PRODUCT DATA SHEET Bioworld Technology,Inc. Nck2 (I364) polyclonal antibody Catalog: BS1455 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: by affinity-chromatography using epitope-specific im- The NCK family of SH2/SH3 adaptor proteins consists of munogen and the purity is > 95% (by SDS-PAGE). two members, NCK1 (NCKα) and NCK2 (NCKβ), which Applications: couple tyrosine kinase signaling, including the EGF and IHC: 1:50~1:200 PDGF receptor-pathways, to downstream signaling pro- Storage&Stability: teins. Specifically, overexpression of NCK1 in NIH/3T3 Store at 4°C short term. Aliquot and store at -20°C long cells decreases DNA synthesis stimulated by EGF. Fur- term. Avoid freeze-thaw cycles. thermore, the SH2 domain of NCK2 inhibits EGF- and Specificity: PDGF-induced DNA synthesis. The SH3 domain of NCK NCK2 (I364) polyclonal antibody detects endogenous binds a proline-rich domain on PAK, a known actin cyto- levels of NCK2 protein. skeleton regulator. The NCK protein thus mediates the DATA: interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphory- late mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, re- spectively. Product: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraf- Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, fin-embedded human colon carcinoma tissue. 50% glycerol, pH7.2 Note: Molecular Weight: For research use only, not for use in diagnostic procedure. ~ 43 kDa Swiss-Prot: O43639 Purification&Purity: The antibody was affinity-purified from rabbit antiserum Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .