Agricultural Genetic Engineering Technology and Sustainable Development in the African Food Security Context
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Genetically Modified Cotton
BACKGROUND 2013 GENETICALLY MODIFIED COTTON Genetically engineered (also called genetically modified or GM) cotton is currently grown on 25 million hectares around the world, mostly in India, China, Pakistan and the US.1 Other countries growing much smaller amounts of GM cotton are South Africa, Burkina Faso, Sudan, Brazil, Argentina, Paraguay, Columbia, Mexico, Costa Rica, Burma, Australia, and Egypt. GM cotton is engineered with one of two traits. One makes it resistant to glyphosate-based herbicides such as Monsanto’s Roundup, SUMMARY while the other stimulates the plant to produce a toxin that kills the bollworm, one of the crop’s primary pests. This pest-resistant cotton GM COTTON FAILED is engineered with a gene from the bacteria Bacillus thurengiensis FARMERS IN INDIA or “Bt”, and is the more commonly grown of the two types. » Yields declined. BT COTTON IN INDIA » Secondary pests emerged, Cotton is an important cash crop in India. It is grown on 12 million forcing increased pesticide use. hectares, making India the second largest producer of cotton in the » The price of cotton seed rose. world, behind China. Insect-resistant Bt cotton is the only GM crop currently grown in India.2 It was introduced in India by Monsanto in » Farmers lost the option to buy 2002, under the trade name Bollgard, in a joint venture with the non-GM cotton seeds. Indian seed company Mahyco. Monsanto promised Indian farmers that Bt cotton would: 1. Reduce the amount of pesticides farmers need to buy to control pests, 2. Increase harvests and farm income by reducing crop losses due to pest attacks.3 In the first few years after Bt cotton was commercialized in India, some farmers saw reductions in pesticide use and crop losses, but this pattern quickly and dramatically changed. -
Improvement of Late Blight Resistance on Potato by Transgenesis and Cisgenesis
Improvement of late blight resistance on potato by transgenesis and cisgenesis Name: Tiantian Liu Registration number: 860927523040 Examiners: Prof. Evert Jacobsen & Dr. Jack Vossen Supervision: MSc Suxian Zhu & Dr. Jack Vossen Course code: PBR-80436 Thesis Plant Breeding Study specialisation: Plant Breeding and Genetic Resources Abstract This project aimed at the improvement of late blight resistance in potato by means of transgenesis and cisgenesis. In transgenesis, Rpi-mcq1 was successfully cloned into vector pBINPLUS. After single R gene transformation or by transformation of multiple different R genes, positive transformants were detected by PCR and functional assays in cisgenesis. Different transformation approaches were followed in this project in order to find out an efficient method for generating marker free plants. The results indicated that co-transformation of selection marker containing and marker free constructs is much more efficient in generating R gene positive plants than transformation of marker free constructs alone. However, several cisgenic plants were also selected from marker free transformation. Moreover, in vitro disease assay could be efficiently used as a pre-selection for positive R-gene containing regenerants. Keywords: potato; marker free; (co-)transformation; R-gene; functionality i Thesis Outline To improve the late blight resistance in potato by cisgenesis, three experiments were carried out. 1. To build up a Desiree transformants differential set, Rpi-mcq1 gene was cloned from vector pCLD04541 with the amplification primers which were designed by removing some nucleotides to reduce the length of gene from the patent (Jones et al., 2010). It was transferred into the vector pBINPLUS. 2. In order to get sufficient numbers of cisgenic plants, marker free transformation with two R genes, Rpi-sto1 and Rpi-blb3 , was carried out in previous experiments. -
Combatting Monsanto
Picture: Grassroots International Combatting Monsanto Grassroots resistance to the corporate power of agribusiness in the era of the ‘green economy’ and a changing climate La Via Campesina, Friends of the Earth International, Combat Monsanto Technical data name: “Combatting Monsanto Grassroots resistance to the corporate power of agribusiness in the era of the ‘green economy’ and a changing climate” author: Joseph Zacune ([email protected]) with contributions from activists around the world editing: Ronnie Hall ([email protected]) design and layout: Nicolás Medina – REDES-FoE Uruguay March 2012 Combatting Monsanto Grassroots resistance to the corporate power of agribusiness in the era of the ‘green economy’ and a changing climate INDEX Executive summary / 2 Company profile - Monsanto / 3 Opposition to Monsanto in Europe / 5 A decade of French resistance to GMOs / 6 Spanish movements against GM crops / 9 German farmers’ movement for GM-free regions / 10 Organising a movement for food sovereignty in Europe / 10 Monsanto, Quit India! / 11 Bt brinjal and biopiracy / 11 Bt cotton dominates cotton sector / 12 Spiralling debt still triggering suicides / 12 Stopping Monsanto’s new public-private partnerships / 13 Resistance to Monsanto in Latin America / 14 Brazilian peasant farmers’ movement against agribusiness / 14 Ten-year moratorium on GM in Peru / 15 Landmark ruling on toxic soy in Argentina / 15 Haitians oppose seed aid / 16 Guatemalan networks warn of new biosafety proposals / 17 Battle-lines drawn in the United States / 17 Stopping the -
General Court of the European Union PRESS RELEASE No 160/13 Luxembourg, 13 December 2013
General Court of the European Union PRESS RELEASE No 160/13 Luxembourg, 13 December 2013 Judgment in Case T-240/10 Press and Information Hungary v Commission The General Court has annulled the Commission’s decisions concerning authorisation to place on the market the genetically modified potato Amflora The Commission infringed the procedural rules of the systems for authorising GMOs in the European Union In the territory of the European Union, genetically modified organisms (GMOs) may be released into the environment or placed on the market only if consent has been given, subject to specific conditions and granted with a view to specified uses, after a scientific assessment of the risks. The authorisation system consists of two different procedures which are applied depending on the intended use of the GMOs. The aim of the first procedure, whose rules are laid down by Directive 2001/18/EC1, is to authorise GMOs with a view to their deliberate release into the environment. Within the framework of that procedure, it is in principle for the Member State with which an undertaking has lodged an application for this purpose to issue consent. However, the other Member States, and also the Commission, may raise objections vis-à-vis the intended consent decision. The second authorisation procedure, set up by Regulation 1829/20032, concerns genetically modified food and feed. In that case, the application for consent is assessed at EU level. Where, in the context of the first procedure, an objection has been raised or, in the context of the second procedure, an application for consent has been submitted, the final decision on the authorisation is taken by the Commission or by the Council on the basis of the scientific opinions of the European Food Safety Authority (EFSA). -
The Deadlock in European GM Crop Authorisations As a Wicked Problem by Design
The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Ruth Mampuys The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Ruth Mampuys Colofon Sociology, Theory and Methodology | Erasmus School of Law | 2020 Author: Ruth Mampuys Thesis design & layout: Bart Erkamp Cover design: Matteo Bettoni The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Thesis To obtain the degree of Doctor from the Erasmus University Rotterdam By command of the rector magnificus Prof.dr. F.A. van der Duijn Schouten and in accordance with the decision of the Doctorate Board. The public defence shall be held on Thursday 28 january 2021 at 15:30 hrs by Ruth Mampuys born in Enschede, the Netherlands Doctoral Committee Promotors: Prof. dr. W. van der Burg Prof. dr. F.W.A. Brom Other members: Prof. dr. A. Arcuri Prof. dr. K. Millar Prof. dr. J.E.J. Prins Copromotor: Dr. L.M. Poort CONTENTS PREFACE 1 LIST OF ABBREVIATIONS AND ACRONYMS 5 CHAPTER 1 Biotechnology governance: why, how and by whom? 9 1. Introduction 11 2. Varying definitions of biotechnology and GMOs 14 3. Recurring themes in discussions about biotechnology 17 3.1 Fundamental moral perspectives 18 3.2 Attitudes on risks/benefits 19 3.3 Broader issues 20 4. Regulatory framework for GMOs in Europe 21 4.1 Prerequisite: an environmental risk and food safety assessment 24 4.2 Regulatory decision-making: Comitology 25 4.3 Decision-making in practice 30 5. -
Bt Cotton in Texas
B-6107 02-01 Bt Cotton Technology in Texas: A Practical View Glen C. Moore, Thomas W. Fuchs, Mark A. Muegge, Allen E. Knutson* Since their introduction in 1996, transgenic cot- tons expressing the Bollgard® gene technology have been evaluated by producers in large scale commer- cial plantings across the U.S. Cotton Belt. Transgenic cottons are designed to be resistant to the target pests of bollworm Helicoverpa zea (Boddie), pink bollworm Pectinophora gossypiella (Sanders), and tobacco budworm Heliothis virescens (F.). These cottons contain Bacillus thuringiensis (Bt), a gene toxic to the target pests. The perfor- mance of these cottons has been highly efficacious against the tobacco budworm and the pink bollworm. They also preform well against bollworm; however, in certain situations producers may need to make supplemental insecticide treatments for this insect. Conditions that have contributed to the need for sup- plemental control are heavy bollworm egg laying during peak bloom, boll injury and the presence of larvae larger than 1/4 inch, high production inputs that favor rapid or rank plant growth, and fields pre- viously treated with insecticides. Earliest reports of bollworm damage on transgenic cotton varieties NuCOTN 33B and NuCOTN 35B surfaced in the Brazos Bottomlands and parts of the upper Coastal Bend areas of Texas in 1996. NuCOTN 33B and NuCOTN 35B have, however, provided effective bollworm control throughout much of Texas and reduced insecticide treatments for bollworm, tobacco budworm and pink bollworm compared to non-Bollgard® cotton. Yields from Bollgard® cotton are generally equal to or slightly higher than those for standard non- Bollgard® cultivars grown under the same production scheme. -
