Nck2 (I364) Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. Nck2 (I364) polyclonal antibody Catalog: BS1455 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: by affinity-chromatography using epitope-specific im- The NCK family of SH2/SH3 adaptor proteins consists of munogen and the purity is > 95% (by SDS-PAGE). two members, NCK1 (NCKα) and NCK2 (NCKβ), which Applications: couple tyrosine kinase signaling, including the EGF and IHC: 1:50~1:200 PDGF receptor-pathways, to downstream signaling pro- Storage&Stability: teins. Specifically, overexpression of NCK1 in NIH/3T3 Store at 4°C short term. Aliquot and store at -20°C long cells decreases DNA synthesis stimulated by EGF. Fur- term. Avoid freeze-thaw cycles. thermore, the SH2 domain of NCK2 inhibits EGF- and Specificity: PDGF-induced DNA synthesis. The SH3 domain of NCK NCK2 (I364) polyclonal antibody detects endogenous binds a proline-rich domain on PAK, a known actin cyto- levels of NCK2 protein. skeleton regulator. The NCK protein thus mediates the DATA: interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphory- late mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, re- spectively. Product: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraf- Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, fin-embedded human colon carcinoma tissue. 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. ~ 43 kDa Swiss-Prot: O43639 Purification&Purity: The antibody was affinity-purified from rabbit antiserum Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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LIM Domain Proteins Pinch1/2 Regulate Chondrogenesis and Bone Mass in Mice
Bone Research www.nature.com/boneres ARTICLE OPEN LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice Yiming Lei1, Xuekun Fu1, Pengyu Li1, Sixiong Lin1,2, Qinnan Yan1, Yumei Lai3, Xin Liu1, Yishu Wang1, Xiaochun Bai 4, Chuanju Liu5,6, Di Chen7, Xuenong Zou2, Xu Cao8, Huiling Cao1 and Guozhi Xiao1 The LIM domain-containing proteins Pinch1/2 regulate integrin activation and cell–extracellular matrix interaction and adhesion. Here, we report that deleting Pinch1 in limb mesenchymal stem cells (MSCs) and Pinch2 globally (double knockout; dKO) in mice causes severe chondrodysplasia, while single mutant mice do not display marked defects. Pinch deletion decreases chondrocyte proliferation, accelerates cell differentiation and disrupts column formation. Pinch loss drastically reduces Smad2/3 protein expression in proliferative zone (PZ) chondrocytes and increases Runx2 and Col10a1 expression in both PZ and hypertrophic zone (HZ) chondrocytes. Pinch loss increases sclerostin and Rankl expression in HZ chondrocytes, reduces bone formation, and increases bone resorption, leading to low bone mass. In vitro studies revealed that Pinch1 and Smad2/3 colocalize in the nuclei of chondrocytes. Through its C-terminal region, Pinch1 interacts with Smad2/3 proteins. Pinch loss increases Smad2/3 ubiquitination and degradation in primary bone marrow stromal cells (BMSCs). Pinch loss reduces TGF-β-induced Smad2/3 phosphorylation and nuclear localization in primary BMSCs. Interestingly, compared to those from single mutant mice, BMSCs from dKO mice express dramatically lower protein levels of β-catenin and Yap1/Taz and display reduced osteogenic but increased adipogenic differentiation capacity. Finally, ablating Pinch1 in chondrocytes and Pinch2 globally causes severe osteopenia with subtle limb ﬁ 1234567890();,: shortening.
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UNIVERSITY of CALIFORNIA RIVERSIDE Investigations Into The
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Anti-NCK2 Antibody (ARG59810)
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