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Nck2 (I364) polyclonal antibody

Catalog: BS1455 Host: Rabbit Reactivity: Human,Mouse,Rat

BackGround: by affinity-chromatography using epitope-specific im- The NCK family of SH2/SH3 adaptor consists of munogen and the purity is > 95% (by SDS-PAGE). two members, NCK1 (NCKα) and NCK2 (NCKβ), which Applications: couple tyrosine kinase signaling, including the EGF and IHC: 1:50~1:200 PDGF receptor-pathways, to downstream signaling pro- Storage&Stability: teins. Specifically, overexpression of NCK1 in NIH/3T3 Store at 4°C short term. Aliquot and store at -20°C long cells decreases DNA synthesis stimulated by EGF. Fur- term. Avoid freeze-thaw cycles. thermore, the SH2 domain of NCK2 inhibits EGF- and Specificity: PDGF-induced DNA synthesis. The SH3 domain of NCK NCK2 (I364) polyclonal antibody detects endogenous binds a proline-rich domain on PAK, a known actin cyto- levels of NCK2 . skeleton regulator. The NCK protein thus mediates the DATA: interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphory- late mammalian NCK1 and NCK2.The human NCK1 and NCK2 map to 3q21 and 2q12, re- spectively. Product: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraf- Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, fin-embedded human colon carcinoma tissue. 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. ~ 43 kDa Swiss-Prot: O43639 Purification&Purity: The antibody was affinity-purified from rabbit antiserum

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