Nck2 (I364) Polyclonal Antibody

PRODUCT DATA SHEET Bioworld Technology,Inc. Nck2 (I364) polyclonal antibody Catalog: BS1455 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: by affinity-chromatography using epitope-specific im- The NCK family of SH2/SH3 adaptor proteins consists of munogen and the purity is > 95% (by SDS-PAGE). two members, NCK1 (NCKα) and NCK2 (NCKβ), which Applications: couple tyrosine kinase signaling, including the EGF and IHC: 1:50~1:200 PDGF receptor-pathways, to downstream signaling pro- Storage&Stability: teins. Specifically, overexpression of NCK1 in NIH/3T3 Store at 4°C short term. Aliquot and store at -20°C long cells decreases DNA synthesis stimulated by EGF. Fur- term. Avoid freeze-thaw cycles. thermore, the SH2 domain of NCK2 inhibits EGF- and Specificity: PDGF-induced DNA synthesis. The SH3 domain of NCK NCK2 (I364) polyclonal antibody detects endogenous binds a proline-rich domain on PAK, a known actin cyto- levels of NCK2 protein. skeleton regulator. The NCK protein thus mediates the DATA: interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphory- late mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, re- spectively. Product: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraf- Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, fin-embedded human colon carcinoma tissue. 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. ~ 43 kDa Swiss-Prot: O43639 Purification&Purity: The antibody was affinity-purified from rabbit antiserum Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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LIM Domain Proteins Pinch1/2 Regulate Chondrogenesis and Bone Mass in Mice
Bone Research www.nature.com/boneres ARTICLE OPEN LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice Yiming Lei1, Xuekun Fu1, Pengyu Li1, Sixiong Lin1,2, Qinnan Yan1, Yumei Lai3, Xin Liu1, Yishu Wang1, Xiaochun Bai 4, Chuanju Liu5,6, Di Chen7, Xuenong Zou2, Xu Cao8, Huiling Cao1 and Guozhi Xiao1 The LIM domain-containing proteins Pinch1/2 regulate integrin activation and cell–extracellular matrix interaction and adhesion. Here, we report that deleting Pinch1 in limb mesenchymal stem cells (MSCs) and Pinch2 globally (double knockout; dKO) in mice causes severe chondrodysplasia, while single mutant mice do not display marked defects. Pinch deletion decreases chondrocyte proliferation, accelerates cell differentiation and disrupts column formation. Pinch loss drastically reduces Smad2/3 protein expression in proliferative zone (PZ) chondrocytes and increases Runx2 and Col10a1 expression in both PZ and hypertrophic zone (HZ) chondrocytes. Pinch loss increases sclerostin and Rankl expression in HZ chondrocytes, reduces bone formation, and increases bone resorption, leading to low bone mass. In vitro studies revealed that Pinch1 and Smad2/3 colocalize in the nuclei of chondrocytes. Through its C-terminal region, Pinch1 interacts with Smad2/3 proteins. Pinch loss increases Smad2/3 ubiquitination and degradation in primary bone marrow stromal cells (BMSCs). Pinch loss reduces TGF-β-induced Smad2/3 phosphorylation and nuclear localization in primary BMSCs. Interestingly, compared to those from single mutant mice, BMSCs from dKO mice express dramatically lower protein levels of β-catenin and Yap1/Taz and display reduced osteogenic but increased adipogenic differentiation capacity. Finally, ablating Pinch1 in chondrocytes and Pinch2 globally causes severe osteopenia with subtle limb ﬁ 1234567890();,: shortening.
