For research use only only use research For Measurement of h Cathepsin S of h Cathepsin Measurement activity by Cathepsin S Activity Assay Kit (K144 100). (K144 100). Kit Assay
(Cat. No. 7277-10, -50, -1000) -1000) -50, 7277-10, No. (Cat.
(Cat. No. 7600-5, -50) 7600-5, No. (Cat. (Cat. No. 7580-5, -50, -1000) -50, 7580-5, No. (Cat. (Cat. No. 7597-5, -50) 7597-5, No. (Cat. (Cat. No. 1030-1) 1030-1) No. (Cat. (Cat. No. 7598-5, -50) 7598-5, No. (Cat. (Cat. No. 7579-10, -50, -1000) -1000) -50, 7579-10, No. (Cat. (Cat. No. 7525-10, -50, -500) -500) -50, 7525-10, No. (Cat. (Cat. No. 7581-5, -100, -1000) -1000) -100, 7581-5, No. (Cat. SDS PAGE (15%) of h Cathepsin S: S: of h Cathepsin (15%) SDS PAGE Marker 1: Protein g) (5 S 2: h Cathepsin g) (10 S 3: h Cathepsin g) (20 S 4: h Cathepsin (Cat. No. 1022-5) No. (Cat. (Cat. No. 1023-5) No. (Cat. (Cat. No. 1021-5) No. (Cat.
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human) (active, D Cathepsin recombinant rat Active, K, Cathepsin human) (active, H Cathepsin human) B (active, Cathepsin recombinant mouse Active, K, Cathepsin buffer activation Procathepsin EZAct™ recombinant human Active, K, Cathepsin recombinant human L, Procathepsin recombinant human Active, B, Cathepsin recombinant human B, Procathepsin recombinant human S, Cathepsin CellExp™ Human recombinant human S, Procathepsin • • • • • • • • • • • •
PRODUCTS: RELATED humans. to be in used Not Use For Research Only!
08/14
VVR AFC VVR AFC
n down the auto catalytic of the papain family eening, eening, kinetic studies
lycosylation lycosylation site located in the g ive ive precursor of 331 amino acids , , L and H, cathepsin S shows a g g g g µ µ µ id id beta peptide from amyloidogenic mM NaCl (pH 5.5) to 0.1 1 mg/ml and proteins to peptides for presentation on endosomal/lysosomal compartment, and compartment, endosomal/lysosomal . The enzyme is fully active as seen from of 99 aa, and a mature ofpolypeptide 217 in spleen, heart, and lung. In addition, sease and Down Syndrome. Syndrome. Down and sease , CA 95035 USA USA 95035 CA , ic ic cleavage of the propeptide. The deduced AFC (K144 100). AFC
or in tissue culture. culture. tissue in or Lyophilized from proprietary buffer. from proprietary Lyophilized CTSS CTSS coli E. 7526 500 7526 500 500 7526 50 7526 50 50 7526 10 7526 10 10 aa) (115 331 kDa 23.9 Stable for at least 1 year as supplied. Briefly spi >90% by SDS PAGE. SDS PAGE. by >90% in vitro in
Incorporated BioVision >2000 >2000 mU/mg (1 U = Digestion of 1 mole/min) of Ac
BioVision’s BioVision’s Active Cathepsin S has been prepared by Active Active human Cathepsin S is useful in inhibitor scr Cathepsin S (CTSS) is a cysteine lysosomal protease C. Avoid repeated freeze and thaw cycles. cycles. thaw and freeze repeated Avoid C. ° Lyophilized.
FORM: FORMULATION: SOURCE: SOURCE: ALTERNATIVE NAMES: NAMES: ALTERNATIVE vial vial and reconstitute in 50 mM sodium acetate, 100 –80 store at STORAGE CONDITIONS: CONDITIONS: STORAGE human S, Active, Cathepsin recombinant #: CATALOG WT.: MOL. BACKGROUND: and and may participate in the degradation of antigenic MHC class II molecules. CTSS is synthesized as consisting of a 15 aa signal inact peptide, a propeptide aa. It is in activated the by lysosomes a proteolyt amino acid sequence contains propeptide. only Compared with one the potential abundant cathepsins N g B restricted tissue distribution, with highest levels evidences indicate that cathepsin S generates amylo (APP) in of fragments protein the amyloid precursor di Alzheimer’s of the pathogenesis in implicated is ASSAY: ACTIVITY activation of Procathepsin activation Sof Procathepsin (Cat # 7525) at low pH Z VVR substrate a fluorogenic cleave to its ability and cleavage of substrates substrates of cleavage and SPECIFIC SPECIFIC ACTIVITY: substrate (K144 100). substrate APPLICATIONS: APPLICATIONS: PURITY: PURITY: BioVision BioVision Milpitas Boulevard, Milpitas S. 155