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Download Enlarge Produktinformation Diagnostik & molekulare Diagnostik Laborgeräte & Service Zellkultur & Verbrauchsmaterial Forschungsprodukte & Biochemikalien Weitere Information auf den folgenden Seiten! See the following pages for more information! Lieferung & Zahlungsart Lieferung: frei Haus Bestellung auf Rechnung SZABO-SCANDIC Lieferung: € 10,- HandelsgmbH & Co KG Erstbestellung Vorauskassa Quellenstraße 110, A-1100 Wien T. +43(0)1 489 3961-0 Zuschläge F. +43(0)1 489 3961-7 [email protected] • Mindermengenzuschlag www.szabo-scandic.com • Trockeneiszuschlag • Gefahrgutzuschlag linkedin.com/company/szaboscandic • Expressversand facebook.com/szaboscandic WDR58 monoclonal antibody (M01), clone 1F6 Catalog # : H00079228-M01 規格 : [ 100 ug ] List All Specification Application Image Product Mouse monoclonal antibody raised against a partial recombinant Western Blot (Cell lysate) Description: WDR58. Immunogen: WDR58 (NP_077315, 71 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence: TFQAHDGPVYSMVSTDRHLLSAGDGEVKAWLWAEMLKKGCKELWRRQ PPYRTSLEVPEINALLLVPKENSLILAGGDCQLHTMDLETGTFTRVLRGH enlarge TDYIHCLALRERS Western Blot (Cell lysate) Host: Mouse Reactivity: Human, Rat Isotype: IgG1 Kappa enlarge Quality Control Antibody Reactive Against Recombinant Protein. Testing: Western Blot (Transfected lysate) enlarge Western Blot (Recombinant protein) Immunohistochemistry Western Blot detection against Immunogen (37.84 KDa) . (Formalin/PFA-fixed paraffin- embedded sections) Storage Buffer: In 1x PBS, pH 7.4 Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Instruction: MSDS: Download enlarge Datasheet: Download Sandwich ELISA (Recombinant protein) Publication Reference 1. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa YNucleic Acids Res. 2013 Aug;41(14):7060-72. doi: 10.1093/nar/gkt414. Epub 2013 May 17. 2. Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of enlarge HSP70 mRNA. Katahira J, Inoue H, Hurt E, Yoneda Y.EMBO J. 2009 Mar 4;28(5):556-67. Epub 2009 ELISA Jan 22. Applications Page 1 of 3 2020/7/13 Western Blot (Cell lysate) WDR58 monoclonal antibody (M01), clone 1F6. Western Blot analysis of WDR58 expression in PC-12 ( Cat # L012V1 ). Protocol Download Western Blot (Cell lysate) WDR58 monoclonal antibody (M01), clone 1F6 Western Blot analysis of WDR58 expression in A-431 ( Cat # L015V1 ). Protocol Download Western Blot (Transfected lysate) Western Blot analysis of THOC6 expression in transfected 293T cell line by THOC6 monoclonal antibody (M01), clone 1F6. Lane 1: THOC6 transfected lysate (Predicted MW: 37.5 KDa). Lane 2: Non-transfected lysate. Protocol Download Western Blot (Recombinant protein) Protocol Download Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Page 2 of 3 2020/7/13 enlarge this image Immunoperoxidase of monoclonal antibody to WDR58 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Protocol Download Sandwich ELISA (Recombinant protein) Detection limit for recombinant GST tagged WDR58 is approximately 0.3ng/ml as a capture antibody. Protocol Download ELISA Gene Information Entrez GeneID: 79228 GeneBank NM_024339 Accession#: Protein NP_077315 Accession#: Gene Name: THOC6 Gene Alias: MGC2655,WDR58,fSAP35 Gene THO complex 6 homolog (Drosophila) Description: Gene Ontology: Hyperlink Other WD repeat domain 58,functional spliceosome-associated protein 35 Designations: Related Disease Cardiovascular Diseases Diabetes Mellitus, Type 2 Edema Genetic Predisposition to Disease Hematologic Diseases Occupational Diseases 服務條款 | 隱私權政策 | 著作及商標 | 網站地圖 ©2020 亞諾法生技股份有限公司 Abnova Corporation. 版權所有. Page 3 of 3 2020/7/13.
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  • Integrated Bioinformatic Analysis of RNA Binding Proteins in Hepatocellular Carcinoma
    www.aging-us.com AGING 2021, Vol. 13, No. 2 Research Paper Integrated bioinformatic analysis of RNA binding proteins in hepatocellular carcinoma Ling Wang1,*, Zhen Zhang2,3,*, Yuan Li1, Yanyan Wan1, Baocai Xing1,& 1Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Hepatopancreatobiliary Surgery Department I, Peking University Cancer Hospital and Institute, Beijing 100142, China 2Department of Gastroenterological Surgery, Peking University People’s Hospital, Beijing 100044, China 3Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing 100044, China *Equal contribution Correspondence to: Baocai Xing; email: [email protected] Keywords: RNA binding protein, hepatocellular carcinoma, biomarker, transcriptomics, proteomics Received: June 8, 2020 Accepted: November 3, 2020 Published: December 19, 2020 Copyright: © 2020 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT RNA binding proteins (RBPs) are aberrantly expressed in a tissue-specific manner across many tumors. These proteins, which play a vital role in post-transcriptional gene regulation, are involved in RNA splicing, maturation, transport, stability, degradation, and translation. We set out to establish an accurate risk score model based on RBPs to estimate prognosis in hepatocellular carcinoma (HCC). RNA-sequencing data, proteomic data and corresponding clinical information were acquired from the Cancer Genome Atlas database and the Clinical Proteomic Tumor Analysis Consortium database respectively. We identified 406 differentially expressed RBPs between HCC tumor and normal tissues at the transcriptional and protein level.
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