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Targeting of the EGFR/Β1 Integrin Connecting Proteins PINCH1 and Nck2 Radiosensitizes Three-Dimensional SCC Cell Cultures
ONCOLOGY REPORTS 34: 469-476, 2015 Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures LYDIA ROSSOW1,2, IRIS EKE1,2, ELLEN DICKREuTER1,2 and NILS CORDES1-5 1OncoRay-National Center for Radiation Research in Oncology, Faculty of Medicine and university Hospital Carl Gustav Carus, Technische universität Dresden, D-01307 Dresden, and Helmholtz-Zentrum Dresden-Rossendorf, D-01328 Dresden; 2Department of Radiation Oncology, university Hospital Carl Gustav Carus, Technische universität Dresden, Dresden; 3Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiooncology, Dresden; 4German Cancer Consortium (DKTK), D-01307 Dresden; 5German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany Received March 31, 2015; Accepted May 4, 2015 DOI: 10.3892/or.2015.4006 Abstract. Epidermal growth factor receptor (EGFR) signaling Introduction plays an important role in tumor cell resistance to therapy. In addition to ligand binding, mutual and cooperative interac- Epidermal growth factor receptor (EGFR) signaling is known tions of EGFR with integrin cell adhesion receptors critically to be deregulated in many human tumors (1,2). Causative are influence proper downstream signaling through a number of EGFR gene amplifications and mutations resulting in receptor bridging adapter proteins. In the present study, we analyzed overexpression and constitutively active EGFR tyrosine the role of two of these adapter proteins, called PINCH1 kinase activation. Due to its substantial role in
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UNIVERSITY of CALIFORNIA RIVERSIDE Investigations Into The
UNIVERSITY OF CALIFORNIA RIVERSIDE Investigations into the Role of TAF1-mediated Phosphorylation in Gene Regulation A Dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Cell, Molecular and Developmental Biology by Brian James Gadd December 2012 Dissertation Committee: Dr. Xuan Liu, Chairperson Dr. Frank Sauer Dr. Frances M. Sladek Copyright by Brian James Gadd 2012 The Dissertation of Brian James Gadd is approved Committee Chairperson University of California, Riverside Acknowledgments I am thankful to Dr. Liu for her patience and support over the last eight years. I am deeply indebted to my committee members, Dr. Frank Sauer and Dr. Frances Sladek for the insightful comments on my research and this dissertation. Thanks goes out to CMDB, especially Dr. Bachant, Dr. Springer and Kathy Redd for their support. Thanks to all the members of the Liu lab both past and present. A very special thanks to the members of the Sauer lab, including Silvia, Stephane, David, Matt, Stephen, Ninuo, Toby, Josh, Alice, Alex and Flora. You have made all the years here ﬂy by and made them so enjoyable. From the Sladek lab I want to thank Eugene, John, Linh and Karthi. Special thanks go out to all the friends I’ve made over the years here. Chris, Amber, Stephane and David, thank you so much for feeding me, encouraging me and keeping me sane. Thanks to the brothers for all your encouragement and prayers. To any I haven’t mentioned by name, I promise I haven’t forgotten all you’ve done for me during my graduate years.
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Anti-NCK2 Antibody (ARG59810)
Product datasheet [email protected] ARG59810 Package: 100 μl anti-NCK2 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes NCK2 Tested Reactivity Hu, Ms, Rat Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name NCK2 Antigen Species Human Immunogen Recombinant fusion protein corresponding to aa. 1-380 of Human NCK2 (NP_003572.2). Conjugation Un-conjugated Alternate Names Nck-2; NCKbeta; NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta; GRB4; Growth factor receptor-bound protein 4; Cytoplasmic protein NCK2 Application Instructions Application table Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control Rat brain, Mouse lung and 293T Calculated Mw 43 kDa Observed Size 43 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol NCK2 Gene Full Name NCK adaptor protein 2 Background This gene encodes a member of the NCK family of adaptor proteins.
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Nck Adaptor Proteins Link Tks5 to Invadopodia Actin Regulation and ECM Degradation
Research Article 2727 Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation Stanley S. Stylli1, Stacey T. T. I1, Anne M. Verhagen2, San San Xu1, Ian Pass3, Sara A. Courtneidge3 and Peter Lock1,*,‡ 1Department of Surgery, University of Melbourne, Level 5, Clinical Sciences Building, Royal Melbourne Hospital, Parkville, Victoria 3052, Australia 2Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia 3Burnham Institute for Medical Research, Torrey Pines Road, La Jolla, CA 92037, USA *Present address: Biochemistry Department, La Trobe University, Victoria 3086, Australia ‡Author for correspondence (e-mail: [email protected]) Accepted 5 May 2009 Journal of Cell Science 122, 2727-2740 Published by The Company of Biologists 2009 doi:10.1242/jcs.046680 Summary Invadopodia are actin-based projections enriched with overexpression and inhibited by Nck1 depletion. We show that proteases, which invasive cancer cells use to degrade the clustering at the plasma membrane of the Tks5 inter-SH3 region extracellular matrix (ECM). The Phox homology (PX)-Src containing Y557 triggers phosphorylation at this site, facilitating homology (SH)3 domain adaptor protein Tks5 (also known as Nck recruitment and F-actin assembly. These results identify a SH3PXD2A) cooperates with Src tyrosine kinase to promote Src-Tks5-Nck pathway in ECM-degrading invadopodia that invadopodia formation but the underlying pathway is not clear. shows parallels with pathways linking several mammalian and Here we show that Src phosphorylates Tks5 at Y557, inducing pathogen-derived proteins to local actin regulation. it to associate directly with the SH3-SH2 domain adaptor proteins Nck1 and Nck2 in invadopodia.
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Consequences of Loss of PINCH2 Expression in Mice
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Rpcmp: Robust P-Value Combination by Multiple Partitions with Applications to ATAC-Seq Data Menglan Cai and Limin Li*
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CREB-Dependent Transcription in Astrocytes: Signalling Pathways, Gene Profiles and Neuroprotective Role in Brain Injury
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(NCK2) Rabbit Polyclonal Antibody – AP06442PU-N
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