95% to >97% >70% MALDI-TOF MS check or Quality control MALDI-TOF MS and HPLC MALDI-TOF MS analysis; HPLC for purified MALDI-TOF MS, HPLC, AAA grade 2–3 weeks AQUA Express, Production time 2–3 weeks 4 weeks 2–3 weeks 5–7 weeks standard AQUA * Please inquire about longer peptide lengths. ** Greater amounts available upon request. Quality control Delivery format Quality control for our peptide services includes analyses Our peptides are provided in lyophilized and liquid formats using mass spectrometry (MS) for peptide identification, in glass vials as well as in 96-well Thermo Scientific™ analytical high-performance liquid chromatography (HPLC) Matrix™ tube plates, depending on the product for peptide purity, and amino acid analysis (AAA) for category. Please inquire for additional formats and other peptide concentration. Additional nonstandard analyses are custom requests. available on request. Analysis results are reported on the Certificate of Analysis for your records. 4 Professional support Peptide applications We have experienced and dedicated staff to assist you The use of synthetic peptides has significantly impacted through peptide selection, ordering, tracking, and delivery many areas of research. Our portfolio of custom peptide processes. We realize that the process can be lengthy, and services supports a variety of end applications, and we we strive to keep you informed every step of the way. strive to provide high-quality, fit-for-purpose peptides to enable success. Peptide services Custom peptides Peptide libraries Heavy peptides Unmodified PEPotec Immuno Peptide Libraries HeavyPeptide AQUA Modified PEPotec SRM Peptide Libraries LightPeptide AQUA 13 15 R( C6 N4) Alexa Fluor dye label R Applications Protein interactions Quality control testing Assay development Quantitative proteomics ELISA, enzymatic, etc. SRM, PRM, SureQuant Assays Epitope mapping Structural studies 5 Standard peptide synthesis service High-quality peptides from validated Modifications process tailored to help meet your needs We offer a wide range of N-terminal, C-terminal, and other The Thermo Scientific™ Standard Peptide modifications with our standard peptide service (Table 3). Synthesis Service offers numerous options Please go to thermofisher.com/peptides for the most up- for peptide purity, modifications, and formats, to-date list and available positions in the peptide sequence. providing you the flexibility to meet your research needs. Custom conjugation services are also available, including conjugation to carrier proteins (KLH, BSA, OVA, or Thermo Our peptide synthesis utilizes the latest Fmoc Scientific™ Imject™ Blue Carrier™ Protein) and MAPs. solid-phase technology and reversed-phase HPLC for Table 3. Peptide modifications offered with standard peptide purification. Peptide sequence is confirmed by MS, peptide synthesis. and peptide purity is determined using analytical HPLC. • Acetylation of N terminus (Ac-NH–) • Fluorescein (FITC/5-FAM) • Acetyl-lysine • Hydrocarbon spacers Highlights • Aldehyde • Hydroxy proline • High quality—all peptides are synthesized with • Invitrogen™ Alexa Fluor™ dyes • Hydroxy tryptophan high-quality materials • 6-amino hexanoic acid (Ahx) • Isotopically labeled amino acids 13 15 • 6-amino caproic acid (Aca) (with C, N) • Modifications—most comprehensive list of available • Mercaptopropionic acid • Amidation of C terminus (–CONH2) modifications and labels • Amino benzoic acid • Methoxy-coumarin-acetic acid (MCA) • Beta-alanine • Validated—all peptides are analyzed by MS alone or in • Methionine sulfone • Biotin combination with analytical HPLC • Methionine sulfoxide [Met(O)] • Carbamido methylation (CAM) • Monomethyl lysine • Citrulline • Flexible—peptides available in a variety of formats and • Monomethyl arginine • Conjugation to proteins (BSA, KLH) purities; manual synthesis available for difficult peptides • Multiple antigen peptides (MAPs) • Conjugation to oligonucleotides • Norleucine (Nle) • Custom FRET peptides Table 2. Specifications for standard grade peptides. • Phosphorylation of serine, • Cyclization via termini or disulfide threonine, and tyrosine bridge • Polyethylene glycol (PEG) spacer Peptide length* 2–110 amino acids, L or D isoforms • D amino acids • Pyroglutamic acid (Pyr) Manufacturing 1 mg –1 g • Dabcyl scale** • Rhodamine • Dabsyl Purity Crude (>50%) to >98% pure • Special amino acids (D amino • Dansyl acids, other amino acids) Counterion TFA • Dihydroxy tyrosine • Tetramethylrhodamine (TAMRA) Formulation Lyophilized • Dimethyl lysine • Invitrogen™ Texas Red™ dye Delivery format Glass vial • Dinitrophenyl (DNP) • Ubiquitination Production time Standard delivery within 2–3 weeks • EDANS • Other dyes or modifications on Shipment Lyophilized, room temperature • Farnesyl request MALDI-TOF MS for crude peptides; MALDI-TOF MS and Quality control HPLC for all other purity grades Extensive list of modifications and labels, including Table 4. Peptide purity recommendations Modifications heavy peptides (isotope-labeled) by application. Custom conjugation available, e.g., protein-peptide Conjugation conjugates and multiple antigen peptides (MAPs) Alternate counterion, AAA, endotoxin testing, Crude: peptides for high-throughput screening Optional services solubility testing >70%: antigens for polyclonal antibody production * Please inquire about longer peptide lengths. >75%: ligands for affinity purification, enzyme substrate studies, epitope mapping ** Greater amounts available upon request. >80%: qualitative peptide blocking studies, protein electrophoresis applications >85%: immunological applications, peptide arrays, affinity purification >90%: qualitative bioassays, monoclonal antibody production >95%: quantitative bioassays (e.g., ELISA, enzymology, biological activity) >97%: MS proteomics applications >98%: structural studies, crystallography, NMR, in vivo applications thermofisher.com/standardpeptide Note: Listed purity levels indicate the minimum typical requirements for each application. 6 PEPotec Immuno Peptide Libraries Ideal for epitope mapping and high-throughput Thermo Scientific™ PEPotec™ Immuno Peptide Libraries are screening for immunology applications fully synthetic custom libraries that support high-throughput screening assays to map epitopes or identify immunogenic sites in proteins. These libraries are fully customizable and supplied with acetate as the counterion to avoid potential toxicity issues in cell-based applications. Table 5. PEPotec Immuno Peptide Library service. Peptide length* 6–20 amino acids; L isoforms only Quantity ≥1 mg Purity** Crude (as synthesized) C-terminal residue Any unmodified L isoform amino acid Counterion Acetate Formulation Lyophilized Peptides are provided in individual 2D barcoded tubes in Delivery format Highlights Matrix 96-tube plates • Low toxicity—acetate used as the counterion to reduce Production time 4 weeks toxicity when compared to trifluoroacetate (TFA) [1] Shipment Lyophilized, room temperature Minimum order 4 peptides; surcharge for orders of <48 peptides • Convenient—lyophilized peptides provided in individual Quality control*** MALDI-TOF MS 2D barcoded tubes in 96-tube plates Acetylated Lys, carbamidomethylated Cys, Modifications phosphorylation, etc. • Flexible—optional services, including phosphorylation Optional services Solubilization in DMSO, individual tube labeling, etc. and acetylation, are available for studying signaling and * Please inquire about longer peptide lengths. ** No purification given, as peptide purity is sequence-dependent and a function of different liquid regulatory proteins chromatographic conditions. *** For all peptides except phosphopeptides, the major peak in the MS analysis represents the target Applications of interest. Peptides must pass both the MS analysis and the final gross weight criteria (>1 mg) before shipment. If peptides do not pass
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