Lay and Scientific Categorizations of New Breeding Techniques: Implications for Food Policy and Genetically Modified Organism Legislation
1 Public Understanding of Science Archimer July 2020, Volume 29, Issue 5, Pages 524-543 https://doi.org/10.1177/0963662520929668 https://archimer.ifremer.fr https://archimer.ifremer.fr/doc/00634/74604/ Lay and scientific categorizations of new breeding techniques: Implications for food policy and genetically modified organism legislation Debucquet Gervaise 1, * , Baron Regis 2, Cardinal Mireille 3 1 AUDENCIA Business School, France 2 Unité Biotechnologies et Ressources Marines, IFREMER, Rue de l’Ile d’Yeu, France 3 Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies (EM3B), IFREMER, Rue de l’Ile d’Yeu, France * Corresponding author : Gervaise Dubucquet, email address : [email protected] Abstract : The rapid development of new genetic breeding techniques is accompanied by a polarized debate around their risks. Research on the public perception of these techniques lags behind scientific developments. This study tests a method for revealing laypeople’s perceptions and attitudes about different genetic techniques. The objectives are to enable laypeople to understand the key principles of new genetic breeding techniques and to permit a comparison of their modes of classification with those of scientific experts. The combined method of a free sorting task and focus groups showed that the participants distinguished the techniques that did not induce any change in DNA sequence, and applied two different logics to classify the other breeding techniques: a Cartesian logic and a naturalistic logic with a distinct set of values. The lay categorization differed substantially from current scientific categorizations of genetic breeding techniques. These findings have implications for food innovation policy and genetically modified organism legislation. Keywords : food policy, genetically modified organism regulation, genetically modified organisms, lay categorization, new breeding techniques, and public understanding Please note that this is an author-produced PDF of an article accepted for publication following peer review. -
Barriers to Adoption of GM Crops
Iowa State University Capstones, Theses and Creative Components Dissertations Fall 2021 Barriers to Adoption of GM Crops Madeline Esquivel Follow this and additional works at: https://lib.dr.iastate.edu/creativecomponents Part of the Agricultural Education Commons Recommended Citation Esquivel, Madeline, "Barriers to Adoption of GM Crops" (2021). Creative Components. 731. https://lib.dr.iastate.edu/creativecomponents/731 This Creative Component is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Creative Components by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Barriers to Adoption of GM Crops By Madeline M. Esquivel A Creative Component submitted to the Graduate Faculty in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Major: Plant Breeding Program of Study Committee: Walter Suza, Major Professor Thomas Lübberstedt Iowa State University Ames, Iowa 2021 1 Contents 1. Introduction ................................................................................................................................. 3 2. What is a Genetically Modified Organism?................................................................................ 9 2.1 The Development of Modern Varieties and Genetically Modified Crops .......................... 10 2.2 GM vs Traditional Breeding: How Are GM Crops Produced? -
Metabolic Engineering Using Iterative Self-Cloning to Improve Lipid
www.nature.com/scientificreports OPEN Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa Received: 14 December 2017 Yuki Kasai1,2, Takuya Tsukahara1, Fukiko Ikeda1, Yoko Ide1,2,3 & Shigeaki Harayama1,2 Accepted: 26 July 2018 We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate Published: xx xx xxxx synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura−) of the same alga. Here, we developed a Cre/loxP-based system for the removal of cUMPS fanked by directly repeated loxP sites from the Coccomyxa genome using the intracellular delivery of purifed Cre recombinase to generate an Ura− strain that was used as a host for second-round transformation using cUMPS as the selection marker. Employing this marker–gene-recycling system, Coccomyxa strains devoid of foreign DNA except the 34-bp loxP sequence, which overexpressed an acyl-(acyl-carrier-protein) thioesterase gene, and a type-2 diacylglycerol acyltransferase gene, were constructed by the sequential introduction of two expression cassettes for the respective genes. One of the resulting strains showed 1.4-fold higher lipid productivity than the wild-type strain. This method will be applicable to other eukaryotic microalgae to create marker-free transgenic strains. Increase in greenhouse gas emissions and associated global warming has led to growing interest in the develop- ment and production of biofuels. Microalgae have long been recognized as a potential source for biofuel produc- tion because of high oil content, high growth rate, and non-requirement of arable land for cultivation1. -
Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton Bollworm
Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton Bollworm Haonan Zhang1, Shuwen Wu1, Yihua Yang1, Bruce E. Tabashnik2, Yidong Wu1* 1 Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China, 2 Department of Entomology, University of Arizona, Tucson, Arizona, United States of America Abstract Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach. Recessive mutations in the extracellular domain of toxin- binding cadherin proteins that confer resistance to Bt toxin Cry1Ac by disrupting toxin binding have been reported previously in three major lepidopteran pests, including the cotton bollworm, Helicoverpa armigera. Here we report a novel allele from cotton bollworm with a deletion in the intracellular domain of cadherin that is genetically linked with non- recessive resistance to Cry1Ac. We discovered this allele in each of three field-selected populations we screened from northern China where Bt cotton producing Cry1Ac has been grown intensively. We expressed four types of cadherin alleles in heterologous cell cultures: susceptible, resistant with the intracellular domain mutation, and two complementary chimeric alleles with and without the mutation. Cells transfected with each of the four cadherin alleles bound Cry1Ac and were killed by Cry1Ac. However, relative to cells transfected with either the susceptible allele or the chimeric allele lacking the intracellular domain mutation, cells transfected with the resistant allele or the chimeric allele containing the intracellular domain mutation were less susceptible to Cry1Ac. -
Monsanto's BT Cotton Patent, Indian Courts and Public Policy
6. MONSANTO’S BT COTTON PATENT, INDIAN COURTS AND eligible subject matter promotes public policy and farmers’ PUBLIC POLICY interests. Keywords: Bt. cotton, patent eligible subject matter, nucleic Ghayur Alam∗ acid sequence, plant, public policy, revocation of patent, seed, ABSTRACT TRIPS. This Paper primarily deals with an unanswered substantial 1. INTRODUCTION question of patent law that has arisen in India. The question is whether an invented Nucleic Acid Sequence after being A substantial question of patent law (hereinafter, ‘question’) inserted into a seed or plant becomes part of the seed or the of great significance has arisen in India and is awaiting an plant. If answer is in the affirmative, said invention is not answer from Indian courts. The question is whether an patentable under Section 3(j) of the (Indian) Patents Act 1970, invented Nucleic Acid Sequence (NAS) after being inserted which excludes from patentability, inter alia, plants, seeds, or into a seed or plant becomes part of the seed or the plant? any part thereof. If answer is in the negative, said invention is Specifically, the question is whether the patent granted1 by patentable. Answer will determine the fate of patenting of the Indian Patent Office to Monsanto Technology LLC such inventions in the field of agro-biotechnology. Problem is (Monsanto) on transgenic variety of cottonseeds, containing that the question has moved forth and back like pendulum invented Bt. trait is valid or not? If the answer is in affirmative, from one court to another but in vain. This paper seeks to claimed invention is not patentable under the (Indian) address this question in light of the decisions of Indian courts. -
SYMPOSIUM TARRAGONA 1 St / 4 Th APRIL 2019
1 5th SYMPOSIUM TARRAGONA 1 st / 4 th APRIL 2019 BOOK OF ABSTRACTS WELCOME Dear colleagues It is a pleasure to welcome you all to the 15th ISBR Symposium. Our program committee has worked tirelessly to put together an interesting and exciting scientific programme. Many volunteers have helped us to organise an array of parallel sessions and workshops that cover many of the scientific topics currently under intense discussion in our scientific community. We are also very honoured to have with us an outstanding group of keynote and plenary speakers. We hope that with such a program you will all find plenty of opportunities to hear about the latest scientific developments in this field, to learn new things and to interact with other scientists, widening your network and fostering new collaborations. We have chosen the beautiful city of Tarragona to host this Symposium for many reasons. With the stunning Mediterranean Sea as the backdrop, the many historical Roman sites and its laid back nature, Tarragona provides a wonderful venue to engage in the Symposium at the Palau Firal and then relax and enjoy its many offerings when the sessions are over. We warmly welcome you and sincerely hope that you enjoy this Symposium and have a pleasant stay in Tarragona. Dr. Ariel Alvarez Dr. Monica Garcia-Alonso ISBR President Symposium coordinator and local organiser 15th ISBR Symposium 2019 TARRAGONA · 1st - 4th APRIL 2 SYMPOSIUM ORGANIZATION SYMPOSIUM COORDINATOR / LOCAL ORGANISER ISBR BOARD MÓNICA GARCÍA-ALONSO ARIEL ALVAREZ-MORALES (ISBR President) > Estel Consult Ltd / ISBR > Center for Research and Advances Studies – CINVESTAV RALF WILHELM SCIENTIFIC PROGRAM COMMITTEE > Julius Kühn-Institut MORVEN A.